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138 results on '"De Giglio, O."'

1. Multi-target assessment of advanced oxidation processes-based strategies for indirect potable reuse of tertiary wastewater: Fate of compounds of emerging concerns, microbial and ecotoxicological parameters

Author

Murgolo, S., De Giglio, O., De Ceglie, C., Triggiano, F., Apollonio, F., Calia, C., Pousis, C., Marzella, A., Fasano, F., Giordano, M.E., Lionetto, M.G., Santoro, D., Santoro, O., Mancini, S., Di Iaconi, C., De Sanctis, M., Montagna, M.T., and Mascolo, G.

Published
2024
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2. The intertwining of healthcare-associated infections and COVID-19 in Italian intensive care units: an analysis of the SPIN-UTI project from 2006 to 2021

Author

Murgia, P., Masia, M.D., Mura, I., Brusaferro, S., Arnoldo, L., Di Stefano, C., Lucchese, F., Lugano, M., Tardivo, S., Moretti, F., Bernasconi, M.O., Pappalardo, F., Pasquarella, C., Sicoli, E., Montagna, M.T., Caggiano, G., De Giglio, O., Fenaroli, S., Squeri, R., Cannavò, G., Pulvirenti, A., Catalano, S., Mattaliano, A.R., Castiglione, G., Astuto, M., La Camera, G., Panascia, E., Longhitano, A.M., Scrofani, G., Gallea, M.R., Civello, P., Milazzo, M., Calamusa, G., Giarratano, A., Di Benedetto, A., Rizzo, G.M.G., Manta, G., Angelone, C., Mancuso, R., Tetamo, R., Mella, L.M., Dei, I., Pandiani, I., Cannistrà, A., Piotti, P., Girardis, M., Barbieri, A., Borracino, S., Palermo, R., Di Stefano, D., Colombo, A., Romeo, A., Minerva, M., Fabiani, L., Marinangeli, F., D’Errico, M.M., Donati, A., Domizi, R., Saglimbene, S.T., Bianco, A., Vittori, C., Orsi, G.B., Scibilia, M., Calà, O., Di Giacinto, I., Amatucci, M.R., Principi, T., Di Fabio, G., Gobbini, V., Olori, M.P., Antonelli, M., Laurenti, P., Condorelli, L., Ingala, F., Russo, S., Costa, P., Canonico, L., Farruggia, P., Cristina, M.L., Sartini, M., Arrigoni, C., Galassi, I., Vinci, V.M., Barchitta, M., Maugeri, A., Favara, G., Lio, R. Magnano San, La Rosa, M.C., D'Ancona, F., and Agodi, A.

Published
2023
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3. Tracking the Spread of the BA.2.86 Lineage in Italy Through Wastewater Analysis

Author

Veneri, C, Brandtner, D, Mancini, P, Bonanno Ferraro, G, Iaconelli, M, Suffredini, E, Petrillo, M, Leoni, G, Paracchini, V, Gawlik, B, Marchini, A, Torlontano, P, Aprea, G, Scattolini, S, Acciari, V, La Bianca, M, Cifarelli, R, Palma, A, Lauria, G, La Vecchia, G, Giordano, V, Cossentino, L, Pennino, F, Lombardi, A, Gentili, L, Angelini, P, Nasci, D, Alborali, G, Formenti, N, Guarneri, F, Fontani, N, Guercio, M, Mariuz, M, Trani, G, Pariani, A, De Lellis, L, Ancona, C, Barca, A, Serio, F, Giorgi, D, Ferrante, I, Capparuccini, V, Scicluna, M, Cersini, A, Pietrella, G, Nicosia, E, Grasselli, E, Izzotti, A, Tomesani, I, Bellisomi, M, Rosatto, S, Ammoni, E, Cereda, D, Bertasi, B, Losio, M, Oliva, D, Castiglioni, S, Schiarea, S, Binda, S, Primache, V, Pellegrinelli, L, Cocuzza, C, Musumeci, R, Bolognini, L, Filippetti, F, Paniccia', M, Briscolini, S, Magi, S, Grucci, A, Colitti, M, Ciccaglione, A, Montanaro, C, Griglio, B, Costa, A, Decastelli, L, Romano, A, Bianchi, M, Carraro, E, Pignata, C, Macrì, M, Bonetta, S, Albano, N, Di Vittorio, G, Mongelli, O, Apollonio, F, Triggiano, F, De Giglio, O, Montagna, M, Palermo, M, Maida, C, Mazzucco, W, Tramuto, F, De Grazia, S, Giammanco, G, Filizzolo, C, Purpari, G, Gucciardi, F, Ferrante, M, Agodi, A, Barchitta, M, Cala', P, Carducci, A, Verani, M, Federigi, I, Macrì, S, Federici, E, Petricciuolo, M, Carnevali, A, Russo, F, Pitter, G, Groppi, V, Rigoli, F, Zampini, M, Baldovin, T, Amoruso, I, Cadonna, M, Postinghel, M, Foladori, P, Zago, L, Stenico, A, Marco, M, Matteo, D, La Rosa, G, Veneri, C., Brandtner, D., Mancini, P., Bonanno Ferraro, G., Iaconelli, M., Suffredini, E., Petrillo, M., Leoni, G., Paracchini, V., Gawlik, B. M., Marchini, A., Torlontano, Paolo, Aprea, Giuseppe, Scattolini, Silvia, Acciari, Vicdalia Aniela, La Bianca, Michele, Cifarelli, Rosa Anna, Palma, Achille, Lauria, Giuseppe, La Vecchia, Giovanna, Giordano, Vincenzo, Cossentino, Luigi, Pennino, Francesca, Lombardi, Annalisa, Gentili, Lisa, Angelini, Paola, Nasci, Daniele, Alborali, Giovanni, Formenti, Nicoletta, Guarneri, Flavia, Fontani, Nadia, Guercio, Marco, Mariuz, Marika, Trani, Gabriella, Pariani, Anna, De Lellis, Laura, Ancona, Carla, Barca, Alessandra, Serio, Flavia, Giorgi, Doriana Antonella, Ferrante, Irene, Capparuccini, Valeria, Scicluna, Maria Teresa, Cersini, Antonella, Pietrella, Gabriele, Nicosia, Elena, Grasselli, Elena, Izzotti, Alberto, Tomesani, Irene, Bellisomi, Marta, Rosatto, Stefano, Ammoni, Emanuela, Cereda, Danilo, Bertasi, Barbara, Losio, Marina Nadia, Oliva, Desdemona, Castiglioni, Sara, Schiarea, Silvia, Binda, Sandro, Primache, Valeria, Pellegrinelli, Laura, Cocuzza, Clementina, Musumeci, Rosario, Bolognini, Luigi, Filippetti, Fabio, Paniccia', Marta, Briscolini, Sara, Magi, Silvia, Grucci, Annalisa, Colitti, Michele, Ciccaglione, Angela, Montanaro, Carmen, Griglio, Bartolomeo, Costa, Angela, Decastelli, Lucia, Romano, Angelo, Bianchi, Manila, Carraro, Elisabetta, Pignata, Cristina, Macrì, Manuela, Bonetta, Silvia, Albano, Nehludoff, Di Vittorio, Giuseppe, Mongelli, Onofrio, Apollonio, Francesca, Triggiano, Francesco, De Giglio, Osvalda, Montagna, Maria Teresa, Palermo, Mario, Maida, Carmelo Massimo, Mazzucco, Walter, Tramuto, Fabio, De Grazia, Simona, Giammanco, Giovanni Maurizio, Filizzolo, Chiara, Purpari, Giuseppa, Gucciardi, Francesca, Ferrante, Margherita, Agodi, Antonella, Barchitta, Martina, Cala', Piergiuseppe, Carducci, Annalaura, Verani, Marco, Federigi, Ileana, Macrì, Salvatore, Federici, Ermanno, Petricciuolo, Maya, Carnevali, Agnese, Russo, Francesca, Pitter, Gisella, Groppi, Vanessa, Rigoli, Franco, Zampini, Marco, Baldovin, Tatjana, Amoruso, Irene, Cadonna, Maria, Postinghel, Mattia, Foladori, Paola, Zago, Lorella, Stenico, Alberta, Marco, Morelli, Matteo, Dossena, La Rosa, G., Veneri, C, Brandtner, D, Mancini, P, Bonanno Ferraro, G, Iaconelli, M, Suffredini, E, Petrillo, M, Leoni, G, Paracchini, V, Gawlik, B, Marchini, A, Torlontano, P, Aprea, G, Scattolini, S, Acciari, V, La Bianca, M, Cifarelli, R, Palma, A, Lauria, G, La Vecchia, G, Giordano, V, Cossentino, L, Pennino, F, Lombardi, A, Gentili, L, Angelini, P, Nasci, D, Alborali, G, Formenti, N, Guarneri, F, Fontani, N, Guercio, M, Mariuz, M, Trani, G, Pariani, A, De Lellis, L, Ancona, C, Barca, A, Serio, F, Giorgi, D, Ferrante, I, Capparuccini, V, Scicluna, M, Cersini, A, Pietrella, G, Nicosia, E, Grasselli, E, Izzotti, A, Tomesani, I, Bellisomi, M, Rosatto, S, Ammoni, E, Cereda, D, Bertasi, B, Losio, M, Oliva, D, Castiglioni, S, Schiarea, S, Binda, S, Primache, V, Pellegrinelli, L, Cocuzza, C, Musumeci, R, Bolognini, L, Filippetti, F, Paniccia', M, Briscolini, S, Magi, S, Grucci, A, Colitti, M, Ciccaglione, A, Montanaro, C, Griglio, B, Costa, A, Decastelli, L, Romano, A, Bianchi, M, Carraro, E, Pignata, C, Macrì, M, Bonetta, S, Albano, N, Di Vittorio, G, Mongelli, O, Apollonio, F, Triggiano, F, De Giglio, O, Montagna, M, Palermo, M, Maida, C, Mazzucco, W, Tramuto, F, De Grazia, S, Giammanco, G, Filizzolo, C, Purpari, G, Gucciardi, F, Ferrante, M, Agodi, A, Barchitta, M, Cala', P, Carducci, A, Verani, M, Federigi, I, Macrì, S, Federici, E, Petricciuolo, M, Carnevali, A, Russo, F, Pitter, G, Groppi, V, Rigoli, F, Zampini, M, Baldovin, T, Amoruso, I, Cadonna, M, Postinghel, M, Foladori, P, Zago, L, Stenico, A, Marco, M, Matteo, D, La Rosa, G, Veneri, C., Brandtner, D., Mancini, P., Bonanno Ferraro, G., Iaconelli, M., Suffredini, E., Petrillo, M., Leoni, G., Paracchini, V., Gawlik, B. M., Marchini, A., Torlontano, Paolo, Aprea, Giuseppe, Scattolini, Silvia, Acciari, Vicdalia Aniela, La Bianca, Michele, Cifarelli, Rosa Anna, Palma, Achille, Lauria, Giuseppe, La Vecchia, Giovanna, Giordano, Vincenzo, Cossentino, Luigi, Pennino, Francesca, Lombardi, Annalisa, Gentili, Lisa, Angelini, Paola, Nasci, Daniele, Alborali, Giovanni, Formenti, Nicoletta, Guarneri, Flavia, Fontani, Nadia, Guercio, Marco, Mariuz, Marika, Trani, Gabriella, Pariani, Anna, De Lellis, Laura, Ancona, Carla, Barca, Alessandra, Serio, Flavia, Giorgi, Doriana Antonella, Ferrante, Irene, Capparuccini, Valeria, Scicluna, Maria Teresa, Cersini, Antonella, Pietrella, Gabriele, Nicosia, Elena, Grasselli, Elena, Izzotti, Alberto, Tomesani, Irene, Bellisomi, Marta, Rosatto, Stefano, Ammoni, Emanuela, Cereda, Danilo, Bertasi, Barbara, Losio, Marina Nadia, Oliva, Desdemona, Castiglioni, Sara, Schiarea, Silvia, Binda, Sandro, Primache, Valeria, Pellegrinelli, Laura, Cocuzza, Clementina, Musumeci, Rosario, Bolognini, Luigi, Filippetti, Fabio, Paniccia', Marta, Briscolini, Sara, Magi, Silvia, Grucci, Annalisa, Colitti, Michele, Ciccaglione, Angela, Montanaro, Carmen, Griglio, Bartolomeo, Costa, Angela, Decastelli, Lucia, Romano, Angelo, Bianchi, Manila, Carraro, Elisabetta, Pignata, Cristina, Macrì, Manuela, Bonetta, Silvia, Albano, Nehludoff, Di Vittorio, Giuseppe, Mongelli, Onofrio, Apollonio, Francesca, Triggiano, Francesco, De Giglio, Osvalda, Montagna, Maria Teresa, Palermo, Mario, Maida, Carmelo Massimo, Mazzucco, Walter, Tramuto, Fabio, De Grazia, Simona, Giammanco, Giovanni Maurizio, Filizzolo, Chiara, Purpari, Giuseppa, Gucciardi, Francesca, Ferrante, Margherita, Agodi, Antonella, Barchitta, Martina, Cala', Piergiuseppe, Carducci, Annalaura, Verani, Marco, Federigi, Ileana, Macrì, Salvatore, Federici, Ermanno, Petricciuolo, Maya, Carnevali, Agnese, Russo, Francesca, Pitter, Gisella, Groppi, Vanessa, Rigoli, Franco, Zampini, Marco, Baldovin, Tatjana, Amoruso, Irene, Cadonna, Maria, Postinghel, Mattia, Foladori, Paola, Zago, Lorella, Stenico, Alberta, Marco, Morelli, Matteo, Dossena, and La Rosa, G.

Abstract

The emergence of new SARS-CoV-2 variants poses challenges to global surveillance efforts, necessitating swift actions in their detection, evaluation, and management. Among the most recent variants, Omicron BA.2.86 and its sub-lineages have gained attention due to their potential immune evasion properties. This study describes the development of a digital PCR assay for the rapid detection of BA.2.86 and its descendant lineages, in wastewater samples. By using this assay, we analyzed wastewater samples collected in Italy from September 2023 to January 2024. Our analysis revealed the presence of BA.2.86 lineages already in October 2023 with a minimal detection rate of 2% which then rapidly increased, becoming dominant by January 2024, accounting for a prevalence of 62%. The findings emphasize the significance of wastewater-based surveillance in tracking emerging variants and underscore the efficacy of targeted digital PCR assays for environmental monitoring.

