50 results on '"Xiao-Lin Li"'
Search Results
2. Should acupuncture therapy be used for acute facial paralysis? A protocol for systematic review
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Lu Cheng, Xiao-lin Li, Yi Ying, Shi-hao Du, Xu-dong Zhang, Wei Guo, Shu-qi Mi, and Ji-ping Zhao
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Acupuncture therapy ,Peripheral facial paralysis ,Acute phase ,Systematic review ,Protocol ,Medicine - Abstract
Abstract Background Peripheral facial paralysis (PFP) results in functional disorder and social dysfunction, when it is under a severe condition at onset, long-term poor outcomes do occur. Different acupuncture methods have been reported to be potentially effective for shortening the disease course and reducing the occurrence of sequelae when they are applied at an early stage. Neuro edema is a common pathological feature in the acute phase, and many clinical studies have suggested its effect of reducing facial nerve edema. It is of value to estimate the effectiveness and safety of acupuncture treatment at the onset, and to assess the most suitable acupuncture method for the acute period. Methods and analysis All the RCTs and quasi-RCTs on acupuncture therapy for patients who is during acute stage of PFP will be included. The recovery rate of facial function, the time it takes to restore facial function and the odds of sequelae occurring will be the key parts we focus on. Psychological well-being and quality of life will also be evaluated. Literature searching will be conducted until December 31th, 2022 from eight databases systematically. Two reviewers will screen the literature and extract the data independently. RevMan software will be used for data analysis, and the version 2 of the Cochrane risk-of-bias tool (RoB 2) will be used to assess the certainty of evidence. Forest plots and summary findings will be generated. If data permits, a meta-analysis will be conducted. Ethics and dissemination Since this study will not involve clinical treatment of patients, ethics approval is not required. The result of this study will be submitted to a peer-reviewed journal for publication and as a proposal for clinical practice and further study on acupuncture treatment at the early stage of PFP. Discussion This review will summarize the evidence on the different type of acupuncture therapy for acute Bell’s palsy and Ramsay-Hunt syndrome. We anticipate that it would be safe and effective when applied to the acute phase of PFP, and some specific suitable acupuncture methods would be found resulting from this review. Systematic review registration International Prospective Register for Systematic Reviews (PROSPERO) number CRD42020205127
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- 2023
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3. Insights into lipid metabolism and immune-inflammatory responses in the pathogenesis of coronary artery ectasia
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Li Jiang, Wei Wei, Sheng Kang, Xiao-Lin Li, and Yu Luo
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coronary artery ectasia ,lipid metabolism ,cytokine ,immune-inflammatory response ,pathogenesis ,Physiology ,QP1-981 - Abstract
Coronary artery ectasia (CAE) is a rare finding that is associated with poor clinical outcomes (Kawsara et al. 2018), and disorders in lipid metabolism have been reported in CAE. Lipids constitute one of the three metabolite types that regulate bodily functions and are also powerful signaling molecules (Han 2016; Zhu et al. 2021) that affect immunoregulation and inflammatory responses via a series of transcription factors and signaling pathways (Barrera et al. 2013). Although abnormal lipid metabolism and immunoinflammatory responses have been reported in CAE, their roles in the pathogenic mechanisms underlying CAE are currently unclear.
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- 2023
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4. Prevention and control of HIV/AIDS in China: lessons from the past three decades
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Jun-Jie Xu, Meng-Jie Han, Yong-Jun Jiang, Hai-Bo Ding, Xi Li, Xiao-Xu Han, Fan Lv, Qing-Feng Chen, Zi-Ning Zhang, Hua-Lu Cui, Wen-Qing Geng, Jing Zhang, Qi Wang, Jing Kang, Xiao-Lin Li, Hong Sun, Ya-Jing Fu, Ming-Hui An, Qing-Hai Hu, Zhen-Xing Chu, Ying-Jie Liu, Hong Shang, and Peng Lyu
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Medicine - Abstract
Abstract. In the past 37 years, human immunodeficiency virus/acquired immunodeficiency syndrome (HIV/AIDS) has undergone various major transmission routes in China, with the world most complex co-circulating HIV-1 subtypes, even the prevalence is still low. In response to the first epidemic outbreak of HIV in injecting drug users and the second one by illegal commercial blood collection, China issued the Anti-Drug Law and launched the Blood Donation Act and nationwide nucleic acid testing, which has avoided 98,232 to 211,200 estimated infections and almost ended the blood product-related infection. China has been providing free antiretroviral therapy (ART) since 2003, which covered >80% of the identified patients and achieved a viral suppression rate of 91%. To bend the curve of increasing the disease burden of HIV and finally end the epidemic, China should consider constraining HIV spread through sexual transmission, narrowing the gaps in identifying HIV cases, and the long-term effectiveness and safety of ART in the future.
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- 2021
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5. Pharmacokinetics of immediate and sustained-release formulations of paroxetine: Population pharmacokinetic approach to guide paroxetine personalized therapy in chinese psychotic patients
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Xiao-lin Li, Shan-qing Huang, Tao Xiao, Xi-pei Wang, Wan Kong, Shu-jing Liu, Zi Zhang, Ye Yang, Shan-shan Huang, Xiao-jia Ni, Hao-yang Lu, Ming Zhang, Yu-guan Wen, and De-wei Shang
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paroxetine ,Chinese ,dose-dependent ,formulation ,population pharmacokinetics ,Therapeutics. Pharmacology ,RM1-950 - Abstract
Paroxetine is one of the most potent selective serotonin reuptake inhibitors (SSRIs) approved for treating depression, panic disorder, and obsessive-compulsive disorder. There is evidence linking genetic polymorphisms and nonlinear metabolism to the Paroxetine’s pharmacokinetic (PK) variability. The purpose of the present study was to develop a population PK (PPK) model of paroxetine in Chinese patients, which was used to define the paroxetine’s PK parameters and quantify the effect of clinical and baseline demographic factors on these PK characteristics. The study included 184 inpatients with psychosis (103 females and 81 males), with a total of 372 serum concentrations of paroxetine for PPK analyses. The total daily dosage ranged from 20 to 75 mg. One compartment model could fit the PKs characterize of paroxetine. Covariate analysis revealed that dose, formulation, and sex had a significant effect on the PK parameters of paroxetine; however, there was no evident genetic influence of CYP2D6 enzymes on paroxetine concentrations in Chinese patients. The study determined that the population’s apparent distribution volume (V/F) and apparent clearance (CL/F), respectively, were 8850 and 21.2 L/h. The CL/F decreased 1-2-fold for each 10 mg dose increase, whereas the different formulations caused a decrease in V/F of 66.6%. Sex was found to affect bioavailability (F), which decreased F by 47.5%. Females had higher F values than males. This PPK model described data from patients with psychosis who received paroxetine immediate-release tablets (IR-T) and/or sustained-release tablets (SR-T). Paroxetine trough concentrations and relative bioavailability were different between formulations and sex. The altered serum concentrations of paroxetine resulting from individual variants and additive effects need to be considered, to optimize the dosage regimen for individual patients.
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- 2022
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6. Computer-aided diagnosis of cervical dysplasia using colposcopic images
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Jing-Hang Ma, Shang-Feng You, Ji-Sen Xue, Xiao-Lin Li, Yi-Yao Chen, Yan Hu, and Zhen Feng
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Cervical dysplasia ,colposcopy ,computer-aided diagnosis ,multi-modal machine learning ,feature extraction - classification ensemble ,Neoplasms. Tumors. Oncology. Including cancer and carcinogens ,RC254-282 - Abstract
Backgroundcomputer-aided diagnosis of medical images is becoming more significant in intelligent medicine. Colposcopy-guided biopsy with pathological diagnosis is the gold standard in diagnosing CIN and invasive cervical cancer. However, it struggles with its low sensitivity in differentiating cancer/HSIL from LSIL/normal, particularly in areas with a lack of skilled colposcopists and access to adequate medical resources.Methodsthe model used the auto-segmented colposcopic images to extract color and texture features using the T-test method. It then augmented minority data using the SMOTE method to balance the skewed class distribution. Finally, it used an RBF-SVM to generate a preliminary output. The results, integrating the TCT, HPV tests, and age, were combined into a naïve Bayes classifier for cervical lesion diagnosis.Resultsthe multimodal machine learning model achieved physician-level performance (sensitivity: 51.2%, specificity: 86.9%, accuracy: 81.8%), and it could be interpreted by feature extraction and visualization. With the aid of the model, colposcopists improved the sensitivity from 53.7% to 70.7% with an acceptable specificity of 81.1% and accuracy of 79.6%.Conclusionusing a computer-aided diagnosis system, physicians could identify cancer/HSIL with greater sensitivity, which guided biopsy to take timely treatment.
