15 results on '"Liu, Hailan"'
Search Results
2. Loss of transient receptor potential channel 5 causes obesity and postpartum depression
- Author
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Li, Yongxiang, Cacciottolo, Tessa M., Yin, Na, He, Yang, Liu, Hesong, Liu, Hailan, Yang, Yuxue, Henning, Elana, Keogh, Julia M., Lawler, Katherine, Mendes de Oliveira, Edson, Gardner, Eugene J., Kentistou, Katherine A., Laouris, Panayiotis, Bounds, Rebecca, Ong, Ken K., Perry, John R.B., Barroso, Inês, Tu, Longlong, Bean, Jonathan C., Yu, Meng, Conde, Kristine M., Wang, Mengjie, Ginnard, Olivia, Fang, Xing, Tong, Lydia, Han, Junying, Darwich, Tia, Williams, Kevin W., Yang, Yongjie, Wang, Chunmei, Joss, Shelagh, Firth, Helen V., Xu, Yong, and Farooqi, I. Sadaf
- Published
- 2024
- Full Text
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3. Emerging Trends in Integrated Digital Microfluidic Platforms for Next-Generation Immunoassays.
- Author
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Su, Kaixin, Li, Jiaqi, Liu, Hailan, and Zou, Yuan
- Subjects
ACOUSTIC surface waves ,COMPLEX fluids ,IMMUNOASSAY ,DIGITAL technology ,POINT-of-care testing - Abstract
Technologies based on digital microfluidics (DMF) have made significant advancements in the automated manipulation of microscale liquids and complex multistep processes. Due to their numerous benefits, such as automation, speed, cost-effectiveness, and minimal sample volume requirements, these systems are particularly well suited for immunoassays. In this review, an overview is provided of diverse DMF manipulation platforms and their applications in immunological analysis. Initially, droplet-driven DMF platforms based on electrowetting on dielectric (EWOD), magnetic manipulation, surface acoustic wave (SAW), and other related technologies are briefly introduced. The preparation of DMF is then described, including material selection, fabrication techniques and droplet generation. Subsequently, a comprehensive account of advancements in the integration of DMF with various immunoassay techniques is offered, encompassing colorimetric, direct chemiluminescence, enzymatic chemiluminescence, electrosensory, and other immunoassays. Ultimately, the potential challenges and future perspectives in this burgeoning field are delved into. [ABSTRACT FROM AUTHOR]
- Published
- 2024
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4. 5-HT Neurons Integrate GABA and Dopamine Inputs to Regulate Meal Initiation
- Author
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Conde, Kristine, primary, Wong, HueyZhong, additional, Fang, Shuzheng, additional, Li, Yongxiang, additional, Yu, Meng, additional, Deng, Yue, additional, Liu, Qingzhuo, additional, Fang, Xing, additional, Wang, Mengjie, additional, Shi, Yuhan, additional, Ginnard, Olivia G., additional, Yang, Yuxue, additional, Tu, Longlong, additional, Liu, Hesong, additional, Liu, Hailan, additional, Yin, Na, additional, Bean, Jonathan C., additional, Han, Junying, additional, Burt, Megan E., additional, Jossy, Sanika V., additional, Yang, Yongjie, additional, Tong, Qingchun, additional, Arenkiel, Benjamin, additional, Wang, Chunmei, additional, He, Yang, additional, and Xu, Yong, additional
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- 2024
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5. Recent Advances in Magnetically Actuated Droplet Manipulation for Biomedical Applications
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Li, Jiaqi, primary, Su, Kaixin, additional, Liu, Hailan, additional, and Zou, Yuan, additional
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- 2024
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6. Therapeutic Strategies Against Metabolic Imbalance in a Male Mouse Model With 5-HT2CR Loss-of-Function.