Published
2024

5. The rapid spread of SARS-COV-2 Omicron variant in Italy reflected early through wastewater surveillance

Author

La Rosa, G, Iaconelli, M, Veneri, C, Mancini, P, Bonanno Ferraro, G, Brandtner, D, Lucentini, L, Bonadonna, L, Rossi, M, Grigioni, M, Suffredini, E, Bucciarelli, G, Torlontano, P, Aprea, G, La Bianca, M, Cifarelli, R, Palma, A, La Vecchia, G, Lauria, G, Brienza, R, Montenegro, P, D'Argenzio, A, Cossentino, L, Olivares, R, Pizzolante, A, Fusco, G, Tosco, A, Porta, A, Pennino, F, Maria, T, Angelini, P, De Lellis, L, Nasci, D, Alborali, G, Formenti, N, Guarneri, F, Fontani, N, Nani, G, Palumbo, F, Borlone, G, Guercio, M, Gentili, L, Mariuz, M, Trani, G, Pariani, A, Ancona, C, Giorgi, D, Ferrante, I, Monfrinotti, M, Riosa, S, Capparuccini, V, Scicluna, M, Cersini, A, Arizzi, M, Cecchini, G, Ottaviano, C, Nicosia, E, Grasselli, E, Allaria, G, Izzotti, A, Rosatto, S, Ammoni, E, Cereda, D, Losio, M, Bertasi, B, Aliscioni, A, Oliva, D, Castiglioni, S, Schiarea, S, Zuccato, E, Antonelli, M, Azzellino, A, Malpei, F, Turolla, A, Binda, S, Laura, P, Primache, V, Cocuzza, C, Franzetti, A, Bertanza, G, Callegari, M, Bolognini, L, Filippetti, F, Paniccia', M, Ciuti, F, Briscolini, S, Magi, S, Colitti, M, Montanaro, C, Cerroni, M, Griglio, B, Berruti, R, Cravero, M, Costa, A, Bianchi, M, Decastelli, L, Romano, A, Zuccon, F, Carraro, E, Pignata, C, Bonetta, S, Di Vittorio, G, Mongelli, O, De Giglio, O, Apollonio, F, Triggiano, F, Montagna, M, Ungaro, N, Palermo, M, Maida, C, Mazzucco, W, De Grazia, S, Giammanco, G, Purpari, G, Ferrante, M, Agodi, A, Barchitta, M, Cala', P, Carducci, A, Verani, M, Federigi, I, Lauretani, G, Muzio, S, Ramazzotti, M, Antonelli, A, Ricci, E, Santoro, G, Federici, E, Petricciuolo, M, Barigelli, S, Ruffier, M, Borney, F, Grange, E, Damasco, F, Russo, F, Pitter, G, Groppi, V, Rigoli, F, Zampini, M, Baldovin, T, Amoruso, I, Mengon, E, Cadonna, M, Postinghel, M, Pizzo, F, Schiavuzzi, A, Cutrupi, F, Foladori, P, Manara, S, Zago, L, Stenico, A, Prast, A, La Rosa G., Iaconelli M., Veneri C., Mancini P., Bonanno Ferraro G., Brandtner D., Lucentini L., Bonadonna L., Rossi M., Grigioni M., Suffredini E., Bucciarelli G., Torlontano P., Aprea G., La Bianca M., Cifarelli R. A., Palma A., La Vecchia G., Lauria G., Brienza R., Montenegro P., D'Argenzio A., Cossentino L., Olivares R., Pizzolante A., Fusco G., Tosco A., Porta A., Pennino F., Maria T., Angelini P., De Lellis L., Nasci D., Alborali G., Formenti N., Guarneri F., Fontani N., Nani G., Palumbo F., Borlone G., Guercio M., Gentili L., Mariuz M., Trani G., Pariani A., Ancona C., Giorgi D. A., Ferrante I., Monfrinotti M., Riosa S., Capparuccini V., Scicluna M. T., Cersini A., Arizzi M., Cecchini G., Ottaviano C., Nicosia E., Grasselli E., Allaria G., Izzotti A., Rosatto S., Ammoni E., Cereda D., Losio M. N., Bertasi B., Aliscioni A., Oliva D., Castiglioni S., Schiarea S., Zuccato E., Antonelli M., Azzellino A., Malpei F., Turolla A., Binda S., Laura P., Primache V., Cocuzza C., Franzetti A., Bertanza G., Callegari M. L., Bolognini L., Filippetti F., Paniccia' M., Ciuti F., Briscolini S., Magi S., Colitti M., Montanaro C., Cerroni M. G., Griglio B., Berruti R., Cravero M., Costa A., Bianchi M., Decastelli L., Romano A., Zuccon F., Carraro E., Pignata C., Bonetta S., Di Vittorio G., Mongelli O., De Giglio O., Apollonio F., Triggiano F., Montagna M. T., Ungaro N., Palermo M., Maida C. M., Mazzucco W., De Grazia S., Giammanco G., Purpari G., Ferrante M., Agodi A., Barchitta M., Cala' P., Carducci A., Verani M., Federigi I., Lauretani G., Muzio S., Ramazzotti M., Antonelli A., Ricci E., Santoro G., Federici E., Petricciuolo M., Barigelli S., Ruffier M., Borney F., Grange E., Damasco F., Russo F., Pitter G., Groppi V., Rigoli F., Zampini M., Baldovin T., Amoruso I., Mengon E., Cadonna M., Postinghel M., Pizzo F., Schiavuzzi A., Cutrupi F., Foladori P., Manara S., Zago L., Stenico A., Prast A. -M., La Rosa, G, Iaconelli, M, Veneri, C, Mancini, P, Bonanno Ferraro, G, Brandtner, D, Lucentini, L, Bonadonna, L, Rossi, M, Grigioni, M, Suffredini, E, Bucciarelli, G, Torlontano, P, Aprea, G, La Bianca, M, Cifarelli, R, Palma, A, La Vecchia, G, Lauria, G, Brienza, R, Montenegro, P, D'Argenzio, A, Cossentino, L, Olivares, R, Pizzolante, A, Fusco, G, Tosco, A, Porta, A, Pennino, F, Maria, T, Angelini, P, De Lellis, L, Nasci, D, Alborali, G, Formenti, N, Guarneri, F, Fontani, N, Nani, G, Palumbo, F, Borlone, G, Guercio, M, Gentili, L, Mariuz, M, Trani, G, Pariani, A, Ancona, C, Giorgi, D, Ferrante, I, Monfrinotti, M, Riosa, S, Capparuccini, V, Scicluna, M, Cersini, A, Arizzi, M, Cecchini, G, Ottaviano, C, Nicosia, E, Grasselli, E, Allaria, G, Izzotti, A, Rosatto, S, Ammoni, E, Cereda, D, Losio, M, Bertasi, B, Aliscioni, A, Oliva, D, Castiglioni, S, Schiarea, S, Zuccato, E, Antonelli, M, Azzellino, A, Malpei, F, Turolla, A, Binda, S, Laura, P, Primache, V, Cocuzza, C, Franzetti, A, Bertanza, G, Callegari, M, Bolognini, L, Filippetti, F, Paniccia', M, Ciuti, F, Briscolini, S, Magi, S, Colitti, M, Montanaro, C, Cerroni, M, Griglio, B, Berruti, R, Cravero, M, Costa, A, Bianchi, M, Decastelli, L, Romano, A, Zuccon, F, Carraro, E, Pignata, C, Bonetta, S, Di Vittorio, G, Mongelli, O, De Giglio, O, Apollonio, F, Triggiano, F, Montagna, M, Ungaro, N, Palermo, M, Maida, C, Mazzucco, W, De Grazia, S, Giammanco, G, Purpari, G, Ferrante, M, Agodi, A, Barchitta, M, Cala', P, Carducci, A, Verani, M, Federigi, I, Lauretani, G, Muzio, S, Ramazzotti, M, Antonelli, A, Ricci, E, Santoro, G, Federici, E, Petricciuolo, M, Barigelli, S, Ruffier, M, Borney, F, Grange, E, Damasco, F, Russo, F, Pitter, G, Groppi, V, Rigoli, F, Zampini, M, Baldovin, T, Amoruso, I, Mengon, E, Cadonna, M, Postinghel, M, Pizzo, F, Schiavuzzi, A, Cutrupi, F, Foladori, P, Manara, S, Zago, L, Stenico, A, Prast, A, La Rosa G., Iaconelli M., Veneri C., Mancini P., Bonanno Ferraro G., Brandtner D., Lucentini L., Bonadonna L., Rossi M., Grigioni M., Suffredini E., Bucciarelli G., Torlontano P., Aprea G., La Bianca M., Cifarelli R. A., Palma A., La Vecchia G., Lauria G., Brienza R., Montenegro P., D'Argenzio A., Cossentino L., Olivares R., Pizzolante A., Fusco G., Tosco A., Porta A., Pennino F., Maria T., Angelini P., De Lellis L., Nasci D., Alborali G., Formenti N., Guarneri F., Fontani N., Nani G., Palumbo F., Borlone G., Guercio M., Gentili L., Mariuz M., Trani G., Pariani A., Ancona C., Giorgi D. A., Ferrante I., Monfrinotti M., Riosa S., Capparuccini V., Scicluna M. T., Cersini A., Arizzi M., Cecchini G., Ottaviano C., Nicosia E., Grasselli E., Allaria G., Izzotti A., Rosatto S., Ammoni E., Cereda D., Losio M. N., Bertasi B., Aliscioni A., Oliva D., Castiglioni S., Schiarea S., Zuccato E., Antonelli M., Azzellino A., Malpei F., Turolla A., Binda S., Laura P., Primache V., Cocuzza C., Franzetti A., Bertanza G., Callegari M. L., Bolognini L., Filippetti F., Paniccia' M., Ciuti F., Briscolini S., Magi S., Colitti M., Montanaro C., Cerroni M. G., Griglio B., Berruti R., Cravero M., Costa A., Bianchi M., Decastelli L., Romano A., Zuccon F., Carraro E., Pignata C., Bonetta S., Di Vittorio G., Mongelli O., De Giglio O., Apollonio F., Triggiano F., Montagna M. T., Ungaro N., Palermo M., Maida C. M., Mazzucco W., De Grazia S., Giammanco G., Purpari G., Ferrante M., Agodi A., Barchitta M., Cala' P., Carducci A., Verani M., Federigi I., Lauretani G., Muzio S., Ramazzotti M., Antonelli A., Ricci E., Santoro G., Federici E., Petricciuolo M., Barigelli S., Ruffier M., Borney F., Grange E., Damasco F., Russo F., Pitter G., Groppi V., Rigoli F., Zampini M., Baldovin T., Amoruso I., Mengon E., Cadonna M., Postinghel M., Pizzo F., Schiavuzzi A., Cutrupi F., Foladori P., Manara S., Zago L., Stenico A., and Prast A. -M.

Abstract

The SARS-CoV-2 Omicron variant emerged in South Africa in November 2021, and has later been identified worldwide, raising serious concerns. A real-time RT-PCR assay was designed for the rapid screening of the Omicron variant, targeting characteristic mutations of the spike gene. The assay was used to test 737 sewage samples collected throughout Italy (19/21 Regions) between 11 November and 25 December 2021, with the aim of assessing the spread of the Omicron variant in the country. Positive samples were also tested with a real-time RT-PCR developed by the European Commission, Joint Research Centre (JRC), and through nested RT-PCR followed by Sanger sequencing. Overall, 115 samples tested positive for Omicron SARS-CoV-2 variant. The first occurrence was detected on 7 December, in Veneto, North Italy. Later on, the variant spread extremely fast in three weeks, with prevalence of positive wastewater samples rising from 1.0% (1/104 samples) in the week 5–11 December, to 17.5% (25/143 samples) in the week 12–18, to 65.9% (89/135 samples) in the week 19–25, in line with the increase in cases of infection with the Omicron variant observed during December in Italy. Similarly, the number of Regions/Autonomous Provinces in which the variant was detected increased from one in the first week, to 11 in the second, and to 17 in the last one. The presence of the Omicron variant was confirmed by the JRC real-time RT-PCR in 79.1% (91/115) of the positive samples, and by Sanger sequencing in 66% (64/97) of PCR amplicons. In conclusion, we designed an RT-qPCR assay capable to detect the Omicron variant, which can be successfully used for the purpose of wastewater-based epidemiology. We also described the history of the introduction and diffusion of the Omicron variant in the Italian population and territory, confirming the effectiveness of sewage monitoring as a powerful surveillance tool.

Published
2022

6. The role of air temperature in Legionella water contamination and legionellosis incidence rates in southern Italy (2018-2023).

Author

Montagna, M. T., Brigida, S., Fasano, F., Leone, C. M., D'Ambrosio, M., Spagnuolo, V., Lopuzzo, M., Apollonio, F., Triggiano, F., Caringella, M. E., and De Giglio, O.

Subjects
ATMOSPHERIC temperature, WATER pollution, LEGIONNAIRES' disease
Abstract

Copyright of Annali di Igiene, Medicina Preventiva e di Comunità is the property of Societa Editrice Universo s.r.l. and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Published
2023
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8. Resistenze antibiotiche in acque adibite al consumo umano: diffusione e potenziale impatto sulla salute

Author

Federigi, I., Tesauro, M., Bagordo, F., Bonetta, S., Consonni, M., OLIVERI CONTI, G., DE GIGLIO, O., Diella, G., Ferrante, M., Grasso, A., Macrì, M., Montagna, M.T., Verani, M., and Carducci, A.

Subjects
AMR, tap water, Resistenze antibiotiche, acque per il consumo umano, rischi, Settore MED/42 - Igiene Generale e Applicata, Resistenze antibiotiche, acque per il consumo umano, rischi
Published
2022

9. A multicentre study on epidemiology and prevention of needle stick injuries among students of nursing schools

Author

Veronesi, L, Giudice, L, Agodi, A, Arrigoni, C, Baldovin, T, Barchitta, M, Benedetti, T, Caggiano, G, Cannizzaro, S G, De Giglio, O, D'Errico, M, Destri, S, Fiorentini, R, Gentile, L, Mannone, A, Mascipinto, S, Mercuri, M, Montagna, M T, Novati, R, Oriani, R, Ortolani, S, Pennino, F, Ripabelli, G, Rossini, A, Sammarco, M L, Sodano, L, Squeri, R, Tamarri, F, Tamburro, M, Torre, I, Troiani, S, Pasquarella, C, Veronesi, L, Giudice, L, Agodi, A, Arrigoni, C, Baldovin, T, Barchitta, M, Benedetti, T, Caggiano, G, Cannizzaro, SUSANNA GAIA, De Giglio, O, D'Errico, M, Destri, S, Fiorentini, R, Gentile, L, Mannone, A, Mascipinto, S, Mercuri, M, Montagna, Mt, Novati, R, Oriani, R, Ortolani, S, Pennino, F, Ripabelli, G, Rossini, A, Sammarco, Ml, Sodano, L, Squeri, R, Tamarri, F, Tamburro, M, Torre, I, Troiani, S, Pasquarella, C., Veronesi, L., Giudice, L., Agodi, A., Arrigoni, C., Baldovin, T., Barchitta, M., Benedetti, T., Caggiano, G., Cannizzaro, S. G., De Giglio, O., D'Errico, M., Destri, S., Fiorentini, R., Gentile, L., Mannone, A., Mascipinto, S., Mercuri, M., Montagna, M. T., Novati, R., Oriani, R., Ortolani, S., Pennino, F., Ripabelli, G., Rossini, A., Sammarco, M. L., Sodano, L., Squeri, R., Tamarri, F., Tamburro, M., Torre, I., and Troiani, S.

