71 results on '"Shunsuke, FUJII"'
Search Results
2. Application of Monoclonal Antibodies against Naturally Occurring Bioactive Ingredients
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Shunsuke Fujii, Takuhiro Uto, Hiroaki Hayashi, Waraporn Putalun, Seiichi Sakamoto, Hiroyuki Tanaka, and Yukihiro Shoyama
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monoclonal antibody ,eastern blotting ,knockout extractives ,pharmacological activity ,Kampo medicine ,Immunologic diseases. Allergy ,RC581-607 - Abstract
Monoclonal antibodies (Mabs) are widely used in a variety of fields, including protein identification, life sciences, medicine, and natural product chemistry. This review focuses on Mabs against naturally occurring active compounds. The preparation of Mabs against various active compounds began in the 1980s, and now there are fewer than 50 types. Eastern blotting, which was developed as an antibody staining method for low-molecular-weight compounds, is useful for its ability to visually represent specific components. In this method, a mixture of lower-molecular-weight compounds, particularly glycosides, are separated by thin-layer chromatography (TLC). The compounds are then transferred to a membrane by heating, followed by treatment with potassium periodate (KIO4) to open the sugar moiety of the glycoside on the membrane to form an aldehyde group. Proteins are then added to form Schiff base bonds to enable adsorption on the membrane. A Mab is bound to the glycoside moiety on the membrane and reacts with a secondary antibody to produce color. Double Eastern blotting, which enables the simultaneous coloration of two glycosides, can be used to evaluate quality and estimate pharmacological effects. An example of staining by Eastern blotting and a component search based on the results will also be presented. A Mab-associated affinity column is a method for isolating antigen molecules in a single step. However, the usefulness of the wash fractions that are not bound to the affinity column is unknown. Therefore, we designated the wash fraction the “knockout extract”. Comparing the nitric oxide (NO) production of a glycyrrhizin (GL)-knockout extract of licorice with a licorice extract revealed that the licorice extract is stronger. Therefore, the addition of GL to the GL-knockout extract of licorice increased NO production. This indicates that GL has synergic activity with the knockout extract. The GL-knockout extract of licorice inhibited high-glucose-induced epithelial–mesenchymal transition in NRK-52E cells, primarily by suppressing the Notch2 pathway. The real active constituent in licorice may be constituents other than GL, which is the causative agent of pseudohyperaldosteronism. This suggests that a GL-knockout extract of licorice may be useful for the treatment of diabetic nephritis.
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- 2024
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3. Infantile hepatic hemangioma and hepatic mesenchymal hamartoma in an infant associated with placental mesenchymal dysplasia: a case report
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Shunsuke Fujii, Kyoko Mochizuki, Hidehito Usui, Norihiko Kitagawa, Sayoko Umemoto, Mio Tanaka, Yukichi Tanaka, Masako Otani, Kumiko Nozawa, Kenji Kurosawa, Masayo Kagami, and Masato Shinkai
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Hepatic infantile hemangioma ,Hepatic mesenchymal hamartoma ,Placental mesenchymal dysplasia ,Propranolol ,Tumorectomy ,Surgery ,RD1-811 - Abstract
Abstract Background Although infantile hepatic hemangioma and hepatic mesenchymal hamartoma are relatively common in benign pediatric liver tumors, coexistence of the two tumors is rare. Placental mesenchymal dysplasia is also a rare disorder. We report the case of a baby girl born after a pregnancy complicated by placental mesenchymal dysplasia, who developed both infantile hepatic hemangioma and hepatic mesenchymal hamartoma. Case presentation The patient was born at 32 weeks and 5 days of gestation for impending placental abruption, weighing 1450 g. Liver tumors, composed of both hypervascular solid and large cystic lesions, were detected after birth and markedly increased to create abdominal distention within 9 months. Diagnostic imaging suspected the coexistence of infantile hepatic hemangioma and cystic hepatic mesenchymal hamartoma. Following propranolol therapy for infantile hepatic hemangioma and needle puncture of a large cyst, the cystic lesions and adjacent hypervascular lesions were partially resected via laparotomy. Pathological findings confirmed the coexistence of hepatic mesenchymal hamartoma and infantile hepatic hemangioma, which had no association with androgenetic/biparental mosaicism. The postoperative course was uneventful, and the tumor had not regrown after 3 years. Conclusions Although the coexistence of infantile hepatic hemangioma and hepatic mesenchymal hamartoma associated with placental mesenchymal dysplasia is extremely rare, the pathological and pathogenetic similarities between these disorders suggest that they could have derived from similar embryologic origins rather than being a mere coincidence. Further follow-up is required, with careful attention to the potential for malignant hepatic mesenchymal hamartoma transformation.
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- 2022
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4. Preoperative AaDO2 as an indicator for physiologic stabilization for repair in infants with congenital diaphragmatic hernia: A multicenter retrospective study
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Akiko Yokoi, Shunsuke Fujii, Akiyoshi Nomura, Masaya Yamoto, Satoko Ofuji, Masato Shinkai, and Naoto Urushihara
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Congenital diaphragmatic hernia ,Optimal timing of surgery ,Alveolar-arterial oxygen difference ,Oxygenation index ,Neonates ,Pediatrics ,RJ1-570 - Abstract
Background: Appropriate timing of surgery for neonates with congenital diaphragmatic hernia (CDH) generally has been considered after cardiopulmonary stabilization. However, few reports clearly showed the definition of physiologic stabilization for surgery, while many studies have addressed hours of life for timing of repair. The aim of this study is to elucidate an optimal respiratory condition for repair in CDH. Methods: A retrospective chart review was conducted of 81 isolated CDH patients who were born and treated in three children's hospitals during Jan. 2009 through Sep.2018. To find an optimal respiratory condition for repair, alveolar-arterial oxygen difference (AaDO2) and oxygenation index (OI) right before surgery was investigated, as well as prenatal prognostic factors (observed/expected lung-to-head ratio (o/e LHR) and liver up), patients’ demographics (sex, gestational weeks, birth weight, Apgar scores), and the 30-day survival. Multivariate logistic regression analysis adjusting for the antenatal severity combined with o/e LHR and liver position was performed. Results: Among 81patients, 75 patients underwent surgery. The data of AaDO2 and OI right before surgery were available in 73 patients. Among 73 patients, six died in total, and of those, two out of five with extracorporeal membrane oxygenation (ECMO) died. The 30-day mortality in the subset of the fourth quartile of AaDO2 (≥340 mmHg) was significantly higher (odds Ratio (OR) 9.23 95% confidence interval (CI) 1.18072.11 P = 0.03). While mortality of the fourth quartile of OI (≥8) was also higher, it was marginally significant (OR 6.52 95% CI 0.93–45.59 p = 0.06). Conclusions: AaDO2 less than 340 mmHg could be a useful stabilization index for safe surgery in neonates with CDH.
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- 2023
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5. Gallbladder cancer 20 years after surgery for duodenal atresia with biliary anomalies
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Shunsuke Fujii, Kouji Masumoto, Takato Sasaki, Fumiko Chiba, Takahiro Jimbo, and Yasuhisa Urita
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Duodenal atresia ,Gallbladder cancer ,Pancreaticobiliary maljunction ,Pediatrics ,RJ1-570 ,Surgery ,RD1-811 - Abstract
The prognosis for duodenal atresia (DA) is generally good if radical surgery is performed. However, some cases of DA may be complicated by morphological abnormalities in the pancreaticobiliary system, which may cause problems in the distant period. Here, we report a case of gallbladder cancer (GC) that developed more than 20 years after the initial DA operation. A female infant was admitted to our institution with an anorectal malformation after birth. She had Down syndrome, a ventricular septal defect, and DA. She underwent duodenoduodenostomy for DA, during which a Y-shaped biliary duct was found. The patient grew up well postoperatively, and she was followed up at our institution until the age of 12 years. At the age of 24, she was reintroduced to our institution for multiple hepatic tumors and was diagnosed with GC and multiple liver metastases. Simultaneously, a pancreaticobiliary maljunction (PBM) without a choledochal cyst was also identified. She received palliative care and died two months later. Although reports of combined DA and PBM are rare, the combination should not be overlooked, given the carcinogenic risk of PBM. DA with pancreaticobiliary anomalies requires attention to the possibility of PBM during the postoperative follow-up.
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- 2023
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6. Takotsubo cardiomyopathy in a toddler with pulmonary atresia and ventricular septal defect
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Kenichi Tetsuhara, Shota Muraji, Mamoru Muraoka, and Shunsuke Fujii
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Heart Defects, Congenital ,Heart Septal Defects, Ventricular ,Pulmonary Atresia ,Takotsubo Cardiomyopathy ,Child, Preschool ,Humans ,Correction ,General Medicine - Published
- 2024
7. Plastic foreign body in the pharynx can evade detection by computed tomography
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Naoya Sakamoto, Shunsuke Fujii, Kouji Masumoto, Akira Matsuoka, Satoshi Toumine, Tadahiko Hara, and Ken Shimada
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Pediatric ,Foreign body ingestion ,Pharynx ,Pediatrics ,RJ1-570 ,Surgery ,RD1-811 - Abstract
Foreign body ingestion in trachea is a common occurrence in children. However, pharyngeal foreign bodies are rare. Unlike the trachea, the lumen of the pharynx is not kept patent by tracheal cartilage. Therefore, it is difficult to distinguish the lumen of the pharynx from the surrounding tissues. In addition, tracheal foreign bodies are photographed in the lung window, so radiolucent objects may be seen, but the pharynx in the mediastinal window may sometimes appear to be invisible. If a pharyngeal foreign body is suspected, then it is important to obtain a CT scan of the neck and to also obtain a lung window image. However, the most important thing is not to rely on CT alone, but to also use endoscopy when necessary.
