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Your search keyword '"Zuo, Xiaolei"' showing total 583 results
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583 results on '"Zuo, Xiaolei"'

2. Twisted DNA origami-based chiral monolayers for spin filtering

Author

Wang, Haozhi, Yin, Fangfei, Li, Linyun, Li, Mingqiang, Fang, Zheng, Sun, Chenyun, Li, Bochen, Shi, Jiye, Li, Jiang, Wang, Lihua, Song, Shiping, Zuo, Xiaolei, Liu, Xiaoguo, and Fan, Chunhai

Subjects
Physics - Chemical Physics
Abstract

DNA monolayers with inherent chirality play a pivotal role across various domains, including biosensors, DNA chips, and bioelectronics. Nonetheless, conventional DNA chiral monolayers, typically constructed from single-stranded DNA (ssDNA) or double-stranded DNA (dsDNA), often lack structural orderliness and design flexibility at the interface. Structural DNA nanotechnology emerges as a promising solution to tackle these challenges. In this study, we present a strategy for crafting highly adaptable twisted DNA origami-based chiral monolayers. These structures exhibit distinct interfacial assembly characteristics and effectively mitigate the structural disorder of dsDNA monolayers, which is constrained by a limited persistence length of ~50 nm of dsDNA. We highlight the spin-filtering capabilities of four representative DNA origami-based chiral monolayers, demonstrating a maximal one-order-of-magnitude increase in spin-filtering efficiency per unit area compared to conventional dsDNA chiral monolayers. Intriguingly, our findings reveal that the higher-order, tertiary, chiral structure of twisted DNA origami further enhances the spin-filtering efficiency. This work paves the way for the rational design of DNA chiral monolayers.

Published
2023

9. Encoding quantized fluorescence states with fractal DNA frameworks.

Author

Li, Jiang, Dai, Jiangbing, Jiang, Shuoxing, Xie, Mo, Zhai, Tingting, Guo, Linjie, Cao, Shuting, Xing, Shu, Qu, Zhibei, Zhao, Yan, Wang, Fei, Yang, Yang, Liu, Lei, Zuo, Xiaolei, Wang, Lihua, Yan, Hao, and Fan, Chunhai

Subjects
Hela Cells, Humans, DNA, Oligonucleotides, Fluorescent Dyes, Microscopy, Confocal, Microscopy, Atomic Force, Fluorescence Resonance Energy Transfer, Algorithms, Fractals, Fluorescence, Nanostructures, HeLa Cells, Microscopy, Atomic Force, Confocal
Abstract

Signal amplification in biological systems is achieved by cooperatively recruiting multiple copies of regulatory biomolecules. Nevertheless, the multiplexing capability of artificial fluorescent amplifiers is limited due to the size limit and lack of modularity. Here, we develop Cayley tree-like fractal DNA frameworks to topologically encode the fluorescence states for multiplexed detection of low-abundance targets. Taking advantage of the self-similar topology of Cayley tree, we use only 16 DNA strands to construct n-node (n = 53) structures of up to 5 megadalton. The high level of degeneracy allows encoding 36 colours with 7 nodes by site-specifically anchoring of distinct fluorophores onto a structure. The fractal topology minimises fluorescence crosstalk and allows quantitative decoding of quantized fluorescence states. We demonstrate a spectrum of rigid-yet-flexible super-multiplex structures for encoded fluorescence detection of single-molecule recognition events and multiplexed discrimination of living cells. Thus, the topological engineering approach enriches the toolbox for high-throughput cell imaging.

Published
2020

20. Nucleic Acids Analysis

Author

Zhao, Yongxi, Zuo, Xiaolei, Li, Qian, Chen, Feng, Chen, Yan-Ru, Deng, Jinqi, Han, Da, Hao, Changlong, Huang, Fujian, Huang, Yanyi, Ke, Guoliang, Kuang, Hua, Li, Fan, Li, Jiang, Li, Min, Li, Na, Lin, Zhenyu, Liu, Dingbin, Liu, Juewen, Liu, Libing, Liu, Xiaoguo, Lu, Chunhua, Luo, Fang, Mao, Xiuhai, Sun, Jiashu, Tang, Bo, Wang, Fei, Wang, Jianbin, Wang, Lihua, Wang, Shu, Wu, Lingling, Wu, Zai-Sheng, Xia, Fan, Xu, Chuanlai, Yang, Yang, Yuan, Bi-Feng, Yuan, Quan, Zhang, Chao, Zhu, Zhi, Yang, Chaoyong, Zhang, Xiao-Bing, Yang, Huanghao, Tan, Weihong, and Fan, Chunhai

Published
2021
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23. High-throughput DNA synthesis for data storage.