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- 2022
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7. Associaton of Retinol Binding Protein 4 (RBP4) Levels With Hyperuricemia: A Cross-Sectional Study in a Chinese Population
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Guo-bao Hong, Xiao-fei Shao, Jia-min Li, Qin Zhou, Xiao-Su Ke, Pei-Chun Gao, Xiao-Lin Li, Jing Ning, Hai-Shan Chen, Hua Xiao, Chong-Xiang Xiong, and Hequn Zou
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retinol binding protein ,hyperuricemia ,prediction ,cross-sectional survey ,risk ,Diseases of the endocrine glands. Clinical endocrinology ,RC648-665 - Abstract
BackgroundThere are few studies on predictive biomarkers for hyperuricemia, and the predictive value of these biomarkers tends to be poor. Additionally, no reports have described the predictive value of retinol binding protein 4 (RBP4) for hyperuricemia.PurposeThis study was performed to evaluate the value of RBP4 for predicting the risk of hyperuricemia in a general population, determine whether RBP4 could be used alone or in combination with other factors to predict the risk of hyperuricemia in the general population, and establish an optimum predictive model.MethodsWe conducted a population-based cross-sectional survey in 2018, involving a questionnaire, physical examination, and laboratory testing. We enrolled 2303 individuals by stratified random sampling, and 2075 were included in the data analysis after applying the eligibility criteria.ResultsSerum RBP4 level had a highly significant association with hyperuricemia (P
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- 2022
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8. Crocetin: A Systematic Review
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Zi-Liang Guo, Mao-Xing Li, Xiao-Lin Li, Peng Wang, Wei-Gang Wang, Wei-Ze Du, Zhi-Qiang Yang, Sheng-Fu Chen, Di Wu, and Xiu-Yu Tian
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crocetin ,crocetin derivatives ,distribution ,pharmacological activity ,pharmacokinetics ,toxicity ,Therapeutics. Pharmacology ,RM1-950 - Abstract
Crocetin is an aglycone of crocin naturally occurring in saffron and produced in biological systems by hydrolysis of crocin as a bioactive metabolite. It is known to exist in several medicinal plants, the desiccative ripe fruit of the cape jasmine belonging to the Rubiaceae family, and stigmas of the saffron plant of the Iridaceae family. According to modern pharmacological investigations, crocetin possesses cardioprotective, hepatoprotective, neuroprotective, antidepressant, antiviral, anticancer, atherosclerotic, antidiabetic, and memory-enhancing properties. Although poor bioavailability hinders therapeutic applications, derivatization and formulation preparation technologies have broadened the application prospects for crocetin. To promote the research and development of crocetin, we summarized the distribution, preparation and production, total synthesis and derivatization technology, pharmacological activity, pharmacokinetics, drug safety, drug formulations, and preparation of crocetin.
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- 2022
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9. A Simple and Sensitive HPLC-MS/MS Assay for the Quantitation of Blonanserin and N-Desethyl Blonanserin in Rat Plasma and Its Application to Pharmacokinetic Study
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Shan-qing Huang, Xiao-lin Li, Zhan-zhang Wang, Hao-yang Lu, Tao Xiao, Xiao-jia Ni, Shu-jing Liu, Ming Zhang, De-wei Shang, and Yu-guan Wen
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Analytical chemistry ,QD71-142 - Abstract
A high-performance liquid chromatographic method coupled with triple quadrupole mass spectrometry (LC-MS/MS) for the analysis of blonanserin and its active metabolite, N-desethyl blonanserin, in rat plasma has been developed and validated. The biological samples were treated by simple direct protein precipitation before separation on an Agilent Eclipse Plus C18 column (4.6 × 100 mm, 3.5 μm) with a column temperature of 35°C at a flow rate of 0.5 mL/min. The mobile phase A is a mixture of methanol and water (75 : 25, v/v, 5 mM ammonium formate), and the mobile phase B is acetonitrile containing 0.1% formic acid. The ratio of mobile phase A to mobile phase B is 15 : 85. Electrospray ionization (ESI) multiple reaction monitoring modes are used for detection, which are m/z 368.10 ⟶ 296.90 (blonanserin), m/z 340.15 ⟶ 297.05(N-desethyl blonanserin), and m/z 348.15⟶ 302.05 (N-desethyl blonanserin-d8). The linear response range was 0.1–100.0 ng/mL for blonanserin and N-desethyl blonanserin. The lower limit of quantification (LLOQ), calibration curves, carryover, and matrix effects were sufficiently accurate and precise according to the National Medical Products Administration (NMPA) guidelines for bioanalytical method validation. This analytical method was successfully applied in a blonanserin-poloxamer thermosensitive gel pharmacokinetic study in rats.
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- 2022
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10. A multi-component PSO algorithm with leader learning mechanism for structural damage detection.
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Xiao-Lin Li, Roger Serra, and Julien Olivier
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- 2022
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11. High-fat diet aggravates colitis via mesenteric adipose tissue derived exosome metastasis-associated lung adenocarcinoma transcript 1
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Dong Chen, Miao-Miao Lu, Jin-Hai Wang, Yue Ren, Ling-Ling Xu, Wei-Xin Cheng, Sai-Sai Wang, Xiao-Lin Li, Xiao-Fei Cheng, Jian-Guo Gao, Farhin Shaheed Kalyani, and Xi Jin
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Gastroenterology ,General Medicine - Published
- 2022
12. Complications and Treatments of Pseudomonas aeruginosa Infection After Rhinoplasty With Implants: A Clinical Study
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Qi, Zeng, Yun-Gang, Hu, Yu-Xi, Tang, Bao-Fu, Yu, and Xiao-Lin, Li
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Otorhinolaryngology ,Surgery ,General Medicine - Abstract
Pseudomonas aeruginosa is an opportunistic pathogen, and because of its specificity, its treatments appear tricky in postrhinoplasty infections with internal implants. This study summarizes the clinical characteristics and treatment of this type of infections to provide some reference for clinical work.We retrospectively analyzed 10 patients who were diagnosed with a nasal infection of P. aeruginosa after implant nasal augmentation. The results of the bacterial culture and drug sensitivity test of the patients' wound secretions were summarized and analyzed. We summarized the characteristics of the patients' infection and the treatments, and we also summarized the patients' prognosis.In these 10 cases, their implants included rib cartilage and ear cartilage alone, as well as their own cartilage combined with expanded polytetrafluoroethylene and silicone. All patients developed wound infections within 1 month after rhinoplasty, with bacterial cultures of P. aeruginosa . Prolonged use of sensitive antibiotics, as well as wound dressing changes, failed to keep the infection well under control. Patients whose implant was removed and thoroughly debrided within 1 week of infection did not experience any serious complications. In patients who were infected for1 week before surgery to remove the implants, complications such as nasal column necrosis and nasal contracture occurred, and later the nasal repair was performed after multiple surgeries.For bacterial infections in postrhinoplasty wounds with implants, we recommend early bacterial culture. If the infection is clearly P. aeruginosa , the implant should be removed and thoroughly debrided as soon as possible to avoid serious complications.Level IV.
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- 2022
13. The determining role of carbon addition on mechanical performance of a non-equiatomic high-entropy alloy
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Xiao-lin Li, Xiao-xiao Hao, Haifeng Wang, Zhaodong Wang, Qi Wang, Xiangtao Deng, and Chi Jin
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Materials science ,Polymers and Plastics ,Mechanical Engineering ,Metals and Alloys ,Slip (materials science) ,Plasticity ,Solid solution strengthening ,Precipitation hardening ,Deformation mechanism ,Mechanics of Materials ,Ultimate tensile strength ,Materials Chemistry ,Ceramics and Composites ,Deformation (engineering) ,Composite material ,Necking - Abstract
The mechanical properties and deformation mechanism of a C-doped interstitial high-entropy alloy (iHEA) with a nominal composition of Fe49.5Mn29.7Co9.9Cr9.9C1 (at. %) were investigated. An excellent combination of strength and ductility was obtained by cold rolling and annealing. The structure of the alloy is consisted of FCC matrix and randomly distributed Cr23C6. For gaining a better understanding of deformation mechanism, EBSD and TEM were conducted to characterize the microstructure of tensile specimens interrupted at different strains. At low strain (2%), deformation is dominated by dislocations and their partial slip. With the strain increase to 20%, deformation-driven athermal phase transformation and dislocations slip are the main deformation mechanism. While at high strain of 35% before necking, deformation twins have been observed besides the HCP phase. The simultaneous effect of phase transformation (TRIP effect) and mechanical twins (TWIP effect) delay the shrinkage, and improve the tensile strength and plasticity. What's more, compared with the HEA without C addition, the yield strength of the C-doped iHEA has been improved, which can be attributed to the grain refinement strengthening and precipitation hardening. Together with the lattice friction and solid solution strengthening, the theoretical calculated values of yield strength match well with the experimental results.