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- 2022
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8. The Association between the First Cry and Clinical Outcomes in CDH Neonates: A Retrospective Study
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Yuki Takeuchi, Akiyoshi Nomura, Masaya Yamoto, Satoko Ohfuji, Shunsuke Fujii, Seiji Yoshimoto, Toru Funakoshi, Masato Shinkai, Naoto Urushihara, and Akiko Yokoi
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congenital diaphragmatic hernia ,first cry ,fetal sedation ,survival ,Pediatrics ,RJ1-570 - Abstract
Congenital diaphragmatic hernia (CDH) is a life-threatening condition characterized by the herniation of abdominal organs into the thorax, resulting in hypoplastic lungs and pulmonary hypertension. The impact of the first cry, a crucial event for lung transition during birth, on CDH patients remains unclear. This study investigated the impact of the first cry during birth on CDH patient survival, along with other prognosis factors. A multi-institutional retrospective study assessed CDH patient characteristics and survival rates by analyzing factors including the first cry, disease severity, birth weight, Apgar scores, oxygenation index (OI) and surgical closure. Among the CDH patients in the study, a positive first cry was linked to 100% survival, regardless of disease severity (p < 0.001). Notably, the presence of a positive first cry did not significantly affect survival rates in patients with worse prognostic factors, such as low birth weight (
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- 2023
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9. Development of Microdrip Enzyme Device Using Carbon-Coated Porous Silica Spheres
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Shunsuke Fujii, Akiko Yoshida, Tracy T. Chuong, Yuka Minegishi, Kritin Pirabul, Zheng-Ze Pan, Yuta Nishina, Takashi Kyotani, Hirotomo Nishihara, Kouji Masumoto, Galen D. Stucky, and Tetsuji Itoh
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- 2023
10. Bioimprinting as a Receptor for Detection of Kwakhurin
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Seiichi Sakamoto, Kei Minami, Poomraphie Nuntawong, Gorawit Yusakul, Waraporn Putalun, Hiroyuki Tanaka, Shunsuke Fujii, and Satoshi Morimoto
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bioimprinting ,enzyme-linked bioimprinted-protein assay (ELBIA) ,enzyme-linked immunosorbent assay (ELISA) ,kwakhurin (Kwa) ,monoclonal antibody (MAb) ,ovalbumin (OVA) ,Microbiology ,QR1-502 - Abstract
Bioimprinting was performed against ovalbumin (OVA) to confer its binding cavities for kwakhurin (Kwa), an isoflavonoid, produced solely by Pueraria candollei var. mirifica (P. candollei). The characterization of bioimprinted-OVA (biOVA), evaluated by an enzyme-linked immunosorbent assay (ELISA), revealed that it functioned as a specific receptor for Kwa. Using biOVA, two systems, i.e., an indirect competitive ELISA (icELISA) and the even simpler and more rapid competitive enzyme-linked bioimprinted-protein assay (cELBIA), were developed as novel techniques for the quantitative analysis of Kwa in P. candollei and its related products. The two analysis methods were found to have limits of detection (LOD) of 4.0 and 2.5 µg/mL, respectively. The high reliability of the developed icELISA and cELBIA using biOVA was also demonstrated by various validation analyses. Subsequently, bioimprinting was performed using eight other proteins to investigate them as candidate scaffolds for the generation of binding cavities for Kwa. Interestingly, two bioimprinted-IgG monoclonal antibodies (biMAbs) recognized Kwa, but their original binding affinity to hapten was lost. That is, the MAbs obtained a new binding ability to Kwa in exchange for their original binding affinity, raising the possibility that biMAb could be alternatively used as a probe for the quantitative analysis of Kwa as well as biOVA. This is the first report of small molecules recognition by MAbs used as proteins for bioimprinting.
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- 2022
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11. Nutrition support therapy for refractory elbow subcutaneous abscess in a child with atopic dermatitis and malnutrition:A case report
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Shunsuke Fujii, Kouji Masumoto, Toko Shinkai, Hinako Horiguchi, Yasunari Tanaka, Yudai Goto, Takato Sasaki, Fumiko Chiba, Takahiro Jimbo, and Yasuhisa Urita
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Microbiology (medical) ,Immunology ,Immunology and Allergy - Published
- 2023
12. Effects of Heating Methods and Water-Cooling Time on Spinach Quality Assessed Using Different Large-scale Cooking Equipment
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Ryoko, KIKUCHI, Natsuki, YOSHINAGA, Shunsuke, FUJII, Tomoaki, NODA, and Takako, KOGA
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加熱法 ,water-cooling t ime ,大量調理機器 ,large-scale cooking equipment ,水冷時間 ,heating methods - Abstract
前報に続き本研究では、スチコン、バリオおよび鍋の3種の大量調理機器を用いた加熱法および水冷時間がほうれん草の性状に与える影響について明らかにすることを目的とした。バリオ加熱は、加熱初期の温度上昇が早く、その後もパン底全面の温度を高温維持できるため、食材投入後も茹で水を高温保持でき、食材の品温も高温になりやすい。可溶性固形分は、大量調理機器間で比較すると、バリオ2分加熱および鍋2分加熱と比較し、スチコン3分加熱のほうが有意に高かった(p<0.05)。さらに加熱後の水さらしにより、可溶性固形分は低い値を示した。ビタミンCの残存率は、水を介して加熱するバリオ2分加熱および鍋2分加熱と比較し、スチコン3分加熱のほうが加熱時間は長かったにもかかわらず、大量調理機器間で有意な差を認めなかった。バリオおよび鍋加熱後、水さらし時間が3分と長くなるとビタミンCの残存率は有意に低下した(p<0.05)。, In this study, we aimed to investigate the effects of heating methods and watercooling time on the properties of spinach using three types of large-scale cooking equipment, namely, the steam convection oven, the Vario cooking center, and the pan. In the Vario cooking center, the temperature increased quickly at the beginning of heating, and the entire bottom of the pan could be maintained at a high temperature. As a result, the boiling water stayed at a high temperature even after the ingredients were added, and the temperature of the ingredients tended to be high. Soluble solid content was significantly higher after heating for 3 min in the steam convection oven compared with heating for 2 min in the Vario cooker and for 2 min in the pot (p<0.05).Furthermore, the soluble solid content showed a low value following exposure to water after heating. The residual content of vitamin C did not show a significant difference between the three types of cooking equipment, even though the heating time of the steam convection oven (3 min) was longer than that of the Vario cooking center and the pan (2 min). Following heating in the Vario cooking center and the pan, exposure to water for 3 min or longer significantly decreased the residual content of vitamin C (p<0.05).
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- 2023
13. Severe encephalopathy associated with SARS-CoV-2 Omicron BA.1 variant infection in a neonate
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Kenichi Tetsuhara, Satoshi Akamine, Yoshie Matsubara, Shunsuke Fujii, Wataru Kashimada, Kentaro Marutani, Michiko Torio, Yuya Morooka, Nozomu Hanaoka, Tsuguto Fujimoto, Haruna Nakamura-Miwa, Satoru Arai, Keiko Tanaka-Taya, Kenji Furuno, Yumi Mizuno, and Ryutaro Kira
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Developmental Neuroscience ,Pediatrics, Perinatology and Child Health ,Neurology (clinical) ,General Medicine - Published
- 2022
14. Immunological Separation of Bioactive Natural Compounds from Crude Drug Extract and Its Application for Cell-Based Studies
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Takuhiro Uto, Tomoe Ohta, Shunsuke Fujii, and Yukihiro Shoyama
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monoclonal antibody ,immunoaffinity column ,natural compound ,one-step separation method ,crude extract ,knockout extract ,Immunologic diseases. Allergy ,RC581-607 - Abstract
In this study, we present a review on a useful approach, namely, immunoaffinity column coupled with monoclonal antibodies (MAbs), to separate natural compounds and its application for cell-based studies. The immunoaffinity column aids in separating the specific target compound from the crude extract. The column capacity was stable even after more than 10 purification cycles of use under the same conditions. After applying the crude extract to the column, the column was washed with washing buffer and eluted with elution buffer. The elution fraction contained the target compound bound to MAb, whereas the washing fraction was the crude extract, which contained all compounds except a group of target compounds; therefore, the washing fraction was referred to as a knockout (KO) crude extract. Cell-based studies using the KO extract revealed the actual effects of the natural compounds in the crude extract. One-step separation of natural compounds using the immunoaffinity column coupled with MAbs may help in determining the potential functions of natural compounds in crude extracts.
