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32P-labeled ribonucleic acid (RNA) from purified Sindbis virus was examined for the presence of hidden breaks. Viral RNA was treated with acid at pH 2.9 or with formaldehyde and was analyzed on sucrose gradients or by polyacrylamide gel electrophoresis. The sedimentation pattern and mobility on polyacrylamide gels of the 42SRNA was unaffected by heating and quick cooling and was not altered by denaturing agents such as dimethyl sulfoxide and urea. No evidence that Sindbis RNA is a polyaggregate of fragments was obtained. It is concluded that the genome consists of a continuous length of single-stranded polynucleotide.

AbstractA previously undescribed entomopoxvirus was isolated from larvae of Choristoneura conflictanaWlk. Samples of larvae taken from several points in Ontario indicated that the virus was widespread in the Province.The morphology of the virus is described. The inclusion bodies are larger than those of another entomopoxvirus originally isolated from Choristoneura biennisFree. and propogated in Choristoneura fumiferana(Clem.). The virions are of the same order of size for both viruses. Microspindles are associated with these viruses and some of the spindles are occluded along with the virions within the oval inclusion bodies. The C. conflictanaentomopoxvirus is also infectious to C. fumiferana.