Subjects
Adult, Male, Schools, Nursing, Biological risk, Education, Internship, Needle stick injuries, Training, Nursing, Young Adult, Humans, Infortunio, Sex Distribution, Needlestick Injuries, Students, Schools, Formazione, Punture da ago, Rischio biologico, Tirocinio, Albania, Female, Internship and Residency, Italy, Middle Aged, Post-Exposure Prophylaxis, Students, Nursing, Environmental and Occupational Health, Infectious Diseases, Public Health, Environmental and Occupational Health, Needle stick injurie, Public Health
Abstract

Introduction. Among the health professions with a long period of training, the students of the Nursing Bachelor's Degree are the most exposed to biological risk resulting from accidents, in particular with needles and cutting edges. The aim of the study was to estimate the frequency and the circumstances for the occurrence of needle stick injuries, as a knowledge base for targeted prevention interventions. Methods. The study was carried out between May and July 2017 in 11 Universities in Italy and 1 in Albania (associated with the "Tor Vergata" University of Rome). An anonymous semi-structured questionnaire was proposed to 1st (second semester), 2nd and 3rd year students of Nursing Bachelor's Degree. Results. A total of 2742 questionnaires were collected. The average age of participants was 22.9 years (median 22, range 19-60 years), 73% of whom were females. A total of 381 injuries were reported. Three hundred and sixteen students (11.8%) underwent at least 1 injury (12.7% among females, 9.7% among males); 41 students declared two or more injuries; four students did not report the number of injuries occurred. The first injury occurred, as an average, 17 days after the start of the internship (median 15 days) and, in 25% of the cases, during the first 9 days. The highest percentage of accidents occurred during the first internship (25.3% of the total) and decreased with the progress of the training path. The injuries occurred in 38% of cases during drug preparation, 24% when disposing of sharp devices, 15% while re capping needles, 13% during blood sampling and 10% in other circumstances. In 51.2% of cases, the needle was not sterile. Among the nursing students who suffered a needle stick injury, 58.1% declared that they had performed the post-exposure prophylaxis. 96% of students stated to be vaccinated against Hepatitis B virus. Amongst the students who had their serological status checked (74%), 18% stated the antibody titre was not protective. 49.8% of students answered to have been trained in advance on the correct procedures to avoid needle stick and cutting edges injuries in each clinical ward attended, 41.2% referred that this occurred only in some wards and 10% in no ward at all. Conclusions. The results of this study show a high percentage of needle stick injuries in students of the Nursing Bachelor's Degree. Therefore, there is a need for careful reflection on the most effective methods of targeted training acquisition of knowledge, skills and behavioural models useful for the exercise of the profession.

Published
2018

10. Impact of the environment on the health: From theory to practice

Author

Carducci, A.L., primary, Agodi, A., additional, Ancona, C., additional, Angelini, P., additional, Bagordo, F., additional, Barbone, F., additional, Birbaum, L., additional, Carreri, V., additional, Casuccio, A., additional, Conti, A., additional, Conversano, M., additional, De Donno, A., additional, De Giglio, O., additional, Desiante, F., additional, Di Pietro, A., additional, Dogliotti, E., additional, Donato, F., additional, Fara, G.M., additional, Fiore, M., additional, Forastiere, F., additional, Giammanco, G., additional, Izzotti, A., additional, Montagna, M.T., additional, Oliveri Conti, G., additional, Petronio, M.G., additional, Sciacca, S., additional, Signorelli, C., additional, Testai, E., additional, Verani, M., additional, Vinceti, M., additional, Vitale, F., additional, Ferrante, M., additional, Adani, G., additional, Berghella, L., additional, Calia, C., additional, Calzolari, R., additional, Canale, A., additional, Castiglione, D., additional, Copat, C., additional, Cristaldi, A., additional, Cuffari, G., additional, Coronel Vargas, G., additional, De Vita, E., additional, De Nard, F., additional, Federigi, I., additional, Filippini, T., additional, Grasso, A., additional, Leonardi, N., additional, Letzgus, M., additional, Lo Bianco, G., additional, Mazzucco, W., additional, Nicolosi, I., additional, Orlandi, P., additional, Paladino, G., additional, Pizzo, S., additional, Pousis, C., additional, Raffo, M., additional, Rivolta, S., additional, Scarpitta, F., additional, Trani, G., additional, Triggiano, F., additional, Tumbarello, A., additional, Vecchio, V., additional, Zuccarello, P., additional, and Vassallo, M., additional

Published
2021
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11. SARS-CoV-2 indoor contamination: considerations on anti-COVID-19 management of ventilation systems, and finishing materials in healthcare facilities

Author

Gola, M, Caggiano, G, De Giglio, O, Napoli, C, Diella, G, Carlucci, M, Carpagnano, L F, D'Alessandro, D, Joppolo, C M, Capolongo, S, and Montagna, M T

Subjects
Cross Infection, Best practices, Construction Materials, SARS-CoV-2, Air Microbiology, COVID-19, Equipment Design, HVAC (heating, Risk Assessment, Ventilation, Management, Heating, Healthcare facilities, Air Pollution, Indoor, Equipment Contamination, Humans, Air Conditioning, Hospital Design and Construction, Particulate Matter, Health Facilities, ventilation and air conditioning) and ventilation systems, HVAC (heating, ventilation and air conditioning) and ventilation systems, Pandemics, best practices, healthcare facilities, management, Filtration
Abstract

Many of the devastating pandemics and outbreaks of last centuries have been caused by enveloped viruses. The recent pandemic of Coronavirus disease 2019 (COVID-19) has seriously endangered the global health system. In particular, hospitals have had to deal with a frequency in the emergency room and a request for beds for infectious diseases never faced in the last decades. It is well-known that hospitals are environments with a high infectious risk. Environmental control of indoor air and surfaces becomes an important means of limiting the spread of SARS-CoV-2. In particular, to preserve an adequate indoor microbiological quality, an important non-pharmacological strategy is represented by Heating, Ventilation and Air Conditioning (HVAC) systems and finishing materials. Starting from the SARS-CoV-2 transmission routes, the paper investigates the hospital risk analysis and management, the indoor air quality and determination of microbial load, surface management and strategies in cleaning activities, HVAC systems' management and filters' efficiency. In conclusion, the paper suggests some strategies of interventions and best practices to be taken into considerations for the next steps in design and management.

Published
2020

12. Microbiological and chemical assessment of wastewater discharged by infiltration trenches in fractured and karstified limestone (Sca.re.s. project)

Author

Montagna M.T., De Giglio O., Calia C., Pousis C., Triggiano F., Murgolo S., De Ceglie C., Bagordo F., Apollonio F., Diella G., Narracci M., Acquaviva M.I., Ferraro G.B., Mancini P., Veneri C., Brigida S., Grassi T., De Donno A., Di Iaconi C., Caputo M.C., Cavallo R.A., La Rosa G., and Mascolo G.

Subjects
wastewater, groundwater, bacteria, virus, contaminants of emerging concern
Abstract

This study investigated the environmental contamination of groundwater as a consequence of the discharge of treated wastewater into the soil. The investigation focused on a wastewater treatment plant located in an area fractured by karst in the Salento peninsula (Apulia, Italy). Water samples were collected at four sites (raw wastewater, treated wastewater, infiltration trench, and monitoring well), monthly from May to December 2019 (with the exception of August), and were tested for (1) panel of bacteria; (2) enteric viruses; and (3) chemical substances. A gradual reduction in the concentration of bacteria, viruses and contaminants of emerging concern was observed across the profile of soil fissured by karst. All monitored bacteria were absent from the monitoring well, except for Pseudomonas aeruginosa. Pepper mild mottle virus and adenovirus were detected at all sampling sites. Personal care products and X-ray contrast media showed the greatest decrease in concentration from infiltration trench to the monitoring well, while the highest residual concentrations in the monitoring well were found for anticonvulsants (78.5%), antimicrobials (41.3%), and antipsychotic drugs (38.6%). Our results show that parameters provided by current law may not always be sufficient to evaluate the sanitary risk relating to the discharge of treated wastewater to the soil.

Published
2020
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14. Hospital hygiene in Italy: the GISIO-SItI survey

Author

Pasquarella, C, Ciorba, V, Arnoldo, L, Auxilia, F, Baldovin, T, Barchitta, M, Bianco, A, Calagreti, G, Casini, B, Cristina, M L, De Giglio, O, D'Errico, M M, Fabiani, L, Laganà, P, Laurenti, P, Mattaliano, A R, Molino, A, Montagna, M T, Moscato, U, Mura, I, Napoli, C, Novati, R, Orsi, G B, Privitera, G, Ripabelli, G, Rossini, A, Sodano, L, Squeri, R, Tardivo, S, Teti, V, Torregrossa, M V, Torri, E, Vantaggiato, M D, Veronesi, L, Zarrilli, R, Agodi, A, Brusaferro, S, Pasquarella, C, Ciorba, V, Arnoldo, L, Auxilia, F, Baldovin, T, Barchitta, M, Bianco, A, Calagreti, G, Casini, B, Cristina, Ml, De Giglio, O, D'Errico, Mm, Fabiani, L, Laganà, P, Laurenti, P, Mattaliano, Ar, Molino, A, Montagna, Mt, Moscato, U, Mura, I, Napoli, C, Novati, R, Orsi, Gb, Privitera, G, Ripabelli, G, Rossini, A, Sodano, L, Squeri, R, Tardivo, S, Teti, V, Torregrossa, Mv, Torri, E, Vantaggiato, Md, Veronesi, L, Zarrilli, R, Agodi, A, and Brusaferro, S.

Subjects
Hospital hygiene, questionnaire, survey, Cross Infection, Hospitals, Humans, Infection Control, Italy, Societies, Medical, Hospital Administration, Hygiene, Surveys and Questionnaires, Questionnaire, Environmental and Occupational Health, Hospital hygiene, Questionnaire, Survey, Public Health, Environmental and Occupational Health, Infectious Diseases, Infectious Diseases, Medical, Survey, Public Health, Societies, Public Health, Environmental and Occupational Health, Hospital hygiene, Questionnaire, Survey
Abstract

In Italy there are no rules concerning the establishment of a hospital hygiene structure in hospitals and other healthcare settings, and the hospital organization plans vary widely. The aim of the survey, carried out by the Italian Study Group of Hospital Hygiene of the Italian Society of Hygiene, Preventive medicine and Public health, was to evaluate the presence in the hospital organization plan of a structure referred to as Hospital hygiene, or including in its denomination the words "hygiene" or "hospital hygiene", the activities carried out, the relation to other areas, like patient safety, the type and quantity of professionals involved, the strengths and the critical aspects.A semi-structured questionnaire was administered to Healthcare Trusts representing all Italian Regions through the members of the above Study Group.35 Trusts, 13 in Northern, 8 in Central, 14 in Southern Italy (including Sicily and Sardinia), completed the questionnaire. In 19 Trusts (54.3%) a structure whose denomination included the words "hospital hygiene" or "hygiene" was present. The activities related to the management of infectious risk were most represented, carried out autonomously or in collaboration, but many other activities were covered. In all hospitals the activities of the Hospital Hygiene Unit inter-linked with those of the clinical risk, with different forms of collaboration.This survey, even though on a limited sample, provided a picture of hospital hygiene at a national level, showing a considerable heterogeneity and highlighting critical issues but also strengths. It is essential to share organizational and management models that enhance and promote hospital hygiene, to ensure the appropriateness of healthcare practices offered in a safe and comfortable environment to patients, operators, and visitors.

Published
2018

15. Hospital Hygiene and Infection Prevention and Control in Italy: state of the art and perspectives

Author

Brusaferro, S, Arnoldo, L, Finzi, G, Mura, I, Auxilia, F, Pasquarella, C, Agodi, A and the GISIO Group (Agodi A, Arrigoni, C, Barchitta, M, Calella, G, Casini, B, Cristina, Ml, D'Errico, M, Laurenti, P, Masia, Md, Montagna, Mt, Olivieri, G, Orsi, A, Orsi, Gb, Pesapane, L, Ripabelli, G, Sodano, L, Squeri, R, Teti, V, Torregrossa, Mv, Torri, E, Zarrilli, R, Are, Bm, Brighenti, A, Mascipinto, S, Iannazzo, S, D'Ancona, Fp, Sessa, G, Motta, A, Appignanesi, R, Argiolas, F, Baldovin, T, Bargellini, A, Berdini, S, Boccia, G, Calagreti, G, Caldarulo, T, Campanella, F, Chiesa, R, Ciorba, V, Contrisciani, R, D'Alessandro, D, De Giglio, O, Fabiani, L, Fara, Gm, Giuliani, G, Laganà, P, Marani, A, Mattaliano, Ar, Molino, A, Montesano, M, Moretti, F, Moro, M, Moscato, U, Napoli, C, Nicolotti, N, Nobile, M, Novati, R, Palumbo, F, Piana, A, Privitera, G, Prospero, E, Quattrocchi, A, Righi, E, Romano Spica, V, Rossi, F, Rossini, A, Schieppati, S, Sotgiu, G, Tardivo, S, Torre, I, Valeriani, F, Veronesi, L, Zotti, C), Brusaferro, S., Arnoldo, L., Finzi, G., Mura, I., Auxilia, F., Pasquarella, C., Agodi, A., Pitzurra, M., Arrigoni, C., Barchitta, M., Calella, G., Casini, B., Cristina, M. L., D'Errico, M., Laurenti, P., Masia, M. D., Montagna, M. T., Olivieri, G., Orsi, A., Orsi, G. B., Pesapane, L., Ripabelli, G., Sodano, L., Squeri, R., Teti, V., Torregrossa, M. V., Torri, E., Zarrilli, R., Are, B. M., Brighenti, A., Mascipinto, S., Iannazzo, S., D'Ancona, F. P., Sessa, G., Motta, A., Appignanesi, R., Argiolas, F., Baldovin, T., Bargellini, A., Berdini, S., Boccia, G., Calagreti, G., Caldarulo, T., Campanella, F., Chiesa, R., Ciorba, V., Contrisciani, R., D'Alessandro, D., De Giglio, O., Fabiani, L., Fara, G. M., Giuliani, G., Lagana, P., Marani, A., Mattaliano, A. R., Molino, A., Montesano, M., Moretti, F., Moro, M., Moscato, U., Napoli, C., Nicolotti, N., Nobile, M., Novati, R., Palumbo, F., Piana, A., Privitera, G., Prospero, E., Quattrocchi, A., Righi, E., Romano Spica, V., Rossi, F., Rossini, A., Schieppati, S., Sotgiu, G., Tardivo, S., Torre, I., Valeriani, F., Veronesi, L., and Zotti, C.

