Back to Search
Start Over
Lumbar spine intrathecal transplantation of neural precursor cells promotes oligodendrocyte proliferation in hot spots of chronic demyelination
- Source :
- Brain pathology (Zurich, Switzerland). 32(4)
- Publication Year :
- 2021
-
Abstract
- Experimental autoimmune encephalomyelitis (EAE) is a basic and reliable model used to study clinical and pathological hallmarks of multiple sclerosis (MS) in rodents. Several studies suggest neural precursor cells (NPCs) as a significant research tool while reporting that transplanted NPCs are a promising therapeutic approach to treating neurological disorders, such as MS. The main objective was to approach a preclinical, in vivo scenario of oligodendrogenesis with NPCs, targeting the main chronic demyelinated lumbosacral milieu of EAE, via the least invasive delivery method which is lumbar puncture. We utilized MOG35-55 peptide to induce EAE in C57BL/6 mice and prior to the acute relapse, we intervened with either the traceable GFP+ cellular therapy or saline solution in the intrathecal space of their lumbar spine. A BrdU injection, which enabled us to monitor endogenous proliferation, marked the endpoint 50 days post-induction (50 dpi). Neuropathology with high-throughput, triple immunofluorescent, and transmission electron microscopy (TEM) data were extracted and analyzed. The experimental treatment attenuated the chronic phase of EAE (50 dpi; score
- Subjects :
- Pathology
medicine.medical_specialty
Encephalomyelitis, Autoimmune, Experimental
Multiple Sclerosis
Pathology and Forensic Medicine
Cell therapy
Mice
Neural Stem Cells
In vivo
Precursor cell
medicine
Animals
Remyelination
Cell Proliferation
business.industry
General Neuroscience
Multiple sclerosis
Experimental autoimmune encephalomyelitis
Cell Differentiation
medicine.disease
Oligodendrocyte
Transplantation
Mice, Inbred C57BL
Oligodendroglia
medicine.anatomical_structure
Bromodeoxyuridine
Neurology (clinical)
Neoplasm Recurrence, Local
business
Subjects
Details
- ISSN :
- 17503639
- Volume :
- 32
- Issue :
- 4
- Database :
- OpenAIRE
- Journal :
- Brain pathology (Zurich, Switzerland)
- Accession number :
- edsair.doi.dedup.....9626d3437652d3b5babe877319ad6e02