28 results on '"Budakov, Dragana"'
Search Results
2. The Pesticide Market in Serbia
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Milić Dragan, Novaković Tihomir, Grahovac Mila, Budakov Dragana, Grahovac Jovana, Vlajkov Vanja, Loc Marta, and Tekić Dragana
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biopesticides ,pesticide market ,serbia ,Agriculture - Abstract
The use of plant protection products (PPPs) has become a social concern regarding food safety and quality, and risks to human health and the environment. Accordingly, the agricultural microbials market is a steadily growing sector of the global agriculture industry. This paper evaluates the market of basic groups of pesticides in Serbia (namely herbicides, fungicides and insecticides), as well as other collectively presented plant protection products, with a particular emphasis on the biopesticides segment. The official data of the Serbian Plant Protection Directorate for the period 2000-2020 served as a primary source of data. The movement of PPPs and active ingredients (AIs) imports into Serbia were analyzed separately. The average values of PPP quantities imported into Serbia were computed for the period under consideration. The time series variability was evaluated using the coefficient of variation, whereas the dynamics of PPP import movements was evaluated using the rate of change. In the overall structure of the imported pesticides in Serbia, herbicides remain the dominant product type (57.0%), followed by fungicides (27.5%), insecticides (10.5%), and other PPPs (5.0%). The import of AIs for the production of herbicides claimed a share of 79.8%, whereas the share of AIs for the fungicide production approximated to 12.8% (followed by AIs for the insecticide production (6.7%) and AIs for the other groups of pesticides (0.7%). Biopesticides comprise a small share of the total crop protection market in Serbia. Bioinsecticides were found to be imported into Serbia in largest quantities (followed by biofungicides, bioacaricides, and biobactericides). No imports of bioherbicides have been recorded. According to the official data on the import of PPPs in Serbia in the period 2000–2020, it can be concluded that there is a positive growth trend, which is expected to continue in the future. Although the import of AIs shows a positive rate of change, no significant growth trends were recorded.
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- 2022
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3. Impact of Simultaneous Nutrient Priming and Biopriming on Soybean Seed Quality and Health.
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Tamindžić, Gordana, Miljaković, Dragana, Ignjatov, Maja, Miladinović, Jegor, Đorđević, Vuk, Milošević, Dragana, Jovičić, Dušica, Vlajić, Slobodan, Budakov, Dragana, and Grahovac, Mila
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SUSTAINABLE agriculture ,FIELD crops ,BACILLUS megaterium ,SEED quality ,SEED viability ,GERMINATION - Abstract
In soybean production, numerous strategies are utilized to enhance seed quality and mitigate the effects of biotic and abiotic stressors. Zn-based nutrient priming has been shown to be effective for field crops, and biopriming is a strategy that is becoming increasingly important for sustainable agriculture. On the other hand, there is a lack of information about the effect of comprehensive nutrient priming and biopriming techniques on soybean seed quality and viability and seed health. This study was performed to assess the benefits of nutrient priming with Zn, biopriming with Bacillus megaterium and Bradyrhizobium japonicum (single and co-inoculation), and combination of nutrient priming and biopriming on the seed quality and viability, as well as seed infection caused by Alternaria spp. and Fusarium spp. Three different laboratory tests were employed: germination test, accelerated aging test, and seed health test. The results revealed that all tested priming treatments have a beneficial effect on seed germination, initial plant growth, and reduction of seed infection in normal and aged seeds. Additionally, comprehensive priming with Zn, Bacillus megaterium, and Bradyrhizobium japonicum reduced the occurrence of Alternaria spp. (−84% and −75%) and Fusarium spp. (−91% and −88%) on soybean seeds in the germination and accelerated aging tests, respectively, as compared to the control, which proved to be the most effective treatment in both optimal and stressful conditions. [ABSTRACT FROM AUTHOR]
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- 2024
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4. First Report of Brown Spot on Stored Apple Fruits Caused by Stemphylium vesicarium in Serbia
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Petreš, Mladen, primary, Loc, Marta, additional, Budakov, Dragana, additional, Dudaš, Tatjana, additional, Stojšin, Vera, additional, Petreš, Aleksandra Stankov, additional, and Grahovac, Mila, additional