Subjects
Adult, Male, Healthcare organisation Igiene ospedaliera, National Health Programs, Infection Prevention and Control, hospital hygiene, infection prevention and control, public health, environmental and occupational health, infectious diseases, igiene ospedaliera, organizzazione sanitaria, prevenzione e controllo delle infezioni, Attitude of Health Personnel, Health Promotion, Healthcare organisation, Iran, Nursing Staff, Hospital, Hospital Hygiene, Igiene ospedaliera, Hospital Hygiene, Infection Prevention and Control, Healthcare organisation Igiene ospedaliera, prevenzione e controllo delle infezioni, organizzazione sanitaria, Hospital Administration, Patient Education as Topic, Terminology as Topic, Medical Staff, Hospital, Humans, Igiene ospedaliera, prevenzione e controllo delle infezioni, organizzazione sanitaria, European Union, Hospitals, Teaching, Workplace, Occupational Health, Cross-Sectional Studie, Cross Infection, Infection Control, Healthcare organisation Parole chiave: Igiene ospedaliera, Analysis of Variance, Organizzazione sanitaria, Prevenzione e controllo delle infezioni, Europe, Italy, Health Facilities, Hygiene, Healthcare organisation, Igiene ospedaliera, Environmental and Occupational Health, Public Health, Environmental and Occupational Health, Infectious Diseases, Healthcare organisation, Hospital Hygiene, Infection Prevention and Control, Public Health, Environmental and Occupational Health, Infectious Diseases, Hospital Administrator, Female, Public Health, Patient Participation, Hospital Hygiene, Infection Prevention and Control, Healthcare organisation, Human
Abstract

Although hospital hygiene has a long history in Italy it is necessary to reflect about it because of the innovation in healthcare systems and because of the evolution due to European Union related activities. Different traditions exist in European countries about hospital hygiene and European Centre for Disease Prevention and Control (ECDC) adopted the term of "infection control and hospital hygiene" which includes all the engaged European healthcare professionals. We propose to modify hospital hygiene as "healthcare organisation hygiene" in order to focalise the attention to all care settings not only hospitals and to adopt the following definition: "all activities aimed to guarantee, in an appropriate, scientifically sound and efficient way, that structures and processes support healthcare practices in a safe comfortable and healthy environment both for patients, caregivers and healthcare workers". Hospital hygiene and its professionals, besides the long tradition, still remain a relevant pillar in guaranteeing quality and safety of healthcare in Italy.

Published
2018

16. Legionella in tourist facilities water system of a South Italy province

Author

Verani, M., Ciniero, A., DE GIGLIO, O., Federigi, I., Favale, I., Spartera, M., Cianciaruso, G., Ragone, M., Aiello, C., Ranieri, S., Conversano, M., Cipriani, R., Rizzi, R., Lucariello, A., Terrone, N., Turco, M. R., Palumbo, N., Montagna, M. T., and Carducci, A

Published
2019

17. Water safety in healthcare facilities. The Vieste Charter

Author

Bonadonna, L., de Grazia, M. C., Capolongo, S., Casini, B., Cristina, M. L., Daniele, G., D'Alessandro, D., De Giglio, O., Di Benedetto, A., Di Vittorio, G., Ferretti, E., Frascolla, B., La Rosa, G., La Sala, L., Lopuzzo, M. G., Lucentini, L., Montagna, M. T., Moscato, U., Pasquarella, C., Prencipe, R., Ricci, M. L., Spica, V. R., Signorelli, C., Veschetti, E., Bonadonna, L., de Grazia, M. C., Capolongo, S., Casini, B., Cristina, M. L., Daniele, G., D'Alessandro, D., De Giglio, O., Di Benedetto, A., Di Vittorio, G., Ferretti, E., Frascolla, B., La Rosa, G., La Sala, L., Lopuzzo, M. G., Lucentini, L., Montagna, M. T., Moscato, U., Pasquarella, C., Prencipe, R., Ricci, M. L., Spica, V. R., Signorelli, C., and Veschetti, E.

Subjects
healthcare facilities, water safety plan, microbiology, Environmental and Occupational Health, Healthcare facilities, Water safety plan, Health Promotion, Healthcare facilities, Water safety plan, Public Health, Environmental and Occupational Health, Infectious Diseases, Water Purification, Infectious Diseases, Italy, Risk Factors, Water Supply, Humans, Health Facilities, Public Health, Safety, Water Microbiology
Abstract

The Study Group on Hospital Hygiene of the Italian Society of Hygiene, Preventive Medicine and Public Health (GISIO-SItI) and the Local Health Authority of Foggia, Apulia, Italy, after the National Convention "Safe water in healthcare facilities" held in Vieste-Pugnochiuso on 27-28 May 2016, present the "Vieste Charter", drawn up in collaboration with experts from the National Institute of Health and the Ministry of Health. This paper considers the risk factors that may affect the water safety in healthcare facilities and reports the current regulatory frameworks governing the management of installations and the quality of the water. The Authors promote a careful analysis of the risks that characterize the health facilities, for the control of which specific actions are recommended in various areas, including water safety plans; approval of treatments; healthcare facilities responsibility, installation and maintenance of facilities; multidisciplinary approach; education and research; regional and national coordination; communication.

Published
2017

18. SARS-CoV-2 indoor contamination: considerations on anti-COVID-19 management of ventilation systems, and finishing materials in healthcare facilities.

Author

Gola, M., Caggiano, G., De Giglio, O., Napoli, C., Diella, G., Carlucci, M., Carpagnano, L. F., D'Alessandro, D., Joppolo, C. M., Capolongo, S., and Montagna, M. T.

Subjects
COVID-19 pandemic, VENTILATION, MEDICAL care, MICROBIAL cells, HOSPITALS
Abstract

Copyright of Annali di Igiene, Medicina Preventiva e di Comunità is the property of Societa Editrice Universo s.r.l. and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Published
2021
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19. Molecular identification and echinocandin susceptibility of candida parapsilosis complex bloodstream isolates in Italy, 2007-2014

Author

Lovero, G, Borghi, E, Balbino, S, Cirasola, D, De Giglio, O, Perdoni, F, Caggiano, G, Morace, G, Montagna, M, Lovero G., Borghi E., Balbino S., Cirasola D., De Giglio O., Perdoni F., Caggiano G., Morace G., Montagna M. T., Lovero, G, Borghi, E, Balbino, S, Cirasola, D, De Giglio, O, Perdoni, F, Caggiano, G, Morace, G, Montagna, M, Lovero G., Borghi E., Balbino S., Cirasola D., De Giglio O., Perdoni F., Caggiano G., Morace G., and Montagna M. T.

Abstract

The Candida parapsilosis group encompasses three species: C. parapsilosis, C. orthopsilosis, and C. metapsilosis. Here, we describe the incidence and echinocandin susceptibility profile of bloodstream isolates of these three species collected from patients admitted to an Italian university hospital from2007 to 2014. Molecular identification of cryptic species of the C. parapsilosis complex was performed using polymerase chain reaction amplification of the gene encoding secondary alcohol dehydrogenase, followed by digestion with the restriction enzyme BanI. Minimum inhibitory concentrations were determined using the broth microdilution method according to European Committee for Antimicrobial Susceptibility Testing (EUCAST EDef 7.2) and Clinical Laboratory Standards Institute (CLSI M27-A3) guidelines, and the results were compared with those obtained using the E-test and Sensititre methods. Of the 163 C. parapsilosis complex isolates, 136 (83.4%) were identified as C. parapsilosis, and 27 (16.6%) as C. orthopsilosis. The species-specific incidences were 2.9/10,000 admissions for C. parapsilosis and 0.6/10,000 admissions for C. orthopsilosis. No resistance to echinocandins was detected with any of the methods. The percent essential agreement (EA) between the EUCAST and E-test/Sensititre methods for anidulafungin, caspofungin, and micafungin susceptibility was, respectively, as follows: C. parapsilosis, 95.6/97.8, 98.5/88.2, and 93.4/96.3; C. orthopsilosis, 92.6/92.6, 96.3/77.8, and 63.0/66.7. The EA between the CLSI and E-test/Sensititre methods was, respectively, as follows: C. parapsilosis, 99.3/100, 98.5/89.0, and 96.3/98.5; C. orthopsilosis, 96.3/92.6, 100/81.5, and 92.6/88.9. Onlyminor discrepancies, ranging from 16.9%(C. parapsilosis) to 11.1%(C. orthopsilosis), were observed between the CLSI and E-test/Sensititre methods. In conclusion, this epidemiologic study shows a typical C. parapsilosis complex species distribution, no echinocandin resistance, and it reinf

Published
2016

23. Food labelling. Regulations and Public Health implications

Author

Marcotrigiano, V, Lanzilotti, C, Rondinone, D, De Giglio, O, Caggiano, G, Diella, G, Orsi, G B, Montagna, M T, Napoli, C, and Orsi, Giovanni Battista

Subjects
labelling, Legislation, Food, Truth Disclosure, food safety, legislation, Choice Behavior, Italy, Food Labeling, Humans, European Union, Public Health, Nutritive Value, Food Hypersensitivity
Abstract

Legislators have implemented policies to improve food labelling to protect consumers and to make the presentation of ingredients and nutritional information more transparent. Proper food labelling allows consumers who may suffer from food allergies or intolerances to know exactly what ingredients a product contains, and it also helps them make more informed health and nutrition choices. This paper deals with the most current European and Italian legislation on food labelling, actions taken in non-EU countries to increase health choices, and the expected impact on Public Health.

Published
2018

24. Corrigendum: Environmental distribution of Cryptococcus neoformans and C. gattii around the Mediterranean basin [FEMS, (2016), 16, 4] doi: http://dx.doi.org/10.1093/femsyr/fow045

Author

Cogliati M., D'Amicis R., Zani A., Montagna M. T., Caggiano G., De Giglio O., Balbino S., De Donno A., Serio F., Susever S., Ergin C., Velegraki A., Ellabib M. S., Nardoni S., Macci C., Oliveri S., Trovato L., Dipineto L., Rickerts V., McCormick-Smith I., Akcaglar S., Tore O., Mlinaric-Missoni E., Bertout S., Mallie M., Martins M. L., Venca A. C. F., Vieira M. L., Sampaio A. C., Pereira C., Criseo G., Romeo O., Ranque S., Al-Yasiri M. H. Y., Kaya M., Cerikcioglu N., Marchese A., Vezzulli L., Ilkit M., Desnos-Ollivier M., Pasquale V., Korem M., Polacheck I., Scopa A., Meyer W., Ferreira-Paim K., Hagen F., Theelen B., Boekhout T., Lockhart S. R., Tintelnot K., Tortorano A. M., Dromer F., Varma A., Kwon-Chung K. J., Inacio J., Alonso B., Colom M. F., Cogliati, M., D'Amicis, R., Zani, A., Montagna, M. T., Caggiano, G., De Giglio, O., Balbino, S., De Donno, A., Serio, F., Susever, S., Ergin, C., Velegraki, A., Ellabib, M. S., Nardoni, S., Macci, C., Oliveri, S., Trovato, L., Dipineto, L., Rickerts, V., McCormick-Smith, I., Akcaglar, S., Tore, O., Mlinaric-Missoni, E., Bertout, S., Mallie, M., Martins, M. L., Venca, A. C. F., Vieira, M. L., Sampaio, A. C., Pereira, C., Criseo, G., Romeo, O., Ranque, S., Al-Yasiri, M. H. Y., Kaya, M., Cerikcioglu, N., Marchese, A., Vezzulli, L., Ilkit, M., Desnos-Ollivier, M., Pasquale, V., Korem, M., Polacheck, I., Scopa, A., Meyer, W., Ferreira-Paim, K., Hagen, F., Theelen, B., Boekhout, T., Lockhart, S. R., Tintelnot, K., Tortorano, A. M., Dromer, F., Varma, A., Kwon-Chung, K. J., Inacio, J., Alonso, B., and Colom, M. F.

Abstract

The original article published in FEMS Yeast Research Vol. 16. Iss. 4; doi: http://dx.doi.org/10.1093/femsyr/fow045 This paper has been updated to correct a spelling error concerning an author name. It previously showed as Giuseppe Griseo, however the correct spelling is Giuseppe Criseo.

Published
2016

25. Control measures for legionellosis in italian hospitals: a national survey

Author

Montagna, M. T., De Giglio, O., Rutigliano, S., Pacifico, C., Napoli, C., Agodi, A., Auxilia, F., Baldovin, T., Bisetto, F., Brusaferro, S., Busetti, M., Calagreti, G, Casini, B., Cristina, M. L., Di Luzio, R., Fiorio, M., Formoso, M., Liguori, G., Martini, E., Molino, A., Mondello, P., Mura, . I., Novati, R., Orsi, G. B., Patroni, A., Poli, A., Privitera, G., Ripabelli, G., Rocchetti, A., Rose, F., Sarti, M., Savini, S., Silvestri, A., Sodano, L., Tardivo, S., Teti, V., Torregrossa, M. V., Torri, E., Veronesi, L., Zarrilli, R., Goglio, A., Moro, M., and Pasquarella, C.

Published
2017

26. Legionella indoor air contamination in healthcare environments

Author

Montagna, M. T., De Giglio, O., Cristina, M. L., Albertini, R., Pasquarella, C., Agodi, A., Coniglio, M. A., Baldovin, T., Spagnolo, A. M., D'Errico, M. M., Delia, S. A., Lagana, P., Giuliano, A., Liguori, G., Guida, M., Torre, I., Pennino, F., Diella, G., Divenuto, F., Rutigliano, S., Caggiano, G., Moro, M., Mura, I., Deriu, M. G., Napoli, C., Romano Spica, V., Valeriani, F., Rossini, A., Privitera, G., Casini, B., Tardivo, S., Sembeni, S., Torregrossa, M. V., Cannova, L., and Villafrate, M. R.

Subjects
Indoor air, Legionella, Water, Bioaerosol
Published
2017

27. A multicenter study on Legionella air contamination in healthcare facilities: comparison of different sampling methods

Author

De Giglio, O., Montagna, M. T., Diella, G., Divenuto, F., Pacifico, C., Rutigliano, S., Cristina, M. L., Napoli, C., Agodi, A., Baldovin, T., Casini, B., Coniglio, M. A., D’Errico, M. M., Delia, A. S., Deriu, M. G., Guida, M., Laganà, P., Liguori, G., Moro, M., Mura, I., Pennino, F., Privitera, G., Romano Spica, V., Sembeni, S., Spagnolo, A. M., Tardivo, S., Torre, I., Valeriani, F., Albertini, R., Pasquarella, C., GISIO-SItI, Aia, and and SIMPIOS Working, Groups.

Published
2017

28. Qualità dell’aria in sala operatoria: uso di modelli parametrici in BIM (Building Information Modeling) al fine di individuare criticità e interferenza tra aspetti edilizi e impiantistici

Author

D’Amico, A., Montagna, M. T., Caggiano, G., De Giglio, O., Lopuzzo, M., Mascipinto, S., Napoli, C., Pacifico, C., Rutigliano, S., Albertini, R., Pasquarella, C., D’Alessandro, D., and Currà, E.

Subjects
Operation theaters, Microbiological pollution, Building Information Modeling, Hospital Design, Operation theaters, Building Information Modeling, Hospital Design, Microbiological pollution
Published
2017

29. The Clean Care Contest: promoting hand hygiene among healthcare and medical students.

Author

Piscitelli, A., Agodi, A., Agozzino, E., Arrigoni, C., Barchitta, M., Brusaferro, S., Castaldi, S., Castiglia, P., Cozzi, L., D'Errico, M. M., De Caro, F., De Giglio, O., Iannazzo, S., Laganà, P., Laurenti, P., Mascipinto, S., Montagna, M. T., Mura, I., Pasquarella, I., and Veronesi, L.