- Author
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Liu, Hailan, Liu, Zhaoxun, Wong, HueyXian Kelly, Xu, Nathan, Liu, Qingzhuo, Li, Yongxiang, Liu, Yao, Wong, HueyZhong, Burt, Megan E, Jossy, Sanika V, Han, Junying, and He, Yang
- Subjects
MALE models ,LABORATORY mice ,MELANOCORTIN receptors ,ANIMAL disease models ,SEROTONIN receptors ,HYPERGLYCEMIA ,RUNNING injuries - Abstract
The serotonin 2C receptor (5-HT
2C R)-melanocortin pathway plays well-established roles in the regulation of feeding behavior and body weight homeostasis. Dysfunctions in this system, such as loss-of-function mutations in the Htr2c gene, can lead to hyperphagia and obesity. In this study, we aimed to investigate the potential therapeutic strategies for ameliorating hyperphagia, hyperglycemia, and obesity associated with a loss-of-function mutation in the Htr2c gene (Htr2cF327L/Y ). We demonstrated that reexpressing functional 5-HT2C R solely in hypothalamic pro-opiomelanocortin (POMC) neurons is sufficient to reduce food intake and body weight in Htr2cF327L/Y mice subjected to a high-fat diet (HFD). In addition, 5-HT2C R expression restores the responsiveness of POMC neurons to lorcaserin, a selective agonist for 5-HT2C R. Similarly, administration of melanotan II, an agonist of the melanocortin receptor 4 (MC4R), effectively suppresses feeding and weight gain in Htr2cF327L/Y mice. Strikingly, promoting wheel-running activity in Htr2cF327L/Y mice results in a decrease in HFD consumption and improved glucose homeostasis. Together, our findings underscore the crucial role of the melanocortin system in alleviating hyperphagia and obesity related to dysfunctions of the 5-HT2C R, and further suggest that MC4R agonists and lifestyle interventions might hold promise in counteracting hyperphagia, hyperglycemia, and obesity in individuals carrying rare variants of the Htr2c gene. [ABSTRACT FROM AUTHOR]- Published
- 2024
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- View/download PDF
7. Quantitative assessment of left ventricular myocardial work in patients with different types of atrial fibrillation by non‐invasive pressure‐strain loop technique.
- Author
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Liu, Tingting, Liu, Hailan, Song, Yan, Huang, Yulin, and Zhang, Chunquan
- Subjects
- *
MYOCARDIUM physiology , *LEFT heart ventricle , *RESEARCH funding , *T-test (Statistics) , *DATA analysis , *MULTIPLE regression analysis , *FISHER exact test , *HEART physiology , *DESCRIPTIVE statistics , *QUANTITATIVE research , *CHI-squared test , *MANN Whitney U Test , *ATRIAL fibrillation , *DOPPLER echocardiography , *ANALYSIS of variance , *STATISTICS , *DATA analysis software , *DISEASE complications - Abstract
Objective: This study aimed to analyze myocardial work in patients with atrial fibrillation (AF) using a noninvasive pressure strain loop (PSL) technique to provide a basis for the quantitative assessment of left ventricular (LV) systolic function. Methods: LV myocardial work of 107 AF patients (56 with paroxysmal atrial fibrillation and 51 with persistent atrial fibrillation) and 55 healthy individuals were assessed by the noninvasive PSL and then compared. Results: Global longitudinal strain (GLS) in absolute values, global work index (GWI), global constructive work (GCW), and global work efficiency (GWE) were significantly lower in the AF group than control group, whereas peak strain dispersion (PSD) and global wasted work (GWW) were significantly higher (P <.05). Further subdivision according to the AF type revealed that, compared with the controls, GLS in absolute values and GWE decreased significantly; PSD and GWW increased significantly in the paroxysmal AF group (P <.05). Nevertheless, GWI and GCW were not significantly different between paroxysmal AF and control groups (P >.05). Compared to paroxysmal AF, persistent AF induced a further decrease in absolute GLS and GWE and a further increase in GWW (P <.05), but PSD did not increase further (P >.05). Multiple linear regression analysis showed that GWI and GCW were independently associated with systolic blood pressure. GWW was associated with types of AF and left atrial volume index (LAVI). GWE was correlated with age, types of AF, disease duration, and LAVI. Receiver operating characteristic curve analysis showed that the area under the curve predicting myocardial injury was higher for GWE and GWW than for GLS (area under the curve:.880,.846, and.821, respectively). Conclusions: Non‐invasive PSL can quantitatively assess LV systolic function in patients with different kinds of AF and detect early subclinical myocardial injury in patients with paroxysmal AF. GWE and GWW outperform GLS and LV ejection fraction when assessing myocardial injury. Systolic blood pressure, type of AF, LVAI, disease duration, and age may be associated with myocardial injury in patients with AF. [ABSTRACT FROM AUTHOR]
- Published
- 2024
- Full Text
- View/download PDF
8. Therapeutic Strategies Against Metabolic Imbalance in a Male Mouse Model With 5-HT2CR Loss-of-Function
- Author