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- 2024
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5. First Report of Brown Spot on Stored Apple Fruits Caused by Stemphylium vesicarium in Serbia
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Petreš, Mladen, Petreš, Mladen, Loc, Marta, Budakov, Dragana, Dudaš, Tatjana, Stojšin, Vera, Stankov Petreš, Aleksandra, Grahovac, Mila, Petreš, Mladen, Petreš, Mladen, Loc, Marta, Budakov, Dragana, Dudaš, Tatjana, Stojšin, Vera, Stankov Petreš, Aleksandra, and Grahovac, Mila
- Abstract
Apple is one of the most economically important fruit crops worldwide, and postharvest fungal diseases can cause significant losses during storage (Petreˇs et al. 2020). Apple fruits (cv. Fuji) with necrosis symptoms were collected during the fall of 2022 from an ultra-low-oxygen cold storage facility in Titel, Serbia. The fruits originated from the apple orchards in Titel (45°12947.10N, 20°15923.60E). The pathogens were isolated from collected fruit samples using standard phytopathological techniques. Fruits were surface-sterilized, rinsed with sterile water, and aseptically cut in half, and small fragments collected from the border of healthy and diseased tissue were placed on potato dextrose agar (PDA) medium in Petri dishes and incubated at 25 ± 1°C in the dark for 7 days. The obtained 11 isolates were identified to the genus level as Alternaria (incidence, 46%), Penicillium (36%), Fusarium (9%), and Stemphylium (9%) based on their morphological characteristics. Pathogenicity of all the isolates was confirmed on apple fruits of cultivars Fuji and Golden Delicious. The fruits were surface-sterilized, sprayed with 5 ml of conidial suspension (1 × 105 conidia/ml), and incubated at room temperature for 21 days. Symptoms developed on inoculated fruits were the same as symptoms observed on apple fruit samples collected from cold storage.
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- 2024
6. Assessment of Various Nanoprimings for Boosting Pea Germination and Early Growth in Both Optimal and Drought-Stressed Environments
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Tamindžić, Gordana, Tamindžić, Gordana, Azizbekian, Sergei, Miljaković, Dragana, Ignjatov, Maja, Nikolić, Zorica, Budakov, Dragana, Vasiljević, Sanja, Grahovac, Mila, Tamindžić, Gordana, Tamindžić, Gordana, Azizbekian, Sergei, Miljaković, Dragana, Ignjatov, Maja, Nikolić, Zorica, Budakov, Dragana, Vasiljević, Sanja, and Grahovac, Mila
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One of the main climate change-related variables limiting agricultural productivity that ultimately leads to food insecurity appears to be drought. With the use of a recently discovered nanopriming technology, seeds can endure various abiotic challenges. To improve seed quality and initial growth of 8-day-old field pea seedlings (cv. NS Junior) under optimal and artificial drought (PEG-induced) laboratory conditions, this study aimed to assess the efficacy of priming with three different nanomaterials: Nanoplant Ultra (Co, Mn, Cu, Fe, Zn, Mo, and Se), Nanoplant Ca-Si (Ca, Si, B, and Fe), and Nanoplant Sulfur (S). The findings indicate that nanopriming seed treatments have a positive impact on seed quality indicators, early plant growth, and drought resilience in field pea plants established in both optimal and drought-stressed conditions. Nevertheless, all treatments showed a positive effect, but their modes of action varied. Nanoplant Ultra proved to be the most effective under optimal conditions, whereas Nanoplant Ca-Si and Nanoplant Sulfur were the most efficient under drought stress. After a field evaluation, the examined comprehensive nanomaterials may be utilized as priming agents for pea seed priming to boost seed germination, initial plant growth, and crop productivity under various environmental conditions.
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- 2024
7. The Effect of Cultivation Conditions on Antifungal and Maize Seed Germination Activity of Bacillus-Based Biocontrol Agent
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Vlajkov, Vanja, primary, Pajčin, Ivana, additional, Loc, Marta, additional, Budakov, Dragana, additional, Dodić, Jelena, additional, Grahovac, Mila, additional, and Grahovac, Jovana, additional
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- 2022
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8. Genetic Diversity of Pectobacterium spp. on Potato in Serbia
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Loc, Marta, primary, Milošević, Dragana, additional, Ivanović, Žarko, additional, Ignjatov, Maja, additional, Budakov, Dragana, additional, Grahovac, Jovana, additional, and Grahovac, Mila, additional
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- 2022
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9. Ectomycorrhizal Fungi Modulate Biochemical Response against Powdery Mildew Disease in Quercus robur L.