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- 2022
14. Unveiling the in-situ hydrogen intercalation in Mo2COx for promoting the alkaline hydrogen evolution reaction
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Hong Chuan Fu, Xiao Hui Chen, Bo Yang, Yuan Hao Luo, Ting Li, Xiao Hu Wang, Qing Zhang, Xiao Lin Li, Nian Bing Li, and Hong Qun Luo
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Process Chemistry and Technology ,Catalysis ,General Environmental Science - Published
- 2023
15. Tuning the d-band center of NiC2O4 with Nb2O5 to optimize the Volmer step for hydrazine oxidation-assisted hydrogen production
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Xiao Hui Chen, Hong Chuan Fu, Li Li Wu, Xiao Lin Li, Bo Yang, Ting Li, Fei Gu, Jing Lei Lei, Nian Bing Li, and Hong Qun Luo
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Environmental Chemistry ,Pollution - Abstract
Nb2O5 can adjust the electronic structure of SNiC2O4 and improve the catalytic activity. Replacing the OER with the HzOR for hydrogen production can save 80% of energy consumption.
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- 2022
16. Excited state biexcitons in monolayer WSe2 driven by vertically grown graphene nanosheets with high-density electron trapping edges
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Bo Wen, Da-Ning Luo, Ling-Long Zhang, Xiao-Lin Li, Xin Wang, Liang-Liang Huang, Xi Zhang, and Dong-Feng Diao
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Physics and Astronomy (miscellaneous) - Published
- 2023
17. Exosomes derived from mesenchymal stem cells overexpressing miR-210 inhibits neuronal inflammation and contribute to neurite outgrowth through modulating microglia polarization
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Qing-hua Xiong, Lei Zhao, Guan-qun Wan, Yun-gang Hu, and Xiao-lin Li
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General Medicine - Abstract
Inflammatory responses play a critical role in the progress of neurodegenerative disorders. MSC-Exos is considered to have an anti-inflammatory effect on the treatment strategy for brain injury. However, the therapeutic effect and possible mechanism of Exosomal miR-210 on microglia polarization-induced neuroinflammation and neurite outgrowth have not been reported. MSC-Exos were isolated by ultracentrifugation, identified by Nanosight NS300, transmission electron microscopy, and western bolt. In vitro, to explore the protective mechanism of MSC-Exos against neuroinflammation, the microglial BV2 cell was exposed to lipopolysaccharide to assess inflammatory changes. The intake of 1,1’-dioctadecyl-3,3,3’,3’-tetramethylindocarbocyanine perchlorate (Dil)-MSC-Exos into microglia was observed by fluorescence microscopy. The results showed that Exosomal miR-210 treatment significantly inhibited the production of nitric oxide and pro-inflammatory cytokines. Exosomal miR-210 treatment also increased the number of M2 microglia cells and inhibited M1 microglia polarization. In addition, western blot demonstrated that Exosomal miR-210 reduced neuronal apoptosis. Thus, Exosomal miR-210 attenuated neuronal inflammation and promoted neurite outgrowth. Exosomal miR-210 from MSCs attenuated neuronal inflammation and contributed to neurogenesis possibly by inhibiting microglial M1 polarization.
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- 2023
18. Effectiveness of repetitive transcranial magnetic stimulation on post-stroke unilateral spatial neglect: a systematic review and meta-analysis
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Xiao-Lin Li, Xiao-Dan Liu, Bo Chen, Zhi-Xing Zhou, and Chunlei Shan
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- 2023
19. GARP: a family of glycine and alanine-rich proteins that helps spider mites feed on plants
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Qin‐Zhe Sun, Xiao‐Lin Li, Yu‐Fei Shi, Yan‐Chun Zhang, Wen‐Jie Chai, Ruo‐Yu Chen, Jinzhi Niu, and Jin‐Jun Wang
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Insect Science ,Agronomy and Crop Science ,General Biochemistry, Genetics and Molecular Biology ,Ecology, Evolution, Behavior and Systematics - Abstract
Spider mites (Tetranychidae) are destructive agricultural pests that have evolved strategies to overcome plant defenses, such as the ability to puncture the leaves of their hosts to feed. The expression of many genes with unknown functions is altered during feeding, but little is known about the role of these genes in plant-mite interactions. Here, we identified three novel gene families through analysis of genomic and transcriptomic data from three spider mite species. These GARP family genes encode glycine and alanine-rich proteins; they are present in mites (Acariformes) but absent in ticks (Parasitiformes) in the subclass Acari, indicating that these genes have undergone a significant expansion in spider mites and thus play important adaptive roles. Transcriptomic analysis revealed that the expression of GARP genes is strongly correlated with feeding and the transfer to new hosts. We used RNA interference to silence GARP1d in the two-spotted spider mite Tetranychus urticae, which inhibited feeding and egg laying and significantly increased mortality when the mites were transferred to soybean shoots; a similar effect was observed after TuVATPase was silenced. However, no changes in mite mortality were observed after TuGARP1d-silenced mites were placed on an artificial diet, which was different from the effect of TuVATPase silencing. Our results indicate that GARP family members play important roles in mite-plant interactions. Additional studies are needed to clarify the mechanisms underlying these interactions. This article is protected by copyright. All rights reserved.
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- 2022
20. [Comparison of distribution of verbascoside in normoxic and hypoxic rats]
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Mao-Xing, Li, Wei-Gang, Wang, Xiao-Lin, Li, and Peng, Wang
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Glucosides ,Phenols ,Animals ,Polyphenols ,Hypoxia ,Chromatography, High Pressure Liquid ,Rats - Abstract
This study established a high performance liquid chromatography(HPLC) method for the simultaneous determination of verbascoside(VB) and its main metabolite caffeic acid(CA) in rat tissue samples. A low-pressure low-oxygen animal experimental chamber was used to simulate the plateau environment for establishing the hypoxic rat model. After intragastric administration of 300 mg·kg~(-1) VB, the normoxic and hypoxic rats were sacrificed for the collection of heart, liver, spleen, lung, kidney, brain, muscle, large intestine, small intestine, and stomach tissue samples at the time points of 30, 60, and 90 min. VB and CA concentrations in each tissue sample were measured by HPLC, and the distribution of VB and CA in normoxic and hypoxic rats was compared. The results showed that after intragastric administration, VB can be rapidly absorbed and distributed into various tissues including brain in both normoxic and hypoxic rats, indicating that VB can pass through the blood-brain barrier. In the gastrointestinal tract, VB was mainly distributed in small intestine, which suggested that the main absorption site of VB was small intestine. A large amount of VB was detected in muscle and lung, and only a small amount in other tissues. CA was detected in other tissues except brain, heart, and muscle. Small intestine had the most abundant CA, followed by stomach, large intestine, and kidney, and only a small amount of CA was detected in the liver, spleen, and lung(lt;50 ng·mL~(-1)). The results indicated that VB may be mainly absorbed and metabolized in the gastrointestinal tract to produce CA and was possibly excreted through kidney. Compared with normoxic rats, hypoxic rats had reduced and slow distribution of VB and increased ratio of VB concentration in tissue to plasma, which implied that the relative proportion of VB from systemic circulation to tissues was increased in hypoxic rats. This study provides a basis for the application of VB in anti-hypoxia therapy and for the formulation of anti-hypoxia dosing regimens.
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- 2022
21. Population pharmacokinetics and dosing optimization of olanzapine in Chinese paediatric patients: Based on the impact of sex and concomitant valproate on clearance
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Tao Xiao, Jin‐Qing Hu, Shu‐Jing Liu, Hui‐Qin Lu, Xiao‐Lin Li, Wan Kong, Shan‐Qing Huang, Xiu‐Qing Zhu, Ming Zhang, Hao‐Yang Lu, Xiao‐Jia Ni, Han‐Lun Yang, De‐Wei Shang, and Yu‐Guan Wen
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Pharmacology ,Adult ,Male ,China ,Valproic Acid ,Models, Biological ,Kinetics ,Olanzapine ,Humans ,Pharmacology (medical) ,Female ,Child ,Aged ,Antipsychotic Agents - Abstract
Olanzapine is an atypical antipsychotic drug used for mental disorders. There are limited studies providing sufficient pharmacokinetic data, thus the variability of concentrations of olanzapine used in Chinese paediatric patients aged 10 to 17 years remains to be evaluated.Therapeutic drug monitoring data were collected from 151 paediatric patients aged 10 to 17 years who received olanzapine. The model was developed with a NONMEM software program. The final model validation and evaluation were assessed by bootstrap, diagnostic scatter plots, and normalized prediction distribution error (NPDE). Regimens of different dosages were simulated to reach the target concentration levels of 20 ng/ml, by using the final model with typical parameters.The one-compartment model was considered the best fit for the data. Typical estimates of the absorption rate constant (Ka), apparent clearance (CL/F), and apparent distribution volume (V/F) in the final model were 0.142 hOur results identified concomitant valproate and sex as significant covariates in olanzapine population pharmacokinetics. Our model may be a useful tool for recommending dosage adjustments for physicians. The pharmacokinetics of olanzapine in patients aged 10 to 17 years was generally similar to that of adults and the elderly.
