1. Aqueous extract of Chrysobalanus icaco leaves, in lower doses, prevent fat gain in obese high-fat fed mice
- Author
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Anne Karoline de Souza Oliveira, W. De Lucca, Lucindo José Quintans-Júnior, A.M. De Oliveira e Silva, Pollyanna A. S. White, Paulo Cesar de Lima Nogueira, Luana M. Cercato, T.C. Goes, V.S. Batista, Antonia Almeida Araújo, Valéria Regina de Souza Moraes, Bruno dos Santos Lima, Enilton A. Camargo, E.R.A. Oliveira, Márcio R. V. Santos, and F.T. Silva
- Subjects
Blood Glucose ,Male ,0301 basic medicine ,medicine.medical_treatment ,Adipose tissue ,Blood lipids ,Motor Activity ,Pharmacology ,Diet, High-Fat ,Weight Gain ,Chrysobalanaceae ,Lipid peroxidation ,Mice ,03 medical and health sciences ,chemistry.chemical_compound ,Drug Discovery ,medicine ,Animals ,Glucose homeostasis ,Obesity ,Food science ,030109 nutrition & dietetics ,Plant Extracts ,Insulin ,Body Weight ,Free Radical Scavengers ,Lipid Metabolism ,Mice, Inbred C57BL ,Plant Leaves ,030104 developmental biology ,chemistry ,Lean body mass ,Anti-Obesity Agents ,medicine.symptom ,Myricitrin ,Weight gain - Abstract
Ethnopharmacological relevance Due to the rise in obesity, the necessity for resources and treatments that could reduce the morbidity and mortality associated to this pandemia has emerged. The development of new anti-obesity drugs through herbal sources has been increasing in the past decades which are being used not only as medicine but also as food supplements. Previous studies with the aqueous extract of Chrysobalanus icaco L (AECI) have demonstrated activity on lowering blood glucose levels and body weight. Aim of the study Investigate C. icaco effects in overall adiposity and glycemic homeostasis. Material and methods C57BL/6J mice were randomly assigned to standard chow (SC) or high-fat diet (HFD) and treated with AECI in 0.35 mg/mL or 0.7 mg/mL concentrations ad libitum. Food intake, feed efficiency, metabolic efficiency, body, fat pads and gastrocnemius weight, adiposity index, serum lipids, fecal lipid excretion, locomotor activity in the open field test and insulin and glucose tolerance tests were analyzed and compared. The major components of the extract were demonstrated through HPLC and its antioxidant activity analyzed through DPPH and lipid peroxidation. Results The AECI in the 0.35 mg/mL concentration did not affect food intake or body weight. However, it promoted lower adipose tissue gain, TG levels, and fecal lipid excretion, increased locomotor activity and lean mass weight, and normalized insulin sensitivity and glucose tolerance. Moreover, AECI showed the presence of myricetin 3-O-glucuronide, rutin, quercitrin and myricitrin and demonstrated high-antioxidant activity. Conclusions AECI in lower concentrations can prevent fat storage or enhance fat utilization through the increase of locomotor activity. Also, this reinforces its ability to maintain glucose homeostasis through the normalization of insulin sensitivity and glucose tolerance despite the high-fat diet intake. These activities could be associated to the extract's polyphenol content.
- Published
- 2016