Author

Yu, Meng, Tang, Xiaohui, Li, Zhenhua, Wang, Weidong, Wang, Shaopeng, Li, Min, Yu, Qiuliyang, Xie, Sijia, Zuo, Xiaolei, and Chen, Chang

Subjects
DATA warehousing, DNA, DIGITAL technology, DNA sequencing, DNA synthesis
Abstract

With the explosion of digital world, the dramatically increasing data volume is expected to reach 175 ZB (1 ZB = 1012 GB) in 2025. Storing such huge global data would consume tons of resources. Fortunately, it has been found that the deoxyribonucleic acid (DNA) molecule is the most compact and durable information storage medium in the world so far. Its high coding density and long-term preservation properties make itself one of the best data storage carriers for the future. High-throughput DNA synthesis is a key technology for "DNA data storage", which encodes binary data stream (0/1) into quaternary long DNA sequences consisting of four bases (A/G/C/T). In this review, the workflow of DNA data storage and the basic methods of artificial DNA synthesis technology are outlined first. Then, the technical characteristics of different synthesis methods and the state-of-the-art of representative commercial companies, with a primary focus on silicon chip microarray-based synthesis and novel enzymatic DNA synthesis are presented. Finally, the recent status of DNA storage and new opportunities for future development in the field of high-throughput, large-scale DNA synthesis technology are summarized. [ABSTRACT FROM AUTHOR]

Published
2024
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24. A Programmable DNAzyme for the Sensitive Detection of Nucleic Acids

Author

Shi, Chenzhi, primary, Yang, Donglei, additional, Ma, Xiaowei, additional, Pan, Li, additional, Shao, Yuanchuan, additional, Arya, Gaurav, additional, Ke, Yonggang, additional, Zhang, Chuan, additional, Wang, Fuan, additional, Zuo, Xiaolei, additional, Li, Min, additional, and Wang, Pengfei, additional

Published
2024
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28. Data Storage Using DNA

Author

Wang, Shaopeng, primary, Mao, Xiuhai, additional, Wang, Fei, additional, Zuo, Xiaolei, additional, and Fan, Chunhai, additional

Published
2023
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45. A Membrane Tension‐Responsive Mechanosensitive DNA Nanomachine

Author

Zheng, Haoran, primary, Li, Haidong, additional, Li, Mingqiang, additional, Zhai, Tingting, additional, Xie, Xiaodong, additional, Li, Cong, additional, Jing, Xinxin, additional, Liang, Chengpin, additional, Li, Qian, additional, Zuo, Xiaolei, additional, Li, Jiang, additional, Fan, Jiangli, additional, Shen, Jianlei, additional, Peng, Xiaojun, additional, and Fan, Chunhai, additional

Published
2023
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47. Twisted DNA Origami-Based Chiral Monolayers for Spin Filtering

Author

Wang, Haozhi, Yin, Fangfei, Li, Lingyun, Li, Mingqiang, Fang, Zheng, Sun, Chenyun, Li, Bochen, Shi, Jiye, Li, Jiang, Wang, Lihua, Song, Shiping, Zuo, Xiaolei, Liu, Xiaoguo, and Fan, Chunhai

Abstract

DNA monolayers with inherent chirality play a pivotal role across various domains including biosensors, DNA chips, and bioelectronics. Nonetheless, conventional DNA chiral monolayers, typically constructed from single-stranded DNA (ssDNA) or double-stranded DNA (dsDNA), often lack structural orderliness and design flexibility at the interface. Structural DNA nanotechnology has emerged as a promising solution to tackle these challenges. In this study, we present a strategy for crafting highly adaptable twisted DNA origami-based chiral monolayers. These structures exhibit distinct interfacial assembly characteristics and effectively mitigate the structural disorder of dsDNA monolayers, which is constrained by a limited persistence length of ∼50 nm of dsDNA. We highlight the spin-filtering capabilities of seven representative DNA origami-based chiral monolayers, demonstrating a maximal one-order-of-magnitude increase in spin-filtering efficiency per unit area compared with conventional dsDNA chiral monolayers. Intriguingly, our findings reveal that the higher-order tertiary chiral structure of twisted DNA origami further enhances the spin-filtering efficiency. This work paves the way for the rational design of DNA chiral monolayers.

Published
2024
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