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- 2022
22. Modulating the interfacial built-in electric field in oxygen vacancies-enriched Ru/MxOy@C (M = V, Nb, Ta) ordered macroporous heterojunctions for electrocatalytic hydrogen production
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Xiao Hui Chen, Hong Chuan Fu, Xiao Lin Li, Ting Li, Qing Zhang, Zi Qing Li, Yuan Hao Luo, Jing Lei Lei, Nian Bing Li, and Hong Qun Luo
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General Chemical Engineering ,Environmental Chemistry ,General Chemistry ,Industrial and Manufacturing Engineering - Published
- 2023
23. Mass spectrometry imaging and single-cell transcriptional profiling reveal the tissue-specific regulation of bioactive ingredient biosynthesis in Taxus leaves
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Xiaori Zhan, Tian Qiu, Hongshan Zhang, Hou Kailin, Xueshuang Liang, Cheng Chen, Zhijing Wang, Qicong Wu, Xiaojia Wang, Xiao-lin Li, Mingshuang Wang, Shangguo Feng, Houqing Zeng, Chunna Yu, Huizhong Wang, and Chenjia Shen
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Cell Biology ,Plant Science ,Molecular Biology ,Biochemistry ,Biotechnology - Published
- 2023
24. Controlling particle transitions with weak periodic perturbations
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Yan-Hong Qin, Ling-Zheng Meng, Xiao-Lin Li, Guo-Guo Xin, and Li-Chen Zhao
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- 2022
25. Effects of Altitude and Duration of Differing Levels of Hypoxic Exposure on Hypoxia-Inducible Factor-1α in Rat Tissues
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Xiao-lin Li, Wei-gang Wang, Mao-xing Li, Tian-long Liu, Xiu-yu Tian, and Lan Wu
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Physiology ,Altitude ,Public Health, Environmental and Occupational Health ,Animals ,General Medicine ,RNA, Messenger ,Rats, Wistar ,Hypoxia ,Hypoxia-Inducible Factor 1, alpha Subunit ,Rats - Abstract
Li, Xiao-lin, Wei-gang Wang, Mao-xing Li, Tian-long Liu, Xiu-yu Tian, and Lan Wu. Effects of altitude and duration of differing levels of hypoxic exposure on hypoxia-inducible factor-1α in rat tissues.
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- 2022
26. Determination of the concentration of prepared Rehmannia root solution by fluorescence polarization spectroscopy combined with the least-square method
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Xiao-Lin Li, Yun-Ling Sun, Hai-Ying Ma, Jie Wang, Hang Yang, and Chun Zhu
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Statistical and Nonlinear Physics ,Condensed Matter Physics - Abstract
In recent years, the safety of traditional Chinese medicine has gradually attracted consumers’ attention. Therefore, the qualitative and quantitative detection of traditional Chinese medicine has become a hot research topic in recent years. In this paper, we chose prepared Rehmannia root as the research object. By using the fluorescence polarization spectrum and the least-square method, a method for predicting the concentration of traditional Chinese medicine solutions was established. First, we scanned the fluorescence polarization spectra of the prepared Rehmannia root solution to obtain the inner anisotropy and anisotropy of each sample. Then, the least-square method was used to obtain the functional relationship between concentration and anisotropy. Finally, the functional relation was applied to predict the concentration of the prepared Rehmannia root solution. The correlation coefficients of the predicted concentration were 0.9944 and 0.9928, with an average recovery rate of 100.94% and 99.58%, respectively. The results show that fluorescence polarization spectroscopy combined with the least-squares method can quickly and accurately detect the concentration of Rehmannia glutinosa root solution. This provides a new method for the content detection of traditional Chinese medicine components.
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- 2022
27. Effect of aqueous extract of Astragalus membranaceus on behavioral cognition of rats living at high altitude
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Xing, Du, Tian-Long, Liu, Wen-di, Tao, Mao-Xing, Li, Xiao-Lin, Li, and Lan, Yan
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Mammals ,Cognition ,Altitude ,Malondialdehyde ,Animals ,Humans ,Astragalus propinquus ,Hippocampus ,Rats - Abstract
To investigate the effect of aqueous extract of Astragalus membranaceus on cognitive ability of rats living at high altitude.Rats were exposed to a simulated highaltitude hypobaric hypoxia chamber. The behavior of rats was tested by eight-arm maze. The contents of malondialdehyde (MDA), glutathione (GSH), reactive oxygen species (ROS) and activity of total superoxide dismutase (T-SOD) in hippocampus were measured. The expressions of mammalian target of rapamycin (mTOR) and cleaved capase-3 in hippocampus were determined by reverse transcription-polymerase chain reaction and Western blot.The behavioral cognitive ability of the hypoxic control group was significantly lower than that of the normoxic control group. Under hypoxic environment, after the administration of aqueous extract of Astragalus membranaceus, the behavioral cognitive ability of rats was significantly improved. In hippocampal tissue, the content of MDA and ROS were significantly decreased, while the content of GSH and activity of T-SOD in hippocampus were significantly increased. The mRNA expression of mTOR and P70S6K and the protein expression of p-mTOR were significantly increased; the mRNA expression of 4E-binding protein 1 (4E-BP1) and the protein expression of phosphorylated-4E-BP1 (p-4EBP1) and cleaved capase-3 were significantly decreased.When the rats are exposed to high altitude hypoxia, the behavioral cognitive ability could be significantly reduced. Aqueous extract of Astragalus membranaceus can significantly improve cognitive function in rats under hypoxia. The potential mechanism is related to improving oxidative stress, reducing the accumulation of free radicals and metabolites, and activating mTOR signaling pathway.
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- 2022
28. Engineered BMSCs-Derived Exosomal miR-542-3p Promotes Cutaneous Wound Healing
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Xiao-Lin Li, Qing-Hua Xiong, Lei Zhao, Guan-Qun Wan, and Yun-Gang Hu
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Endocrinology, Diabetes and Metabolism ,Immunology and Allergy - Abstract
Background: The healing of cutaneous wounds requires better strategies, which remain a challenge. Previous reports indicated that the therapeutic function of mesenchymal stem cells is mediated by exosomes. This work demonstrated the regenerative effects of engineered BMSCsderived Exosomal miR-542-3p in skin wound mouse models. Methods: Bone marrow mesenchymal stem cells (BMSCs) -derived exosomes (BMSCs-Exos) were isolated by ultracentrifugation and identified by Transmission Electron Microscope (TEM) and Nanoparticle Tracking Analysis (NTA). BMSCs-Exo was loaded with miRNA-542-3p by electroporation. We explored the effects of miRNA-542-3p-Exo on the proliferation and migration of Human Skin Fibroblasts (HSFs)/Human dermal microvascular endothelial cells (HMECs). In addition, The angiogenesis of HMECs was detected by Tube formation assay in vitro. The effects of miRNA-542-3p-Exo in the skin wound mouse model were detected by H&E staining, Masson staining, and immunofluorescence analysis. We assessed the effect of miRNA-542-3p-Exo on collagen deposition, new blood vessel formation, and wound remodeling in a skin wound mouse model. Results: MiRNA-542-3p-Exos could be internalized by HSFs/HMECs and enhance the proliferation, migration, and angiogenesis of HSFs/HMECs in vitro and in vivo. The protein expression of collagen1/3 was significantly increased after miRNA-542-3p-Exo treatment in HSFs. In addition, the local injection of miRNA-542-3p-Exo promoted cellular proliferation, collagen deposition, neovascularization, and accelerated wound closure. Conclusion: This study suggested that miRNA-542-3p-Exo can stimulate HSFs/HMECs function. The treatment of miRNA-542-3p-Exo in the skin wound mouse model significantly promotes wound repair. The therapeutic potential of miRNA-542-3p-Exo may be a future therapeutic strategy for cutaneous wound healing.
