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By-products from different sources vary widely in their composition and their functional properties. The aim of this study was to produce industrial by-product flours from orange (OF), soybean (SF) and peach palm (PF), besides formulating and characterizing blends with the use of a simplex-centroid mixture design. The formulation containing 100% PF had the highest ash content (67.9 g.kg-1), while the highest lipid (155.8 g.kg-1) and protein (268.2 g.kg-1) contents were found in the formulation that had 100% SF. Total dietary fiber content of the blends produced ranged from 577.7 to 728.5 g.kg-1. The pH and the water activity varied from 4.1 to 6.7 and from 0.484 to 0.611, respectively, indicating stability and safety for consumption of the formulations. The highest swelling volume (20.8 mL.g-1), water and oil absorption (7.4 and 3.6 g.g-1, respectively) was found for 100% PF, which is important to the industrial applications. The simultaneous optimization showed that 100% OF is the most interesting formulation due to the best balance of insoluble to soluble fiber (2:1) and the highest total phenolic content (4.5 mg gallic acid.g-1) and antioxidant activity. However, the binary and ternary mixtures of by-product flours also had interesting functional properties, and they can be used for the development of healthier foods. [ABSTRACT FROM AUTHOR]

Grape juice and wines (Vitis sp.) are the products that are most produced and consumed from different varieties of grapes. About 20% of pomace is generated from processing but there are very few products available with added grape pomace. Consequently, the aim of this study was to characterize flours made from grape skin (GSF) and grape pomace (GPF: seeds + skin) from organic Vitis labrusca L. cv. Bordeaux with respect to physicochemical composition, bioactive compounds content and antioxidant/reducing capacities. The by-products presented a high content of insoluble fibers (55.84 ± 0.63 and 51.02 ± 1.12 g/100 g, respectively); total lipids contents below 10 g/100 g; and >1.900 mg gallic acid equivalent (GAE)/100 g total phenolic content, in which total ortho-diphenols, flavonols, anthocyanins and water-soluble condensed tannins were the main chemical compounds. Regarding the in vitro functionality, which was measured by chemical methods, the GPF and GSF presented values of 1373.64 ± 72.94 and 361.48 ± 2.99 mg GAE/100 g iron reducing capacity; 1574.26 ± 8.94 and 1499.79 ± 31.39 mg ascorbic acid equivalent/100 g in the reduction of DPPH radical; and 2892.46 ± 61.69 and 426.34 ± 18.85 mg of quercetin equivalent/100 g of total reducing capacity, respectively. The results indicate that the GPF and GSF produced from the organic grape juice industry are promising materials for use by food companies. [ABSTRACT FROM AUTHOR]

Thermalgravimetric analysis and differential thermal analysis were employed to study the thermal behavior of gallic acid using regression models. Data were subjected to ordinary least square regression models and results showed that thermal decomposition occurred in three main steps with two endothermic peaks and two exothermic peaks due to oxidation process. The dehydration started at 74°C in a single step until 107°C. After that, the anhydrous compound was stable until 210°C, when the decomposition of organic matter occurred in two consecutive steps. Regression models based on a first-order kinetic of gallic acid decomposition were proposed and equations were deemed statistically significant (p<0.05) and explained suitably the phenomenon. Differential scanning calorimetry (DSC) allowed the determination of the enthalpy of the main events and the Flynn-Wall-Ozawa non-isothermal method was used to investigate the activation energy of decomposition process that was found to be 150.31 kJ.mol-1. [ABSTRACT FROM AUTHOR]

A complete life cycle analysis (LCA) of a refurbished high-end smartphone in Chile is conducted to find out its environmental impacts and evaluate potential advantages compared to a new one and a modular alternative. The functional unit is defined as the daily use of smartphone for 3 years, including audio, video, and internet. The results show that use and production phase are the most impactful (52,0% and 45,2%, respectively), but phases contribute differently depending on the impact category. It is also found that a refurbished smartphone presents 71% and 60% lower global warming potential than a new version and modular smartphone, respectively. The lower impacts of the refurbished alternative are due to the reutilization of the device avoiding the impacts related to material extraction and device production. However, it is not possible to assess the significance of these differences due to the lack of uncertainty analysis in other studies. In conclusion, refurbishing can avoid extraction and production phases which reduces environmental impacts. Yet, to appropriately compare different circular models, it is imperative to calculate and publish the uncertainty of LCA results.

