1. Physicochemical characterization and immunological properties of Pichia pastoris based HPV16L1 and 18L1 virus like particles.
- Author
-
Gupta G, Glueck R, and Rishi N
- Subjects
- Animals, Antibodies, Viral immunology, Blotting, Western, Capsid Proteins genetics, Capsid Proteins metabolism, Chemical Phenomena, Enzyme-Linked Immunosorbent Assay, Female, Glycosylation, Immunoglobulin G immunology, Mice, Inbred BALB C, Oncogene Proteins, Viral genetics, Oncogene Proteins, Viral metabolism, Papillomavirus Infections immunology, Papillomavirus Infections virology, Papillomavirus Vaccines chemistry, Papillomavirus Vaccines genetics, Pichia genetics, Recombinant Proteins chemistry, Recombinant Proteins metabolism, Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization, Vaccination, Virion genetics, Virion metabolism, Capsid Proteins immunology, Oncogene Proteins, Viral immunology, Papillomavirus Vaccines immunology, Recombinant Proteins immunology, Virion immunology
- Abstract
There continues to be an urgent need for cost-effective prophylaxis for HPV-associated cancers in socio-economically underdeveloped nations. Presently HPV vaccines, which are commercially available, are adjuvanted virus-like particles (VLPs) expressed from various recombinant expression systems. They have been characterized by different methods as safe, pure, and potent HPV vaccine antigens. We cloned and expressed L1 proteins of HPV16 & 18 in Pichia pastoris and tested their immunogenicity. We observed that HPVL1 proteins (16L1 and 18L1) are expressed in Pichia pastoris at high levels. Critical physicochemical parameters of these HPV recombinant L1 proteins were characterized by SDS PAGE, western blotting, peptide mapping, glycosylation pattern, mass spectrometry, host cell DNA and protein analysis, electron microscopy, and immunogenicity analysis. These data establish a blueprint of HPV recombinant protein antigens for standardizing & developing an alternative high-quality, cost-effective vaccine for HPV as well as similar recombinant protein-based vaccines., (Copyright © 2016 International Alliance for Biological Standardization. Published by Elsevier Ltd. All rights reserved.)
- Published
- 2017
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