13 results on '"Vibrio cholerae non-O1"'
Search Results
2. Bacteremia by non-O1/non-O139 Vibrio cholerae: Case description and literature review.
- Author
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Rodríguez, José Y., Duarte, Carolina, Rodríguez, Gerson J., Angeline Montaño, Lucy, Benítez-Peñuela, Miguel A., Díaz, Paula, López, Olga, and Álvarez-Moreno, Carlos A.
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VIBRIO cholerae ,LITERATURE reviews ,BACTEREMIA ,CHOLERA ,AGGLUTINATION tests ,POLYMERASE chain reaction - Abstract
Copyright of Biomédica: Revista del Instituto Nacional de Salud is the property of Instituto Nacional de Salud of Colombia and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)
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- 2023
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3. Death in a farmer with underlying diseases carrying Vibrio cholerae non-O1/non-O139 producing zonula occludens toxin
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Ying, Zhao, Tingting, He, Bowen, Tu, Xujian, Mao, Jingyi, Jiang, Xia, Jiang, Fengming, Wang, Min, Wang, Yu, Wang, and Hongyan, Sun
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Endotoxins ,Male ,Microbiology (medical) ,Cholera Toxin ,Vibrio cholerae non-O1 ,Farmers ,Infectious Diseases ,Cholera ,Humans ,General Medicine ,Middle Aged - Abstract
The non-O1/non-O139 Vibrio cholerae caused outbreaks or sporadic cases of gastroenteritis that was rarely seen in good sanitary condition. It was described a case of systemic multiple organ lesions that worsened because of non-O1/non-O139 V. cholerae, suggesting that serogroups have a potential virulence in enhancing pathogenicity with patients with underlying diseases compared with a healthy population.Samples are identified by strain culture, polymerase chain reaction (PCR) virulence identification, and whole genome sequencing.A middle-aged man was diagnosed with cytotoxin-producing and nontoxin V. cholerae non-O1/non-O139 serogroups. Although lacking the CT toxin encoded by ctxAB gene, the pathogenesis of cholera relies on the synergistic action of many other genes, especially virulence genes.This case suggested that the laborers engaging in agricultural production are at potential risk of V. cholerae infection by exposure of open wounds to contaminated water . However, epidemiological investigation should focus on the objective cause of the change of working environment. Furthermore, common diseases can possibly enhance the virulence of non-O1/non-O139 serogroups by attacking the tight junction of small intestinal epithelial cells, further triggering bacteremia, a process that may lead to death within 48-72 hours, which requires great attention.
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- 2022
4. Genomic and functional insights into antibiotic resistance genes floR and strA linked with the SXT element of Vibrio cholerae non-O1/non-O139.
- Author
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Saha M, Pragasam AK, Kumari S, Verma J, Das B, and Bhadra RK
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- Anti-Bacterial Agents pharmacology, Genomics, Chloramphenicol, Streptomycin, Drug Resistance, Microbial, Vibrio cholerae non-O1
- Abstract
The emergence and spread of antibiotic-resistant bacterial pathogens are a critical public health concern across the globe. Mobile genetic elements (MGEs) play an important role in the horizontal acquisition of antimicrobial resistance genes (ARGs) in bacteria. In this study, we have decoded the whole genome sequences of multidrug-resistant Vibrio cholerae clinical isolates carrying the ARG-linked SXT, an integrative and conjugative element, in their large chromosomes. As in others, the SXT element has been found integrated into the 5'-end of the prfC gene (which encodes peptide chain release factor 3 involved in translational regulation) on the large chromosome of V. cholerae non-O1/non-O139 strains. Further, we demonstrate the functionality of SXT-linked floR and strAB genes, which confer resistance to chloramphenicol and streptomycin, respectively. The floR gene-encoded protein FloR belongs to the major facilitator superfamily efflux transporter containing 12 transmembrane domains (TMDs). Deletion analysis confirmed that even a single TMD of FloR is critical for the export function of chloramphenicol. The floR gene has two putative promoters, P1 and P2. Sequential deletions reveal that P2 is responsible for the expression of the floR . Deletion analysis of the N- and/or C-terminal coding regions of strA established their importance for conferring resistance against streptomycin. Interestingly, qPCR analysis of the floR and strA genes indicated that both of the genes are constitutively expressed in V. cholerae cells. Further, whole genome-based global phylogeography confirmed the presence of the integrative and conjugative element SXT in non-O1/non-O139 strains despite being non-multidrug resistant by lacking antimicrobial resistance (AMR) gene cassettes, which needs monitoring.
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- 2024
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5. Study Findings on Vibrio cholerae Detailed by Researchers at Robert Koch Institute (Virulence and resistance patterns of Vibrio cholerae non-O1/non-O139 acquired in Germany and other European countries).
