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Evaluates the impact of three different soil deoxyribonucleic acid (DNA) extraction methods on bacterial diversity using polymerase chain reaction-based 16S ribosomal nucleic acid analysis. Properties of the soil used in DNA extraction method; Revelation of phylotype abundance and bacterial community composition by ribosomal intergenic spacer analysis.

Investigates the diversity and seasonal fluctuations of soil microbial community of a wheat field farm in the Netherlands. Phylogenetic analysis of the dominant 16S ribosomal deoxyribonucleic clone sequences; Population of Arthrobacter oxydans and members of the genus Pseudomonas in July; High number of Acinobacter and Clavibacter in January.

The article presents an inquiry of a microbiologist regarding the origin of species diversity. She mentions about the estimates of the total number of bacteria in the biosphere and the major goal of microbiology. The author also traces the first inkling of the huge diversity of the microbial world, which starts from the use of ribosomal-ribonucleic acid (RNA) typing in the late 1980s. Furthermore, she cites several studies that examine the microbial content of soils, sediments and hypersaline environments.