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- 2021
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15. Development of Highly Sensitive Chemiluminescence Enzyme Immunostaining Assay to Determine Glycyrrhizin Content Using Anti-glycyrrhizin Monoclonal Antibody
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Shunsuke, Fujii, Yukihiro, Shoyama, Shuichi, Nomura, and Takuhiro, Uto
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Luminescence ,Plants, Medicinal ,Plant Extracts ,Drug Discovery ,Glycyrrhiza ,Antibodies, Monoclonal ,Medicine, Kampo ,General Chemistry ,General Medicine ,Glycyrrhizic Acid - Abstract
Licorice, the root of Glycyrrhiza spp., is used in a large number of herbal medicines, such as traditional Chinese medicines, Japanese Kampo medicines, and therapeutic drugs. Since glycyrrhizin (GL) is among the main components in licorice and exhibits numerous beneficial pharmacological activities, the content of GL directly affects biological activity. The quality control based on GL content is an important factor in ensuring biological activity; however, the content of GL in licorice varies depending on plant cultivation environment, genetic factors, and species type. Previously, we prepared an anti-GL monoclonal antibody (anti-GL mAb) and employed it in various immunochemical assays for quality control of licorice and licorice-based products. In this study, we employed the anti-GL mAb in chemiluminescence enzyme immunostaining (CLEIS) to develop a very simple, rapid, specific, and sensitive quality control assay for licorice products, with a limit of detection of 3.9 ng. Furthermore, the CLEIS assay enabled semiquantitative analysis of GL in Kampo medicines. Our results showed that multiple samples can be simultaneously analyzed using CLEIS, and it is a useful tool for determining GL content, as well as ensuring chemical quality control of licorice-containing products and herbal medicines.
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- 2022
16. Respiratory failure caused by cardiac dysfunction triggered by bronchiolitis
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Kenichi Tetsuhara, Suzu Imamura, Mamoru Muraoka, and Shunsuke Fujii
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medicine.medical_specialty ,Heart Diseases ,business.industry ,Infant ,General Medicine ,medicine.disease ,Intensive Care Units, Pediatric ,Cardiac dysfunction ,Pulmonary artery banding ,Respiratory failure ,Bronchiolitis ,Internal medicine ,Heart failure ,medicine ,Cardiology ,Humans ,Atrioventricular Septal Defect ,Presentation (obstetrics) ,business ,Trisomy ,Child ,Respiratory Insufficiency ,Retrospective Studies - Abstract
A 3-month-old female weighing 3.6 kg, with trisomy 21, who had undergone pulmonary artery banding for atrioventricular septal defect 2.5 months prior to the presentation was referred to our hospital because of nasal discharge and cough for 3 days and wheezing and decreased feeding for 1 day before
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- 2023
17. The Association between the First Cry and Clinical Outcomes in CDH Neonates: A Retrospective Study
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Yokoi, Yuki Takeuchi, Akiyoshi Nomura, Masaya Yamoto, Satoko Ohfuji, Shunsuke Fujii, Seiji Yoshimoto, Toru Funakoshi, Masato Shinkai, Naoto Urushihara, and Akiko
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congenital diaphragmatic hernia ,first cry ,fetal sedation ,survival - Abstract
Congenital diaphragmatic hernia (CDH) is a life-threatening condition characterized by the herniation of abdominal organs into the thorax, resulting in hypoplastic lungs and pulmonary hypertension. The impact of the first cry, a crucial event for lung transition during birth, on CDH patients remains unclear. This study investigated the impact of the first cry during birth on CDH patient survival, along with other prognosis factors. A multi-institutional retrospective study assessed CDH patient characteristics and survival rates by analyzing factors including the first cry, disease severity, birth weight, Apgar scores, oxygenation index (OI) and surgical closure. Among the CDH patients in the study, a positive first cry was linked to 100% survival, regardless of disease severity (p < 0.001). Notably, the presence of a positive first cry did not significantly affect survival rates in patients with worse prognostic factors, such as low birth weight (
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- 2023
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18. A Survey on Remote Education of‘Introduction to Hospitality’: Comparing 2020 and 2021 Results
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Tomonori, TAKI, Tateo, HASHIMOTO, Atsushi, URAGO, Eriko, AIBA, Viktor, VIRÀG, Naoki, NAKAMURA, Kazuto, ODA, Shunsuke, FUJII, Masashi, FUKASAWA, Tomoaki, ISHIHARA, Keitaro, TANAKA, Kinya, MATSUMOTO, Kokoro, MATSUO, Yukari, HASHIMOTO, and Kyonmi, YOU
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遠隔授業の快適さ ,ホスピタリティ概論 ,受講場所 ,Introduction to Hospitality ,Remote education environment ,Internet access ,Comfortability with taking remote classes ,オンライン学修環境 ,Students’ location of taking remote education class ,アクセス - Abstract
2021年度は「ホスピタリティ概論」を遠隔授業で実施して2年目となった。この科目の受講生を対象に、オンライン学習環境についてのアンケートを行い、2020年の結果と比較した。受講場所は自宅外が増加した。アクセスの円滑さの評価は、やや低下した。画面・音声・提示資料の明瞭性等についての評価は横ばいである。受講中に感じることとして、アクセスへの不安が増加した一方、遠隔授業を受けることの快適さも増加している。自由記述回答での特徴的な頻出語についての調査結果からは、受信環境整備の重要性が示唆されている。, ‘Introduction to Hospitality’ was conducted by remote class in two years in row in 2021. A survey on remote education environment was conducted, and the result was compared with that of 2020 survey. Number of students who took remote class outside home increased. Evaluation of accessibility was slightly decreased. Clarity of the screen, sound and teaching materials remain the same. As for what the students feel during the class, concerns about access increased, while number of students who feel comfortable in taking remote classes increased. Frequent words in free text answer are reported.
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- 2022
19. Enzyme-immobilized Screen-printed Sensor for One Drop of Sample Using Porous Silica Spheres.
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Shunsuke Fujii, Akiko Yoshida, Chuong, Tracy T., Yuka Minegishi, Yuta Nishina, Hirotomo Nishihara, Kouji Masumoto, and Tetsuji Itoh
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POROUS silica ,MESOPOROUS materials ,PLATINUM electrodes ,ELECTRODE reactions ,DETECTORS ,SPHERES - Abstract
A sensor chip that can detect enzyme electrode reactions with a sample volume as small as 20 μL has been developed using screen-printing technology with porous silica spheres (PSSs). PSSs are spherical particles of silica with mesoporous pores, a material with a large specific surface area. Other material properties of PSSs include good dispersibility, high mechanical strength, and the ability to be synthesized with precisely controlled mesopore sizes. These advantages support the increased adsorption of enzymes to create highly sensitive sensors. The electrode was designed as follows: carbon paste (CP) with PSSs was used as the working electrode, glucose dehydrogenase (GDH) was used as the enzyme, and quinoline-5,8-dione (QD) was used as the mediator of electron transfer to induce a glucose dehydrogenation reaction. The sensitivity of the sensor was demonstrated through the detection of glucose. The glucose sensor showed reliable sensitivity between 1 and 60 mM glucose, which is comparable to the detection range of a traditional working electrode such as platinum. Furthermore, the GDH had a minimal reduction in activity over repeated usage. This device demonstrates the ability to create an equally sensitive but less expensive and reusable sensor that has the potential to better suit general applications. [ABSTRACT FROM AUTHOR]
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- 2023
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20. Gastric outlet obstruction caused by an ectopic pancreas in a neonate: A case report
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Shunsuke Fujii, Hideaki Tanaka, Kentaro Ono, Yutaro Onizawa, Yayoi Miyazono, Naoya Sakamoto, Chikashi Gotoh, Yasuhisa Urita, Toko Shinkai, Hajime Takayasu, Ryo Sumazaki, and Kouji Masumoto
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Ectopic pancreas ,Neonate ,Obstruction ,Pediatrics ,RJ1-570 ,Surgery ,RD1-811 - Abstract
We herein report a neonate who presented with non-bilious vomiting at one day of age caused by a prepyloric ectopic pancreas. Ultrasonography clearly detected the presence of a submucosal mass preoperatively, which was treated with local gastric resection. Only 9 neonates with a symptomatic pyloric or prepyloric ectopic pancreas have been previously reported in the literature. Therefore, we reviewed and discussed the clinical features of neonates with this type of ectopic pancreas.
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- 2015
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21. Effects of moist heat on characteristics of vegetables cooked using large-scale cooking equipment
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Ryoko, KIKUCHI, Natsuki, YOSHINAGA, Shunsuke, FUJII, and Takako, KOGA
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heating operation ,湿式加熱 ,加熱操作 ,大量調理機器 ,large-scale cooking equipment ,moist heat ,野菜の調理特性 ,cooking property of vegetable - Abstract
食品に適した大量調理機器を選択し、最適な調理条件を設定することで、嗜好・品質特性を高め、給食の調理における効率化および標準化に繋げる基礎資料を得ることを目的とした。大量調理機器は、鍋、スチコン、バリオを用い、湿式加熱による食品の調理特性を検討した。スチコンが最も加熱時間を要した。スチコン加熱は、湿式加熱のうち蒸気による凝縮熱伝導と熱風蒸し操作であるため、食材の表面から内部へ温度の上昇は緩慢となり、均一な加熱となることで野菜の糖度は高かった。バリオ加熱は、加熱初期の温度上昇が早く、デンプンの糊化が急速に進み、じゃがいもではビタミンCが失われにくかったが、にんじんでは高温で加熱される時間が長くなりビタミンCの分解が進んだ。
- Published
- 2021
22. Liquiritin and Liquiritigenin Induce Melanogenesis via Enhancement of p38 and PKA Signaling Pathways
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Takuhiro Uto, Tomoe Ohta, Akihisa Yamashita, Shunsuke Fujii, and Yukihiro Shoyama
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licorice ,liquiritigenin ,liquiritin ,melanin ,tyrosinase ,TRP-1/2 ,MITF ,p38 ,PKA ,Medicine - Abstract
Background: Liquiritin (LQ) and its aglycone, liquiritigenin (LQG), are major flavonoids in licorice root (Glycyrrhiza spp.). Our preliminary screening identified LQ and LQG, which promote melanin synthesis in the melanoma cells. In this study, we investigated the molecular mechanism of melanin synthesis activated by LQ and LQG. Methods: Murine (B16-F1) and human (HMVII) melanoma cell lines were treated with LQ or LQG. After incubation, melanin contents, intracellular tyrosinase activity, and cell viability were evaluated. Protein levels were determined using Western blotting. Results: LQ and LQG activated melanin synthesis and intracellular tyrosinase activity. The induction of melanin and intracellular tyrosinase activity by LQG was higher than that by LQ. LQ and LQG induced the expression of tyrosinase, tyrosinase-related protein (TRP)-1, and TRP-2. LQ and LQG also enhanced microphthalmia-associated transcription factor (MITF) expression, and cyclic AMP-responsive element-binding protein (CREB) phosphorylation. The phosphorylation of p38 and extracellular signal-regulated kinase (ERK), but not Akt, was significantly increased by LQ or LQG. Furthermore, LQ- or LQG-mediated melanin synthesis was partially blocked by p38 inhibitor (SB203580) and protein kinase A (PKA) inhibitor (H-89); however, ERK kinase (MEK) inhibitor (U0126) and phosphatidylinositol-3-kinase (PI3K) inhibitor (LY294002) had no effect. Conclusions: The results suggest that LQ and LQG enhance melanin synthesis by upregulating the expression of melanogenic enzymes, which were activated by p38 and PKA signaling pathways, leading to MITF expression and CREB phosphorylation.