- Abstract
A recent study conducted by researchers at the Robert Koch Institute in Germany examined the virulence and resistance patterns of Vibrio cholerae non-O1/non-O139 strains in Germany and other European countries. The study found that global warming has led to an increase in the emergence of Vibrio species in various environments. The researchers analyzed 63 clinical and 24 environmental strains of Vibrio cholerae non-O1/non-O139 and identified high genetic diversity among the isolates. They also discovered the presence of antibiotic resistance genes, including the carbapenemase gene VCC-1, in some isolates. The study emphasizes the importance of monitoring antimicrobial resistance to protect public health. [Extracted from the article]
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- 2023
6. Bacteremia by non-O1/non-O139 Vibrio cholerae: Case description and literature review.
- Author
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Rodríguez JY, Duarte C, Rodríguez GJ, Montaño LA, Benítez-Peñuela MA, Díaz P, López O, and Álvarez-Moreno CA
- Subjects
- Humans, Polymerase Chain Reaction, Vibrio cholerae non-O1 genetics, Bacteremia diagnosis
- Abstract
Bacteremia by non-O1/non-O139 Vibrio cholerae is a rare entity associated with high mortality rates. We report a case of non-O1/non-O139 V. cholerae bacteremia confirmed by polymerase chain reaction and agglutination tests. The clinicoepidemiological characteristics and therapeutic options for this infection are also described.
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- 2023
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7. Genetic and mutational analysis of virulence traits and their modulation in an environmental toxigenic
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Quoelee, Biswas, Ayushi, Purohit, Ashok, Kumar, Dipayan, Rakshit, Diganta, Maiti, Bhabatosh, Das, and Rupak K, Bhadra
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Vibrio cholerae non-O1 ,Bacterial Proteins ,Cholera ,Virulence ,Humans ,Phylogeny - Published
- 2022
8. Etiology and clinical features of non-O1/non-O139 Vibrio cholerae infection in an inland city in China.
- Author
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Xiang YH, Hu QQ, Liu Y, Sheng R, Wang J, Li WJ, Shi J, Li X, and Lu SH
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- Humans, Aged, Retrospective Studies, Anti-Bacterial Agents therapeutic use, Cholera diagnosis, Vibrio cholerae non-O1, Bacteremia diagnosis
- Abstract
Non-O1/non-O139 Vibrio cholerae (NOVC) causes various illnesses ranging in severity from mild to life-threatening but were ignored previously. Knowledge of the NOVC infection, particularly bacteremia, is limited because of its rarity. Here we first retrospectively reported the demographic, clinical, and therapy characteristics of patients with NOVC infection. Isolated NOVC stains were identified by a series of biochemical, mass spectrometry (MS), and serum agglutination tests. The results of 11 patients with NOVC infection (including 8 with bacteremia) with a median age of 68 years were included in this report. Most isolated NOVC strains had antibiotic susceptibility. Patients with NOVC-positive were distributed in various departments, most occurring in gastroenterology (6 cases). Hepatic disease was the most common comorbid disease, followed by diabetes (3 cases) and biliary tract disease (3 cases). Two cases were previously healthy. The most common symptom at presentation was fever. All patients presented with abnormal changes in hematology and inflammatory parameters. Cephalosporins were the most frequently used antibiotics. Ten patients had a favorable outcome after treatment; one died from complicated underlying diseases. In summary, we recommend the timely identification of NOVC strains using MALDI-TOF-MS. The suspicion of NOVC bacteremia cannot be ruled out regardless of the host's immune status. An alternative therapeutic regimen for this infection may be β-lactam antibiotics or combined with β-lactamase inhibitors. Regardless, the specific therapeutic regimen should be based on the antibiogram data.
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- 2023
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9. Antimicrobial resistance among clinical Vibrio cholerae non-O1/non-O139 isolates: systematic review and meta-analysis.
