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TYROSINASE ISOLATED FROM MOUSE MELANOMA MELANOSOME.
- Source :
-
Journal of Investigative Dermatology . Aug71, Vol. 57 Issue 2, p81-86. 6p. - Publication Year :
- 1971
-
Abstract
- Tyrosinase was dissolved from the melanosome fraction of Harding-Passey mouse melanoma and purified further by Sephadex G-100 gel filtration and DEAE-cellulose column chromatography. The specific activity of the purified preparation was 763 times as high as that of the original melanosome. Tyrosinase thus isolated showed only a single activity in both the electrophoresis of a polyacrylamide gel and a cellulose acetate. The Km value is the same as those of the original melanosome and the smooth-surfaced-membrane. The relationship between particle bound tyrosinase and solubilized tyrosinase is discussed in this paper. [ABSTRACT FROM AUTHOR]
Details
- Language :
- English
- ISSN :
- 0022202X
- Volume :
- 57
- Issue :
- 2
- Database :
- Academic Search Index
- Journal :
- Journal of Investigative Dermatology
- Publication Type :
- Academic Journal
- Accession number :
- 12349583
- Full Text :
- https://doi.org/10.1111/1523-1747.ep12349583