1. Reactive aldehyde-scavenging enzyme activities in atherosclerotic plaques of cigarette smokers and nonsmokers.
- Author
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Lapenna D, Ciofani G, Ucchino S, Giamberardino MA, Di Ilio C, and Cuccurullo F
- Subjects
- Aged, Aldehyde Dehydrogenase 1 Family, Aldehyde Dehydrogenase, Mitochondrial, Biomarkers analysis, Carotid Arteries pathology, Carotid Arteries surgery, Carotid Artery Diseases diagnosis, Carotid Artery Diseases etiology, Carotid Artery Diseases surgery, Down-Regulation, Female, Humans, Isoenzymes analysis, Lipid Peroxidation, Male, Middle Aged, Oxidative Stress, Prognosis, Retinal Dehydrogenase analysis, Severity of Illness Index, Aldehyde Dehydrogenase analysis, Aldehyde Reductase analysis, Carotid Arteries enzymology, Carotid Artery Diseases enzymology, Glutathione Transferase analysis, Plaque, Atherosclerotic, Smoking adverse effects
- Abstract
Objective: To investigate enzymatic reactive aldehyde-scavenging enzyme capacity together with lipid peroxidation as expression of oxidative stress in atherosclerotic plaques of cigarette smokers and nonsmokers., Methods: We have assessed specific enzymatic activities of class 1, 2, and 3 aldehyde dehydrogenase (ALDH1, ALDH2, and ALDH3, respectively), glutathione S-transferase (isozyme A4-4, GSTA4-4), and aldose reductase (AR), namely the major reactive aldehyde-scavenging enzymes, together with lipid peroxidation, i.e., fluorescent damage products of lipid peroxidation (FDPL), in carotid atherosclerotic plaques surgically removed from 17 cigarette smokers and 17 nonsmokers., Results: The enzymatic activities of ALDH1 plus ALDH2, ALDH3, GSTA4-4, and AR were significantly lower in the atherosclerotic plaques of smokers than in those of nonsmokers, while plaque FDPL levels were significantly higher in the smokers than in the nonsmokers. The amount of cigarette smoking was correlated inversely with the aforementioned plaque enzymatic activities and directly with plaque FDPL content. Plaque FDPL levels were inversely correlated with plaque enzymatic activities in smokers and nonsmokers. The degree of carotid atherosclerotic stenosis, as expression of atherosclerosis severity, was correlated inversely with plaque enzymatic activities and directly with plaque FDPL levels in smokers and nonsmokers; moreover, the degree of carotid stenosis was directly correlated with the amount of cigarette smoking., Conclusion: atherosclerotic lesions of cigarette smokers are endowed with a depressed enzymatic reactive aldehyde-scavenging capacity eventually favoring oxidative stress and the severity of atherosclerosis., (Copyright © 2014 Elsevier Ireland Ltd. All rights reserved.)
- Published
- 2015
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