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- 2022
29. Untargeted metabolomics combined with pseudotargeted lipidomics revealed the metabolite profiles of blood-stasis syndrome in type 2 diabetes mellitus
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Li Liu, Yuan-bin Liang, Xiao-lin Liu, Hong-qin Wang, Yi-fei Qi, Min Wang, Bao-xin Chen, Qing-bing Zhou, Wen-xin Tong, and Ying Zhang
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Type 2 diabetes mellitus (T2DM) ,Blood-stasis syndrome (BSS) ,Untargeted metabolomics ,Pseudotargeted lipidomics ,Glycerophospholipids (GPs) ,Fatty acyls (FAs) ,Science (General) ,Q1-390 ,Social sciences (General) ,H1-99 - Abstract
Objective: Blood-stasis syndrome (BSS), an important syndrome in Type 2 diabetes mellitus(T2DM), is associated with the pathophysiological mechanisms underlying diabetic vascular complications. However, BSS has not been fully characterized as of yet. Due to the strong correlation between BSS and vasculopathy, we hypothesized that the metabolic characteristics of BSS in T2DM (T2DM BSS) are highly specific. By combining untargeted metabolomics and pseudotargeted lipidomics approaches, this study aimed to comprehensively elucidate the metabolic traits of T2DM BSS, thereby providing novel insights into the vascular complications of diabetes and establishing a foundation for precision medicine. Methods: The survey was conducted in Haidian District of Beijing from October 2021 to November 2021, and data collection was completed in January 2022. Liquid chromatography-mass spectrometry (LC-MS) based untargeted metabolomics and liquid chromatography-tandem mass spectrometry (LC-MS/MS) based pseudotargeted lipidomics were performed to detect metabolites and lipids. Multivariate, univariate, and pathway analyses were utilized to investigate metabolic changes. The unique metabolites of BSS were obtained by inter-group comparisons and screening. Receiver operating characteristic (ROC) curve analysis was performed to evaluate the diagnostic accuracy of metabolites. Results: A total of 1189 participants completed the survey, of which 120 participants were recruited in this study and further divided into a discovery cohort (n = 90) and a validation cohort (n = 30). Among these, 21 participants were selected for psuedotargeted lipidomics analysis. 81 metabolites, mainly involving glycerophospholipids, were identified as unique metabolites of T2DM BSS, while fatty acyls (FAs) were identified as unique lipids. T2DM BSS was associated with significant dysregulation in glycerophospholipid metabolism and choline metabolism within cancer pathways as major metabolic disturbances. Furthermore, analyses of both the discovery and validation cohorts, indicated that LysoPC (20:5(5Z,8Z,11Z,14Z,17Z)/0:0) and LysoPC (15:0) had the greatest impact on distinguishing BSS. Conclusion: Altered levels of glycerophospholipids and FAs have been associated with T2DM BSS. These results provide valuable mechanistic insights linked with the development of BSS in T2DM subjects.
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- 2024
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30. Role of female-predominant MYB39-bHLH13 complex in sexually dimorphic accumulation of taxol in Taxus media
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Chunna Yu, Jiefang Huang, Qicong Wu, Chengchao Zhang, Xiao-lin Li, Xinyun Xu, Shangguo Feng, Xiaori Zhan, Zhehao Chen, Huizhong Wang, and Chenjia Shen
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Genetics ,Plant Science ,Horticulture ,Biochemistry ,Biotechnology - Abstract
Taxus trees are major natural sources for the extraction of taxol, an anti-cancer agent used worldwide. Taxus media is a dioecious woody tree with high taxol yield. However, the sexually dimorphic accumulation of taxoids in T. media is largely unknown. Our study revealed high accumulation of taxoids in female T. media trees using a UPLC–MS/MS method. Thereafter, many differential metabolites and genes between female and male T. media trees were identified using metabolomic and transcriptomic analyses, respectively. Most of the taxol-related genes were predominantly expressed in female trees. A female-specific R2R3-MYB transcription factor gene, TmMYB39, was identified. Furthermore, bimolecular fluorescence complementation and yeast two-hybrid assays suggested the potential interaction between TmMYB39 and TmbHLH13. Several taxol biosynthesis-related promoter sequences were isolated and used for the screening of MYB recognition elements. The electrophoretic mobility shift assay indicated that TmMYB39 could bind to the promoters of the GGPPS, T10OH, T13OH, and TBT genes. Interaction between TmMYB39 and TmbHLH13 transactivated the expression of the GGPPS and T10OH genes. TmMYB39 might function in the transcriptional regulation of taxol biosynthesis through an MYB-bHLH module. Our results give a potential explanation for the sexually dimorphic biosynthesis of taxol in T. media.
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- 2022
31. Potential Mechanism of Maslinic Acid against Hypolipidemia Based on Network Pharmacology and Molecular Docking Integration Strategy
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Fei-Fei Che, Yi Liang, Hong Li, Wen Chen, Xiao Lin Li, and Li Zhi Zhao
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- 2022
32. Bicyclol Alleviates Atherosclerosis by Manipulating Gut Microbiota
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Xiao‐Lin Li, Jin‐Jin Cui, Wen‐Sheng Zheng, Jin‐Lan Zhang, Rui Li, Xiao‐Lei Ma, Miao Lin, Hui‐Hui Guo, Cong Li, Xiao‐You Yu, Peng Du, Li‐Min Zhao, Shuwang He, Pei Lan, Jian‐Dong Jiang, Yongsheng Che, and Lu‐Lu Wang
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Biomaterials ,Mice, Inbred C57BL ,Mice ,Biphenyl Compounds ,Animals ,Dysbiosis ,General Materials Science ,General Chemistry ,Atherosclerosis ,Biotechnology ,Gastrointestinal Microbiome - Abstract
Atherosclerosis (AS) is associated with high morbidity and mortality, thus imposing a growing burden on modern society. Herb-derived bicyclol (BIC) is a versatile bioactive compound that can be used to treat AS. However, its efficacy in AS is not yet described. Here, it is shown that BIC normalizes gut microflora dysbiosis induced by a high fat diet in Apoe
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- 2021
33. High-fat diet aggravates colitis
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Dong, Chen, Miao-Miao, Lu, Jin-Hai, Wang, Yue, Ren, Ling-Ling, Xu, Wei-Xin, Cheng, Sai-Sai, Wang, Xiao-Lin, Li, Xiao-Fei, Cheng, Jian-Guo, Gao, Farhin Shaheed, Kalyani, and Xi, Jin
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Mice ,MicroRNAs ,Adipose Tissue ,Animals ,RNA, Long Noncoding ,Obesity ,Colitis ,Diet, High-Fat ,Exosomes ,Activating Transcription Factor 6 - Abstract
Obesity is associated with an increased risk of developing Crohn's disease (CD), higher disease activity, and comparatively worse clinical outcomes.To investigate the role of mesenteric adipose tissue-derived exosomes in the pathogenesis of CD aggravation in obese individuals.First, we induced colitis in mice initiated on high-fat and normal diets and compared the severity of colitis. We then extracted and identified exosomes from mesenteric adipose tissue and determined the levels of metastasis-associated lung adenocarcinoma transcript 1 (MALAT1) in mesenteric adipose tissue-derived exosomes and the colon. Next, we demonstrated an interaction between MALAT1 and the miR-15a-5p/activating transcription factor 6 (ATF6) axis. Finally, we explored the effects of mesenteric adipose tissue-derived exosomes extracted from mice fed a high-fat or normal diet on the severity of 2,4,6-trinitrobe-nzenesulfonic acid (TNBS)-induced colitis and ATF6-related endoplasmic reticulum stress pathways.High-fat diet was found to aggravate TNBS-induced colitis in mice. The expression of MALAT1 in mesenteric adipose tissue-derived exosomes of high-fat diet-fed mice increased. The increased expression of MALAT1 in colon tissue exacerbated TNBS-induced colitis and activated the ATF6 endoplasmic reticulum stress pathway. This effect was partially reversed by the reduced expression of MALAT1 and overexpression of miR-15a-5p.Mesenteric adipose tissue-derived exosome-encapsulated long noncoding RNAs MALAT1 targets the colon and aggravates TNBS-induced colitis in obese mice, which may potentially act on the miR-15a-5p/ATF6 axis and activate endoplasmic reticulum stress.