This study identifies key challenges to measure the carbon footprint (CF) over the whole life cycle of buildings in Chile, although its findings are also applicable to other countries. This paper presents four of the said challenges that emerge from the analysis of certification programs, design guides, databases, CF/LCA calculators, standards, and Measurement, Reporting and Verification (MRV) programs worldwide. The four challenges are: (i) a sustainable governance for the carbon footprint system which ensures CF quality, enables regular updates of the data, and has guaranteed funding, (ii) adoption of the system by industry, (iii) standardization of reporting, and (iv) standardization of measurements over the full life cycle. An MRV appears as the best option to coordinate multiple actors and data needs; however, it requires a sustainable governance model as well as widespread adoption. Such an MRV would need a common reporting system. However, comparability may be compromised due to a lack of consensus on which standard to follow in the industry and on definitions for basic building measurements, such as area. The reporting system must be based on a standardized tool, i.e., a CF calculator for buildings. Many of these exist; however, they differ in a fundamental aspect: whether they are used to guide low carbon design or to certify carbon emissions by buildings. Finally, the calculator will need an agreed upon methodology. Although several standards exist, one must be chosen, updated and deployed. Some countries may be more advanced than others on these challenges, but none have solved them completely. A concerted effort would be best to reduce the CF of the construction sector globally.

This study aimed to utilize blueberry pectin and calcium chloride to design a gel network structure for loading nut oils (peanut and walnut oil, respectively). The optimization of emulsion gel preparation was conducted through orthogonal experiments, utilizing the oil-holding ratio and gel strength as critical indicators. The emulsion gel was applied to the ice cream production. It was revealed that the peroxide value of the nut oil emulsion gels was significantly lower than that of nut oils. Both nut oil emulsion gel ice creams exhibited higher expansion rates, lower melting rates, and decreased hardness than the nut oil ice creams. Notably, walnut oil emulsion gel ice cream demonstrated a melting rate similar to traditional butter-based ice cream. Emulsion gel ice cream has higher fat globule instability and viscosity. Overall, the comprehensive emulsion gel ice cream indicators were comparable to conventional butter ice cream and notably superior to peanut and walnut oil ice cream. Using emulsion gel as a fat substitute in ice cream was feasible. The implications of these results were significant for advancing the utilization of nut oil emulsion gel within the ice cream industry., Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2024 The Authors. Published by Elsevier B.V. All rights reserved.)

Galactoglucomannan (GGM) is the predominant hemicellulose in coniferous trees, such as Norway spruce, and has been used as a multipurpose emulsifier in the food industry. In vitro digestion with a cellular antioxidant activity assay was performed to determine the bioaccessibility and antioxidant activity of phenolic compounds, and the behaviour of GGM on in vivo experimental assay against induced colon cancer. The results showed that digestion decreased the bioaccessibility and antioxidant capacity of phenolic compounds. Cellular analysis did not support these findings once an antioxidant effect was observed in human cell lines. GGM attenuated the initiation and progression of colon cancer, by reducing the foci of aberrant crypts in rats, and modified the intestinal bacterial microbiota (disrupting the balance between Firmicutes and Bacteroidetes phyla). Thus, GGM provided chemopreventive protection against the development of colon cancer and acted as an intracellular antioxidant agent., Competing Interests: Declaration of competing interest The authors declare the following financial interests/personal relationships which may be considered as potential competing interests: Luciana Azevedo reports financial support was provided by Minas Gerais State Foundation of Support to the Research. Luciana Azevedo reports financial support was provided by National Council for Scientific and Technological Development., (Copyright © 2024 The Authors. Published by Elsevier B.V. All rights reserved.)

Background: Type 2 diabetes mellitus (T2DM) is a chronic metabolic disorder characterized by elevated blood glucose levels, posing a significant global health concern due to its increasing prevalence. Insulin resistance (IR) plays a major role in the development of T2DM and is often linked to factors such as obesity, physical inactivity, and a sedentary lifestyle. Recently, there has been growing interest in exploring the potential of natural products for improving insulin sensitivity and glucose metabolism. Among these, Cynomorium songaricum Rupr., an edible parasitic plant, has shown promising antidiabetic effects. However, research on its beneficial effects on IR is still nascent. Therefore, this study aims to investigate the application of a Cynomorium songaricum flavonoid-enriched fraction (CSF) in the treatment of IR in T2DM, along with elucidating the chemical and biochemical mechanisms involved., Method: First, UHPLC/ESI-LTQ-Orbitrap-MS was utilized to perform a chemical profiling of CSF. Subsequently, glycogen synthesis, gluconeogenesis and glucose consumption assays were conducted on HepG2 cells with a high glucose high insulin-induced IR model to illustrate the favorable impacts of CSF on IR. Then, an innovative network pharmacology analysis was executed to predict the potential chemical components and hub genes contributing to CSF's protective effect against IR. To further elucidate molecular interactions, molecular docking studies were performed, focusing on the binding interactions between active constituents of CSF and crucial targets. Additionally, an RNA-sequencing assay was employed to uncover the underlying biochemical signaling pathway responsible for CSF's beneficial effects. To validate these findings, western blot and qPCR assays were employed to verify the pathways related to IR and the potential signaling cascades leading to the amelioration of IR., Results: The UHPLC/ESI-LTQ-Orbitrap-MS analysis successfully identified a total of thirty-six flavonoids derived from CSF. Moreover, CSF was shown to significantly improve glycogen synthesis and glucose consumption as well as inhibit gluconeogenesis in HepG2 cells of IR. An innovative network pharmacology analysis unveiled key hub genes-AKT1 and PI3K-integral to metabolic syndrome-related signaling pathways, which contributed to the favorable impact of CSF against IR. Noteworthy active ingredients including quercetin, ellagic acid and naringenin were identified as potential contributors to these effects. The results of western blot and qPCR assays provided compelling evidence that CSF improved insulin sensitivity by modulating the PI3K-Akt signaling pathway. Subsequent RNA-sequencing analysis, in tandem with western blot assays, delved deeper into the potential mechanisms underlying CSF's advantageous effects against IR, potentially associated with the enhancement of endoplasmic reticulum (ER) proteostasis., Conclusion: CSF exhibited a remarkable ability to enhance insulin sensitivity in the IR model of HepG2 cells. This was evident through enhancements in glycogen synthesis and glucose consumption, along with its inhibitory impact on gluconeogenesis. Furthermore, CSF demonstrated an improvement in the insulin-mediated PI3K-Akt signaling pathway. The potential active constituents were identified as quercetin, ellagic acid and naringenin. The underlying biochemical mechanisms responsible for CSF's beneficial effects against IR were closely linked to its capacity to mitigate ER stress, thereby offering a comprehensive understanding of its protective action., Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2024. Published by Elsevier GmbH.)

