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Proteome analysis of X-ray irradiated human erythroleukemia cells
- Source :
-
BBA - Proteins & Proteomics . Apr2008, Vol. 1784 Issue 4, p611-620. 10p. - Publication Year :
- 2008
-
Abstract
- Abstract: In order to discover molecular biomarkers in radiation response we investigated the effects of X-radiation on radioresistant K562 cells by using a comparative proteomic analysis. In treated cells 29 up-regulated and 10 down-regulated proteins were detected by image analysis and identified by mass spectrometry. Elongation factor 1 alpha 1 and stress-70 protein showed a 6.2 and 5.4 fold increase respectively in treated cells. Additional proteins such us pi and omega classes glutathione transferases, ATP synthase D chain, were also found to be up-regulated, suggesting that the enzyme belonging to the cellular detoxification system against oxidative stress and energetic metabolism may have a key role in the cellular response to radiation injury. This data set may provide a useful tool to design a combined chemo- and radiotherapic strategy against leukemia disease. [Copyright &y& Elsevier]
- Subjects :
- *RADIATION
*MOLECULAR biology
*BIOMARKERS
*MASS spectrometry
*LEUKEMIA
*PREVENTION
Subjects
Details
- Language :
- English
- ISSN :
- 15709639
- Volume :
- 1784
- Issue :
- 4
- Database :
- Academic Search Index
- Journal :
- BBA - Proteins & Proteomics
- Publication Type :
- Academic Journal
- Accession number :
- 31405731
- Full Text :
- https://doi.org/10.1016/j.bbapap.2008.01.018