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Einen Umweg nehmen: Für die Synthese von Isovaleryl ‐ Coenzym A (CoA) in Myxobakterien wurde kürzlich ein alternativer Stoffwechselweg vorgeschlagen, der unter Leucin ‐ Mangel hochaktiv ist. Die enzymatischen Schritte dieser zuvor unbekannten Biosyntheseroute werden charakterisiert, einschließlich der Identifizierung einer bislang unbekannten 3 ‐ Methylglutaconyl ‐ CoA ‐ Decarboxylase, die wahrscheinlich aus einer CoA ‐ Transferase evolvierte. [ABSTRACT FROM AUTHOR]

Take a detour: An alternative pathway to synthesize isovaleryl coenzyme A (CoA) has recently been suggested in myxobacteria, which is highly active when leucine is limited. Each enzymatic step of this unprecedented route has now been characterized and a novel 3‐methylglutaconyl CoA decarboxylase identified that has apparently evolved from CoA transferases. [ABSTRACT FROM AUTHOR]

Isovaleryl coenzyme A (IV-CoA) is an important precursor for iso-fatty acids and lipids. It acts in the development of myxobacteria, which can produce this compound from acetyl-CoA through alternative IV-CoA biosynthesis (aib). A central reaction of aib is catalyzed by AibA/AibB, which acts as a cofactor-free decarboxylase despite belonging to the family of CoA-transferases. We developed an efficient expression system for AibA/AibB that allowed the determination of high-resolution crystal structures in complex with different ligands. Through mutational studies, we show that an active-site cysteine previously proposed to be involved in decarboxylation is not required for activity. Instead, AibA/AibB seems to induce an intramolecular decarboxylation by binding its substrate in a hydrophobic cavity and forcing it into a bent conformation. Our study opens opportunities for synthetic biology studies, since AibA/AibB may be suitable for the production of isobutene, a precursor of biofuels and chemicals., (© 2017 Wiley-VCH Verlag GmbH & Co. KGaA, Weinheim.)

Ripostatin is a promising antibiotic that inhibits RNA polymerase by binding to a novel binding site. In this study, the characterization of the biosynthetic gene cluster of ripostatin, which is a peculiar polyketide synthase (PKS) hybrid cluster encoding cis- and trans-acyltransferase PKS genes, is reported. Moreover, an unprecedented mechanism for phenyl acetic acid formation and loading as a starter unit was discovered. This phenyl-C2 unit is derived from phenylpyruvate (phenyl-C3) and the mechanism described herein explains the mysterious loss of one carbon atom in ripostatin biosynthesis from the phenyl-C3 precursor. Through in vitro reconstitution of the whole loading process, a pyruvate dehydrogenase like protein complex was revealed that performs thiamine pyrophosphate dependent decarboxylation of phenylpyruvate to form a phenylacetyl-S-acyl carrier protein species, which is supplied to the subsequent biosynthetic assembly line for chain extension to finally yield ripostatin., (© 2017 Wiley-VCH Verlag GmbH & Co. KGaA, Weinheim.)