Subjects
HAND hygiene education, MEDICAL care, MEDICAL students, PUBLIC health
Abstract

Introduction. Despite continuing efforts, compliance rates and knowledge of best practices in hand hygiene remain disappointing. Recognizing that conventional educational tools seem out of touch with young people and that the med and messages contents need refreshing, the Italian Study Group of Hospital Hygiene of the Italian Society of Hygiene, Preventive Medicine and Public Health devised a novel approach to promote the creation of innovative educational tools for improving knowledge of, and compliance with, hand hygiene rules among healthcare and medical students. Methods. A contest in creating educational material on hand hygiene practices involved university students of nursing and medicine, and of other healthcare degrees. Students from the universities of the GISIO network were invited to create educational material (e.g., videos, posters, presentations, leaflets, and screensavers) to be presented by May 5th 2019 during the World Hand Hygiene Day / Save Lives: Clean Your Hands Global Annual Initiative of the World Health Organization). A local and a national winners were awarded. Results. Three different local and national contests were performed during 2016, 2017 and 2018. During the three-year period, more than 270 educational tools have been developed: 130 (48%) were judged useful for hand hygiene promotion campaigns. The most frequent projects participating in the contests were videos (39%), posters (29%), leaflets (14%), and others (18%) submitted by more than 1,500 students of nursing (40%), medicine (31%), dentistry (7%), and of other healthcare courses in 14 universities. Products were evaluated by a local committee and, subsequently, local winners represented their University in a national contest. Conclusions. The contest provided a framework for the creation of innovative and potentially effective educational tools via an engaging approach that leveraged student creativity. Given the need to improve compliance rates, this study suggests that new ways can be advantageously explored to teach hand hygiene procedures and increase awareness of the importance of their consistent use among healthcare and medical students. [ABSTRACT FROM AUTHOR]

Published
2020
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30. Environmental distribution of Cryptococcus neoformans and Cryptococcus gattii around the Mediterranean basin

Author

Cogliati, M, D'Amicis, R, Zani, A, Montagna, Mt, Caggiano, G, De Giglio, O, Balbino, S, De Donno, A, Serio, F, Susever, S, Ergin, C, Velegraki, A, Ellabib, Ms, Nardoni, S, Macci, C, Oliveri, Salvatore, Trovato, L, Dipineto, L, Rickerts, V, McCormick Smith, I, Akcaglar, S, Tore, O, Mlinaric Missoni, E, Bertout, S, Mallié, M, Martins, Md, Vencà, Ac, Vieira, Ml, Sampaio, Ac, Pereira, C, Griseo, G, Romeo, O, Ranque, S, Al Yasiri MH, Kaya, M, Cerikcioglu, N, Marchese, A, Vezzulli, L, Ilkit, M, Desnos Ollivier, M, Pasquale, V, Korem, M, Polacheck, I, Scopa, A, Meyer, W, Ferreira Paim, K, Hagen, F, Theelen, B, Boekhout, T, Lockhart, Sr, Tintelnot, K, Tortorano, Am, Dromer, F, Varma, A, Kwon Chung KJ, Inácio, J, Alonso, B, and Colom, M. F.

Published
2016

31. Environmental distribution of Cryptococcus neoformans and C. gattii around the Mediterranean basin

Author

Cogliati M, D'Amicis R, Zani A, Montagna MT, Caggiano G, De Giglio O, Balbino S, De Donno A, Serio F, Susever S, Ergin C, Velegraki A, Ellabib MS, Nardoni S, Macci C, Oliveri S, Trovato L, Dipineto L, Rickerts V, McCormick-Smith I, Akcaglar S, Tore O, Mlinaric-Missoni E, Bertout S, Mallié M, Martins MD, Vencà AC, Vieira ML, Sampaio AC, Pereira C, Criseo G, Romeo O, Ranque S, Al-Yasiri MH, Kaya M, Cerikcioglu N, Marchese A, Vezzulli L, Ilkit M, Desnos-Ollivier M, Pasquale V, Korem M, and Polacheck I

Subjects
Cryptococcus gattii/classification/genetics/*isolation & purification, Cryptococcus neoformans/classification/genetics/*isolation & purification, Environmental Microbiology, Genotype, Mediterranean Region, Molecular Typing, Mycological Typing Techniques, Trees/*microbiology
Abstract

In order to elucidate the distribution of Cryptococcus neoformans and C. gattii in the Mediterranean basin, an extensive environmental survey was carried out during 2012-2015. A total of 302 sites located in 12 countries were sampled, 6436 samples from 3765 trees were collected and 5% of trees were found to be colonized by cryptococcal yeasts. Cryptococcus neoformans was isolated from 177 trees and C. gattii from 13. Cryptococcus neoformans colonized 27% of Ceratonia, 10% of Olea, Platanus and Prunus trees and a lower percentage of other tree genera. The 13 C. gattii isolates were collected from five Eucalyptus, four Ceratonia, two Pinus and two Olea trees. Cryptococcus neoformans was distributed all around the Mediterranean basin, whereas C. gattii was isolated in Greece, Southern Italy and Spain, in agreement with previous findings from both clinical and environmental sources. Among C. neoformans isolates, VNI was the prevalent molecular type but VNII, VNIV and VNIII hybrid strains were also isolated. With the exception of a single VGIV isolate, all C. gattii isolates were VGI. The results confirmed the presence of both Cryptococcus species in the Mediterranean environment, and showed that both carob and olive trees represent an important niche for these yeasts.

Published
2016

33. Observational study on hospital building heritage and microbiological air quality in the orthopedic operating theater: the IM.PA.C.T. Project.

Author

D'Amico, A., Montagna, M. T., Caggiano, G., De Giglio, O., Rutigliano, S., Lopuzzo, M., Mascipinto, S., Napoli, C., Currà, E., and D'Alessandro, D.

Subjects
HOSPITAL buildings, MICROBIOLOGY, AIR quality, OPERATING rooms, TURBULENT flow
Abstract

Background. The study investigated 35 orthopedic OTs [17 with mixed flow (M-OTs), 18 with turbulent flow (T-OTs)]. Methods. The OTs were divided into two categories based on recurring architectural and construction solutions, collected by a survey form: type-A (recently built or renovated rooms), and type-B (other OTs). Assessment of microbial air contamination (colony forming units (cfu)/m3 obtained by active sampling via Surface Air System) was then performed. Results. In 97% of the OTs, a Total Viable Count (TVC) was within the limits recommended by ISPESL 2009; all A-type OTs, and 94% of B-type passed. The TVC of type-A OTs [median 15 cfu/m3, range 3-158] was lower than that of type-B OTs [median 28 cfu/m3, range 6-206], although the difference was not significant. The number of people in type-A [mean 8.6, range 6-11] was lower than in type-B [mean 9.6, range 7-13] OTs, and when adjusted to the volume of the OT (person/m3), showed a significant correlation with TVC (p = 0.383, p <0.05). Conclusions. In conclusion, the structural factors examined do not appear to significantly affect the microbiological air quality at the specific sampling point. However, further investigations are required to identify the factors that have the greatest effect on TVC. [ABSTRACT FROM AUTHOR]

Published
2019
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34. Legionellosis in health care facilities: state of the art in control and prevention in Italy.

Author

Napoli, C., De Giglio, O., Bertamino, E., and Montagna, M. T.

Subjects
LEGIONNAIRES' disease, HEALTH facilities, HEALTH risk assessment, MEDICAL care
Abstract

Background. Nosocomial water systems may be contaminated by Legionella spp; therefore, health care facilities represent a potential health risk for patients and health care staff. Active, well-planned clinical and environmental surveillance in hospitals is the most important instrument of prevention. Aim and Methods. The aim of the present article was to outline the state of the art in legionellosis control and prevention among Italian health care facilities by reporting some experiences in the field. Results. Our results showed that Legionella spp. are largely reported as both hospital water system contaminants and etiological agents in water-related health care-associated infections (HCAI) in Italy. Among the numerous sources of HCAI, water is the most investigated, although it has been demonstrated that air sampling may provide additional information for risk assessment. Conclusions. More appropriate risk assessment is needed, especially in large facilities. In addition, more sensitive diagnostic tests should be used and dedicated training courses should be implemented in health care facilities. [ABSTRACT FROM AUTHOR]

Published
2019
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35. The rapid spread of SARS-COV-2 Omicron variant in Italy reflected early through wastewater surveillance

Author

La Rosa, G., Iaconelli, M., Veneri, C., Mancini, P., Bonanno Ferraro, G., Brandtner, D., Lucentini, L., Bonadonna, L., Rossi, M., Grigioni, M., Suffredini, E., Giuseppe, Bucciarelli, Paolo, Torlontano, Giuseppe, Michele La Bianca, Rosa Anna Cifarelli, Achille, Palma, Giovanna La Vecchia, Giuseppe, Lauria, Rosanna, Brienza, Patrizia, Montenegro, Angelo, D'Argenzio, Luigi, Cossentino, Renato, Olivares, Antonio, Pizzolante, Giovanna, Fusco, Alessandra, Tosco, Amalia, Porta, Francesca, Pennino, Triassi, Maria, Paola, Angelini, Laura De Lellis, Daniele, Nasci, Giovanni, Alborali, Nicoletta, Formenti, Flavia, Guarneri, Nadia, Fontani, Giulia, Nani, Franca, Palumbo, Gianluca, Borlone, Marco, Guercio, Lisa, Gentili, Marika, Mariuz, Gabriella, Trani, Anna, Pariani, Carla, Ancona, Doriana Antonella Giorgi, Irene, Ferrante, Monica, Monfrinotti, Silvia, Riosa, Valeria, Capparuccini, Maria Teresa Scicluna, Antonella, Mariaconcetta, Arizzi, Giancarlo, Cecchini, Claudio, Ottaviano, Elena, Nicosia, Elena, Grasselli, Giorgia, Allaria, Alberto, Izzotti, Stefano, Rosatto, Emanuela, Ammoni, Danilo, Cereda, Marina Nadia Losio, Barbara, Bertasi, Andrea, Aliscioni, Desdemona, Oliva, Sara, Castiglioni, Silvia, Schiarea, Ettore, Zuccato, Manuela, Antonelli, Arianna, Azzellino, Francesca, Malpei, Andrea, Turolla, Sandro, Binda, Pellegrinelli, Laura, Valeria, Primache, Clementina, Cocuzza, Andrea, Franzetti, Giorgio, Bertanza, Maria Luisa Callegari, Luigi, Bolognini, Fabio, Filippetti, Marta, Paniccia, Francesca, Ciuti, Sara, Briscolini, Silvia, Magi, Michele, Colitti, Carmen, Montanaro, Giuseppe, Aprea, Maria Grazia Cerroni, Bartolomeo, Griglio, Renza, Berruti, Mauro, Cravero, Angela, Costa, Manila, Bianchi, Lucia, Decastelli, Angelo, Romano, Fabio, Zuccon, Elisabetta, Carraro, Cristina, Pignata, Silvia, Bonetta, Giuseppe Di Vittorio, Onofrio, Mongelli, Osvalda De Giglio, Francesca, Apollonio, Francesco, Triggiano, Maria Teresa Montagna, Nicola, Ungaro, Mario, Palermo, Carmelo Massimo Maida, Walter, Mazzucco, Simona De Grazia, Giovanni, Giammanco, Giuseppa, Purpari, Margherita, Ferrante, Antonella, Agodi, Martina, Barchitta, Piergiuseppe, Cala’, Carducci, Annalaura, Verani, Marco, Federigi, Ileana, Giulia, Lauretani, Sara, Muzio, Matteo, Ramazzotti, Alberto, Antonelli, Enrica, Ricci, Giovanni, Santoro, Ermanno, Federici, Maya, Petricciuolo, Sofia, Barigelli, Mauro, Ruffier, Francesca, Borney, Eric, Grange, Florida, Damasco, Francesca, Russo, Gisella, Pitter, Vanessa, Groppi, Franco, Rigoli, Marco, Zampini, Tatjana, Baldovin, Irene, Amoruso, Elena, Mengon, Maria, Cadonna, Mattia, Postinghel, Francesco, Pizzo, Alessandra, Schiavuzzi, Francesca, Cutrupi, Paola, Foladori, Serena, Manara, Lorella, Zago, Alberta, Stenico, Anna-Maria, Prast., La Rosa, G, Iaconelli, M, Veneri, C, Mancini, P, Bonanno Ferraro, G, Brandtner, D, Lucentini, L, Bonadonna, L, Rossi, M, Grigioni, M, Suffredini, E, Bucciarelli, G, Torlontano, P, Aprea, G, La Bianca, M, Cifarelli, R, Palma, A, La Vecchia, G, Lauria, G, Brienza, R, Montenegro, P, D'Argenzio, A, Cossentino, L, Olivares, R, Pizzolante, A, Fusco, G, Tosco, A, Porta, A, Pennino, F, Maria, T, Angelini, P, De Lellis, L, Nasci, D, Alborali, G, Formenti, N, Guarneri, F, Fontani, N, Nani, G, Palumbo, F, Borlone, G, Guercio, M, Gentili, L, Mariuz, M, Trani, G, Pariani, A, Ancona, C, Giorgi, D, Ferrante, I, Monfrinotti, M, Riosa, S, Capparuccini, V, Scicluna, M, Cersini, A, Arizzi, M, Cecchini, G, Ottaviano, C, Nicosia, E, Grasselli, E, Allaria, G, Izzotti, A, Rosatto, S, Ammoni, E, Cereda, D, Losio, M, Bertasi, B, Aliscioni, A, Oliva, D, Castiglioni, S, Schiarea, S, Zuccato, E, Antonelli, M, Azzellino, A, Malpei, F, Turolla, A, Binda, S, Laura, P, Primache, V, Cocuzza, C, Franzetti, A, Bertanza, G, Callegari, M, Bolognini, L, Filippetti, F, Paniccia', M, Ciuti, F, Briscolini, S, Magi, S, Colitti, M, Montanaro, C, Cerroni, M, Griglio, B, Berruti, R, Cravero, M, Costa, A, Bianchi, M, Decastelli, L, Romano, A, Zuccon, F, Carraro, E, Pignata, C, Bonetta, S, Di Vittorio, G, Mongelli, O, De Giglio, O, Apollonio, F, Triggiano, F, Montagna, M, Ungaro, N, Palermo, M, Maida, C, Mazzucco, W, De Grazia, S, Giammanco, G, Purpari, G, Ferrante, M, Agodi, A, Barchitta, M, Cala', P, Carducci, A, Verani, M, Federigi, I, Lauretani, G, Muzio, S, Ramazzotti, M, Antonelli, A, Ricci, E, Santoro, G, Federici, E, Petricciuolo, M, Barigelli, S, Ruffier, M, Borney, F, Grange, E, Damasco, F, Russo, F, Pitter, G, Groppi, V, Rigoli, F, Zampini, M, Baldovin, T, Amoruso, I, Mengon, E, Cadonna, M, Postinghel, M, Pizzo, F, Schiavuzzi, A, Cutrupi, F, Foladori, P, Manara, S, Zago, L, Stenico, A, Prast, A, La Rosa G., Iaconelli M., Veneri C., Mancini P., Bonanno Ferraro G., Brandtner D., Lucentini L., Bonadonna L., Rossi M., Grigioni M., Bucciarelli G., Torlontano P., Aprea G., La Bianca M., Cifarelli R.A., Palma A., La Vecchia G., Lauria G., Brienza R., Montenegro P., D'Argenzio A., Cossentino L., Olivares R., Pizzolante A., Fusco G., Tosco A., Porta A., Pennino F., Maria T., Angelini P., De Lellis L., Nasci D., Alborali G., Formenti N., Guarneri F., Fontani N., Nani G., Palumbo F., Borlone G., Guercio M., Gentili L., Mariuz M., Trani G., Pariani A., Ancona C., Giorgi D.A., Ferrante I., Monfrinotti M., Riosa S., Capparuccini V., Scicluna M.T., Cersini A., Arizzi M., Cecchini G., Ottaviano C., Nicosia E., Grasselli E., Allaria G., Izzotti A., Rosatto S., Ammoni E., Cereda D., Losio M.N., Bertasi B., Aliscioni A., Oliva D., Castiglioni S., Schiarea S., Zuccato E., Antonelli M., Azzellino A., Malpei F., Turolla A., Binda S., Laura P., Primache V., Cocuzza C., Franzetti A., Bertanza G., Callegari M.L., Bolognini L., Filippetti F., Paniccia' M., Ciuti F., Briscolini S., Magi S., Colitti M., Montanaro C., Cerroni M.G., Griglio B., Berruti R., Cravero M., Costa A., Bianchi M., Decastelli L., Romano A., Zuccon F., Carraro E., Pignata C., Bonetta S., Di Vittorio G., Mongelli O., De Giglio O., Apollonio F., Triggiano F., Montagna M.T., Ungaro N., Palermo M., Maida C.M., Mazzucco W., De Grazia S., Giammanco G., Purpari G., Ferrante M., Agodi A., Barchitta M., Cala' P., Carducci A., Verani M., Federigi I., Lauretani G., Muzio S., Ramazzotti M., Antonelli A., Ricci E., Santoro G., Federici E., Petricciuolo M., Barigelli S., Ruffier M., Borney F., Grange E., Damasco F., Russo F., Pitter G., Groppi V., Rigoli F., Zampini M., Baldovin T., Amoruso I., Mengon E., Cadonna M., Postinghel M., Pizzo F., Schiavuzzi A., Cutrupi F., Foladori P., Manara S., Zago L., Stenico A., Prast A.-M., Suffredini E., and Triassi, M

