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Hyperlipidemia contributes significantly in the manifestation and development of atherosclerosis and coronary heart disease (CHD). Although synthetic lipid-lowering drugs are useful in treating hyperlipidemia, there are number of adverse effects. So the current interest has stimulated the search for new lipid-lowering agents with minimal side effects from natural sources. The present study was designed to investigate the antihyperlipidemic and antiatherogenic potentiality of ethanolic extract of Terminalia pallida fruits in high fat diet-induced hyperlipidemic rats. T. pallida fruits ethanolic extract (TPEt) was prepared using Soxhlet apparatus. Sprague-Dawley male rats were made hyperlipidemic by giving high fat diet, supplied by NIN (National Institute of Nutrition), Hyderabad, India. TPEt was administered in a dose of 100 mg/kg.b.w./day for 30 days in high fat diet-induced hyperlipidemic rats. The body weights, plasma lipid, and lipoprotein levels were measured before and after the treatment. TPEt showed significant antihyperlipidemic and antiatherogenic activities as evidenced by significant decrease in plasma total cholesterol, triglycerides, low-density lipoprotein cholesterol, and very low-density lipoprotein cholesterol levels coupled together with elevation of high-density lipoprotein cholesterol levels and diminution of atherogenic index in high fat diet-induced hyperlipidemic rats. There was a significantly reduced body weight gain in TPEt-treated hyperlipidemic rats than in the control group. The present study demonstrates that TPEt possesses significant antihyperlipidemic and antiatherogenic properties, thus suggesting its beneficial effect in the treatment of cardiovascular diseases. [ABSTRACT FROM AUTHOR]

Type 2 diabetes has become one of the major health concerns of the 21st century, marked by hyperglycemia or glycosuria, and is associated with the development of several secondary health complications. Due to the fact that chemically synthesized drugs lead to several inevitable side effects, new antidiabetic medications from plants have gained substantial attention. Thus, the current study aims to evaluate the antidiabetic capacity of the Ageratina adenophora hydroalcoholic (AAHY) extract in streptozotocin–nicotinamide (STZ–NA)- induced diabetic Wistar albino rats. The rats were segregated randomly into five groups with six rats each. Group I was normal control, and the other four groups were STZ–NA-induced. Group II was designated diabetic control, and group III, IV, and V received metformin (150 mg/kg b.w.) and AAHY extract (200 and 400 mg/kg b.w.) for 28 days. Fasting blood glucose, serum biochemicals, liver and kidney antioxidant parameters, and pancreatic histopathology were observed after the experimental design. The study concludes that the AAHY extract has a significant blood glucose lowering capacity on normoglycemic (87.01 ± 0.54 to 57.21 ± 0.31), diabetic (324 ± 2.94 to 93 ± 2.04), and oral glucose-loaded (117.75 ± 3.35 to 92.75 ± 2.09) Wistar albino rats. The in vitro studies show that the AAHY extract has αglucosidase and α-amylase inhibitory activities which can restore the altered blood glucose level, glycated hemoglobin, body weight, and serum enzymes such as serum glutamic pyruvic transaminase, serum glutamic oxaloacetic transaminase, serum alkaline phosphatase, total protein, urea, and creatinine levels close to the normal range in the treated STZ–NA-induced diabetic rats. The evaluation of these serum biochemicals is crucial for monitoring the diabetic condition. The AAHY extract has significantly enhanced tissue antioxidant parameters, such as superoxide dismutase, glutathione, and lipid peroxidation, close to normal levels. The presence of high-quantity chlorogenic (6.47% w/w) and caffeic (3.28% w/w) acids as some of the major phytoconstituents may contribute to the improvement of insulin resistance and oxidative stress. The study provides scientific support for the utilization of A. adenophora to treat type 2 diabetes in the STZ–NA-induced diabetic rat model. Although the preventive role of the AAHY extract in treating Wistar albino rat models against type 2 diabetes mellitus is undeniable, further elaborative research is required for efficacy and safety assessment in human beings. [ABSTRACT FROM AUTHOR]

The study was designed to evaluate the geraniol's effect on the kidney and liver damage triggered by a high‐fat diet (HFD)/streptozotocin (STZ)‐induced type 2 diabetes mellitus in rats. Firstly, in vitro inhibitory geraniol's effect against α‐amylase and α‐glucosidase activities was confirmed by in‐silico molecular docking, which revealed higher binding affinity toward α‐amylase than for α‐glucosidase. The protective effect of geraniol with two separate doses (100 and 200 mg/kg b.wt) against diabetes' kidney and liver dysfunctions in Wistar rats was then evaluated. Results indicate that geraniol treatment for 6 weeks instigated a remarkable retrieval of the lipid profile, liver, and kidney function. Geraniol noticeably reinforced the antioxidant enzymes, parameters of oxidative stress of the kidney and liver in this type of diabetes. Also, geraniol treatment prompted a significant reduction in the expression levels of caspase‐3 (Casp3) and Bax mRNA. In conclusion, geraniol ameliorates the progress of HFD/STZ‐induced type 2 diabetes mellitus in rats via dependently upregulating the expression of Bcl‐2 and downregulating Casp3 and Bax expression. Practical applications: Geraniol is a naturally occurring substantial acyclic monoterpenoid compound that has numerous pharmacological activities. The positive effect of geraniol in inhibiting in vitro diabetic markers by a silico molecular docking study was evidenced by illustrating the binding sites of geraniol with both α‐amylase and α‐glucosidase. This study also focused on the therapeutic impact of geraniol against the damage caused by high fat‐diet/streptozotocin‐induced type 2 diabetic complications in Wistar rats. Geraniol limits the oxidative stress of liver and kidney tissue, and it might be used as an antiapoptotic agent in type 2 diabetes. [ABSTRACT FROM AUTHOR]

