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Inhibitory effect of O-propargyllawsone in A549 lung adenocarcinoma cells.

Authors :: Dos Santos EWP
de Sousa RC
de Franca MNF
Santos JF
Ottoni FM
Isidório RG
de Lucca Junior W
Alves RJ
Scher R
Corrêa CB
Source :: BMC complementary medicine and therapies [BMC Complement Med Ther] 2023 Sep 20; Vol. 23 (1), pp. 333. Date of Electronic Publication: 2023 Sep 20.
Publication Year :: 2023
Abstract: Background: Lung cancer is the deadliest type of cancer in the world and the search for compounds that can treat this disease is highly important. Lawsone (2-hydroxy-1,4-naphtoquinone) is a naphthoquinone found in plants from the Lawsone genus that show a high cytotoxic effect in cancer cell lines and its derivatives show an even higher cytotoxic effect.<br />Methods: Sulforhodamine B was used to evaluate the cytotoxic activity of compounds on tumor cells. Clonogenic assay was used to analyze the reduction of colonies and wound healing assay to the migratory capacity of A549 cells. Apoptosis and necrosis were analyzed by flow cytometer and Giemsa staining. Hemolysis assay to determine toxicity in human erythrocytes.<br />Results: Lawsone derivatives were evaluated and compound 1 (O-propargyllawsone) was the one with the highest cytotoxic effect, with IC <subscript>50</subscript> below 2.5 µM in A549 cells. The compound was able to reduce colony formation and inhibit cell migration. Morphological changes and cytometry analysis show that the compound induces apoptosis and necrosis in A549 cells.<br />Conclusions: These results show that O-propargyllawsone show a cytotoxic effect and may induce apoptosis in A549 cells.<br /> (© 2023. BioMed Central Ltd., part of Springer Nature.)