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Liu, Hailan, Liu, Zhaoxun, Wong, HueyXian Kelly, Xu, Nathan, Liu, Qingzhuo, Li, Yongxiang, Liu, Yao, Wong, HueyZhong, Burt, Megan E, Jossy, Sanika V, Han, Junying, and He, Yang
- Abstract
The serotonin 2C receptor (5-HT2CR)-melanocortin pathway plays well-established roles in the regulation of feeding behavior and body weight homeostasis. Dysfunctions in this system, such as loss-of-function mutations in the Htr2cgene, can lead to hyperphagia and obesity. In this study, we aimed to investigate the potential therapeutic strategies for ameliorating hyperphagia, hyperglycemia, and obesity associated with a loss-of-function mutation in the Htr2cgene (Htr2cF327L/Y). We demonstrated that reexpressing functional 5-HT2CR solely in hypothalamic pro-opiomelanocortin (POMC) neurons is sufficient to reduce food intake and body weight in Htr2cF327L/Ymice subjected to a high-fat diet (HFD). In addition, 5-HT2CR expression restores the responsiveness of POMC neurons to lorcaserin, a selective agonist for 5-HT2CR. Similarly, administration of melanotan II, an agonist of the melanocortin receptor 4 (MC4R), effectively suppresses feeding and weight gain in Htr2cF327L/Ymice. Strikingly, promoting wheel-running activity in Htr2cF327L/Ymice results in a decrease in HFD consumption and improved glucose homeostasis. Together, our findings underscore the crucial role of the melanocortin system in alleviating hyperphagia and obesity related to dysfunctions of the 5-HT2CR, and further suggest that MC4R agonists and lifestyle interventions might hold promise in counteracting hyperphagia, hyperglycemia, and obesity in individuals carrying rare variants of the Htr2cgene.
- Published
- 2024
- Full Text
- View/download PDF
9. Efficient large-scale genomic prediction in approximate genome-based kernel model.
- Author
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Liu H, Xu J, Wang X, Wang H, Wang L, and Shen Y
- Subjects
- Computer Simulation, Genome, Plant, Algorithms, Models, Genetic, Genomics methods
- Abstract
Key Message: Three computationally efficient algorithms of GP including RHBK, RHDK, and RHPK were developed in approximate genome-based kernel model. The drastically growing amount of genomic information contributes to increasing computational burden of genomic prediction (GP). In this study, we developed three computationally efficient algorithms of GP including RHBK, RHDK, and RHPK in approximate genome-based kernel model, which reduces dimension of genomic data via Nyström approximation and decreases the computational cost significantly thereby. According to the simulation study and real datasets, our three methods demonstrated predictive accuracy similar to or better than RHAPY, GBLUP, and rrBLUP in most cases. They also demonstrated a substantial reduction in computational time compared to GBLUP and rrBLUP in simulation. Due to their advanced computing efficiency, our three methods can be used in a wide range of application scenarios in the future., Competing Interests: Declarations. Conflict of interest: The authors declare that they have no conflict of interest. Ethical approval: Not applicable., (© 2024. The Author(s), under exclusive licence to Springer-Verlag GmbH Germany, part of Springer Nature.)
- Published
- 2024
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10. Serotonin neurons integrate GABA and dopamine inputs to regulate meal initiation.
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Conde KM, Wong H, Fang S, Li Y, Yu M, Deng Y, Liu Q, Fang X, Wang M, Shi Y, Ginnard OZ, Yang Y, Tu L, Liu H, Liu H, Yin N, Bean JC, Han J, Burt ME, Jossy SV, Yang Y, Tong Q, Arenkiel BR, Wang C, He Y, and Xu Y
- Abstract
Obesity is a growing global health epidemic with limited orally administered therapeutics. Serotonin (5-HT) is one neurotransmitter which remains an excellent target for new weight-loss therapies, but a gap remains in understanding the mechanisms involved in 5-HT produced in the dorsal Raphe nucleus (DRN) and its involvement in meal initiation. Using an optogenetic feeding paradigm, we showed that the 5-HT
DRN ➔arcuate nucleus (ARH) circuit plays a role in meal initiation. Incorporating electrophysiology and ChannelRhodopsin-2-Assisted Circuit Mapping, we demonstrated that 5-HTDRN neurons receive inhibitory input partially from GABAergic neurons in the DRN, and the 5-HT response can be enhanced by hunger. Additionally, deletion of the GABAA receptor subunit in 5-HT neurons inhibits meal initiation with no effect on the satiation process. Finally, we identified the role of dopaminergic inputs via dopamine receptor D2 in enhancing the response to GABA-induced feeding. Thus, our results indicate that 5-HTDRN neurons are inhibited by synergistic inhibitory actions of GABA and dopamine, for the initiation of a meal., Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2024 The Authors. Published by Elsevier Inc. All rights reserved.)- Published
- 2024
- Full Text
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11. Identification of an ionic mechanism for ERα-mediated rapid excitation in neurons.