Subjects
Omicron, RT-qPCR, SARS-CoV-2, Sewage, Variant, Wastewater-based epidemiology, Wastewater-Based Epidemiological Monitoring, Environmental Engineering, COVID-19, Wastewater, Pollution, Humans, RNA, Viral, Waste Water, SARS-Cov2, Environmental Chemistry, RNA, Viral, wastewater based epidemiology, Waste Management and Disposal, Human, Omicron, RT-qPCR, SARS-CoV-2, Sewage, Variant, Wastewater-based epidemiology
Abstract

The SARS-CoV-2 Omicron variant emerged in South Africa in November 2021, and has later been identified worldwide, raising serious concerns. A real-time RT-PCR assay was designed for the rapid screening of the Omicron variant, targeting characteristic mutations of the spike gene. The assay was used to test 737 sewage samples collected throughout Italy (19/21 Regions) between 11 November and 25 December 2021, with the aim of assessing the spread of the Omicron variant in the country. Positive samples were also tested with a real-time RT-PCR developed by the European Commission, Joint Research Centre (JRC), and through nested RT-PCR followed by Sanger sequencing. Overall, 115 samples tested positive for Omicron SARS-CoV-2 variant. The first occurrence was detected on 7 December, in Veneto, North Italy. Later on, the variant spread extremely fast in three weeks, with prevalence of positive wastewater samples rising from 1.0% (1/104 samples) in the week 5-11 December, to 17.5% (25/143 samples) in the week 12-18, to 65.9% (89/135 samples) in the week 19-25, in line with the increase in cases of infection with the Omicron variant observed during December in Italy. Similarly, the number of Regions/Autonomous Provinces in which the variant was detected increased from one in the first week, to 11 in the second, and to 17 in the last one. The presence of the Omicron variant was confirmed by the JRC real-time RT-PCR in 79.1% (91/115) of the positive samples, and by Sanger sequencing in 66% (64/97) of PCR amplicons. In conclusion, we designed an RT-qPCR assay capable to detect the Omicron variant, which can be successfully used for the purpose of wastewater-based epidemiology. We also described the history of the introduction and diffusion of the Omicron variant in the Italian population and territory, confirming the effectiveness of sewage monitoring as a powerful surveillance tool.

Published
2022

36. Assessing Undergraduates' Perception of Risks Related to Body Art in Italy: The SUPeRBA Multicenter Cross-Sectional Study

Author

Carmela Protano, Federica Valeriani, Daniela Marotta, Annalisa Bargellini, Aida Bianco, Giuseppina Caggiano, Maria Eugenia Colucci, Maria Anna Coniglio, Laura Dallolio, Osvalda De Giglio, Gabriella Di Giuseppe, Pasqualina Laganà, Francesca Licata, Giorgio Liguori, Isabella Marchesi, Sofia Marini, Maria Teresa Montagna, Christian Napoli, Giovanni Battista Orsi, Cesira Pasquarella, Concetta Paola Pelullo, Vincenzo Romano Spica, Rossella Sacchetti, Stefano Tardivo, Licia Veronesi, Matteo Vitali, Francesca Gallè, Protano C., Valeriani F., Marotta D., Bargellini A., Bianco A., Caggiano G., Colucci M.E., Coniglio M.A., Dallolio L., De Giglio O., Di Giuseppe G., Lagana P., Licata F., Liguori G., Marchesi I., Marini S., Montagna M.T., Napoli C., Orsi G.B., Pasquarella C., Pelullo C.P., Spica V.R., Sacchetti R., Tardivo S., Veronesi L., Vitali M., Galle F., Protano, C., Valeriani, F., Marotta, D., Bargellini, A., Bianco, A., Caggiano, G., Colucci, M. E., Coniglio, M. A., Dallolio, L., De Giglio, O., Di Giuseppe, G., Lagana, P., Licata, F., Liguori, G., Marchesi, I., Marini, S., Montagna, M. T., Napoli, C., Orsi, G. B., Pasquarella, C., Pelullo, C. P., Spica, V. R., Sacchetti, R., Tardivo, S., Veronesi, L., Vitali, M., and Galle, F.

Subjects
Adult, medicine.medical_specialty, knowledge, Adolescent, complications, Cross-sectional study, Health, Toxicology and Mutagenesis, media_common.quotation_subject, body art, piercing, tattooing, undergraduates, health, awareness, Specific knowledge, Article, Awareness, Body art, Complications, Health, Knowledge, Piercing, Tattooing, Undergraduates, Cross-Sectional Studies, Humans, Italy, Perception, Students, Young Adult, Body Piercing, Medicine, Health risk, media_common, Undergraduate, Cross-Sectional Studie, Health consequences, business.industry, Public Health, Environmental and Occupational Health, Attendance, Awarene, Mean age, Large sample, Family medicine, Student, business, Complication, Human
Abstract

Tattooing and piercing may lead to health complications. The present multicenter cross-sectional study aimed to assess awareness and knowledge of health risks related to body art and to identify their possible determinants among a large sample of undergraduates in Italy. A web-based questionnaire collecting information on socio-demographic characteristics, awareness, knowledge, and some potential predictors was administered to undergraduates attending twelve Italian universities. The level of knowledge was expressed as the number of correct answers (0–11 for tattooing, 0–14 for piercing). A total of 2985 participants (mean age 23.15 ± 3.99, 73.9% F) participated in the study. Although 95.4% of the respondents were aware of possible health consequences of body art, a low level of specific knowledge was registered for both tattooing (mean number of correct answers 5.38 ± 2.39) and piercing (5.93 ± 3.12) consequences. Lower knowledge was associated with the attendance of non-life science course and with lower duration of academic education for both tattoo and piercing. Lower knowledge of tattooing risks was related with commuter status, while lower knowledge of piercing risks was associated with lower father’s education. These findings highlight the need to enhance information campaigns targeted to youths to increase their awareness of possible health risk of body art.

Published
2021

37. Impact of the environment on the health: From theory to practice

Author

A.L. Carducci, A. Agodi, C. Ancona, P. Angelini, F. Bagordo, F. Barbone, L. Birbaum, V. Carreri, A. Casuccio, A. Conti, M. Conversano, A. De Donno, O. De Giglio, F. Desiante, A. Di Pietro, E. Dogliotti, F. Donato, G.M. Fara, M. Fiore, F. Forastiere, G. Giammanco, A. Izzotti, M.T. Montagna, G. Oliveri Conti, M.G. Petronio, S. Sciacca, C. Signorelli, E. Testai, M. Verani, M. Vinceti, F. Vitale, M. Ferrante, G. Adani, L. Berghella, C. Calia, R. Calzolari, A. Canale, D. Castiglione, C. Copat, A. Cristaldi, G. Cuffari, G. Coronel Vargas, E. De Vita, F. De Nard, I. Federigi, T. Filippini, A. Grasso, N. Leonardi, M. Letzgus, G. Lo Bianco, W. Mazzucco, I. Nicolosi, P. Orlandi, G. Paladino, S. Pizzo, C. Pousis, M. Raffo, S. Rivolta, F. Scarpitta, G. Trani, F. Triggiano, A. Tumbarello, V. Vecchio, P. Zuccarello, M. Vassallo, Al, Carducci, Agodi, A, Ancona, C, Angelini, P, Bagordo, F, Barbone, F, Birbaum, L, V, Carreri, Casuccio, A, Conti, A, Conversano, M, De Donno, A, De Giglio, O, Desiante, F, Di Pietro, A, Dogliotti, E, Donato, F, Gm, Fara, Fiore, M, Forastiere, F, Giammanco, G, Izzotti, A, Montagna, M T, Oliveri Conti, G, Mg, Petronio, Sciacca, S, Signorelli, C, Testai, E, Verani, M, Vinceti, M, Vitale, F, Ferrante, M, Adani, G, Berghella, L, Calia, C, Calzolari, R, Canale, A, Castiglione, D, Copat, C, Cristaldi, A, Cuffari, G, Coronel Vargas, G, De Vita, E, De Nard, F, Federigi, I, Filippini, T, Grasso, A, Leonardi, N, Letzgus, M, Lo Bianco, G, Mazzucco, W, Nicolosi, I, Orlandi, P, Paladino, G, Pizzo, S, Pousis, C, Raffo, M, Rivolta, S, Scarpitta, F, Trani, G, Triggiano, F, Tumbarello, A, Vecchio, V, Zuccarello, P, M, Vassallo, Carducci, A. L., Agodi, A., Ancona, C., Angelini, P., Bagordo, F., Barbone, F., Birbaum, L., Carreri, V., Casuccio, A., Conti, A., Conversano, M., De Donno, A., De Giglio, O., Desiante, F., Di Pietro, A., Dogliotti, E., Donato, F., Fara, G. M., Fiore, M., Forastiere, F., Giammanco, G., Izzotti, A., Montagna, M. T., Oliveri Conti, G., Petronio, M. G., Sciacca, S., Signorelli, C., Testai, E., Verani, M., Vinceti, M., Vitale, F., Ferrante, M., Adani, G., Berghella, L., Calia, C., Calzolari, R., Canale, A., Castiglione, D., Copat, C., Cristaldi, A., Cuffari, G., Coronel Vargas, G., De Vita, E., De Nard, F., Federigi, I., Filippini, T., Grasso, A., Leonardi, N., Letzgus, M., Lo Bianco, G., Mazzucco, W., Nicolosi, I., Orlandi, P., Paladino, G., Pizzo, S., Pousis, C., Raffo, M., Rivolta, S., Scarpitta, F., Trani, G., Triggiano, F., Tumbarello, A., Vecchio, V., Zuccarello, P., and Vassallo, M.

Subjects
medicine.medical_specialty, Epidemiological and toxicological study, education, 010501 environmental sciences, Global Health, Settore MED/42 - Igiene Generale E Applicata, 01 natural sciences, Biochemistry, Risk communication, 03 medical and health sciences, 0302 clinical medicine, Environmental Impact Assessment, Health Impact Assessment, epidemiological and toxicological study, exposure assessment, risk communication, urban health, Political science, medicine, Global health, Humans, Environmental impact assessment, Exposure assessment, Health impact assessment, Urban health, 030212 general & internal medicine, Sicily, 0105 earth and related environmental sciences, General Environmental Science, Preventive healthcare, epidemiological and toxicological study exposure assessment, urban health risk communication, Medical education, Public health, Charter, Environmental Health, Public Health, One Health, Health Impact Assessment , Environmental Impact Assessment, epidemiological and toxicological study exposure assessment, urban health risk communication, Risk assessment, Working group
Abstract

The Erice 56 Charter titled "Impact of the environment on the health: from theory to practice" was unanimously approved at the end of the 56th course of the "International School of Epidemiology and Preventive Medicine G. D'Alessandro" held from 3th to 7th November 2019 in Erice - Sicily (Italy) and promoted by the Study Group of "Environment and Health" of the Italian Society of Hygiene, Preventive Medicine and Public Health. The course, that included lectures, open discussions and guided working groups, was aimed to provide a general training on epidemiological and toxicological aspects of the environmental health impact, to be used by public health professionals for risk assessment, without forgetting the risk communications. At the end of the course 12 key points were agreed among teachers and students: they underlined the need of specific training and research, in the perspective of "One Health" and "Global Health", also facing emerging scientific and methodological issues and focusing on communication towards stakeholders. This Discussion highlight the need to improve knowledge of Health and Environment topic in all sectors of health and environmental prevention and management.

Published
2021

38. What about your body ornament? Experiences of tattoo and piercing among italian youths

Author

Federica Valeriani, Osvalda De Giglio, Giovanni Battista Orsi, Stefano Tardivo, Licia Veronesi, Matteo Vitali, Isabella Marchesi, Concetta Paola Pelullo, Giusy Diella, Annalisa Bargellini, Giorgio Liguori, Pasqualina Laganà, Carmela Protano, Luca Ricciardi, Maria Anna Coniglio, Aida Bianco, Vincenzo Romano Spica, Maria Eugenia Colucci, Francesca Licata, Christian Napoli, Gabriella Di Giuseppe, Laura Dallolio, Cesira Pasquarella, Daniela Marotta, Maria Teresa Montagna, Andrea De Giorgi, Francesca Gallè, Sofia Marini, Rossella Sacchetti, Galle, F., Valeriani, F., Marotta, D., De Giorgi, A., Bargellini, A., Bianco, A., Colucci, M. E., Coniglio, M. A., Dallolio, L., De Giglio, O., Di Giuseppe, G., Diella, G., Lagana, P., Licata, F., Liguori, G., Marchesi, I., Marini, S., Montagna, M. T., Napoli, C., Orsi, G. B., Pasquarella, C., Pelullo, C. P., Ricciardi, L., Spica, V. R., Sacchetti, R., Tardivo, S., Veronesi, L., Vitali, M., Protano, C., Galle F., Valeriani F., Marotta D., De Giorgi A., Bargellini A., Bianco A., Colucci M.E., Coniglio M.A., Dallolio L., De Giglio O., Di Giuseppe G., Diella G., Lagana P., Licata F., Liguori G., Marchesi I., Marini S., Montagna M.T., Napoli C., Orsi G.B., Pasquarella C., Pelullo C.P., Ricciardi L., Spica V.R., Sacchetti R., Tardivo S., Veronesi L., Vitali M., and Protano C.