The present study reports that garlic (Allium sativum) has the potentiality to reduce sodium selenite and synthesize selenium nanoparticles. The exposure of aqueous sodium selenite to the Allium sativum abstract leads to the synthesis of stable selenium nanoparticles within seven days. The colorless appearance of the reaction medium was turned to red color indicates the process of reduction. The mechanism of reduction was analyzed through UV-Visible (UV-Vis) Spectrophotometer, the spectrum showed major peak at 29 nm. Fourier Transform Infra-Red Spectroscopic (FT-IR) analysis substantiated the existence of bonds for the organic contents of garlic extract and to find out the crystallite size and miller indices; x ray diffraction (XRD) was also mentioned in this paper. This biogenic synthesis is a cost effective, simple and ecofriendly method. Kidney injury is a clinical syndrome associated with the incidence of rhabdomyolysis. The current study was carried out to evaluate whether selenium nanoparticles can protect against the alloxan-induced kidney injury. Rats were randomly divided into six groups (n=7) and then the experimental processes conducted for thirty days, the results proved that SeNPs reduced the biochemical and histological changes produced by alloxan. And in general, they could be used to protect against kidney disorder development via antioxidant, anti-inflammatory and anti-apoptotic activities. [ABSTRACT FROM AUTHOR]

Autoimmune destruction of insulin producing pancreatic β-cells leads to insulin insufficiency and hyperglycemia in type 1 diabetes mellitus. Regeneration of β-cells is one of the proposed treatment for type 1 diabetes and insulin insufficiency. Picrorhiza kurroa is a medicinal herb and is traditionally being used for the treatment of various diseases. Previous studies reported the hypoglycemic potential of P. kurroa. However, its potential role in β-cell induction in insulin secretion have not been fully investigated. Here, we characterized the hydro alcoholic extract of P. kurroa rhizome (PKRE) and further studied its β-cell regeneration and induction of insulin secretion potential in streptozotocin (STZ) induced diabetic rats as well as in insulin producing Rin5f cells. 1H-NMR revealed the presence of more than thirty metabolites including picroside I and II in PKRE. Further, we found that PKRE treatment (100 and 200 mg/kg dose for 30 days) significantly (p ≥ 0.05) protected the pancreatic β-cells against streptozotocin (STZ) evoked damage and inhibited the glucagon receptor expression (Gcgr) in hepatic and renal tissues. It significantly (p ≥ 0.05) enhanced the insulin expression and aids in proliferation of insulin producing Rin5f cells with elevated insulin secretion. Furthermore it significantly (p ≥ 0.05) increased insulin mediated glucose uptake in 3T3L1 and L6 cells. On the contrary, in diabetic rats, PKRE significantly (p ≥ 0.05) decreased high blood glucose and restored the normal levels of serum biochemicals. Altogether, our results showed that PKRE displayed b-cell regeneration with enhanced insulin production and antihyperglycemic effects. PKRE also improves hepatic and renal functions against oxidative damage. [ABSTRACT FROM AUTHOR]

Elderberry (Sambucus nigra L.) lipophilic and polar extract dietary supplementation effects were evaluated according to diabetes management indices, using an in vivo model. A research pipeline was constructed, that ranged from extract preparation, partial chemical characterization and toxicity evaluation, to examining the elderberry extract dietary supplementation effects on biofluid and tissues. Extracts toxicity was screened using an Aliivibrio fischeri bioluminescence model. A concentration of up to 60 mg/L was selected, and rat doses for oral supplementation were computed applying the interspecies correlation between A. fischeri and rats. Wistar type 2 diabetic rats, induced by streptozotocin (STZ), were fed a high-fat diet and supplemented for 4 weeks at doses of 190 and 350 mg/kg body weight/day of lipophilic and polar extract, respectively. As far as we know, lipophilic elderberry extract supplementation was assessed for the first time, while polar extract was administrated at higher doses and for a shorter period compared to previous studies, aiming to evaluate subacute supplementation effects. The polar extract modulated glucose metabolism by correcting hyperglycemia, while the lipophilic extract lowered insulin secretion. Both extracts lowered insulin resistance, without remarkable alterations to hematological indices, sera lipids and sera and tissular trace element homeostasis. In conclusion, elderberries are a potential source of bioactive compounds for formulations to be used as co-adjuvants in diabetes management. [ABSTRACT FROM AUTHOR]