- Author
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Yu M, Yin N, Feng B, Gao P, Yu K, Liu H, Liu H, Li Y, Ginnard OZ, Conde KM, Wang M, Fang X, Tu L, Bean JC, Liu Q, Deng Y, Yang Y, Han J, Jossy SV, Burt ML, Wong HZ, Yang Y, Arenkiel BR, He Y, Guo S, Gourdy P, Arnal JF, Lenfant F, Wang Z, Wang C, He Y, and Xu Y
- Subjects
- Animals, Female, Humans, Estradiol metabolism, Estradiol pharmacology, Mice, Hypothalamus metabolism, Hypothalamus cytology, Protein Binding, Neurons metabolism, Chloride Channels metabolism, Chloride Channels genetics, Estrogen Receptor alpha metabolism, Estrogen Receptor alpha genetics
- Abstract
The major female ovarian hormone, 17β-estradiol (E
2 ), can alter neuronal excitability within milliseconds to regulate a variety of physiological processes. Estrogen receptor-α (ERα), classically known as a nuclear receptor, exists as a membrane-bound receptor to mediate this rapid action of E2 , but the ionic mechanisms remain unclear. Here, we show that a membrane channel protein, chloride intracellular channel protein-1 (Clic1), can physically interact with ERα with a preference to the membrane-bound ERα. Clic1-mediated currents can be enhanced by E2 and reduced by its depletion. In addition, Clic1 currents are required to mediate the E2 -induced rapid excitations in multiple brain ERα populations. Further, genetic disruption of Clic1 in hypothalamic ERα neurons blunts the regulations of E2 on female body weight balance. In conclusion, we identified the Clic1 chloride channel as a key mediator for E2 -induced rapid neuronal excitation, which may have a broad impact on multiple neurobiological processes regulated by E2 .- Published
- 2024
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12. Genomic prediction of yield-related traits and genome-based establishment of heterotic pattern in maize hybrid breeding of Southwest China.
- Author
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Xiang Y, Xia C, Li L, Wei R, Rong T, Liu H, and Lan H
- Abstract
When genomic prediction is implemented in breeding maize ( Zea mays L.), it can accelerate the breeding process and reduce cost to a large extent. In this study, 11 yield-related traits of maize were used to evaluate four genomic prediction methods including rrBLUP, HEBLP|A, RF, and LightGBM. In all the 11 traits, rrBLUP had similar predictive accuracy to HEBLP|A, and so did RF to LightGBM, but rrBLUP and HEBLP|A outperformed RF and LightGBM in 8 traits. Furthermore, genomic prediction-based heterotic pattern of yield was established based on 64620 crosses of maize in Southwest China, and the result showed that one of the parent lines of the top 5% crosses came from temp-tropic or tropic germplasm, which is highly consistent with the actual situation in breeding, and that heterotic pattern (Reid+ × Suwan+) will be a major heterotic pattern of Southwest China in the future., Competing Interests: The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest., (Copyright © 2024 Xiang, Xia, Li, Wei, Rong, Liu and Lan.)
- Published
- 2024
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13. A Light-Responsive Neural Circuit Suppresses Feeding.
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Liu H, Qu N, Gonzalez NV, Palma MA, Chen H, Xiong J, Choubey A, Li Y, Li X, Yu M, Liu H, Tu L, Zhang N, Yin N, Conde KM, Wang M, Bean JC, Han J, Scarcelli NA, Yang Y, Saito K, Cui H, Tong Q, Sun Z, Wang C, Cai X, Lu L, He Y, and Xu Y
- Subjects
- Animals, Male, Mice, Dorsal Raphe Nucleus physiology, Humans, Mice, Inbred C57BL, Eating physiology, Neural Pathways physiology, Rats, Serotonergic Neurons physiology, Nerve Net physiology, Darkness, Habenula physiology, Feeding Behavior physiology, Light
- Abstract
Light plays an essential role in a variety of physiological processes, including vision, mood, and glucose homeostasis. However, the intricate relationship between light and an animal's feeding behavior has remained elusive. Here, we found that light exposure suppresses food intake, whereas darkness amplifies it in male mice. Interestingly, this phenomenon extends its reach to diurnal male Nile grass rats and healthy humans. We further show that lateral habenula (LHb) neurons in mice respond to light exposure, which in turn activates 5-HT neurons in the dorsal Raphe nucleus (DRN). Activation of the LHb→5-HT
DRN circuit in mice blunts darkness-induced hyperphagia, while inhibition of the circuit prevents light-induced anorexia. Together, we discovered a light-responsive neural circuit that relays the environmental light signals to regulate feeding behavior in mice., Competing Interests: The authors declare no competing financial interests., (Copyright © 2024 the authors.)- Published
- 2024
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14. Neural circuits expressing the serotonin 2C receptor regulate memory in mice and humans.
