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Physicochemical characterization and immunological properties of Pichia pastoris based HPV16L1 and 18L1 virus like particles.
- Source :
-
Biologicals : journal of the International Association of Biological Standardization [Biologicals] 2017 Mar; Vol. 46, pp. 11-22. Date of Electronic Publication: 2016 Dec 21. - Publication Year :
- 2017
-
Abstract
- There continues to be an urgent need for cost-effective prophylaxis for HPV-associated cancers in socio-economically underdeveloped nations. Presently HPV vaccines, which are commercially available, are adjuvanted virus-like particles (VLPs) expressed from various recombinant expression systems. They have been characterized by different methods as safe, pure, and potent HPV vaccine antigens. We cloned and expressed L1 proteins of HPV16 & 18 in Pichia pastoris and tested their immunogenicity. We observed that HPVL1 proteins (16L1 and 18L1) are expressed in Pichia pastoris at high levels. Critical physicochemical parameters of these HPV recombinant L1 proteins were characterized by SDS PAGE, western blotting, peptide mapping, glycosylation pattern, mass spectrometry, host cell DNA and protein analysis, electron microscopy, and immunogenicity analysis. These data establish a blueprint of HPV recombinant protein antigens for standardizing & developing an alternative high-quality, cost-effective vaccine for HPV as well as similar recombinant protein-based vaccines.<br /> (Copyright © 2016 International Alliance for Biological Standardization. Published by Elsevier Ltd. All rights reserved.)
- Subjects :
- Animals
Antibodies, Viral immunology
Blotting, Western
Capsid Proteins genetics
Capsid Proteins metabolism
Chemical Phenomena
Enzyme-Linked Immunosorbent Assay
Female
Glycosylation
Immunoglobulin G immunology
Mice, Inbred BALB C
Oncogene Proteins, Viral genetics
Oncogene Proteins, Viral metabolism
Papillomavirus Infections immunology
Papillomavirus Infections virology
Papillomavirus Vaccines chemistry
Papillomavirus Vaccines genetics
Pichia genetics
Recombinant Proteins chemistry
Recombinant Proteins metabolism
Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
Vaccination
Virion genetics
Virion metabolism
Capsid Proteins immunology
Oncogene Proteins, Viral immunology
Papillomavirus Vaccines immunology
Recombinant Proteins immunology
Virion immunology
Subjects
Details
- Language :
- English
- ISSN :
- 1095-8320
- Volume :
- 46
- Database :
- MEDLINE
- Journal :
- Biologicals : journal of the International Association of Biological Standardization
- Publication Type :
- Academic Journal
- Accession number :
- 28012703
- Full Text :
- https://doi.org/10.1016/j.biologicals.2016.12.002