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- 2019
- Full Text
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23. Development of an indirect competitive enzyme-linked immunosorbent assay for formononetin and its application in a cell-based assay using MC3T3-E1 cells
- Author
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Shunsuke Fujii, Tomoe Ohta, Riho Ehama, Mizuki Irikida, Shuichi Nomura, Yukihiro Shoyama, and Takuhiro Uto
- Subjects
Mice ,Mice, Inbred BALB C ,Hybridomas ,Flavin Mononucleotide ,Animals ,Enzyme-Linked Immunosorbent Assay ,General Medicine ,Isoflavones ,Food Science ,Analytical Chemistry - Abstract
Formononetin (FMN) is a methoxy isoflavone found abundantly in leguminous plants and associated foods. Several analytical methods have been developed to detect FMN. However, they are costly, complicated, and time-consuming. This study describes an indirect competitive enzyme-linked immunosorbent assay (icELISA) to determine FMN content in food samples using a monoclonal antibody (mAb) against FMN produced by a newly established hybridoma cell line. Validation studies were conducted, and this assay was found to be sufficiently reliable, with an analytical measurement range of 19.53-1250 ng/mL and a detection limit of 17.42 ng/mL. Furthermore, icELISA was successfully applied for a cell-based assay in which the amount of FMN and ononin uptake was quantified in MC3T3-E1 cells. Hence, icELISA is a simple and reliable method for the detection and quantification of FMN, as well as elucidation of its functions and underlying mechanisms of action.
- Published
- 2023
24. Flavin Adenine Dinucleotide-Related Adverse Event Necessitating Cardiopulmonary Resuscitation in an Infant
- Author
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Shunsuke Fujii, Kenichi Tetsuhara, Suzu Imamura, Mamoru Muraoka, and Sayo Suzuki
- Subjects
General Engineering - Published
- 2022
25. Applications of Monoclonal Antibodies Against Natural Compounds for Functional Analysis of Crude Drugs
- Author
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Takuhiro Uto, Tomoe Ohta, Yukihiro Shoyama, Shunsuke Fujii, and Seiichi Sakamoto
- Subjects
0301 basic medicine ,Pharmacology ,medicine.drug_class ,Cell ,Harringtonine ,Monoclonal antibody ,Eastern blot ,Biochemistry ,03 medical and health sciences ,chemistry.chemical_compound ,030104 developmental biology ,0302 clinical medicine ,medicine.anatomical_structure ,chemistry ,Pharmacokinetics ,030220 oncology & carcinogenesis ,Drug Discovery ,Genetics ,medicine ,Glycyrrhizin ,Carcinogen ,Cellular localization - Abstract
This study summarizes the applications of monoclonal antibodies (MAbs) against bioactive natural compounds for the functional analysis of crude drugs. To date, we have established more than 40 kinds of MAbs against natural compounds and set up enzyme-linked immunosorbent assay (ELISA). The intracellular accumulation of harringtonine (HT) and its derivatives into cells was determined by ELISA using anti-HT MAb, and the uptake levels of these compounds conformed to their respective antiproliferative activities. Eastern blotting is an on-membrane quantitative analytical system that uses MAbs against natural compounds and it can also be used in a biological sample for pharmacokinetics study. Glycyrrhizin (GC) was detected in the serum of rats treated with GC by eastern blotting. Additionally, eastern blotting using MAb against 3-monoglucuronyl-glycyrrhetinic acid (3-MGA) could determine a potential causative agent of pseudoaldosteronism induced by excess intake of GC. The immunoaffinity column coupled with MAb made preparing an extract containing all components feasible except the target compound. Cell-based studies using GC-removed licorice extract, which was prepared by the immunoaffinity column conjugated with anti-GC MAb, revealed the potential functions of GC in licorice extract. Moreover, MAbs were used in determining cellular localization and target molecules of natural compounds. The combination of analysis using MAb and LC-MS/MS showed that aristolochic acid I (AA-I), a naturally occurring nephrotoxin and carcinogen, targets α-actinin-4 in the kidney cells. MAbs against natural compounds are useful tools to determine real pharmacological functions, cellular localization, and molecular targets of natural compounds in crude extracts.
- Published
- 2020
26. Kwakhurin‐magnetic particles conjugates enable fast enzyme immunoassay for the detection of kwakhurin in Pueraria candollei
- Author
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Poomraphie Nuntawong, Seiichi Sakamoto, Shunsuke Fujii, Hiroyuki Tanaka, Gorawit Yusakul, Waraporn Putalun, Ryota Eto, Thaweesak Juengwatanatrakul, and Satoshi Morimoto
- Subjects
Enzyme-Linked Immunosorbent Assay ,Plant Science ,01 natural sciences ,Biochemistry ,Analytical Chemistry ,Immunoenzyme Techniques ,Drug Discovery ,medicine ,Humans ,Detection limit ,chemistry.chemical_classification ,Folk medicine ,Chromatography ,medicine.diagnostic_test ,Kwakhurin ,Magnetic Phenomena ,010401 analytical chemistry ,General Medicine ,magnetic particles-based enzyme immunoassay ,Thailand ,Isoflavones ,0104 chemical sciences ,food safety ,Pueraria ,010404 medicinal & biomolecular chemistry ,Enzyme ,Complementary and alternative medicine ,chemistry ,monoclonal antibody ,Immunoassay ,Pueraria candollei ,Molecular Medicine ,Female ,Steroids ,Quantitative analysis (chemistry) ,Food Science ,Conjugate - Abstract
Introduction Kwakhurin (Kwa) is one of the unique isoflavonoids produced in Pueraria candollei var. mirifica (P. candollei), which has long been used as folk medicine for rejuvenation in Thailand. Recently, the use of P. candollei-derived products has widely spread among Japanese women for cosmetic purposes. Correspondingly, there has been an increase in the number of reports regarding possible health hazards caused by estrogenic activity inherent to the plant; thus, the need for a detailed evaluation of the phytoestrogen content of P. candollei-derived products has gained a sense of urgency in recent years. Objective This study aims to develop a rapid enzyme immunoassay that can be applied to the quantitative analysis of Kwa in P. candollei and its derived products. Material and method A rapid and sensitive immunoassay was developed with a combination of Kwa-specific monoclonal antibody (MAb 11F) and Kwa-magnetic particles (MPs) conjugates, which increased the surface area of the solid phase, resulting in a decrease in the immunoreaction time. Result This novel MPs-based enzyme immunoassay (MPs-EIA) was used to determine Kwa concentration in the range from 2.44 to 78.1 ng/mL with a limit of detection of 1.90 ng/mL. Validation analyses revealed that the proposed MPs-EIA protocol was sufficiently precise and accurate for effective quantitative analysis of Kwa in P. candollei and its derived products.
