1. TYROSINASE ISOLATED FROM MOUSE MELANOMA MELANOSOME.
- Author
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Miyazaki, Kazuhiro and Seiji, Makoto
- Subjects
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PHENOL oxidase , *MICE , *MELANOMA , *CHROMATOGRAPHIC analysis , *ELECTROPHORESIS , *POLYACRYLAMIDE , *CELLULOSE acetate - Abstract
Tyrosinase was dissolved from the melanosome fraction of Harding-Passey mouse melanoma and purified further by Sephadex G-100 gel filtration and DEAE-cellulose column chromatography. The specific activity of the purified preparation was 763 times as high as that of the original melanosome. Tyrosinase thus isolated showed only a single activity in both the electrophoresis of a polyacrylamide gel and a cellulose acetate. The Km value is the same as those of the original melanosome and the smooth-surfaced-membrane. The relationship between particle bound tyrosinase and solubilized tyrosinase is discussed in this paper. [ABSTRACT FROM AUTHOR]
- Published
- 1971
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