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- 2021
34. Economic policy uncertainty and shadow banking: Firm-level evidence from China
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Deng-Kui Si, Shen Wan, Xiao-Lin Li, and Dongmin Kong
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Business, Management and Accounting (miscellaneous) ,Finance - Published
- 2022
35. Shadow banking business and firm risk-taking: Evidence from China
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Deng-Kui Si and Xiao-Lin Li
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Business, Management and Accounting (miscellaneous) ,Finance - Published
- 2022
36. Role of female-predominant MYB39-bHLH13 complex in sexually dimorphic accumulation of taxol in
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Chunna, Yu, Jiefang, Huang, Qicong, Wu, Chengchao, Zhang, Xiao-Lin, Li, Xinyun, Xu, Shangguo, Feng, Xiaori, Zhan, Zhehao, Chen, Huizhong, Wang, and Chenjia, Shen
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- 2021
37. ThermomiR-377-3p-induced suppression of Cirbp expression is required for effective elimination of cancer cells and cancer stem-like cells by hyperthermia
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Tao-Yan Lin, Jun-Shuang Jia, Wei-Ren Luo, Xiao-Lin Lin, Sheng-Jun Xiao, Jie Yang, Jia-Wei Xia, Chen Zhou, Zhi-Hao Zhou, Shu-Jun Lin, Qi-Wen Li, Zhi-Zhi Yang, Ye Lei, Wen-Qing Yang, Hong-Fen Shen, Shi-Hao Huang, Sheng-Chun Wang, Lin-Bei Chen, Yu-Lin Yang, Shu-Wen Xue, Yong-Long Li, Guan-Qi Dai, Ying Zhou, Ying-Chun Li, Fang Wei, Xiao-Xiang Rong, Xiao-Jun Luo, Bing-Xia Zhao, Wen-Hua Huang, Dong Xiao, and Yan Sun
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Hyperthermia ,Nasopharyngeal carcinoma (NPC) ,Cold-inducible RNA-binding protein (Cirbp) ,Temperature-sensitive miRNA-377-3p (termed thermomiR-377-3p) ,Cancer stem cells (CSCs) ,Chemotherapy ,Neoplasms. Tumors. Oncology. Including cancer and carcinogens ,RC254-282 - Abstract
Abstract Background In recent years, the development of adjunctive therapeutic hyperthermia for cancer therapy has received considerable attention. However, the mechanisms underlying hyperthermia resistance are still poorly understood. In this study, we investigated the roles of cold‑inducible RNA binding protein (Cirbp) in regulating hyperthermia resistance and underlying mechanisms in nasopharyngeal carcinoma (NPC). Methods CCK-8 assay, colony formation assay, tumor sphere formation assay, qRT-PCR, Western blot were employed to examine the effects of hyperthermia (HT), HT + oridonin(Ori) or HT + radiotherapy (RT) on the proliferation and stemness of NPC cells. RNA sequencing was applied to gain differentially expressed genes upon hyperthermia. Gain-of-function and loss-of-function experiments were used to evaluate the effects of RNAi-mediated Cirbp silencing or Cirbp overexpression on the sensitivity or resistance of NPC cells and cancer stem-like cells to hyperthermia by CCK-8 assay, colony formation assay, tumorsphere formation assay and apoptosis assay, and in subcutaneous xenograft animal model. miRNA transient transfection and luciferase reporter assay were used to demonstrate that Cirbp is a direct target of miR-377-3p. The phosphorylation levels of key members in ATM-Chk2 and ATR-Chk1 pathways were detected by Western blot. Results Our results firstly revealed that hyperthermia significantly attenuated the stemness of NPC cells, while combination treatment of hyperthermia and oridonin dramatically increased the killing effect on NPC cells and cancer stem cell (CSC)‑like population. Moreover, hyperthermia substantially improved the sensitivity of radiation‑resistant NPC cells and CSC‑like cells to radiotherapy. Hyperthermia noticeably suppressed Cirbp expression in NPC cells and xenograft tumor tissues. Furthermore, Cirbp inhibition remarkably boosted anti‑tumor‑killing activity of hyperthermia against NPC cells and CSC‑like cells, whereas ectopic expression of Cirbp compromised tumor‑killing effect of hyperthermia on these cells, indicating that Cirbp overexpression induces hyperthermia resistance. ThermomiR-377-3p improved the sensitivity of NPC cells and CSC‑like cells to hyperthermia in vitro by directly suppressing Cirbp expression. More importantly, our results displayed the significantly boosted sensitization of tumor xenografts to hyperthermia by Cirbp silencing in vivo, but ectopic expression of Cirbp almost completely counteracted hyperthermia-mediated tumor cell-killing effect against tumor xenografts in vivo. Mechanistically, Cirbp silencing-induced inhibition of DNA damage repair by inactivating ATM-Chk2 and ATR-Chk1 pathways, decrease in stemness and increase in cell death contributed to hyperthermic sensitization; conversely, Cirbp overexpression-induced promotion of DNA damage repair, increase in stemness and decrease in cell apoptosis contributed to hyperthermia resistance. Conclusion Taken together, these findings reveal a previously unrecognized role for Cirbp in positively regulating hyperthermia resistance and suggest that thermomiR-377-3p and its target gene Cirbp represent promising targets for therapeutic hyperthermia.
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- 2024
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38. The driving forces of China's business cycles: Evidence from an estimated DSGE model with housing and banking
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Xinyu Ge, Xiao-Lin Li, Yong Li, and Yan Liu
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Economics and Econometrics ,Finance - Published
- 2022
39. The impact of exchange rate policy uncertainty shock on Chinese energy firms' risk-taking
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Xiao-Lin Li, Hui Ding, and Guojing Qiu
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Economics and Econometrics ,General Energy ,Exchange rate ,Economics ,Monetary economics ,Risk taking ,Energy (signal processing) ,Shock (mechanics) - Published
- 2022
40. ZIF-8@GMP-Tb nanocomplex for ratiometric fluorescent detection of alkaline phosphatase activity
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Yu Ling, Lei Han, Chu Chu Wan, Hong Qun Luo, Xiao Hu Wang, Lin Zhao He, Zi Yi Xu, Nian Bing Li, and Xiao Lin Li
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Guanosine Monophosphate ,chemistry.chemical_element ,Terbium ,Conjugated system ,Alkaline Phosphatase ,Fluorescence ,Atomic and Molecular Physics, and Optics ,Analytical Chemistry ,chemistry.chemical_compound ,Linear range ,chemistry ,Guanosine monophosphate ,Imidazolate ,Zeolites ,Humans ,Alkaline phosphatase ,Luminescence ,Instrumentation ,Spectroscopy ,Fluorescent Dyes ,Nuclear chemistry - Abstract
Luminescent metal–organic frameworks (LMOFs) and their functional materials with unique characteristics can provide the basis for the construction of new analytical techniques, which can meet the continuous demand for various fields. In this work, guanosine monophosphate (GMP), terbium ion (Tb3+) and zeolitic imidazolate framework-8 (ZIF-8) are self-assembled to form a ZIF-8@GMP-Tb nanocomplex, which can be utilized as a ratiometric fluorescent probe to monitor alkaline phosphatase (ALP) activity. Specifically, with adding ALP, the fluorescence intensity at 547 nm (one of the characteristic emission peaks of Tb3+) obviously decreased. Meanwhile, the conjugated structure of GMP increased the fluorescence of ZIF-8 (located at 330 nm). The possible mechanism was proposed through the characterization of the materials. Based on the variation of the emission peaks at 330 and 547 nm, the ratiometric fluorescent sensor of ALP has a linear range of 0.25–20 U/L. Moreover, applying this sensing system to the detection of ALP in the human serum sample and ALP inhibitor investigation possesses satisfactory results. This work provides a new perspective for the utilization of ZIF-8 and lanthanide ions in manufacturing simple and sensitive sensors.
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- 2022
41. A decellularized nerve matrix scaffold inhibits neuroma formation in the stumps of transected peripheral nerve after peripheral nerve injury
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Shuai Qiu, Pei-Jun Deng, Fu-Lin He, Li-Wei Yan, Zhe-Hui Tu, Xiao-Lin Liu, Da-Ping Quan, Ying Bai, Can-Bin Zheng, and Qing-Tang Zhu
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decellularized nerve matrix scaffold ,extracellular matrix ,fibrosis ,functional recovery ,microarchitecture ,microenvironment ,pain ,peripheral nerve ,tissue remodeling ,traumatic neuroma ,Neurology. Diseases of the nervous system ,RC346-429 - Abstract
Traumatic painful neuroma is an intractable clinical disease characterized by improper extracellular matrix (ECM) deposition around the injury site. Studies have shown that the microstructure of natural nerves provides a suitable microenvironment for the nerve end to avoid abnormal hyperplasia and neuroma formation. In this study, we used a decellularized nerve matrix scaffold (DNM-S) to prevent against the formation of painful neuroma after sciatic nerve transection in rats. Our results showed that the DNM-S effectively reduced abnormal deposition of ECM, guided the regeneration and orderly arrangement of axon, and decreased the density of regenerated axons. The epineurium-perilemma barrier prevented the invasion of vascular muscular scar tissue, greatly reduced the invasion of α-smooth muscle actin-positive myofibroblasts into nerve stumps, effectively inhibited scar formation, which guided nerve stumps to gradually transform into a benign tissue and reduced pain and autotomy behaviors in animals. These findings suggest that DNM-S-optimized neuroma microenvironment by ECM remodeling may be a promising strategy to prevent painful traumatic neuromas.