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Kebert, Marko, primary, Kostić, Saša, additional, Zlatković, Milica, additional, Stojnic, Srdjan, additional, Čapelja, Eleonora, additional, Zorić, Martina, additional, Kiprovski, Biljana, additional, Budakov, Dragana, additional, and Orlović, Saša, additional
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- 2022
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10. EKONOMSKI ZNAČAJNI VIRUSI PAPRIKE UTVRĐENI NA PODRUČJU REPUBLIKE SRPSKE.
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Babić, Gordana, Trkulja, Vojislav, Budakov, Dragana, Iličić, Renata, Mališević, Rade, and Bagi, Ferenc
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- 2023
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11. Medium for the Production of Bacillus-Based Biocontrol Agent Effective against Aflatoxigenic Aspergillus flavus: Dual Approach for Modelling and Optimization
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Vlajkov, Vanja, primary, Anđelić, Stefan, additional, Pajčin, Ivana, additional, Grahovac, Mila, additional, Budakov, Dragana, additional, Jokić, Aleksandar, additional, and Grahovac, Jovana, additional
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- 2022
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12. Uticaj etarskih ulja i hidrolata etarskih ulja na razvoj prouzrokovača gorke truleži jabuke
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Petreš, Mladen, Petreš, Mladen, Aćimović, Milica, Loc, Marta, Dudaš, Tatjana, Budakov, Dragana, Stojšin, Vera, Grahovac, Mila, Petreš, Mladen, Petreš, Mladen, Aćimović, Milica, Loc, Marta, Dudaš, Tatjana, Budakov, Dragana, Stojšin, Vera, and Grahovac, Mila
- Abstract
Vrste roda Colletotrichum, prouzrokovači gorke truleži jabuke, predstavljaju jedne od najznačajnijih prouzrokovača truleži plodova jabuke u skladištu. Zbog dominantne zastupljenosti u odnosu na druge vrste ovog roda posebno su značajne vrste C. acutatum i C. gloeosporioides. S obzirom da upotreba fungicida nakon berbe i tokom skladištenja nije dozvoljena zbog njihovih nepovoljnih ekotoksikoloških svojstava, a plodovi ostaju nezaštićeni tokom skladištenja, neophodno je definisanje alternativnih rešenja. Prethodna istraživanja su dokazala značajan antifungalni potencijal etarskih ulja i njihovih hidrolata (sporednih proizvoda koji se dobijaju pri proizvodnji etarskih ulja), te mogu predstavljati adekvatnu zamenu fungicidima. U ogledu je ispitan uticaj etarskih ulja čubra (Satureja hortensis), bosiljka (Ocimum basilicum), muskatne žalfije (Salvia sclarea) i divljeg origana (Origanum vulgare), kao i hidrolata etarskih ulja čubra, bosiljka i muskatne žalfije na izolate KA7 (C. acutatum) i MRMCD6 (C. gloeosporioides).
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- 2022
13. Etarska ulja u suzbijanju prouzrokovača vlažne truleži krtola krompira iz roda Pectobacterium
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Loc, Marta, Loc, Marta, Grahovac, Mila, Aćimović, Milica, Petreš, Mladen, Budakov, Dragana, Dudaš, Tatjana, Stojšin, Vera, Milošević, Dragana, Loc, Marta, Loc, Marta, Grahovac, Mila, Aćimović, Milica, Petreš, Mladen, Budakov, Dragana, Dudaš, Tatjana, Stojšin, Vera, and Milošević, Dragana
- Abstract
Vrste roda Pectobacterium su proteklih godina postale jedne od deset najproučavanijih fitopatogenih bakterija. Pažnju zavređuju zbog sve učestalije pojave novih vrsta, ekstremnoj adaptivnosti različitim domaćinima i uslovima životne sredine. Intenzivna trgovinska razmena dovodi do povećanja mogućnosti dospevanja novih vrsta na udaljena područja proizvodnje krompira, uzimajući u obzir da je glavni izvor inokuluma zaražen vegetativni materijal namenjen za sadnju. Štete uzrokovane vrstama Pectobacterium dosežu velike razmere, s obzirom na to da svoju aktivnost nastavljaju i po završetku vegetacije – prilikom transporta, prerade i u uslovima skladišta. Suzbijanje ovih patogena postaje ekstremno zahtevno uzimajući u obzir opšti nedostatak baktericida, te su mnogobrojni napori učinjeni sa ciljem iznalaženja načina suzbijanja prouzrokovača vlažne truleži krtola u uslovima skladišta. Mnoga istraživanja ukazuju na visoku efikasnost biljnih ekstrakata i etarskih ulja, kao moćnih agenasa zaštite. U cilju utvrđivanja efekata etarskih ulja (EU) bosiljka (Ocimum basilicum), čubra (Satureja hortensis), divljeg origana (Origanum vulgare), vreska (Calluna vulgaris) i timjana (Thymus vulgaris) na vrste P. carotovorum (Pc), P. brasiliense (Pb) i P. punjabense (Pp), sproveden je ogled in planta, na krtolama krompira sorte Lady Claire, jedne od najosetljivijih sorti na Pectobacterium spp.