- Published
- 2020
27. Current status and issues in a first year experience program to ensure understanding in school philosophy: -From the results of class room surveys in Introduction to Hospitality
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Toshiyuki, FUJIWARA, Hideya, INOUE, Toshiaki, YANO, Masahiro, MATSUNAGA, Kinya, MATSUMOT, Miho, YAMAUCHI, Shutaro, JINNO, Masayuki, MORIO, Yuka, KANAZAWA, Viktor, VIRÀG, Kazuto, ODA, Shunsuke, FUJII, Keitaro, TANAKA, Tsukasa, FUJIKI, Ryuji, INOUE, Takashi, SAKAMOTO, Koji, TAMURA, Yukari, HASHIMOTO, Takamasa, KOBAYASHI, and Tateo, HASHIMOTO
- Subjects
教職協働 ,ホスピタリティ概論 ,初年次教育 ,建学の精神 ,School philosophy, first year experience program, Introduction to hospitality, Collaborative work between faculty and academic staff - Abstract
本研究は、2017年度から始まった本学の初年次教育を定着させるとともに、新入生が大学での教育を理解し、建学の精神に代表される理念やディプロマポリシーを自分のものとして捉え、その実現に向けた主体的な学修の基盤を構築するという初年次教育の目標の達成を図ることにある。このためには、彼らの特性をとらえるとともに、彼らが初年次教育をどのように捉え、理解したかを把握しなければならない。そこで初年次教育の科目の一つとして開設された「ホスピタリティ概論」を活用して、全受講生を対象とした2つの調査を3年間実施した。1つ目の調査は入学当初の学生像をとらえ授業展開に生かすために学生の特徴を把握するものであり、第1回目の授業で実施された。2つ目の調査は授業内容の理解度を評価するものであり、第10回目に実施された。これらの回答を年度と学科毎に集計、分析した。その結果、次のことが明らかになった。①オープンキャンパスへの参加や進学希望状況などの本学入学に至る経緯が学科によって異なること、そして、入学当初には心身に不安を抱いている学生が、どの学科にも相当数存在すること。3年間の調査によって各学科の学生の特色が明らかになり、学科毎の適切な学修支援方策の作成に向けた示唆が得られたこと。②この講義を受けて、建学の精神であるホスピタリティの獲得に大学が力を入れており、その実現が求められていると多くの学生が認識していること、及び、本科目の重点目標である多様性の理解力の育成につながる他学科の学生とのコミュニケーションの機会に意義を見出していること等から、授業の目標達成に向けた順調な歩みが見られること。これらの結果及び他の科目の報告から、初年次教育が定着しつつあること、そして、その目標達成に向けた歩みが順調であると判断した。ただ、更なる充実のための課題も浮かび上がっている。
- Published
- 2020
28. Retrospective Study of Image Quality in Pediatric Cardiac Cine MRI
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Shunsuke Fujii, Akira Kurozumi, Toshi Matsushita, Mitsugi Honda, and Naoki Nishida
- Subjects
Adult ,Male ,Image quality ,Magnetic Resonance Imaging, Cine ,030204 cardiovascular system & hematology ,030218 nuclear medicine & medical imaging ,03 medical and health sciences ,0302 clinical medicine ,Heart Rate ,Heart rate ,Humans ,Medicine ,Segmentation ,Child ,Retrospective Studies ,Ejection fraction ,medicine.diagnostic_test ,business.industry ,Significant difference ,Infant ,Reproducibility of Results ,Heart ,Retrospective cohort study ,Magnetic resonance imaging ,General Medicine ,Image Enhancement ,Cine mri ,Child, Preschool ,Female ,Nuclear medicine ,business ,Algorithms - Abstract
In pediatric cardiac cine magnetic resonance imaging (MRI), it must overcome several challenges including the patient's size and higher heart rate. The aim of this study was to retrospectively evaluate imaging optimization. Cardiac cine MRI data from 24 patients was analyzed (age range: 3 months-10 years, average age: 5 years, male/female: 11/13, R-R interval: 450±4 to 819±7 ms). About 11 cases out of 24 have good image quality. For small variations in the R-R interval and higher temporal resolution improved image quality with significant difference (P
- Published
- 2020
29. Structural Characterization of the Body Frame and Spicules of a Glass Sponge
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Akane Arasuna, Masahito Kigawa, Shunsuke Fujii, Takatsugu Endo, Kenji Takahashi, and Masayuki Okuno
- Subjects
sponge ,biogenic silica ,nanostructure ,silanol ,ring structure ,amorphous silica ,Raman spectroscopy ,Mineralogy ,QE351-399.2 - Abstract
The nanostructure (atomic-scale structure) and water species in the body frame and spicules of the marine glass sponge, Euplectella aspergillum, collected from the sea floor around Cebu Island was characterized in detail by thermogravimetric differential thermal analysis, nuclear magnetic resonance spectroscopy, Raman and infrared spectroscopies, and X-ray diffraction method. The structural features of the nanostructure in the body frame and spicules were essentially similar to each other, although these were different from those of inorganic amorphous silica materials, such as silica gel and silica glass. In addition, the averaged short and medium range structures of the sponge may be similar to those of tridymite. The water content and water species included in the body frame and spicules were almost the same. More than half of the contained water was physisorbed water molecules, and the rest was attributed to Q3 and Q2 silanol groups. Most of the water species may be present at the surface and involved in hydrogen bonding.
- Published
- 2018
- Full Text
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30. Development of Small-sized Lysine Enzyme Sensor for Clinical Use
- Author
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Takeshi Uemura, Shunsuke Fujii, Hiroki Yamazaki, Tetsuji Itoh, Kouji Masumoto, and Seiichi Nishizawa
- Subjects
General Materials Science ,Instrumentation - Published
- 2022
31. Analyzing Class room Survey to Broaden First Year Experience in Nagasaki International University -In the Case of Introduction to Hospitality
- Author
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Hideya, INOUE, Toshiyuki, FUJIWARA, Masahiro, MATSUNAGA, Kinya, MATSUMOTO, Tetsuhiro, HARA, Tsubasa, OTOSU, Masayuki, MORIO, Ken, NODA, Virag, VIKTOR, Kazuto, ODA, Shunsuke, FUJII, Keitaro, TANAKA, Yuuki, FUJII, Ryuji, INOUE, Takasi, SAKAMOTO, Hiroaki, MATSUGUCHI, Tetsuo, FUKUNARI, Yukari, HASHIMOTO, Takamasa, KOBAYASHI, and Tateo, HASHIMOTO
- Subjects
教職協働 ,higher education, first year experience, Collaborative work between faculty and academic staff, peer support ,高等教育 ,初年次教育 ,ピアサポート - Abstract
本研究は、初年次教育の深化を図るために、その一つの科目である「ホスピタリティ概論」を対象として、受講生の感想等を分析し、授業の改善を図ることを目的としている。2017年度に開設された「ホスピタリティ概論」は、2年目を迎えるにあたって、前回の調査で明らかになった課題の幾つかに改善を加えて実践された1)。加えられた改善点は、上級生を学生スタッフとして雇用し、受講生の学修活動の促進を図ること、そして各課割り当てからボランティアの事務職員を担当にし、教職協働体制の円滑化を図ることの2点である。昨年度同様のクリッカー調査とアンケート調査を実施し、2年間の結果の比較・分析を行った。この結果、次のことが明らかになった。クリッカー調査 ①平成30年度の方が29年度よりも、心身の不安を訴える1年生が多いこと。 ②入学の動機づけとなるオープンキャンパスへの参加に関しては、平成30年度の方が、複数回参加や家族の参加が多くなっており、参加しなかった割合が低くなっていること。③全学オリエンテーションと学科別オリエンテーションの理解度に関しては、共に平成29年度の方が高い数値を示していること。アンケート調査 ④「他学科の人とおしゃべりする機会が持てた」や、「他学科の人の意見を聞くことができて有意義だった」という授業内でのコミュニケーション関しては、平成30年度の方が高い評価が得られていること。⑤「ホスピタリティを理解する上で、この授業に合格点を与えることができる」に関しては、平成30年度の方が高い評価が得られていること。 ⑥自由記述欄の文字数の調査では、平成30年度の方が高い数値になっていること。上述の①~③に示されているように、年度によって学生の質的な変化が生じているように見え、今後の動向を注視しなければならないことが明らかになった。また、④~⑥の結果からは、今年度の改善点である学生スタッフの活用によって、「ホスピタリティ概論」における学生の学修活動の活性化をもたらしたと判断する事ができる。しかし、他の項目では学科による差も見られ、課題が生じているといえる。
- Published
- 2019
32. Improvement of Brain Contrast Using Spin Echo T1-weighted Image at 3 T MRI
- Author
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Toshi Matsushita, Akira Kurozumi, Yoshito Ichiba, Seiichiro Ohno, Seiji Tahara, Shunsuke Fujii, and Akira Nagano
- Subjects
Physics ,medicine.diagnostic_test ,Fat suppression ,Magnetic resonance imaging ,T1 contrast ,General Medicine ,Nuclear magnetic resonance ,Amplitude ,Flip angle ,Pulse-amplitude modulation ,medicine ,Spin echo ,Radio frequency ,human activities - Abstract
Brain T1-weighted images using spin echo (SE) sequence has poor contrast at 3.0 Tesla magnetic resonance imaging (3.0 T MRI) systems from the influence of crosstalk and magnetized transfer (MT) effect, and prolongation of the T1 value. Therefore, improving of scan parameters has been reported such as excitation flip angle (FA) and interleave data acquisition. The purpose of this study was to show the effects of alterations of presaturation pulse amplitude and chemical shift selective (CHESS) pulse amplitude. Gray-to-white matter contrast increased with decreasing amplitude of presaturation pulse in whole brain imaging. Presaturation and CHESS pulse consist of radio frequency pulse. Therefore, both pulses have a similar effect on MT pulse. Manual alteration of presaturation pulse amplitude for each scan lacks versatility on clinical use. However, decreasing amplitude of presaturation pulse is equal to decreasing thickness of presaturation pulse. About CHESS pulse, it requires no manual alteration for each scan. For example, switching fat suppression mode from strong to weak increase T1 contrast. Our study demonstrated that using not only low excitation FA and interleave date acquisition but also low amplitude of presaturation and CHESS pulse increase the contrast in T1 SE brain scans at 3.0 T MRI.