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- 2023
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42. Reconstituted LH2 in multilayer membranes induced by poly-l-lysine: Structure of supramolecular and electronic states
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Xiao-Lin Liu, Yuan-Yuan Hu, Kang Li, Ming-Qing Chen, and Peng Wang
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Multi-layer structure of membrane ,Light-harvesting complex 2 ,Structural splitting of B850 exciton ,Photoprotection ,Chemistry ,QD1-999 - Abstract
To explore the effect of cell membrane stacking on the function of light-harvesting complex 2 (LH2) in purple nonsulfur photosynthetic bacteria, LH2 from Rhodobacter sphaeroides 2.4.1 (R. sph 2.4.1) was reconstituted into lipid bilayer vesicles (LH2@liposome) and further formed multi-layer structure by electrostatic interaction with poly-l-lysine (LH2@liposome/PLL), which was characterized by cryo-electron microscopy (cryo-EM) and TEM. When embedded in liposomes and additionally in multi-layer liposomes, the absorption band, zero-crossing point of CD signals and fluorescence emission of LH2 B850 excitons were observed to uniformly have 1–2 nm red-shifting. Combining with the corresponding fluorescence quench and the generation of shorter-living fluorescence species, a new excitonic species generated through B850 structural splitting was proposed. By FT-Raman and triplet carotenoid dynamics, the structural mechanism was deduced and discussed. Briefly, all environmental changes, including LH2 aggregating and multi-layer membrane stacking, eventually applied forces on B850 exciton molecules mainly through the hydrogen bonding between the C3-acetyl carbonyl groups of B850 BChls and Tyr44 and 45 residues at C-terminus of α-polypeptides. The effect of multi-layer structure on LH2 could be assigned as a kind of photoprotection.
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- 2023
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43. Escherichia fergusonii Promotes Nonobese Nonalcoholic Fatty Liver Disease by Interfering With Host Hepatic Lipid Metabolism Through Its Own msRNA 23487Summary
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Feng-Zhi Xin, Ze-Hua Zhao, Xiao-Lin Liu, Qin Pan, Zi-Xuan Wang, Lin Zeng, Qian-Ren Zhang, Lin Ye, Meng-Yu Wang, Rui-Nan Zhang, Zi-Zhen Gong, Lei-Jie Huang, Chao Sun, Feng Shen, Lu Jiang, and Jian-Gao Fan
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Steatosis ,Gut Microbiota ,Lipid Metabolism ,Peroxisome Proliferator-Activated Receptor α ,Diseases of the digestive system. Gastroenterology ,RC799-869 - Abstract
Background & Aims: Gut microbiota and microbial factors regulate the pathogenesis of nonalcoholic fatty liver disease (NAFLD) in patients with obesity and metabolic abnormalities, but little is known about their roles in nonobese NAFLD. Expansion of Escherichia is associated with NAFLD pathogenesis. We aimed to investigate the pathogenic role of Escherichia fergusonii and its products in the development of nonobese NAFLD. Methods: We characterized the intestinal microbiome signature in a cohort of NAFLD patients and healthy controls by 16S ribosomal RNA sequencing. The role of E fergusonii was estimated in rats after 16 weeks of administration, and features of NAFLD were assessed. E fergusonii–derived microRNA-sized, small RNAs (msRNAs) were analyzed by deep sequencing. Results: We detected an expansion of Escherichia_Shigella in NAFLD patients compared with healthy controls, and its increase was associated with disease severity independent of obesity. E fergusonii, a member of the genus Escherichia, induced the development of nonobese NAFLD characterized by hepatic steatosis and hepatocyte ballooning in rats without obesity. It disturbed host lipid metabolism by inhibiting hepatic lipid β-oxidation and promoting de novo lipogenesis. We also showed that E fergusonii caused the development of hepatic inflammation and fibrosis in a sizable fraction of animals at an advanced stage of NAFLD. Mechanistically, E fergusonii–derived msRNA 23487 down-regulated host hepatic peroxisome proliferator-activated receptor α expression, which could contribute to lipid accumulation in the liver. Conclusions: These results suggest that E fergusonii promotes the pathogenesis of steatohepatitis and fibrosis in nonobese rats by secreting msRNA 23487, and it might be a potential biomarker for predicting steatohepatitis in nonobese NAFLD.
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- 2022
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44. Rhodobacter sphaeroides as a model to study the ecotoxicity of 1-alkyl-3-methylimidazolium bromide
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Xiao-Lin Liu, Ming-Qing Chen, Yang-Lin Jiang, Rong-Yao Gao, Ze-Jun Wang, and Peng Wang
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Rhodobacter sphaeroides ,ionic liquids ,cytotoxicity ,membrane integrity ,spectral probe ,Biology (General) ,QH301-705.5 - Abstract
The purple non-sulfur bacterium Rhodobacter sphaeroides was selected as a biological model to investigate its response to the toxicity of 1-alkyl-3-methylimidazolium bromide ([Cnmim]Br), a type of ionic liquid (IL), with different alkyl chain lengths (n describes the number of carbon atoms in the alkyl chain). The inhibition of bacterial growth by [Cnmim]Br was positively correlated with n. Morphological characterization revealed that [Cnmim]Br caused cell membrane perforation. The signal amplitude of the electrochromic absorption band shift of endogenous carotenoids showed a negatively linear correlation with n, and the amplitude of the blue-shift of the B850 band in light-harvesting complex 2 showed a positively linear correlation with n. Furthermore, an increase in blocked ATP synthesis and increase in antioxidant enzyme activity were observed in chromatophores treated with ILs containing longer alkyl chains. In summary, the purple bacterium can be developed as a model to monitor ecotoxicity and examine the mechanism of IL toxicity.
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- 2023
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45. Intelligence profiles and adaptive behaviors of high-functioning autism spectrum disorder and developmental speech and language disorders
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Wen-Yuan Jin, Ling-Ling Wu, Li-Fei Hu, Wen-Hao Li, Chao Song, Yan-Yan Wang, Xiao-Lin Liu, and Zhi-Wei Zhu
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high-functioning autism spectrum disorder ,developmental speech and language disorders ,intelligence ,adaptive behavior ,WISC ,ABAS ,Pediatrics ,RJ1-570 - Abstract
ObjectiveThe present study was aimed at investigating the intelligence profiles and adaptive behaviors of children with high-functioning autism spectrum disorder (HFASD) and developmental speech and language disorders (DSLDs). We compared the similarities and differences of cognitive capabilities and adaptive functions and explored their correlations in the HFASD and DSLDs groups.Methods128 patients with HFASD, 111 patients with DSLDs and 114 typically developing (TD) children were enrolled into our study. Wechsler Intelligence Scale for Children-IV (WISC-IV) and Adaptive Behavior Assessment System-II (ABAS-II) were respectively applied to evaluate intelligence profiles and adaptive behaviors. Intelligence quotient (IQ) scores and adaptive functioning scores among the HFASD, DSLDs and TD groups were compared through one-way ANOVA. Pearson correlation coefficient was applied to examine the relationships between WISC indices and ABAS domains.ResultsOutcomes showed significantly poorer intelligence profiles and adaptive behaviors in HFASD and DSLDs groups. Both children with HFASD and DSLDs demonstrated impairments in verbal comprehension and executive functions. Processing speed and working memory were the predominant defects of children with HFASD and DSLDs in the field of executive functions, respectively. Whereas perceptual reasoning was a relative strength for them. Children with DSLDs had balanced scores of all the domains in ABAS-II; nevertheless, HFASD individuals demonstrated striking impairments in Social domain. Correlation analysis showed IQs of children with HFASD were positively correlated with all the domains and General Adaptive Composite (GAC) of ABAS-II. Additionally, IQs were positively correlated with Conceptual domain and GAC for children with DSLDs. Compared with DSLDs group, intelligence displayed stronger correlations with adaptive behaviors in HFASD group.ConclusionOur study expanded insights regarding intelligence profiles and adaptive behaviors of children with HFASD and DSLDs. Moreover, this study made breakthroughs in discovering positive correlations between IQs and adaptive functions in the two neurodevelopmental disorders.
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- 2023
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46. A machine learning-based diagnostic model for children with autism spectrum disorders complicated with intellectual disability
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Chao Song, Zhong-Quan Jiang, Li-Fei Hu, Wen-Hao Li, Xiao-Lin Liu, Yan-Yan Wang, Wen-Yuan Jin, and Zhi-Wei Zhu
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artificial intelligence ,machine learning ,child ,autism spectrum disorder ,intellectual disability ,diagnostic model ,Psychiatry ,RC435-571 - Abstract
BackgroundEarly detection of children with autism spectrum disorder (ASD) and comorbid intellectual disability (ID) can help in individualized intervention. Appropriate assessment and diagnostic tools are lacking in primary care. This study aims to explore the applicability of machine learning (ML) methods in diagnosing ASD comorbid ID compared with traditional regression models.MethodFrom January 2017 to December 2021, 241 children with ASD, with an average age of 6.41 ± 1.96, diagnosed in the Developmental Behavior Department of the Children’s Hospital Affiliated with the Medical College of Zhejiang University were included in the analysis. This study trained the traditional diagnostic models of Logistic regression (LR), Support Vector Machine (SVM), and two ensemble learning algorithms [Random Forest (RF) and XGBoost]. Socio-demographic and behavioral observation data were used to distinguish whether autistic children had combined ID. The hyperparameters adjustment uses grid search and 10-fold validation. The Boruta method is used to select variables. The model’s performance was evaluated using discrimination, calibration, and decision curve analysis (DCA).ResultAmong 241 autistic children, 98 (40.66%) were ASD comorbid ID. The four diagnostic models can better distinguish whether autistic children are complicated with ID, and the accuracy of SVM is the highest (0.836); SVM and XGBoost have better accuracy (0.800, 0.838); LR has the best sensitivity (0.939), followed by SVM (0.952). Regarding specificity, SVM, RF, and XGBoost performed significantly higher than LR (0.355). The AUC of ML (SVM, 0.835 [95% CI: 0.747–0.944]; RF, 0.829 [95% CI: 0.738–0.920]; XGBoost, 0.845 [95% CI: 0.734–0.937]) is not different from traditional LR (0.858 [95% CI: 0.770–0.944]). Only SVM observed a good calibration degree. Regarding DCA, LR, and SVM have higher benefits in a wider threshold range.ConclusionCompared to the traditional regression model, ML model based on socio-demographic and behavioral observation data, especially SVM, has a better ability to distinguish whether autistic children are combined with ID.