Subjects
Male, medicine.medical_specialty, Universities, Adolescent, Health, Toxicology and Mutagenesis, media_common.quotation_subject, Adverse effect, Article, Body piercing, Adverse effects, Prevention and control, Tattooing, Young adults, Female, Humans, Hygiene, Students, Surveys and Questionnaires, Body Piercing, medicine, Surveys and Questionnaire, media_common, body piercing, tattooing, young adults, adverse effects, prevention and control, interests, Public health, Public Health, Environmental and Occupational Health, Universitie, Large sample, Young adult, Sterilization (medicine), Multicenter study, Family medicine, Medicine, Educational interventions, Student, Psychology, interests.hobby, Human
Abstract

Background: tattooing and piercing are increasingly common, especially among youths. However, several health complications may be associated with these practices if basic hygiene rules are not respected. This multicenter study was aimed at exploring tattoo and piercing experiences reported by a large sample of Italian undergraduate students through a public health perspective. Methods: tattooed and/or pierced students attending 12 Italian universities were asked to complete a web-based questionnaire regarding their body art experience. Results: out of 1472 respondents, 833 (56.6%) were tattooed and 1009 (68.5%) were pierced. The greatest proportion of tattooed students (93.9%) got her/his first tattoo in a tattoo studio, while most of the pierced were serviced in a jewelry store (48.0%). The pierced ones were less informed on health issues related to the procedure (56.0% versus 77.8% of tattooed p < 0.001), and tattooists were reportedly more attentive to hygiene rules (instrument sterilization 91.5% versus 79.1% of piercers, p < 0.001; use of disposable gloves 98.2% versus 71% of piercers, p < 0.001). Conclusions: educational interventions for both professionals and communities are needed to improve the awareness and the control of health risks related to body art throughout the Italian territory.

Published
2021

39. Assessment of Dietary Supplement Consumption among Italian University Students: the Multicenter DiSCo Study

Author

Francesca Gallè, Federica Valeriani, Andrea De Giorgi, Silvia Angelillo, Annalisa Bargellini, Aida Bianco, Lavinia Bianco, Giuseppina Caggiano, Maria Eugenia Colucci, Maria Anna Coniglio, Laura Dallolio, Osvalda De Giglio, Gabriella Di Giuseppe, Heba Safwat Mhmoued Abdo Elhadidy, Maria Eufemia Gioffrè, Pasqualina Laganà, Francesca Licata, Isabella Marchesi, Alice Masini, Maria Teresa Montagna, Christian Napoli, Giovanni Battista Orsi, Cesira Pasquarella, Concetta Paola Pelullo, Stefania Paduano, Rossella Sacchetti, Giacomo Scaioli, Roberta Siliquini, Licia Veronesi, Giorgio Liguori, Vincenzo Romano Spica, Matteo Vitali, Carmela Protano, Galle, F., Valeriani, F., De Giorgi, A., Angelillo, S., Bargellini, A., Bianco, A., Bianco, L., Caggiano, G., Colucci, M. E., Coniglio, M. A., Dallolio, L., De Giglio, O., Di Giuseppe, G., Elhadidy, H. S. M. A., Gioffre, M. E., Lagana, P., Licata, F., Marchesi, I., Masini, A., Montagna, M. T., Napoli, C., Orsi, G. B., Pasquarella, C., Pelullo, C. P., Paduano, S., Sacchetti, R., Scaioli, G., Siliquini, R., Veronesi, L., Liguori, G., Spica, V. R., Vitali, M., and Protano, C.

Subjects
Undergraduate, Nutrition and Dietetics, diet supplementation, undergraduate, lifestyle, nutrition, sport, Endocrinology, Diabetes and Metabolism, Diet supplementation, Lifestyle, Nutrition, Sport
Abstract

Objectives: The aim of this study was to analyze the consumption of dietary supplements (DSs) and related sociodemographic characteristics or behaviors in Italian undergraduates. Methods: A questionnaire-based, cross-sectional study was carried out between October 2021 and May 2022 involving undergraduates from 12 public universities. The use of DSs in the previous 6 mo, the aim and reason for use, the purchasing channel, and related adverse effects were explored and compared by sex. A logistic regression was performed to highlight possible associations between sociodemographic, anthropometric, and behavioral characteristics of participants and supplement consumption. Results: The use of DSs was reported by 71.5% of the 2165 respondents. Supplementation was related to sex (odds ratio [OR] 0.690; 95% confidence interval [CI], 0.516-0.924, for men), vegetarian or vegan (OR 4.814; 95% CI, 1.669-13.887) and other particular diet regimens (OR 1.819; 95% CI, 1.282-2.580), sport (OR 1.688; 95% CI, 1.116-2.553), and type of sport (OR 0.629; 95% CI, 0.423-0.936, for team sports). Women were more likely to use DSs following a doctor's prescription and to buy them in a pharmacy (P < 0.001) but reported more adverse effects (P=0.018) than men. The main aim pursued was general health; proteins, amino acids, and non-caffeinated energy supplements were consumed to improve physical performance and caffeinated energy supplements for mental performance. Conclusions: Diet supplementation was common in the sample examined, especially in women, and associated with particular dietary regimens and sports, especially individual sports. Female consumers follow specialists' indications more than males. These results highlight the need for educational interventions about diet supplementation for this category of users.

Published
2022

40. Pro-environmental behaviors: Determinants and obstacles among italian university students

Author

Martina Bortoletto, Federica Violi, Tiziana Schilirò, Fabio Petrelli, Grazia Paladino, Margherita Ferrante, Antonio Azara, Stefania Scuri, Alessio Facciolà, G. Palomba, Marco Vinceti, Marco Dettori, Giuseppina Caggiano, Ileana Federigi, Annalaura Carducci, Marina Tesauro, A Calamusa, Maria Teresa Montagna, Marco Verani, Iolanda Grappasonni, Osvalda De Giglio, Liberata Keti Nicolosi, Chiara Lorini, Alberto Izzotti, Guglielmo Bonaccorsi, Giovanni Libralato, Francesca Di Serio, Angela Di Pietro, Maria Fiore, Pamela Di Giovanni, Antonella De Donno, Carducci, A., Fiore, M., Azara, A., Bonaccorsi, G., Bortoletto, M., Caggiano, G., Calamusa, A., De Donno, A., De Giglio, O., Dettori, M., Di Giovanni, P., Di Pietro, A., Facciola, A., Federigi, I., Grappasonni, I., Izzotti, A., Libralato, G., Lorini, C., Montagna, M. T., Nicolosi, L. K., Paladino, G., Palomba, G., Petrelli, F., Schiliro, T., Scuri, S., Serio, F., Tesauro, M., Verani, M., Vinceti, M., Violi, F., and Ferrante, M.

Subjects
Pro-environmental attitude, Functional health literacy, Health Knowledge, Attitudes, Practice, Pro-environmental behaviors, Universities, Health, Toxicology and Mutagenesis, media_common.quotation_subject, Psychological intervention, lcsh:Medicine, 050109 social psychology, Environmental pollution, Health literacy, 010501 environmental sciences, Functional health, 01 natural sciences, Internal locus of control, Literacy, Article, Risk communication, Environmental health, Surveys and Questionnaires, Surveys and Questionnaire, Humans, 0501 psychology and cognitive sciences, Environmental health risk perception, Pro-environmental attitudes, Students, Health risk perception, 0105 earth and related environmental sciences, media_common, pro-environmental attitudes, pro-environmental behaviors, environmental health risk perception, functional health literacy, risk communication, internal locus of control, Practice, pro-environmental attitudespro-environmental behaviorsenvironmental health risk perceptionfunctional health literacyrisk communicationinternal locus of control, Health Knowledge, lcsh:R, 05 social sciences, Public Health, Environmental and Occupational Health, Attitude, Italy, Risk perception, Locus of control, Pro-environmental behavior, Attitudes, Student, Psychology, Human
Abstract

The awareness of citizens concerning the health risks caused by environmental pollution is growing, but studies on determinants of pro-environmental behaviors have rarely examined health-related aspects. In this study, we investigated these determinants using data from a large survey among Italian university students (15 Universities: 4778 filled questionnaires). Besides the health-related aspects, represented by environmental health risk perception and functional health literacy, we considered social and demographic characteristics (gender, area of residence, sources of information, trust in institutional and non-institutional subjects, and students’ capacity of positive actions, indicated as internal locus of control). The attitudes towards pro-environmental behaviors were positive for more than 70% of students and positively related with health risk perception, internal locus of control, and health literacy. The correspondence between the positive attitudes towards pro-environmental behaviors and the real adoption of such behaviors was approximately 20% for most behaviors, except for the separate collection of waste (60%). Such a discrepancy can be attributable to external obstacles (i.e., lack of time, costs, lack of support). The health-related aspects were linked to the pro-environmental attitudes, but to a lesser extent to pro-environmental behaviors, owing to the complexity of their determinants. However, they should be taken in account in planning education interventions.

Published
2021

41. Microbiological and chemical assessment of wastewater discharged by infiltration trenches in fractured and karstified limestone (Sca.re.s. project 2019–2020)

Author

Silvia Brigida, Cristina De Ceglie, Sapia Murgolo, Pamela Mancini, Antonella De Donno, Giusy Diella, Francesca Apollonio, Marcella Narracci, C. Pousis, Rosa Anna Cavallo, Francesco Triggiano, Maria Immacolata Acquaviva, Giusy Bonanno Ferraro, Claudio Di Iaconi, Maria Clementina Caputo, Giuseppe Mascolo, Tiziana Grassi, Carla Calia, Francesco Bagordo, Maria Teresa Montagna, Carolina Veneri, Osvalda De Giglio, Giuseppina La Rosa, Montagna, M. T., De Giglio, O., Calia, C., Pousis, C., Triggiano, F., Murgolo, S., De Ceglie, C., Bagordo, F., Apollonio, F., Diella, G., Narracci, M., Acquaviva, M. I., Ferraro, G. B., Mancini, P., Veneri, C., Brigida, S., Grassi, T., De Donno, A., Di Iaconi, C., Caputo, M. C., Cavallo, R. A., La Rosa, G., and Mascolo, G.

Subjects
Microbiology (medical), Veterinary medicine, Pepper mild mottle virus, lcsh:Medicine, wastewater, groundwater, bacteria, viru, virus, Article, Percolation trench, groundwater, Immunology and Allergy, bacteria, wastewater, Molecular Biology, contaminants of emerging concern, Bacteria, Contaminants of emerging concern, Groundwater, Virus, Wastewater, geography, geography.geographical_feature_category, General Immunology and Microbiology, biology, lcsh:R, Contamination, Karst, biology.organism_classification, Infiltration (hydrology), Infectious Diseases, Environmental science, Sewage treatment
Abstract

This study investigated the environmental contamination of groundwater as a consequence of the discharge of treated wastewater into the soil. The investigation focused on a wastewater treatment plant located in an area fractured by karst in the Salento peninsula (Apulia, Italy). Water samples were collected at four sites (raw wastewater, treated wastewater, infiltration trench, and monitoring well), monthly from May to December 2019 (with the exception of August), and were tested for 1) panel of bacteria, 2) enteric viruses, and 3) chemical substances. A gradual reduction in the concentration of bacteria, viruses and contaminants of emerging concern was observed across the profile of soil fissured by karst. All monitored bacteria were absent from the monitoring well, except for Pseudomonas aeruginosa. Pepper mild mottle virus and adenovirus were detected at all sampling sites. Personal care products and X-ray contrast media showed the greatest decrease in concentration from infiltration trench to the monitoring well, while the highest residual concentrations in the monitoring well were found for anticonvulsants (78.5%), antimicrobials (41.3%), and antipsychotic drugs (38.6%). Our results show that parameters provided by current law may not always be sufficient to evaluate the sanitary risk relating to the discharge of treated wastewater to the soil.

Published
2020

42. Environment and health: Risk perception and its determinants among Italian university students

Author

Grazia Paladino, Fabio Petrelli, Maria Fiore, Pamela Di Giovanni, Osvalda De Giglio, Angela Di Pietro, Liberata Keti Nicolosi, Marco Verani, Maria Teresa Montagna, G. Palomba, Giuseppina Caggiano, Antonella De Donno, Marina Tesauro, Stefania Scuri, Giovanni Libralato, Chiara Lorini, Alessio Facciolà, Marco Vinceti, Marco Dettori, Iolanda Grappasonni, I. Federigi, Guglielmo Bonaccorsi, Martina Bortoletto, Antonio Azara, Francesca Di Serio, Alberto Izzotti, A Calamusa, Federica Violi, Margherita Ferrante, Tiziana Schilirò, Annalaura Carducci, Carducci, A., Fiore, M., Azara, A., Bonaccorsi, G., Bortoletto, M., Caggiano, G., Calamusa, A., De Donno, A., De Giglio, O., Dettori, M., Di Giovanni, P., Di Pietro, A., Facciola, A., Federigi, I., Grappasonni, I., Izzotti, A., Libralato, G., Lorini, C., Montagna, M. T., Nicolosi, L. K., Paladino, G., Palomba, G., Petrelli, F., Schiliro, T., Scuri, S., Serio, F., Tesauro, M., Verani, M., Vinceti, M., Violi, F., Ferrante, M., Carducci, Annalaura, Fiore, Maria, Azara, Antonio, Bonaccorsi, Guglielmo, Bortoletto, Martina, Caggiano, Giuseppina, Calamusa, Andrea, DE DONNO, Maria Antonella, De Giglio, Osvalda, Dettori, Marco, Di Giovanni, Pamela, Di Pietro, Angela, Facciolà, Alessio, Federigi, Ileana, Grappasonni, Iolanda, Izzotti, Alberto, Libralato, Giovanni, Lorini, Chiara, Teresa Montagna, Maria, Keti Nicolosi, Liberata, Paladino, Grazia, Palomba, Giacomo, Petrelli, Fabio, Schilirò, Tiziana, Scuri, Stefania, Serio, Francesca, Tesauro, Marina, Verani, Marco, Vinceti, Marco, Violi, Federica, and Ferrante, Margherita

Subjects
School, Functional health literacy, Male, Health Knowledge, Attitudes, Practice, 010504 meteorology & atmospheric sciences, Cross-sectional study, Environmental health risk perception, Public health, Risk communication, Students, Adolescent, Adult, Cross-Sectional Studies, Female, Humans, Italy, Risk, Schools, Surveys and Questionnaires, Universities, Young Adult, Environment, Applied psychology, 010501 environmental sciences, 01 natural sciences, Literacy, Global health, Surveys and Questionnaire, Waste Management and Disposal, media_common, Practice, Health Knowledge, Pollution, Environmental health risk perception, Functional health literacy, Public health, Risk communication, Students, Adolescent, Adult, Cross Sectional Studies, Female, Humans, Italy, Male, Risk, Schools, Students, Surveys and Questionnaires, Universities, Young Adult, Environment, Health Knowledge, Attitudes, Practice, Student, Psychology, Human, medicine.medical_specialty, Environmental Engineering, media_common.quotation_subject, Health literacy, medicine, Environmental Chemistry, Social media, 0105 earth and related environmental sciences, Cross-Sectional Studie, Cross Sectional Studies, Universitie, Risk perception, Communication Intervention, Attitudes, Environmental health risk perception, Students, Public health, Functional health literacy, Risk communication
Abstract

Among the determinants of environmental health risk perception, health literacy and social media messages have been generally neglected. This study details the environmental health risk perception and its determinants in Italian university students, including a measure of functional health literacy and an analysis of newspapers and social media. A cross sectional survey was carried out among students from 15 Italian universities and different disciplines (grouped into Scientific-Health and Humanistic-Legal-Social sectors) using a self-administered anonymous questionnaire, divided into six sections: socio-demographic characteristics, information on health and environment, environmental health risk perception, trust, attitudes and behaviors and functional health literacy. Local newspapers and tweets in the same areas and period were analyzed in relation to quantity and topics. The study population included 4778 students (65.1% female) aged 21 ± 4.3 years, and functional health literacy was low (below the cutoff value) for 44.4% of students. A new outcome of the survey is that the detected association between high functional health literacy a higher global health risk perception and trust in institutions both as sources of information and as actors for protection against environmental risks. The internet and social networks were the most frequently consulted sources of information (77.7%), which was predictive of a higher risk perception. The possible relation between environmental health risk perception and tweet communication was highlighted by a comparison between the risk perception in the city with the highest number of tweets (Modena) and another one similar for socio-demographic characteristics (Pisa). In conclusion, the results of our study may be of help to strengthen information and education programs: functional health literacy should be taken into account in school programs, to produce a basic knowledge for a better understanding of health and environment. Moreover, mass and social media should be included in planning communication intervention and in verifying their results.