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- 2022
14. Osetljivost Macrophomina phaseolina prema Bacillus sp. i Trichoderma harzianum in vitro
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Stankov Petreš, Aleksandra, Stankov Petreš, Aleksandra, Budakov, Dragana, Stojšin, Vera, Nagl, Nevena, Grahovac, Mila, Ćurčić, Živko, Stankov Petreš, Aleksandra, Stankov Petreš, Aleksandra, Budakov, Dragana, Stojšin, Vera, Nagl, Nevena, Grahovac, Mila, and Ćurčić, Živko
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Primena fungicida u suzbijanju zemljišnih patogena ne predstavlja efikasnu i ekonomski opravdanu meru. Takođe, oni mogu da naruše balans korisnih mikroorganizama u zemljištu, dok njihove rezidue utiču na zagađenje životne sredine. U nastojanju da se smanji upotreba sintetičkih fungicida, primena bioloških agenasa može imati važnu ulogu u „eco-frendly“ poljoprivrednim sistemima. Biološki agensi kao što su vrste iz rodova Bacillus i Trichoderma se najčešće upotrebljavaju u biološkoj borbi protiv zemljišnih patogena. Cilj ovog istraživanja je bio da se ispita antagonistički uticaj Bacillus sp. i Trichoderma spp. na izolate Macrophomina phaseolina (ŠR55(3)/09 i ŠR23/11) u in vitro uslovima. Za ispitivanje je korišten Bacillus sp., 74 koji je komercijalno dostupan kao mikrobiološko đubrivo pod nazivom Bacillomix® Original (Bacillomix Co., Srbija). Metodom inkorporacije komercijalne suspenzije mikrobiološkog đubriva u KDA hranljivu podlogu ispitane su koncentracije preparata od 1, 2, 5 i 10%. U cilju ispitivanja osetljivosti M. phaseolina prema Trichoderma spp. korišteno je 13 izolata koji su predhodno identifikovani kao Trichoderma harzianum. Test dvojne kulture (TDK) je korišten kako bi utvrdili antagonistički uticaj ispitivanih izolata.
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- 2022
15. Ectomycorrhizal fungi modulate biochemical response against powdery mildew disease in Quercus robur L.
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Kebert, Marko, Kebert, Marko, Kostić, Saša, Zlatković, Milica, Stojnić, Srđan, Čapelja, Eleonora, Zorić, Martina, Kiprovski, Biljana, Budakov, Dragana, Orlović, Saša, Kebert, Marko, Kebert, Marko, Kostić, Saša, Zlatković, Milica, Stojnić, Srđan, Čapelja, Eleonora, Zorić, Martina, Kiprovski, Biljana, Budakov, Dragana, and Orlović, Saša
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In light of climate change, pedunculate oak (Q. robur L.) was marked as the most threatened European tree species. Pedunculate oak is particularly jeopardized by powdery mildew disease caused by Erysiphe alphitoides. We hypothesized that priming of this tree species with ectomycorrhizal fungi could mitigate biotic stress and produce bioprotective properties against the disease. In this study, we have compared oaks’ foliar physiological and biochemical responses upon infection with E. alphitoides in the presence and absence of ectomycorrhizal fungi (ECM). The main aim of this study was to inspect how ECM modulate an oak’s biochemical response to infection with E. alphitoides, particularly at the level of the accumulation of the main polyamines (putrescine, spermidine, and spermine), soluble osmolytes (proline and glycine betaine), and phenolics (total phenolic content, flavonoids, and condensed tannins). A polyamine quantification was performed after derivatization by using high-performance liquid chromatography (HLPC) coupled with fluorescent detection. Oak seedlings inoculated with ECM fungi exhibited significantly higher levels of putrescine, spermine, and proline compared to non-inoculated seedlings, indicating the priming properties of the ECM. E. alphitoides caused an increase in individual and total polyamine content and lipid peroxidation in oak leaves regardless of the effect of ECM, while causing a decrease in physiological and antioxidative parameters and water use efficiency (WUE). Common biochemical parameters may contribute to understanding the underpinning plant defense mechanisms in three-way interactions among plants and pathogenic and ectomycorrhizal fungi and can be used as reliable adaptability descriptors in the context of climate change.