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Wu Q, Vaziri AZ, Omidi N, Hassan Kaviar V, Maleki A, Khadivar P, and Kouhsari E
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- Humans, Anti-Bacterial Agents pharmacology, Anti-Bacterial Agents therapeutic use, Trimethoprim, Sulfamethoxazole Drug Combination, Drug Resistance, Bacterial, Ciprofloxacin, Tetracycline, Chloramphenicol, Kanamycin, Erythromycin, Gentamicins, Microbial Sensitivity Tests, Vibrio cholerae non-O1, Cholera drug therapy, Cholera epidemiology
- Abstract
Non-O1/non-O139 Vibrio cholerae (NOVC) are nonpathogenic or asymptomatic colonizers in humans, but they may be related to intestinal or extra-intestinal (severe wound infections or sepsis) infections in immunocompromised patients.The present study aimed to evaluate the weighted pooled resistance (WPR) rates in clinical NOVC isolates based on different years, areas, quality, antimicrobial susceptibility testing (AST), and resistance rates. We systematically searched the articles in PubMed, Scopus, and Embase (until January 2020). Data analyses were performed using the Stata software program (version 17). A total of 16 studies that had investigated 824 clinical NOVC isolates were included in the meta-analysis. The majority of the studies were conducted in Asia (n = 14) and followed by Africa (n = 2). The WPR rates were as follows: erythromycin 10%, ciprofloxacin 5%, cotrimoxazole 27%, and tetracycline 13%. There was an increase in resistance to ciprofloxacin, nalidixic acid, and gentamicin, norfloxacin during the period from 2000 to 2020. On the contrary, there was a decreased resistance to erythromycin, tetracycline, chloramphenicol, cotrimoxazole, ampicillin, streptomycin, kanamycin, and neomycin during the period from 2000 to 2020. The lowest resistance rate were related to gentamicin, kanamycin, ciprofloxacin, and chloramphenicol against NOVC strains. However, temporal changes in antimicrobial resistance rate were found in our study. We established continuous surveillance, careful appropriate AST, and limitations on improper antibiotic usage, which are essential, especially in low-income countries.
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- 2023
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10. Transcriptome-wide identification and characterization of the Macrobrachium rosenbergii microRNAs potentially related to immunity against non-O1 Vibrio cholerae infection.
- Author
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Zhang L, Zhang Z, Xu S, Zhang X, and Liu X
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- Animals, Transcriptome, Palaemonidae, Cholera, Vibrio cholerae non-O1, MicroRNAs genetics, MicroRNAs metabolism
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Non-O1 Vibrio cholerae, a member of the Vibrio family, could cause gastrointestinal infection of Macrobrachium rosenbergii and result in significant economic losses. However, few studies on microRNA immunity related to non-O1 V. cholerae infection of M. rosenbergii. The aim of this study was to elucidate the mechanism of miRNA in the potential immune response of M. rosenbergii. to non-O1 V. cholerae MSVC-GY01 infection by transcriptome sequencing. Following quality screening, the control group received 10, 616, 712 clean reads, whereas the infected group received 9,727,616. The miRNA sequences in the two samples are extremely consistent and have a length of roughly 23 nt. In all, 871 known miRNAs were discovered, with 279 differentially expressed miRNAs (DEMs). Meanwhile, 62 novel miRNAs were predicted, including 43 DEMs. In order to understand the immune-related biological functions of DEMs, target genes were predicted. Pathway function annotation analysis showed that non-O1 V. cholerae affected the NOD-like receptor signaling pathway, RIG-I-like receptor signaling pathway, and Toll-like receptor signaling pathway, suggesting that miRNAs in the hepatopancreas play a key role in immune responses to pattern recognition receptors. Twelve DEMs were randomly selected for Quantitative Real-time PCR (qRT-PCR). Overall, the expression trends of qRT-PCR were consistent with the sequencing results. These findings corroborate the immunomodulatory function of miRNA in M. rosenbergii against non-O1 V. cholerae infection and provide guidance for the prevention and treatment of related illnesses., Competing Interests: Declaration of competing interest There are no conflicts of interest declared by the authors., (Copyright © 2023 Elsevier Ltd. All rights reserved.)
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- 2023
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11. Temporal-Spatial Distribution of Vibrio cholerae in Cuba: July 1997-December 2019.