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- 2022
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47. Population Pharmacokinetics and Initial Dosage Optimization of Tacrolimus in Pediatric Hematopoietic Stem Cell Transplant Patients
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Xiao-Lin Liu, Yan-Ping Guan, Ying Wang, Ke Huang, Fu-Lin Jiang, Jian Wang, Qi-Hong Yu, Kai-Feng Qiu, Min Huang, Jun-Yan Wu, Dun-Hua Zhou, Guo-Ping Zhong, and Xiao-Xia Yu
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tacrolimus ,pediatric HSCT patients ,population pharmacokinetics ,Bayesian estimation ,dosage simulation ,Therapeutics. Pharmacology ,RM1-950 - Abstract
Background: There is a substantial lack of tacrolimus pharmacokinetic information in pediatric hematopoietic stem cell transplant (HSCT) patients. This study aimed to develop population pharmacokinetics (PopPK) of tacrolimus in pediatric HSCT patients and to devise model-guided dosage regimens.Methods: A retrospective analysis was performed on 86 pediatric HSCT patients who received tacrolimus intravenously or orally. A total of 578 tacrolimus trough concentrations (C0) were available for pharmacokinetic analysis using a non-linear mixed-effects modeling method. Demographic and clinical data were included and assessed as covariates via the stepwise method. Bayesian estimators were used to devise pediatric dosage regimens that targeted C0 of 5–15 ng mL−1.Results: A one-compartment model with first-order absorption adequately described the tacrolimus pharmacokinetics. Clearance (CL), volume of distribution (V), and typical bioavailability (F) in this study were estimated to be 2.42 L h−1 (10.84%), 79.6 L (16.51%), and 19% (13.01%), respectively. Body weight, hematocrit, post-transplantation days, and caspofungin and azoles concomitant therapy were considered significant covariates for tacrolimus CL. Hematocrit had a significant impact on the V of tacrolimus. In the subgroup cohort of children (n = 24) with CYP3A5 genotype, the clearance was 1.38-fold higher in CYP3A5 expressers than in non-expressers. Simulation indicated that the initial dosage optimation of tacrolimus for intravenous and oral administration was recommended as 0.025 and 0.1 mg kg−1 d−1 (q12h), respectively.Conclusion: A PopPK model for tacrolimus in pediatric HSCT patients was developed, showing good predictive performance. Model-devised dosage regimens with trough tacrolimus concentrations provide a practical strategy for achieving the therapeutic range.
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- 2022
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48. Corannulene-based hole-transporting material for efficient and stable perovskite solar cells
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Ming-Wei An, Bao-Shan Wu, Shun Wang, Zuo-Chang Chen, Yin Su, Lin-Long Deng, Shu-Hui Li, Zi-Ang Nan, Han-Rui Tian, Xiao-Lin Liu, Da-Qin Yun, Qianyan Zhang, Su-Yuan Xie, and Lan-Sun Zheng
- Subjects
Physics ,QC1-999 - Abstract
Summary: Core structures with linear, planar, and spiral conformations have been designed as triphenylamine (TPA)-based hole-transporting materials (HTMs), which are the most prevalent small molecular HTMs in perovskite solar cells (PSCs). However, most of the reported TPA-based HTMs cannot achieve sufficient balance between efficiency and stability, which is governed by core structures. Herein, a sym-penta(N,N-bis(4-methoxyphenyl)aniline)corannulene (cor-OMePTPA) featuring a corannulene core and five TPA peripheral arms is designed as alternative HTM. Planar negative-intrinsic-positive (n-i-p) PSCs with cor-OMePTPA exhibit champion efficiencies of 20% and maintain 86% of their initial performances for more than 1,000 h after thermal annealing at 60°C. Compared with spiro-OMeTAD, cor-OMePTPA-based PSCs show slightly lower efficiencies but much better thermal stabilities. The main merit of cor-OMePTPA lies in its bimolecular interpenetration capability with noncovalent interactions to modulate the HTM configurations from single-molecular curvature to bimolecular planarity, thereby providing promising opportunities to achieve excellent balance between efficiency and stability.
- Published
- 2021
- Full Text
- View/download PDF
49. A Pilot Study of the Effect of Lactobacillus casei Obtained from Long-Lived Elderly on Blood Biochemical, Oxidative, and Inflammatory Markers, and on Gut Microbiota in Young Volunteers
- Author
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Li-Hua Mei, Wen-Xuan Zheng, Zheng-Tao Zhao, Ning Meng, Qin-Ren Zhang, Wen-Jun Zhu, Rui-Ding Li, Xiao-Lin Liang, and Quan-Yang Li
- Subjects
probiotics ,Lactobacillus casei LTL1879 ,healthy young people ,blood biochemical ,oxidant ,inflammation ,Nutrition. Foods and food supply ,TX341-641 - Abstract
Probiotic intake has been shown to improve certain physiological health indicators. We aimed to examine effects of Lactobacillus casei LTL1879, obtained from long-lived elderly volunteers, on blood biochemical, oxidative, and inflammatory markers and gut microbiota in twenty healthy, young volunteers. Volunteers were randomly divided into equal probiotic and placebo groups and changes in blood biochemical indicators, oxidative and inflammatory markers, and gut microbiota were examined after three weeks of probiotic intervention. The probiotic group’s antioxidant levels were significantly enhanced post-intervention. Total antioxidant capacity (T-AOC) levels were significantly increased (p < 0.0001), while malondialdehyde (MDA) levels decreased (p < 0.05), and total superoxide dismutase (T-SOD) levels increased, but with no significant difference. In addition, Interleukin-10 (IL-10) and tumor necrosis factor-α (TNF-α) levels were significantly up-regulated and down-regulated (p < 0.05, respectively). Escherichia coli, Enterococcus, and Bacteroides expression was significantly reduced (p < 0.05), while Clostridium leptum, Bifidobacterium, and Lactobacillus expression increased (p < 0.05). Volunteer health status was quantified using principal components and cluster analysis, indicating that the probiotic group’s overall score was higher than that of the placebo group. The results of this pilot study suggest L. casei LTL 1879 can significantly improve specific immune, oxidative, and gut microbiota characteristics related to health factors.
- Published
- 2021
- Full Text
- View/download PDF
50. Visual Analysis Method of Blockchain Community Evolution Based on DPoS Consensus Mechanism
- Author
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WEN Xiao-lin, LI Chang-lin, ZHANG Xin-yi, LIU Shang-song, ZHU Min
- Subjects
blockchain ,dpos ,community evolution ,consensus mechanism ,visual analysis ,Computer software ,QA76.75-76.765 ,Technology (General) ,T1-995 - Abstract
DPoS (delegated proof of stake) is one of the current mainstream blockchain consensus mechanisms,and the unique node election mechanism makes it form an evolving blockchain community.Analyzing the evolution model of the blockchain community can discover the potential risks of the consensus mechanism,which has very important research significance.For the DPoS consensus mechanism blockchain data,a novel combination analysis method of the consensus mechanism effectiveness is proposed,and a set of visual analysis methods are designed to help users analyze the evolutionary model of the blockchain community from multiple angles.First,it quantifies the difference between the degree of completion of the work and the voting ranking before and after the node ranking change and analyzes the selection efficiency and incentive efficiency of the consensus mechanism;then,it focuses on the combined efficiency of the consensus mechanism,the evolution of the geographical distribution of nodes,and the comparison of the evolutionary differences between nodes and designs visual views and interactive means;finally,it designs and implements a visual analysis system of blockchain community evolution based on the DPoS consensus mechanism based on the real data of the EOS main chain and verifies the usability and effectiveness of this method through case studies and expert evaluation.
- Published
- 2022
- Full Text
- View/download PDF
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