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- 2022
16. Essential oils and hydrolates in control of plant pathogens
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Grahovac, Mila, Grahovac, Mila, Aćimović, Milica, Budakov, Dragana, Stojšin, Vera, Loc, Marta, Petreš, Mladen, Dudaš, Tatjana, Grahovac, Mila, Grahovac, Mila, Aćimović, Milica, Budakov, Dragana, Stojšin, Vera, Loc, Marta, Petreš, Mladen, and Dudaš, Tatjana
- Abstract
Under conditions of intensive plant production and intensive international trade with plant material, coupled with climate changes, plant pathogens became one of the major limiting factors in food and feed production. Besides these extremely favorable conditions for reproduction and dissemination of plant pathogens, resistance development to available control tools and ecotoxicological properties of these tools show that plant pathogen control has never been more challenging. Development of alternative, eco-friendly control tools against phytopathogens, became a necessity of contemporary agriculture. Essential oils are mixtures of complex volatile compounds. They are synthesized through secondary metabolic pathways in many plant species. Essential oils are normally extracted by hydro-distillation or steam distillation of plant tissues. Hydrolates are co-products of the distillation formed in significantly higher quantities than the target substance. Hydrolate represents aromatic water containing approximately 0.1% essential oil compounds.
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- 2022
17. First report of Pectobacterium punjabense causing potato soft rot and blackleg in Serbia
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Loc, Marta, Loc, Marta, Milošević, Dragana, Ignjatov, Maja, Ivanović, Žarko, Budakov, Dragana, Grahovac, Jovana, Vlajkov, Vanja, Pajčin, Ivana, Grahovac, Mila, Loc, Marta, Loc, Marta, Milošević, Dragana, Ignjatov, Maja, Ivanović, Žarko, Budakov, Dragana, Grahovac, Jovana, Vlajkov, Vanja, Pajčin, Ivana, and Grahovac, Mila
- Abstract
Soft rot and blackleg are common diseases of potato (Solanum tuberosum) in Serbia. Opportunistic pectinolytic plant pathogens in the genus Pectobacterium cause soft rot and wilt diseases by cell wall degradation. They cause major economic losses in potato crops worldwide and are among the top 10 plant pathogenic bacteria (Mansfield et al. 2012). Potato plants (‘VR808’) with symptoms of wilting, slow growth, stem blackening, and tuber softening were collected from a commercial field in Zobnatica, Serbia, in July 2019 and analyzed. All symptoms occurred in the same field, and ~5% of plants were symptomatic. Isolation was performed from 10 randomly chosen symptomatic plants and tubers. Plant tissue was surface disinfected, and 1-cm sections from the margins of lesions were macerated in sterile distilled water (SDW) for 25 min and streaked on nutrient agar. After 48 h of incubation at 26C, predominant shiny, cream-colored, round colonies were obtained from all samples. Three representative isolates (MMZKVR1, MMZCVR2, and MMZKVR3) from independent samples were selected randomly for biochemical and pathogenicity tests. Isolates were gram-negative, nonfluorescent facultative anaerobes exhibiting pectinolytic activity on potato tuber slices and hypersensitive response on tobacco leaves. They expressed catalase activity but did not express oxidase or acid phosphatase activity or produce indole.