- Published
- 2019
33. Nano-Structural Investigation on Cellulose Highly Dissolved in Ionic Liquid: A Small Angle X-ray Scattering Study
- Author
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Takatsugu Endo, Shota Hosomi, Shunsuke Fujii, Kazuaki Ninomiya, and Kenji Takahashi
- Subjects
ionic liquids ,cellulose ,small angle X-ray scattering ,dissolution ,composites ,Organic chemistry ,QD241-441 - Abstract
We investigated nano-structural changes of cellulose dissolved in 1-ethyl-3-methylimidazolium acetate—an ionic liquid (IL)—using a small angle X-ray scattering (SAXS) technique over the entire concentration range (0–100 mol %). Fibril structures of cellulose disappeared at 40 mol % of cellulose, which is a significantly higher concentration than the maximum concentration of dissolution (24–28 mol %) previously determined in this IL. This behavior is explained by the presence of the anion bridging, whereby an anion prefers to interact with multiple OH groups of different cellulose molecules at high concentrations, discovered in our recent work. Furthermore, we observed the emergence of two aggregated nano-structures in the concentration range of 30–80 mol %. The diameter of one structure was 12–20 nm, dependent on concentration, which is ascribed to cellulose chain entanglement. In contrast, the other with 4.1 nm diameter exhibited concentration independence and is reminiscent of a cellulose microfibril, reflecting the occurrence of nanofibrillation. These results contribute to an understanding of the dissolution mechanism of cellulose in ILs. Finally, we unexpectedly proposed a novel cellulose/IL composite: the cellulose/IL mixtures of 30–50 mol % that possess liquid crystallinity are sufficiently hard to be moldable.
- Published
- 2017
- Full Text
- View/download PDF
34. Saffron (Crocus sativus L.) as a valuable spice and food product: a narrative review
- Author
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Shunsuke Fujii, Yumi Morita, Tomoe Ohta, Takuhiro Uto, and Yukihiro Shoyama
- Published
- 2022
35. Rapid magnetic particles-based enzyme immunoassay for the quality control of Glycyrrhiza spp. based on glycyrrhizin content
- Author
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Waraporn Putalun, Seiichi Sakamoto, Shunsuke Fujii, Hiroyuki Tanaka, Satoshi Morimoto, Kei Minami, and Gorawit Yusakul
- Subjects
Quality Control ,medicine.drug_class ,Monoclonal antibody ,Immunoenzyme Techniques ,chemistry.chemical_compound ,Japan ,Drug Discovery ,medicine ,Glycyrrhiza ,Glycyrrhizin ,Triterpenoid saponin ,Pharmacology ,chemistry.chemical_classification ,Detection limit ,Chromatography ,Plants, Medicinal ,medicine.diagnostic_test ,biology ,Magnetic Phenomena ,Antibodies, Monoclonal ,General Medicine ,biology.organism_classification ,Glycyrrhizic Acid ,Primary and secondary antibodies ,Japanese Pharmacopoeia ,chemistry ,Immunoassay ,biology.protein ,Plant Preparations - Abstract
Glycyrrhizin (GC) is a triterpenoid saponin isolated from the roots of Glycyrrhiza spp., a medicinal plant that is present in 70% of Kampo prescriptions. Since the GC content in Glycyrrhiza spp. affects its various pharmacological activities, Glycyrrhiza spp. is prescribed to contain at least 2% of GC in the Japanese pharmacopoeia, and its quality control based on GC content is required. In this study, a magnetic particles-based enzyme immunoassay (MPs-EIA) was developed using specific monoclonal antibody against GC (MAb 2H2) for the detection of GC in Glycyrrhiza spp. In this system, the immunoreaction time using primary and secondary antibodies was reduced by taking advantage of the wide surface area of magnetic particles (MPs) conjugated with GC by N,N′‑carbonyldiimidazole (CDI)-mediated method. Optimization of MPs-EIA revealed that total assay time (~2 h) was reduced to over half of that of conventional indirect competitive enzyme-linked immunosorbent assay (ELISA) (~5 h). In addition, the GC concentration was detectable within the range from 97.7 to 781 ng/mL, with a limit of detection of 71.4 ng/mL. A series of further validation analyses support the reliability and accuracy of the developed MPs-EIA for the detection of GC in Glycyrrhiza spp. Since the present MPs-EIA overcomes the disadvantage of ELISA in terms of rapidity, it provides a useful approach for the effective quality control of Glycyrrhiza spp., especially when handling multiple samples.
- Published
- 2020
36. Esophageal stent for a recurrent tracheoesophageal fistula in an infant
- Author
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Shunsuke Fujii, Kaori Okamura, Keiichiro Mizuno, Makoto Hayashida, and Sooyoung Lee
- Subjects
medicine.medical_specialty ,business.industry ,MEDLINE ,Infant ,Tracheoesophageal fistula ,medicine.disease ,Hypoplastic left heart syndrome ,Surgery ,Palliative Therapy ,Esophageal stent ,Pediatrics, Perinatology and Child Health ,Medicine ,Humans ,Stents ,business ,Esophageal Atresia ,Tracheoesophageal Fistula - Published
- 2020
37. Efficient pretreatment of bagasse at high loading in an ionic liquid
- Author
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Kazuaki Ninomiya, Ei Mon Aung, Shunsuke Fujii, Kenji Takahashi, Kosuke Kuroda, and Takatsugu Endo
- Subjects
010405 organic chemistry ,Biomass ,Lignocellulosic biomass ,02 engineering and technology ,021001 nanoscience & nanotechnology ,01 natural sciences ,0104 chemical sciences ,Microcrystalline cellulose ,chemistry.chemical_compound ,Hydrolysis ,chemistry ,Chemical engineering ,Acid hydrolysis ,Biorefining ,Cellulose ,0210 nano-technology ,Bagasse ,Agronomy and Crop Science - Abstract
A high biomass loading in ionic liquid (IL) pretreatments that reduces the total cost of the process is potentially a key technique in biorefining. Previously, we demonstrated that, with microcrystalline cellulose, a higher cellulose loading could lead to improved accessibility and consequently higher hydrolytic reactivity, when catalyzed by an acid. In this study, we expand this concept to the lignocellulosic biomass such as bagasse. The pretreatment of bagasse was performed with a representative IL,1-ethyl-3-methylimidazolium acetate, over a biomass loading range of 5–50 wt% and was subsequently subjected to crystallinity measurements and acid hydrolysis. The results indicated a criterion of the pretreatment condition, which showed a higher pretreatment efficiency at a higher biomass loading. At the optimum pretreatment condition, the hydrolytic reactivity for 33 wt% biomass loading was 1.4 times higher than that at the conventional loading of 5 wt%. This concurred with the results that demonstrated a high biomass loading condition produces a lower crystallinity.
- Published
- 2018
38. Cellulose structural change in various biomass species pretreated by ionic liquid at different biomass loadings
- Author
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Takatsugu Endo, Shunsuke Fujii, Ei Mon Aung, Kosuke Kuroda, Takayuki Tsukegi, Kazuaki Ninomiya, and Kenji Takahashi
- Subjects
Environmental Engineering ,Bioengineering ,Waste Management and Disposal - Abstract
High biomass loading is a key technique to reduce the pretreatment cost of lignocellulosic biomass. In this work, various biomass species such as bagasse, erianthus, cedar, and eucalyptus were pretreated using an ionic liquid, 1-ethyl-3-methylimidazolium acetate, at different biomass loadings, particularly focusing on a high loading region. Cellulose structural changes in pretreated biomass were investigated via X-ray scattering and 13C solid-state nuclear magnetic resonance (SSNMR) spectroscopy. The structural behaviors roughly fell into two categories, corresponding to either grassy (bagasse and erianthus) or woody (cedar and hardwood) biomass. The grassy biomass gradually transformed from cellulose-I to cellulose-II in a monotonic manner against the biomass loading. In contrast, the transformation in the woody biomass occurred abruptly as solids was decreased within the high loadings range (50 wt% to 33 wt%). Below 33 wt%, a reformation of cellulose-I from cellulose-II proceeded readily. In terms of cellulose crystallinity, erianthus as well as bagasse showed a minimum value at 25 wt% loading, whereas the crystallinity for the woody biomass did not possess such a clear minimum. Acid hydrolysis of these pretreated biomass was also conducted and the relationship between the reactivity and the cellulose structural changes was discussed.
- Published
- 2018
39. Preparation of Anti-Glycyrrhetinic Acid Monoclonal Antibody for Application in an Indirect Competitive Enzyme-Linked Immunosorbent Assay
- Author
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Yukihiro Shoyama, Shunsuke Fujii, Shuichi Nomura, and Takuhiro Uto
- Subjects
Acid concentration ,medicine.drug_class ,medicine.medical_treatment ,Metabolite ,Clinical Biochemistry ,Monoclonal antibody ,01 natural sciences ,Biochemistry ,Analytical Chemistry ,03 medical and health sciences ,chemistry.chemical_compound ,0302 clinical medicine ,Electrochemistry ,Splenocyte ,medicine ,Glycyrrhizin ,Spectroscopy ,chemistry.chemical_classification ,Chromatography ,010401 analytical chemistry ,Biochemistry (medical) ,Hemocyanin ,0104 chemical sciences ,Hybridoma cell ,Enzyme ,chemistry ,030220 oncology & carcinogenesis - Abstract
Glycyrrhetinic acid is a major metabolite of glycyrrhizin, which is one of the main components of licorice roots and is considered to be one of the pharmacologically active substances in licorice. A new hybridoma cell line, named G-2A6, was generated by fusing mouse myeloma cells and splenocytes, which were immunized using glycyrrhetinic acid-keyhole limpet hemocyanin to produce a monoclonal antibody (mAb) against glycyrrhetinic acid. By using the anti-glycyrrhetinic acid mAb, we attempted to develop a simple, rapid, and highly sensitive indirect competitive enzyme-linked immunosorbent assay (icELISA). The developed icELISA had a range from 3.91 to 125 ng/ mL with low coefficients of variation (less than 5%) and demonstrated a high recovery rate of glycyrrhetinic acid spiked into licorice powder (average = 101.76%). In addition, the icELISA could determine the glycyrrhetinic acid concentration in glycyrrhetinic acid-spiked human serum with simple pretreatment, which suggests that the developed ELI...