Baltic herring is the main catch in the Baltic Sea; however, its usage could be improved due to the low processing rate. Previously we have shown that whole Baltic herring hydrolysates (BHH) and herring byproducts hydrolysates (BHBH) by commercial enzymes consisted of bioactive peptides and had moderate bioactivity in in vitro dipeptidyl peptidase (DPP)-4 assay. In this study, we identified the hydrolysate peptides by LC-MS/MS and predicted the potential bioactive DPP-4 inhibitory peptides using in silico tools. Based on abundance, peptide length and stability, 86 peptides from BHBH and 80 peptides from BHH were proposed to be novel DPP-4 inhibitory peptides. BHH was fed to a mice intervention of a high-fat, high-fructose diet to validate the bioactivity. The results of the glucose tolerance and insulin tolerance improved. Plasma DPP-4 activities, C-peptide levels, and HOMA-IR scores significantly decreased, while plasma glucagon-like peptide-1 content increased. In conclusion, BHH is an inexpensive and sustainable source of functional antidiabetic ingredients., Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2024 The Author(s). Published by Elsevier Ltd.. All rights reserved.)

Tea is the second largest nonalcoholic beverage in the world due to its characteristic flavor and well-known functional properties in vitro and in vivo. Global tea production reaches 6.397 million tons in 2022 and continues to rise. Fresh tea leaves are mainly harvested in spring, whereas thousands of tons are discarded in summer and autumn. Herein, pruned tea biomass refers to abandon-plucked leaves being pruned in the non-plucking period, especially in summer and autumn. At present, no relevant concluding remarks have been made on this undervalued biomass. This review summarizes the seasonal differences of intrinsic metabolites and pays special attention to the most critical bioactive and flavor compounds, including polyphenols, theanine, and caffeine. Additionally, meaningful and profound methods to transform abandon-plucked fresh tea leaves into high-value products are reviewed. In summer and autumn, tea plants accumulate much more phenols than in spring, especially epigallocatechin gallate (galloyl catechin), anthocyanins (catechin derivatives), and proanthocyanidins (polymerized catechins). Vigorous carbon metabolism induced by high light intensity and temperature in summer and autumn also accumulates carbohydrates, such as soluble sugars and cellulose. The characteristics of abandon-plucked tea leaves make them not ideal raw materials for tea, but suitable for novel tea products like beverages and food ingredients using traditional or hybrid technologies such as enzymatic transformation, microbial fermentation, formula screening, and extraction, with the abundant polyphenols in summer and autumn tea serving as prominent flavor and bioactive contributors., (© 2024 Institute of Food Technologists®.)

Oxidative/nitrosative damage takes part in chronic disease development, which generates an urgent need for intervention and better therapies to manage them. The scientific community has demanded easy-to-run, cheap, and reliable methods for cellular antioxidant activity assays. This work standardised and validated an erythrocyte cellular antioxidant activity and membrane protection/injury (HERYCA-P) protocol to study food-derive extracts. The method measures intracellular reactive oxygen species (ROS) generation, lipoperoxidation, and haemolysis induced by 2,2'-azobis(2-amidinopropane) dihydrochloride. Quercetin decreased ROS generation by 50.4% and haemolysis by 2.2%, while ascorbic acid inhibited lipid peroxidation by 40.1%. Total phenolic contents of teas were correlated with decreased ROS generation (r = -0.924), lipoperoxidation (r = -0.951), and haemolysis (r = -0.869). The erythrocyte ROS generation and lipoperoxidation were also associated with CUPRAC (r = -0.925; r = -0.951) and hydroxyl radical scavenging activity (r = -0.936; r = -0.949). The precision rates of antioxidant standards and tea samples were below 15%. HERYCA-P is feasible as a complementary antioxidant assay for food matrices., Competing Interests: Declaration of Competing Interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2023 Elsevier Ltd. All rights reserved.)