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- 2022
18. The causal agent of apple scab (Venturia inaequalis (Cooke) Winter)
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Petreš, Mladen, primary, Grahovac, Mila, additional, Budakov, Dragana, additional, Stojšin, Vera, additional, Loc, Marta, additional, Dudaš, Tatjana, additional, and Došen, Nataša, additional
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- 2022
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19. Podosphaera leucotricha (Ellis et Everhart) Salmon: Powdery mildew of apple
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Dudaš, Tatjana, primary, Vukotić, Jelena, additional, Loc, Marta, additional, Petreš, Mladen, additional, Budakov, Dragana, additional, Grahovac, Mila, additional, and Stojšin, Vera, additional
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- 2022
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20. Penicillium: Species causing blue mold on stored apple fruits
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Dudaš, Tatjana, primary, Todić, Aleksandra, additional, Budakov, Dragana, additional, Grahovac, Mila, additional, Loc, Marta, additional, and Stojšin, Vera, additional
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- 2022
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21. ANTIMICROBIAL ACTIVITY OF THE Asclepias syriaca L. ROOT EXTRACT.
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Popov, Milena, Grahovac, Mila, Loc, Marta, Prvulović, Dejan, Budakov, Dragana, Konstantinović, Bojan, Samardžić, Nataša, and Stojanović, Tijana
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ALTERNARIA alternata ,PHYTOPATHOGENIC fungi ,ANTI-infective agents ,DISEASE resistance of plants ,SEED production (Botany) ,PLANT development - Abstract
Asclepias syriaca L. is an invasive plant in Serbia which threatens the biodiversity and ecosystem functionality with its high production of wind-dispersed seeds and the rapid proliferation by the lateral rhizomes. The mechanism that allows its competitiveness is allelopathy - the release of the chemical compounds identified in the roots and leaves which have negative effects on the other plants. These allelochemicals not only affect the development of the neighboring plants, but also are a potential source of the antioxidant and antimicrobial compounds, which increase the immunity of the plant itself, protecting it from the pests and the pathogens. The present study aimed to screen the antifungal activity of the A. syriaca water and methanol extracts. In vitro antimicrobial activity was analyzed by the radial growth assays against the three phytopathogenic fungi isolates: Alternaria alternata, Fusarium avenaceum and Discula platani. The results were processed by factorial ANOVA and the statistically significant differences were determined by Duncan's multiple range test using the software STATISTICA 13.5. The obtained results suggest that the A. syriaca water extract has a significant fungistatic and potential fungicidal effect towards the tested phytopathogenic fungi and thus can be considered as a potential tool for their biological control. [ABSTRACT FROM AUTHOR]
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- 2023
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22. Podosphaera leucotricha (ELLIS ET EVERHART) SALMON - PROUZROKOVAČ PEPELNICE JABUKE.
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Dudaš, Tatjana, Vukotić, Jelena, Loc, Marta, Petreš, Mladen, Budakov, Dragana, Grahovac, Mila, and Stojšin, Vera
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- 2022
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23. PROUZROKOVAČ ČAĐAVE PEGAVOSTI LISTA I KRASTAVOSTI PLODA JABUKE (VENTURIA INAEQUALIS (COOKE) WINTER).
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Petreš, Mladen, Grahovac, Mila, Budakov, Dragana, Stojšin, Vera, Loc, Marta, Dudaš, Tatjana, and Došen, Nataša
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- 2022
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24. Trichoderma harzianumas a potential biological agent in control of the charcoal root rot of sugar beet
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Stankov Petreš, Aleksandra, Stojšin, Vera, Nagl, Nevena, Petreš, Mladen, Grahovac, Mila, Ćurčić, Živko, and Budakov, Dragana
- Abstract
Sugar beet is one of the most profitable crops in Serbia. The charcoal root rot is an emerging disease of sugar beet that significantly reduces yield and affects the economic viability of production. The objectives of this research were to determine the antagonist activity of selected Trichoderma harzianumisolates (T1 to T13) on two Macrophomina phaseolinaisolates in vitro using a dual culture test and a volatile compounds test, as well as in vivo. The efficacy of these treatments was compared to two commercial biopesticides (Bacillomix® Original and Trifender Pro®) and one synthetic fungicide (Funomil 700 WG®). The strongest inhibition in the dual culture test was observed in the treatment with T. harzianumT2 against both tested M. phaseolinaisolates. Also, the volatile compounds produced by isolates T2 and T12 exhibited the strongest inhibitory effect on M. phaseolinaisolates (from 48.22 to 62.75%). Differences in efficacy were found not only between tested Trichodermaisolates, but also between M. phaseolinaisolates used for inoculation which indicates different susceptibility of pathogen population to bioagent. The in vivo test confirmed the strong antagonistic effect of the T. harzianumT2 and T12 isolates. Bacillomix® Original, Trifender Pro® and Funomil® 700 WG did not express satisfactory effects in pathogen control.
