1. Lumbar spine intrathecal transplantation of neural precursor cells promotes oligodendrocyte proliferation in hot spots of chronic demyelination
- Author
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Dimitra Mitsiadou, Nikolaos Grigoriadis, Steven Petratos, Paschalis Theotokis, Olympia Damianidou, Marina Boziki, Anastasia Chatzidimitriou, and Evangelia Kesidou
- Subjects
Pathology ,medicine.medical_specialty ,Encephalomyelitis, Autoimmune, Experimental ,Multiple Sclerosis ,Pathology and Forensic Medicine ,Cell therapy ,Mice ,Neural Stem Cells ,In vivo ,Precursor cell ,medicine ,Animals ,Remyelination ,Cell Proliferation ,business.industry ,General Neuroscience ,Multiple sclerosis ,Experimental autoimmune encephalomyelitis ,Cell Differentiation ,medicine.disease ,Oligodendrocyte ,Transplantation ,Mice, Inbred C57BL ,Oligodendroglia ,medicine.anatomical_structure ,Bromodeoxyuridine ,Neurology (clinical) ,Neoplasm Recurrence, Local ,business - Abstract
Experimental autoimmune encephalomyelitis (EAE) is a basic and reliable model used to study clinical and pathological hallmarks of multiple sclerosis (MS) in rodents. Several studies suggest neural precursor cells (NPCs) as a significant research tool while reporting that transplanted NPCs are a promising therapeutic approach to treating neurological disorders, such as MS. The main objective was to approach a preclinical, in vivo scenario of oligodendrogenesis with NPCs, targeting the main chronic demyelinated lumbosacral milieu of EAE, via the least invasive delivery method which is lumbar puncture. We utilized MOG35-55 peptide to induce EAE in C57BL/6 mice and prior to the acute relapse, we intervened with either the traceable GFP+ cellular therapy or saline solution in the intrathecal space of their lumbar spine. A BrdU injection, which enabled us to monitor endogenous proliferation, marked the endpoint 50 days post-induction (50 dpi). Neuropathology with high-throughput, triple immunofluorescent, and transmission electron microscopy (TEM) data were extracted and analyzed. The experimental treatment attenuated the chronic phase of EAE (50 dpi; score
- Published
- 2021