- Author
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Liu H, He Y, Liu H, Brouwers B, Yin N, Lawler K, Keogh JM, Henning E, Lee DK, Yu M, Tu L, Zhang N, Conde KM, Han J, Yan Z, Scarcelli NA, Liao L, Xu J, Tong Q, Zheng H, Sun Z, Yang Y, Wang C, He Y, Farooqi IS, and Xu Y
- Subjects
- Animals, Humans, Mice, Hippocampus metabolism, Hippocampus drug effects, Serotonin metabolism, Disease Models, Animal, CA1 Region, Hippocampal metabolism, CA1 Region, Hippocampal drug effects, Neurons metabolism, Neurons drug effects, Serotonin 5-HT2 Receptor Agonists pharmacology, Receptor, Serotonin, 5-HT2C metabolism, Receptor, Serotonin, 5-HT2C genetics, Memory drug effects, Memory physiology, Mice, Transgenic, Neuronal Plasticity drug effects, Alzheimer Disease metabolism
- Abstract
Declined memory is a hallmark of Alzheimer's disease (AD). Experiments in rodents and human postmortem studies suggest that serotonin (5-hydroxytryptamine, 5-HT) plays a role in memory, but the underlying mechanisms are unknown. Here, we investigate the role of 5-HT 2C receptor (5-HT
2C R) in regulating memory. Transgenic mice expressing a humanized HTR2C mutation exhibit impaired plasticity of hippocampal ventral CA1 (vCA1) neurons and reduced memory. Further, 5-HT neurons project to and synapse onto vCA1 neurons. Disruption of 5-HT synthesis in vCA1-projecting neurons or deletion of 5-HT2C Rs in the vCA1 impairs neural plasticity and memory. We show that a selective 5-HT2C R agonist, lorcaserin, improves synaptic plasticity and memory in an AD mouse model. Cumulatively, we demonstrate that hippocampal 5-HT2C R signaling regulates memory, which may inform the use of 5-HT2C R agonists in the treatment of dementia.- Published
- 2024
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15. 5-HT Neurons Integrate GABA and Dopamine Inputs to Regulate Meal Initiation.
- Author
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Conde KM, Wong H, Fang S, Li Y, Yu M, Deng Y, Liu Q, Fang X, Wang M, Shi Y, Ginnard OZ, Yang Y, Tu L, Liu H, Liu H, Yin N, Bean JC, Han J, Burt ME, Jossy SV, Yang Y, Tong Q, Arenkiel BR, Wang C, He Y, and Xu Y
- Abstract
Obesity is a growing global health epidemic with limited effective therapeutics. Serotonin (5-HT) is one major neurotransmitter which remains an excellent target for new weight-loss therapies, but there remains a gap in knowledge on the mechanisms involved in 5-HT produced in the dorsal Raphe nucleus (DRN) and its involvement in meal initiation. Using a closed-loop optogenetic feeding paradigm, we showed that the 5-HT
DRN →arcuate nucleus (ARH) circuit plays an important role in regulating meal initiation. Incorporating electrophysiology and ChannelRhodopsin-2-Assisted Circuit Mapping, we demonstrated that 5-HTDRN neurons receive inhibitory input partially from GABAergic neurons in the DRN, and the 5-HT response to GABAergic inputs can be enhanced by hunger. Additionally, deletion of the GABAA receptor subunit in 5-HT neurons inhibits meal initiation with no effect on the satiation process. Finally, we identified the instrumental role of dopaminergic inputs via dopamine receptor D2 in 5-HTDRN neurons in enhancing the response to GABA-induced feeding. Thus, our results indicate that 5-HTDRN neurons are inhibited by synergistic inhibitory actions of GABA and dopamine, which allows for the initiation of a meal., Competing Interests: DECLARATION OF INTERESTS: The authors declare no competing interests.- Published
- 2024
- Full Text
- View/download PDF
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