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- 2023
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25. Distribution, Genetic Diversity and Biocontrol of Aflatoxigenic Aspergillus flavus in Serbian Maize Fields.
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Vlajkov, Vanja, Grahovac, Mila, Budakov, Dragana, Loc, Marta, Pajčin, Ivana, Milić, Dragan, Novaković, Tihomir, and Grahovac, Jovana
- Subjects
GENETIC variation ,ASPERGILLUS flavus ,CORN ,AFLATOXINS ,HIGH performance liquid chromatography ,ENZYME-linked immunosorbent assay ,COMMON bean - Abstract
Maize is one of the leading export products in the Republic of Serbia. As a country where economic development depends on agriculture, maize production plays a critical role as a crop of strategic importance. Potential aflatoxin contamination of maize poses a risk to food and feed safety and tremendous economic losses. No aflatoxin contamination of maize samples harvested in 2019 and 2020 in different localities in the Republic of Serbia was detected by the Enzyme-Linked Immunosorbent Assay (ELISA) test and High-Performance Liquid Chromatography (HPLC) method. On the other hand, the Cluster Amplification Patterns (CAP) analyses of the isolated Aspergillus flavus strains from 2019 maize samples confirmed the presence of key biosynthesis genes responsible for aflatoxin production. Artificial inoculation and subsequent HPLC analysis of the inoculated maize samples confirmed the high capacity of the A. flavus strains for aflatoxin production, pointing to a high risk of contamination under favorable conditions. Prevention of aflatoxin contamination is primarily based on A. flavus control, where biocontrol agents play a significant role as sustainable disease management tools. In this study, antagonistic activity screening of the novel strains belonging to the Bacillus genus indicated superior suppression of A. flavus strains by two Bacillus strains isolated from the rhizosphere of Phaseolus vulgaris. [ABSTRACT FROM AUTHOR]
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- 2021
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26. INFLUENCE OF DIFFERENT REDUCING AGENTS ON GRAŠEVINA WHITE WINE AROMATIC PROFILE
- Author
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Atlija, Jure, Budžaki, Anja, Oreški, Andrijana, Jagatić Korenika, Ana-Marija, Budakov, Dragana, and Ćupina, Branko
- Subjects
sulfur dioxide, glutathione, antioxidants, inactive yeast - Abstract
Graševina is the most planted grape variety in Republic of Croatia with great quality potential. The quality of grapes, and thus of wine, largely depends on the conditions in which they are grown. If they are grown in appropriate conditions, they can give high quality wines. However, like all white wines, Graševina wine needs protection from oxidation and discoloration, for which sulfur dioxide (SO2) is most often used. As the most commonly used preservative in wine due to the antioxidant, antimicrobial and coagulation properties, its use is almost indispensable, although it can cause allergic reactions in a part of sensitive population. Therefore, consumers are increasingly reaching for wines produced with alternative procedures or agents that prevent oxidation and discoloration, such as glutathione. In this study, the quality and chemical composition of Graševina wines, produced with SO2 and alternative agents, were compared. The analysis showed significantly higher concentrations of total fatty acids, esters and higher alcohols in Graševina wine produced with SO2, together with more quality sensory properties.
- Published
- 2023
27. Assessment of Various Nanoprimings for Boosting Pea Germination and Early Growth in Both Optimal and Drought-Stressed Environments.
- Author
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Tamindžić G, Azizbekian S, Miljaković D, Ignjatov M, Nikolić Z, Budakov D, Vasiljević S, and Grahovac M
- Abstract
One of the main climate change-related variables limiting agricultural productivity that ultimately leads to food insecurity appears to be drought. With the use of a recently discovered nanopriming technology, seeds can endure various abiotic challenges. To improve seed quality and initial growth of 8-day-old field pea seedlings (cv. NS Junior) under optimal and artificial drought (PEG-induced) laboratory conditions, this study aimed to assess the efficacy of priming with three different nanomaterials: Nanoplant Ultra (Co, Mn, Cu, Fe, Zn, Mo, and Se), Nanoplant Ca-Si (Ca, Si, B, and Fe), and Nanoplant Sulfur (S). The findings indicate that nanopriming seed treatments have a positive impact on seed quality indicators, early plant growth, and drought resilience in field pea plants established in both optimal and drought-stressed conditions. Nevertheless, all treatments showed a positive effect, but their modes of action varied. Nanoplant Ultra proved to be the most effective under optimal conditions, whereas Nanoplant Ca-Si and Nanoplant Sulfur were the most efficient under drought stress. After a field evaluation, the examined comprehensive nanomaterials may be utilized as priming agents for pea seed priming to boost seed germination, initial plant growth, and crop productivity under various environmental conditions.