- Published
- 2018
40. Development of amorphous bulk Al-Mn-Si for nano-structured thermoelectric materials
- Author
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Masahiro Adachi, Yoshiyuki Yamamoto, Akio Yamamoto, Shunsuke Nishino, Tsunehiro Takeuchi, Makoto Kiyama, Muthusamy Omprakash, Akihiro Makino, and Shunsuke Fujii
- Subjects
010302 applied physics ,Materials science ,Alloy ,02 engineering and technology ,engineering.material ,021001 nanoscience & nanotechnology ,Thermoelectric materials ,01 natural sciences ,Amorphous phase ,Amorphous solid ,Chemical engineering ,0103 physical sciences ,Nano ,Ribbon ,engineering ,0210 nano-technology - Abstract
In this study, we have developed amorphous bulk in the Al-Mn-Si alloy system as a precursor of thermoelectric materials consisting of nano-grains. The quenched ribbon samples at the composition of Al34.4Mn21.2Si44.4 became single amorphous phase. By applying approximately 3 GPa at room temperature, we succeeded in preparing bulk amorphous samples without possessing any other crystalline phases.
- Published
- 2018
41. Informational theory of relativity
- Author
-
Shunsuke Fujii and Akio Hosoya
- Subjects
Physics ,Geodesic ,010308 nuclear & particles physics ,FOS: Physical sciences ,General Physics and Astronomy ,General Relativity and Quantum Cosmology (gr-qc) ,01 natural sciences ,General Relativity and Quantum Cosmology ,Clock synchronization ,Action (physics) ,symbols.namesake ,Theory of relativity ,Classical mechanics ,Lagrange multiplier ,0103 physical sciences ,Metric (mathematics) ,symbols ,Vector field ,010306 general physics ,Effective action - Abstract
Assuming the minimal time to send a bit of information in the Einstein clock synchronization of the two clocks located at different positions, we introduce the extended metric to the information space. This modification of relativity changes the red shift formula keeping the geodesic equation intact. Extending the gauge symmetry hidden in the metric to the 5-dimensional general invariance, we start with the Einstein-Hilbert action in the 5-dimensional space-time. After the 4+1 decomposition, we obtain the effective action which includes the Einstein-Hilbert action for gravity, the Maxwell-like action for the velocity field and the Lagrange multiplier term which ensures the normalization of the time-like velocity field. As an application, we investigate a solution of the field equations in the case that a 4-dimensional part of the extended metric is spherically symmetric, which exhibits Schwarzschild-like space-time but with the minimal radius. As a discussion we present a possible informational model of synchronization process which is inherently stochastic. The model enables us to interpret the information quantity as a new spatial coordinate., 34 pages, 7 figures
- Published
- 2018
42. A study on the representation of planimetric features in old castle town maps of TAKAYAMA
- Author
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Shunsuke Fujii
- Published
- 2016
43. Pulmonary interstitial emphysema due to respiratory syncytial virus infection
- Author
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Makoto Nakao, Toko Shinkai, Kouji Masumoto, Fumiko Chiba, Hironori Imai, Chikashi Gotoh, Shunsuke Fujii, Hideaki Tanaka, Hajime Takayasu, Yasunari Tanaka, Tsubasa Aiyoshi, Takato Sasaki, Naoya Sakamoto, and Yasuhisa Urita
- Subjects
Mechanical ventilation ,Pathology ,medicine.medical_specialty ,Lung ,Respiratory distress ,business.industry ,medicine.medical_treatment ,Clinical course ,Respiratory infection ,Pulmonary interstitial emphysema ,medicine.disease ,Virus ,03 medical and health sciences ,0302 clinical medicine ,medicine.anatomical_structure ,030225 pediatrics ,embryonic structures ,Pediatrics, Perinatology and Child Health ,medicine ,030212 general & internal medicine ,Respiratory system ,business - Abstract
Pulmonary interstitial emphysema (PIE) primarily affects premature infants on positive pressure ventilation. PIE is rarely reported in infants and children in the absence of mechanical ventilation and/or associated respiratory infection. We report a case of PIE in a 22-month-old girl who had severe respiratory distress due to respiratory syncytial virus infection. Chest computed tomography showed cystic lung lesions mimicking congenital cystic adenomatoid malformation. The cystic lesions spontaneously resolved after conservative treatment. Based on the clinical course and the chronological changes on imaging, the cystic lung lesions were diagnosed as localized persistent PIE.
- Published
- 2016
44. Lung metastasectomy for pediatric solid tumors: Which tumor should we operate?
- Author
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Norihiko Kitagawa, Hidehito Usui, Kyoko Mochizuki, Masato Shinkai, Shunsuke Fujii, Issei Kawakita, Yukihiro Tsuduki, Shota Shinohara, Hiroaki Goto, Kumiko Nozawa, and Yukichi Tanaka
- Subjects
Oncology ,Pediatrics, Perinatology and Child Health ,Hematology - Published
- 2020
45. Perioperative Amino Acid Infusion for Preventing Hypothermia and Improving Clinical Outcomes During Surgery Under General Anesthesia: A Systematic Review and Meta-analysis
- Author
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Kyoko Yanagida, Yoshitaka Aoki, Rintaro Nakau, Kazuyuki Atsumi, Shunsuke Fujii, Yukie Aoshima, Ryo Kaminaka, Junichiro Yokoyama, and Makiko Kora
- Subjects
medicine.medical_specialty ,Hypothermia ,Anesthesia, General ,Placebo ,Perioperative Care ,law.invention ,03 medical and health sciences ,0302 clinical medicine ,Randomized controlled trial ,Infusion therapy ,030202 anesthesiology ,law ,medicine ,Humans ,030212 general & internal medicine ,Amino Acids ,Intraoperative Complications ,Randomized Controlled Trials as Topic ,business.industry ,Publication bias ,Perioperative ,Surgery ,Anesthesiology and Pain Medicine ,Treatment Outcome ,Relative risk ,Meta-analysis ,Anesthesia ,Shivering ,medicine.symptom ,business - Abstract
Amino acid (AA) infusion is sometimes selected to avoid hypothermia during general anesthesia. However, the widespread clinical use of AA infusion therapy has not been established. This study aimed to clarify the evidence that AA infusion can increase patient body temperature and improve clinical outcomes using the Grading of Recommendations Assessment, Development, and Evaluation system. We searched MEDLINE (PubMed), Cochrane Central Register of Controlled Trials, and Igaku Chuo Zasshi (Japana Centra Revuo Medicina) in November 2015. Studies were reviewed by 2 independent assessors to identify randomized controlled trials (RCTs) involving AA infusion compared with placebos during surgery under general or combined general/epidural anesthesia. Study quality was assessed using the Grading of Recommendations Assessment, Development, and Evaluation system and the Cochrane methodology. The primary outcome was difference in body temperature before and after perioperative AA infusion. Shivering frequency, blood loss volume, postoperative intubation time, and hospitalization period were also assessed as clinical outcomes. We analyzed the outcome data using a random effect model. From 298 screened titles, 14 RCTs met our inclusion criteria, including 626 patients (327 in AA and 299 in placebo groups). In 626 participants from 14 RCTs, AA infusion increased body temperature by a mean difference (MD) of 0.46°C (95% confidence interval [CI], 0.31-0.62, low-quality evidence). Regarding other outcomes, AA infusion decreased shivering frequency by a risk ratio of 0.34 (95% CI, 0.12-0.94; 7 RCTs, 248 participants, very low-quality evidence), shortened postoperative intubation time by MD of -125 minutes (95% CI, -210 to -38.8; 2 RCTs, 158 participants, moderate-quality evidence), and shortened the hospitalization period by MD of -1.81 days (95% CI, -2.07 to -1.55; 3 RCTs, 230 participants, low-quality evidence) compared with placebo. There was no significant difference in the volume of blood loss between the 2 groups (standardized MD, -0.20, 95% CI, -0.44 to 0.04; low-quality evidence). There was no publication bias. AA infusion in the perioperative period increased patient body temperature and improved clinical outcomes compared with placebo. However, the evidence to support the use of AA infusion is limited, and further large-scale RCTs are required.
- Published
- 2017
46. Investigation of accessibility and reactivity of cellulose pretreated by ionic liquid at high loading
- Author
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Kenji Takahashi, Kazuaki Ninomiya, Shota Hosomi, Takatsugu Endo, Mitsugu Kimizu, Ei Mon Aung, and Shunsuke Fujii
- Subjects
0106 biological sciences ,Chromatography ,Polymers and Plastics ,010405 organic chemistry ,Chemistry ,Organic Chemistry ,01 natural sciences ,0104 chemical sciences ,Microcrystalline cellulose ,chemistry.chemical_compound ,Crystallinity ,Hydrolysis ,Chemical engineering ,010608 biotechnology ,Specific surface area ,Enzymatic hydrolysis ,Ionic liquid ,Materials Chemistry ,Biorefining ,Cellulose - Abstract
High loading of cellulose in ionic liquid (IL) pretreatment is potentially a key technique for cellulose conversion to glucose in biorefining. In this work, to expand the potential use of this high loading technique, the accessibility of microcrystalline cellulose pretreated with an IL across a wide cellulose loading range (5–50 mol%) and its relationship with the hydrolytic reactivity were comprehensively investigated. The results show that the estimated cellulose accessibility based on the crystallinity and specific surface area was notably higher in 25 mol% loading than that for a conventional loading of 5 mol%. Consistently, acid-catalyzed glucose conversion was faster at this high loading, showing that a higher cellulose loading improves the pretreatment efficiency. In contrast, enzymatic hydrolysis was not enhanced by a high cellulose loading. A key difference between the activities in these two hydrolytic reactions is the catalyst size.