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Fernández-Abreu A, Bravo-Fariñas L, Águila-Sánchez A, Cruz-Infante Y, Falcón-Márquez R, Toledo-Romaní ME, León-Venero MLÁ, Hernández-Martínez JL, Baldoquín-Rodríguez W, and Germán-Almeida ÁM
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- Child, Adolescent, Humans, Child, Preschool, Cross-Sectional Studies, Cuba epidemiology, Diarrhea epidemiology, Vibrio cholerae, Cholera epidemiology
- Abstract
Introduction: Vibrio cholerae is a microorganism that causes acute diarrheal diseases and cholera, one of the leading causes of global morbidity and mortality, especially in children under five years old. It is present in many regions and has been isolated from diverse sources such as water, soil and food. Surveillance of this microorganism in Cuba from 1985 through June 1997 showed circulation of non-epidemic non-O1/non-O139 serogroups, but surveillance continued to identify distribution of V. cholerae serotypes and serogroups in the different geographic regions of the country during the following years, due to the risk of introducing cholera-causing serogroups that provoked cholera epidemics in other countries of the region., Objective: Describe the temporal‒spatial distribution of serogroups and serotypes of V. cholerae in Cuba., Methods: A cross-sectional study was conducted that included isolates from passive surveillance of V. cholerae in 16 hygiene and epidemiology centers throughout Cuba from July 1997 through December 2019, submitted to the National Reference Laboratory for Acute Diarrheal Diseases of the Pedro Kourí Tropical Medicine Institute in Havana, Cuba. The timeline was subdivided into three five-year periods and one eight-year period. The centers submitting isolates were grouped into three geographical regions: western, central and eastern Cuba. A total of 1060 V. cholerae isolates were studied, from the 1438 samples sent from 15 Provincial Hygiene, Epidemiology and Microbiology Centers and the Municipal Hygiene, Epidemiology and Microbiology Center of the Isle of Youth Special Municipality. Genus, species and serotype of all specimens were studied and reviewed in the context of the outbreaks of acute diarrheal diseases reported in the country., Results: All 1060 isolates were confirmed as V. cholerae. In the distribution by time period and region, the highest percentage occurred in the 2012‒2019 period, and the eastern region contributed the most isolates in all periods. Approximately 63.9% (677/1060) were from outbreaks, and in the 2012‒2019 period, the most epidemic-causing isolates came from the western region. Approximately 52.8% (560/1060) were identified as non-O1/non-O139 V. cholerae, and 47.2% (500/1060) as O1 V. cholerae; of these, 96.4% (482/500) corresponded to Ogawa serotype and 3.6% (18/500) to Inaba. Circulation of non-O1/non-O139 V. cholerae occurred throughout the entire period. The O1 serogroup began to circulate in 2012 and continued through 2016; however, since 2017, it has not been identified again. In the western region, there were smaller percentages of isolates of non-O1/non-O139 V. cholerae in all periods, except 2012‒2019. In that period, V. cholerae O1 was identified to a lesser degree in the central region., Conclusions: Vibrio cholerae circulated in all three Cuban regions during the years studied, with a higher percentage of isolates of the non-O1/non-O139 serogroup, which caused outbreaks or sporadic cases of diarrhea in the eastern region, with the exception of the 2012‒2019 period, when epidemic outbreaks of the O1 serogroup (which causes cholera) occurred in all three regions, with higher percentages in the western region.
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- 2022
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12. Molecular identification and pathogenicity study of virulent Vibrio cholerae non O1/O139 serotype associated with mortality of farmed Labeo rohita (Hamilton, 1822), in India
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Basanta Kumar Das, Prasenjit Paria, Vikash Kumar, Pranaya Kumar Parida, Bijay Kumar Behera, Manoharmayum Shaya Devi, and Praveen Maurye
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Serotype ,Necrosis ,biology ,Virulence ,Aquatic Science ,biology.organism_classification ,medicine.disease_cause ,Microbiology ,Labeo ,Vibrio cholerae non-O1 ,Vibrio cholerae ,medicine ,medicine.symptom ,Gene ,Bacteria - Abstract
Vibrio cholerae is a gram-negative bacteria known for causing epidemics and pandemics conditions, comprised of both non-pathogenic and pathogenic strains. The present study identified a non O1, non-O139 V. cholerae (EMM1) serotype isolated from diseased Labeo rohita cultured in a wetland system at Purba Medinipur district, West Bengal, India. Identification of the isolate was done by biochemical test, 16S rRNA gene sequencing and phylogenetic analysis. The in vivo challenge experiment revealed that intraperitoneal injection of EMM1 serotype at a dose of 1.04× 107 CFU/ml resulted in 100% mortality of L. rohita. Furthermore, the presence of hlyA and rtxA virulent genes, confirmed by sequencing, in V. cholerae (EMM1) serotype might be, atleast partially involved in the mortality of L. rohita. In histological analysis, we found that EMM1 serotype challenge had a significant effect in L. rohita like degenerated hepatocytes in the liver, shrunken glomeruli in the kidney, necrosis of the intestinal villi epithelium and degeneration of muscle bundle were observed. Overall, the study demonstrated that, V. cholerae (EMM1) serotype is a potent aquatic microbial pathogen and draws the attention for proper management to prevent the possible occurrence of disease in aquaculture.
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- 2022
13. Non O1/ O139 Vibrio Cholerae Septicemia in a Patient with Hepatocellular Carcinoma.
- Author
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Sachu A, Johnson D, Thomas S, and Mathew R
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- Aged, Humans, Male, Carcinoma, Hepatocellular complications, Liver Neoplasms complications, Sepsis complications, Shock, Septic, Vibrio cholerae non-O1
- Abstract
Background: An elderly male with underlying Hepatocellular carcinoma came with history of fall with head and ear trauma, vomiting, abdominal pain, fatigue. Patient died within hours due to Septic shock Blood culture grew Non O1/O139 Vibrio cholerae which was later found to be non-toxigenic. This was a lethal case of non-O1/non-O139 V. cholerae sepsis and we focus on the dilemmas in identification and management of this rare bacterium., (© 2021 Arun S, et al.)
- Published
- 2021
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