- Published
- 2024
- Full Text
- View/download PDF
28. First Report of Collar and Root Rot of Lettuce Caused by Plectosphaerella cucumerina in Serbia.
- Author
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Mihajlović M, Hrustić J, Vučurović A, Grahovac M, Budakov D, Dudaš T, and Pešić B
- Abstract
In March 2021, unusual plant stuning, collar, and wet root rot of lettuce ( Lactuca sativa L.) during the rosette stage was observed in two commercial fields in Serbia (44°58'N, 20°32'E; 44°45'N, 20°43'E). Disease incidence in the fields (≈ 0.9 ha each) was approximately 15 and 20%, respectively. Initial above-ground symptoms were yellowing and wilting of leaves, while below-ground symptoms were collar, wet root rot, and lesions becoming necrotic. Eventually, whole plants wilted, collapsed, and died. A total of 35 symptomatic plants were collected from the fields, and diseased tissues were cut into small pieces, surface sterilized, and plated on potato dextrose agar (PDA). Isolation resulted in 20 morphologically uniform monoconidial isolates. The isolates formed white to creamy colonies, gradually becoming salmon pink, slimy, or moist in appearance, with sparse aerial mycelia. Numerous hyphal coils with conidiophores and hyaline, smooth-surfaced, ellipsoid to ovoid, septate or aseptate conidia were formed (4.5 to 10.1×1.2 to 3.7 μm (n = 100)). To confirm the species identity, the internal transcribed spacer (ITS) region and the D1/D2 region of a selected representative isolate 13-3-c were amplified and sequenced by using primer pairs ITS1/ITS4 (White et al. 1990) and N1/N2 (O'Donnell and Gray 1995), respectively. The sequences were deposited in GenBank (ITS: OR880564 and D1/D2: OR880567). Sequence analysis revealed 100% nucleotide identity with P. cucumerina isolates from different countries deposited in the NCBI GenBank, including isolate MH860704 (Vu et al. 2019) (ITS region) and isolate KY662256 (Su et al. 2017) (D1/D2 region). Neighbor-joining analysis was conducted based on the combined ITS and D1/D2 regions, and the tree was constructed with the substitution models (1,000 bootstrap). The combined phylogeny confirmed that the sequences shared a common clade with P. cucumerina . Hence, morphological, microscopic, and molecular characterization confirmed the pathogen as P. cucumerina (Palm et al., 1995; Carlucci et al., 2012). In a pathogenicity assay, 10 isolates were tested. Five 30-day-old lettuce plants (cv. Majska Kraljica) per isolate were root-dipped in the conidial suspensions (1×105 conidia/ml). The 10 inoculated plants were transplanted into 1 L pots containing sterile substrate (Floragard, Germany). Plants treated with sterile distilled water were used as controls. Plants were maintained in a greenhouse at 25 to 28°C under a 12-hour photoperiod (Cai et al., 2021). Four weeks after inoculation, stunting, chlorosis, and wilting of plants were observed, while collars and roots exhibited typical decaying symptoms. No symptoms were observed on the control plants. The pathogen was reisolated from symptomatic tissue as previously described. Koch's postulates were completed by confirming the identity of reisolates based on morphological features. To our knowledge, this is the first report of P. cucumerina on lettuce or any other crop in Serbia. P. cucumerina is already known as a pathogen of lettuce and other hosts grown in many countries worldwide, as well as in some European countries (Belgium, England, Italy, the Netherlands, and Switzerland) (Zhang et al. 2019). This emerging pathogen may cause significant economic losses in lettuce production in Serbia and in the entire Balkan region. Our results may help to develop effective management strategies based on accurate and timely identification and regular pathogen monitoring.
- Published
- 2024
- Full Text
- View/download PDF
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