- Published
- 2017
47. Nano-Structural Investigation on Cellulose Highly Dissolved in Ionic Liquid: A Small Angle X-ray Scattering Study
- Author
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Kazuaki Ninomiya, Kenji Takahashi, Shunsuke Fujii, Takatsugu Endo, and Shota Hosomi
- Subjects
small angle X-ray scattering ,ionic liquids ,cellulose ,dissolution ,composites ,Pharmaceutical Science ,02 engineering and technology ,010402 general chemistry ,Fibril ,01 natural sciences ,Article ,Analytical Chemistry ,lcsh:QD241-441 ,chemistry.chemical_compound ,Crystallinity ,lcsh:Organic chemistry ,Drug Discovery ,Polymer chemistry ,Scattering, Small Angle ,Molecule ,Physical and Theoretical Chemistry ,Cellulose ,Particle Size ,Dissolution ,Small-angle X-ray scattering ,Organic Chemistry ,Imidazoles ,021001 nanoscience & nanotechnology ,0104 chemical sciences ,Cellulose microfibril ,Nanostructures ,chemistry ,Chemical engineering ,Chemistry (miscellaneous) ,Ionic liquid ,Molecular Medicine ,0210 nano-technology - Abstract
We investigated nano-structural changes of cellulose dissolved in 1-ethyl-3-methylimidazolium acetate—an ionic liquid (IL)—using a small angle X-ray scattering (SAXS) technique over the entire concentration range (0–100 mol %). Fibril structures of cellulose disappeared at 40 mol % of cellulose, which is a significantly higher concentration than the maximum concentration of dissolution (24–28 mol %) previously determined in this IL. This behavior is explained by the presence of the anion bridging, whereby an anion prefers to interact with multiple OH groups of different cellulose molecules at high concentrations, discovered in our recent work. Furthermore, we observed the emergence of two aggregated nano-structures in the concentration range of 30–80 mol %. The diameter of one structure was 12–20 nm, dependent on concentration, which is ascribed to cellulose chain entanglement. In contrast, the other with 4.1 nm diameter exhibited concentration independence and is reminiscent of a cellulose microfibril, reflecting the occurrence of nanofibrillation. These results contribute to an understanding of the dissolution mechanism of cellulose in ILs. Finally, we unexpectedly proposed a novel cellulose/IL composite: the cellulose/IL mixtures of 30–50 mol % that possess liquid crystallinity are sufficiently hard to be moldable.
- Published
- 2017
48. Anion Bridging-Induced Structural Transformation of Cellulose Dissolved in Ionic Liquid
- Author
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Kenji Takahashi, Takatsugu Endo, Shunsuke Fujii, Shota Hosomi, and Kazuaki Ninomiya
- Subjects
Inorganic chemistry ,Intercalation (chemistry) ,02 engineering and technology ,Nuclear magnetic resonance spectroscopy ,010402 general chemistry ,021001 nanoscience & nanotechnology ,01 natural sciences ,0104 chemical sciences ,Ion ,chemistry.chemical_compound ,chemistry ,Phase (matter) ,Lyotropic ,Ionic liquid ,Polymer chemistry ,Mole ,General Materials Science ,Physical and Theoretical Chemistry ,Cellulose ,0210 nano-technology - Abstract
We performed structural investigations of cellulose mixed with 1-ethyl-3-methylimidazolium acetate ([Emim][OAc]) in the entire concentration range (0–100 mol %) by wide-angle X-ray scattering with the aid of quantum chemical calculations and 13C solid-state NMR spectroscopy. We particularly focused on a highly concentrated region (≥30 mol %), which has previously been overlooked. At concentrations of 15–30 mol %, a periodic peak corresponding to cellulose chain alignment emerged; this is associated with a lyotropic cholesteric liquid-crystalline phase. At concentrations of ≥30 mol %, the structure is transformed into ordered layers where OAc anions and Emim cations intercalate. This transformation is found to be driven by a change in the interaction between the IL anions and the OH groups of cellulose. At low concentrations, the anion mainly interacts with the OH group of cellulose in a 1:1 ratio, as previously reported; at high concentrations, the anions bridge the OH groups of two cellulose chains.
- Published
- 2016
49. Simultaneous determination of glycyrrhizin and liquiritin in licorice roots and Kampo medicines by combination enzyme-linked immunosorbent assay using anti-glycyrrhizin and anti-liquiritin monoclonal antibodies
- Author
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Takuhiro Uto, Shunsuke Fujii, Yukihiro Shoyama, Osamu Morinaga, and Shuichi Nomura
- Subjects
medicine.drug_class ,Kampo ,Clinical Biochemistry ,Immunology ,Enzyme-Linked Immunosorbent Assay ,Monoclonal antibody ,01 natural sciences ,Plant Roots ,Antigen-Antibody Reactions ,chemistry.chemical_compound ,Glucosides ,medicine ,Glycyrrhiza ,Immunology and Allergy ,Glycyrrhizin ,chemistry.chemical_classification ,Chromatography ,medicine.diagnostic_test ,biology ,010405 organic chemistry ,business.industry ,010401 analytical chemistry ,Antibodies, Monoclonal ,biology.organism_classification ,Glycyrrhizic Acid ,0104 chemical sciences ,Medical Laboratory Technology ,Enzyme ,chemistry ,Licorice roots ,Immunoassay ,Flavanones ,Medicine, Kampo ,business ,Liquiritin - Abstract
Immunoassay systems using monoclonal antibodies (mAbs) are one of the most useful techniques in the analytical, biochemical, and clinical fields. In this study, a combination enzyme-linked immunosorbent assay (ELISA) using both anti-glycyrrhizin and anti-liquiritin mAbs (anti-GL/Liq mixture mAbs) was developed for quality control of licorice and its products. The combination ELISA demonstrated high sensitivity, reproducibility, and specificity for the total content of GL and Liq by a single assay. The developed ELISA was effective and useful as the first screening method in the selection of high-quality licorice from the Glycyrrhiza species and in confirming the quality of licorice-containing Kampo medicines.
- Published
- 2016
50. Liquiritin and Liquiritigenin Induce Melanogenesis via Enhancement of p38 and PKA Signaling Pathways
- Author
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Yukihiro Shoyama, Takuhiro Uto, Akihisa Yamashita, Shunsuke Fujii, and Tomoe Ohta
- Subjects
Tyrosinase ,lcsh:Medicine ,p38 ,tyrosinase ,CREB ,Article ,Melanin ,03 medical and health sciences ,chemistry.chemical_compound ,0302 clinical medicine ,licorice ,PKA ,LY294002 ,Protein kinase A ,Protein kinase B ,030304 developmental biology ,General Environmental Science ,MITF ,0303 health sciences ,integumentary system ,biology ,lcsh:R ,General Engineering ,liquiritin ,Microphthalmia-associated transcription factor ,melanin ,Cell biology ,chemistry ,liquiritigenin ,030220 oncology & carcinogenesis ,TRP-1/2 ,biology.protein ,General Earth and Planetary Sciences ,Liquiritigenin - Abstract
Background: Liquiritin (LQ) and its aglycone, liquiritigenin (LQG), are major flavonoids in licorice root (Glycyrrhiza spp.). Our preliminary screening identified LQ and LQG, which promote melanin synthesis in the melanoma cells. In this study, we investigated the molecular mechanism of melanin synthesis activated by LQ and LQG. Methods: Murine (B16-F1) and human (HMVII) melanoma cell lines were treated with LQ or LQG. After incubation, melanin contents, intracellular tyrosinase activity, and cell viability were evaluated. Protein levels were determined using Western blotting. Results: LQ and LQG activated melanin synthesis and intracellular tyrosinase activity. The induction of melanin and intracellular tyrosinase activity by LQG was higher than that by LQ. LQ and LQG induced the expression of tyrosinase, tyrosinase-related protein (TRP)-1, and TRP-2. LQ and LQG also enhanced microphthalmia-associated transcription factor (MITF) expression, and cyclic AMP-responsive element-binding protein (CREB) phosphorylation. The phosphorylation of p38 and extracellular signal-regulated kinase (ERK), but not Akt, was significantly increased by LQ or LQG. Furthermore, LQ- or LQG-mediated melanin synthesis was partially blocked by p38 inhibitor (SB203580) and protein kinase A (PKA) inhibitor (H-89), however, ERK kinase (MEK) inhibitor (U0126) and phosphatidylinositol-3-kinase (PI3K) inhibitor (LY294002) had no effect. Conclusions: The results suggest that LQ and LQG enhance melanin synthesis by upregulating the expression of melanogenic enzymes, which were activated by p38 and PKA signaling pathways, leading to MITF expression and CREB phosphorylation.
- Published
- 2019
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