Published
2019

43. Molecular identification and echinocandin susceptibility of candida parapsilosis complex bloodstream isolates in Italy, 2007-2014

Author

Stella Balbino, Giulia Morace, Federica Perdoni, Elisa Borghi, Osvalda De Giglio, G Lovero, Giuseppina Caggiano, Daniela Cirasola, Maria Teresa Montagna, Lovero, G, Borghi, E, Balbino, S, Cirasola, D, De Giglio, O, Perdoni, F, Caggiano, G, Morace, G, and Montagna, M

Subjects
0301 basic medicine, Antifungal Agents, lcsh:Medicine, Fungal Protein, Yeast and Fungal Models, Artificial Gene Amplification and Extension, Pathology and Laboratory Medicine, Candida parapsilosis, Pediatrics, Polymerase Chain Reaction, Geographical Locations, Hospitals, University, Echinocandins, chemistry.chemical_compound, Medicine and Health Sciences, Antifungal Agent, Prospective Studies, lcsh:Science, Deoxyribonucleases, Type II Site-Specific, Candida, Fungal Pathogens, Fungal protein, Cross Infection, Multidisciplinary, biology, Antimicrobials, Microbial Sensitivity Test, Broth microdilution, Drugs, Hospitals, Europe, Intensive Care Units, Italy, Medical Microbiology, Pathogens, Pediatric Infections, Polymorphism, Restriction Fragment Length, Research Article, medicine.drug, Human, Echinocandin, 030106 microbiology, Genes, Fungal, Mycology, Microbial Sensitivity Tests, Research and Analysis Methods, Microbiology, Antibiotic Susceptibility Testing, Alcohol Oxidoreductase, Fungal Proteins, 03 medical and health sciences, Model Organisms, Species Specificity, Microbial Control, parasitic diseases, medicine, Candida Albicans, Humans, Molecular Biology Techniques, Microbial Pathogens, Molecular Biology, Etest, Pharmacology, Antifungals, lcsh:R, Organisms, Fungi, Micafungin, Biology and Life Sciences, Candidemia, bacterial infections and mycoses, biology.organism_classification, Yeast, Health Care, Alcohol Oxidoreductases, Prospective Studie, Pharmacologic Analysis, chemistry, Health Care Facilities, People and Places, Anidulafungin, lcsh:Q, Caspofungin
Abstract

The Candida parapsilosis group encompasses three species: C. parapsilosis, C. orthopsilosis, and C. metapsilosis. Here, we describe the incidence and echinocandin susceptibility profile of bloodstream isolates of these three species collected from patients admitted to an Italian university hospital from 2007 to 2014. Molecular identification of cryptic species of the C. parapsilosis complex was performed using polymerase chain reaction amplification of the gene encoding secondary alcohol dehydrogenase, followed by digestion with the restriction enzyme BanI. Minimum inhibitory concentrations were determined using the broth microdilution method according to European Committee for Antimicrobial Susceptibility Testing (EUCAST EDef 7.2) and Clinical Laboratory Standards Institute (CLSI M27-A3) guidelines, and the results were compared with those obtained using the E-test and Sensititre methods. Of the 163 C. parapsilosis complex isolates, 136 (83.4%) were identified as C. parapsilosis, and 27 (16.6%) as C. orthopsilosis. The species-specific incidences were 2.9/10,000 admissions for C. parapsilosis and 0.6/10,000 admissions for C. orthopsilosis. No resistance to echinocandins was detected with any of the methods. The percent essential agreement (EA) between the EUCAST and E-test/Sensititre methods for anidulafungin, caspofungin, and micafungin susceptibility was, respectively, as follows: C. parapsilosis, 95.6/97.8, 98.5/88.2, and 93.4/96.3; C. orthopsilosis, 92.6/92.6, 96.3/77.8, and 63.0/66.7. The EA between the CLSI and E-test/Sensititre methods was, respectively, as follows: C. parapsilosis, 99.3/100, 98.5/89.0, and 96.3/98.5; C. orthopsilosis, 96.3/92.6, 100/81.5, and 92.6/88.9. Only minor discrepancies, ranging from 16.9% (C. parapsilosis) to 11.1% (C. orthopsilosis), were observed between the CLSI and E-test/Sensititre methods. In conclusion, this epidemiologic study shows a typical C. parapsilosis complex species distribution, no echinocandin resistance, and it reinforces the relevance of using commercially available microbiological methods to assess antifungal susceptibility. These data improve our knowledge of the national distribution of species of the psilosis group, as there are very few studies of these species in Italy.

Published
2016

44. A systematic scoping review of antibiotic-resistance in drinking tap water.

Author

Federigi I, Bonetta S, Tesauro M, De Giglio O, Oliveri Conti G, Atomsa NT, Bagordo F, Bonetta S, Consonni M, Diella G, Ferrante M, Grasso A, Macrì M, Montagna MT, Verani M, and Carducci A

Abstract

Environmental matrices have been considered of paramount importance in the spread of antibiotic-resistance; however, the role of drinking waters is still underexplored. Therefore, a scoping review was performed using a systematic approach based on PRISMA guidelines, with the aim of identifying and characterizing antibiotic-resistance in tap water, specifically, water treated at a potabilization plant and provided for drinking use through a water distribution system. The review included 45 studies, the majority of which were conducted in upper-middle-income economies (42.2%), mainly from the Western Pacific region (26.7%), followed by Europe (24.4%). Most of the papers focused on detecting antibiotic-resistant bacteria (ARB), either alone (37.8%) or in combination with antibiotic-resistant genes (ARGs) (26.7%). Multidrug-resistance profile was often identified in heterotrophic bacteria, including various species of nontuberculous mycobacteria, Pseudomonas spp., and Aeromonas spp., which were especially resistant to penicillins, cephalosporins (including 3rd-generation), and also to macrolides (erythromycin) and tetracyclines. Resistance to a wide range of antibiotics was also prevalent in fecal bacteria, e.g., the Enterobacteriaceae family, with common resistance to (fluoro)quinolones and sulfonamide groups. ARGs were investigated either in bacterial strains isolated from tap waters or directly in water samples, and the most frequently detected ARGs belonged to β-lactam, sulfonamide, and tetracycline types. Additionally, mobile genetic elements were found (i.e., int1 and tnpA). Sulfonamides and macrolides were the most frequently detected antibiotics across countries, although their concentrations were generally low (<10 ng/L) in Europe and the United States. From a health perspective, tap water hosted ARB of health concern based on the 2024 WHO bacterial priority pathogens list, mainly Enterobacteriaceae resistant to 3rd-generation cephalosporin and/or carbapenem. Despite the fact that tap water is treated to meet chemical and microbiological quality standards, current evidence suggests that it can harbor antibiotic-resistance determinants, thus supporting its potential role in environmental pathways contributing to antibiotic resistance., Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2024 The Authors. Published by Elsevier Inc. All rights reserved.)

Published
2024
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45. Occurrence of Uncultured Legionella spp. in Treated Wastewater Effluent and Its Impact on Human Health (SCA.Re.S Project).

Author

De Giglio O, Diella G, Bagordo F, Savino AF, Calabrese A, Campanale M, Triggiano F, Apollonio F, Spagnuolo V, Lopuzzo M, Grassi T, Caputo MC, Brigida S, Valeriani F, Romano Spica V, and Montagna MT

Abstract

Wastewater treatment plants (WWTPs) provide optimal conditions for the environmental spread of Legionella . As part of the Evaluation of Sanitary Risk Related to the Discharge of Wastewater to the Ground (SCA.Re.S) project, this study was conducted to evaluate the presence of Legionella in WWTP effluent and in groundwater samples collected from two wells located downstream from the plant. The samples were analyzed to determine the concentrations of Legionella spp using the standard culture-based method and molecular techniques, followed by genomic sequencing analysis. Legionella was detected only with the molecular methods (except in one sample of effluent positive for L. pneumophila serogroup 6), which showed viable Legionella pneumophila and L. non- pneumophila through the use of free DNA removal solution in both the effluent and groundwater, with concentrations that progressively decreased downstream from the plant. Viable L. pneumophila appeared to be slightly more concentrated in warm months. However, no significant differences ( p ≥ 0.05) in concentrations between cold and warm months were observed. A genotypic analysis characterized the species present in the samples and found that uncultured Legionella spp, as yet undefined, constituted the prevalent species in all the samples (range 77.15-83.17%). WWTPs play an important role in the hygienic and sanitary quality of groundwater for different uses. The application of Legionella control systems during the purification of effluents is warranted to prevent possible outbreaks of legionellosis.

Published
2024
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46. The Spread of Mycobacterium chimaera from Heater-Cooler Units and Infection Risk in Heart Surgery: Lessons from the Global Outbreak?

Author

Spagnolo AM, De Giglio O, Caggiano G, D'Agostini F, Martini M, Orsini D, and La Maestra S

Abstract

Mycobacterium chimaera (MC), a member of the Mycobacterium avium complex, can cause infections in patients after open-heart surgery due to contaminated heater-cooler units (HCUs). The transmission route of HCU-related MC infection is non-inhalational, and infection can occur in patients without previously known immune deficiency. Patients may develop endocarditis of the prosthetic valve, infection of the vascular graft, and/or manifestations of disseminated mycobacterial infection (splenomegaly, arthritis, hepatitis, nephritis, myocarditis, etc.). MC infections have serious outcomes (30-50% recurrence rate, 20-67% mortality rate). In 2015, an international outbreak of M. chimaera infections among patients undergoing cardiothoracic surgeries was associated with exposure to contaminated LivaNova 3T HCUs (formerly Stöckert 3T heater-cooler system, London, United Kingdom). In response to the global outbreak, many international agencies have issued directives and recommendations in order to reduce the risk of MC infection in cardiac surgery. Whole-genome sequencing (WGS) technology can be used to describe the global spread and dynamics of MC infections, to characterize local outbreaks, and also to identify sources of infection in hospital settings. In order to minimize the risk of contamination of HCUs and reduce the risk of patient infection, it is imperative that healthcare facilities establish a program of regular cleaning and disinfection maintenance procedures as well as monitoring of the water used and the air in the operating room, in accordance with the manufacturer's procedure.

Published
2024
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47. In Vitro Virucidal Activity of Different Essential Oils against Bovine Viral Diarrhea Virus Used as Surrogate of Human Hepatitis C Virus.

Author

Lanave G, Pellegrini F, Triggiano F, De Giglio O, Lucente MS, Diakoudi G, Catella C, Gentile A, Tardugno R, Fracchiolla G, Martella V, and Camero M

Abstract

The hepatitis C virus (HCV) is a major hepatotropic virus that affects humans with increased risk of developing hepatocellular carcinoma. The bovine viral diarrhea virus (BVDV) causes abortion, calf mortality and poor reproductive performance in cattle. Due the difficulties of in vitro cultivation for HCV, BVDV has been used as surrogate for in vitro assessment of the efficacy of antivirals. Essential oils (EOs) display antiviral and virucidal activity on several viral pathogens. In this study, the virucidal activity of five EOs, Salvia officinalis L. EO (SEO), Melissa officinalis L. EO (MEO), Citrus lemon EO (LEO), Rosmarinus officinalis L. EO (REO) and Thymus vulgaris L. EO (TEO) against BVDV was evaluated in vitro at different concentrations for several time contacts. MEO and LEO were able to considerably inactivate BVDV with a time- and dose-dependent fashion. MEO and LEO at the highest concentrations decreased viral titer by 2.00 and 2.25 log 10 TCID 50 /50 μL at 8 h contact time, respectively. SEO, REO and TEO displayed mild virucidal activity at the highest concentrations for 8 h contact times. In this study, the virucidal efficacies of MEO and LEO against BVDV were observed regardless of compound concentration and contact time. Further studies are needed to confirm the potential use of MEO and LEO as surface disinfectants.

Published
2024
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48. Mechanism of surfactant interactions with feline coronavirus: A physical chemistry perspective.

Author

Mateos H, Mallardi A, Camero M, Lanave G, Catella C, Buonavoglia A, De Giglio O, Buonavoglia C, and Palazzo G

Subjects
Animals, Cats, Sodium Dodecyl Sulfate, Virus Inactivation, Surface-Active Agents pharmacology, Surface-Active Agents chemistry, Coronavirus, Feline physiology
Abstract

Hypothesis: Surfactants are inexpensive chemicals with promising applications in virus inactivation, particularly for enveloped viruses. Yet, the detailed mechanisms by which surfactants deactivate coronaviruses remain underexplored. This study delves into the virucidal mechanisms of various surfactants on Feline Coronavirus (FCoV) and their potential applications against more pathogenic coronaviruses., Experiments: By integrating virucidal activity assays with fluorescence spectroscopy, dynamic light scattering and laser Doppler electrophoresis, alongside liposome permeability experiments, we have analyzed the effects of non-ionic and ionic surfactants on viral activity., Findings: The non-ionic surfactant octaethylene glycol monodecyl ether (C 10 EO 8 ) inactivates the virus by disrupting the lipid envelope, whereas ionic surfactants like Sodium Dodecyl Sulfate and Cetylpyridinium Chloride predominantly affect the spike proteins, with their impact on the viral membrane being hampered by kinetic and thermodynamic constraints. FCoV served as a safe model for studying virucidal activity, offering a faster alternative to traditional virucidal assays. The study demonstrates that physicochemical techniques can expedite the screening of virucidal compounds, contributing to the design of effective disinfectant formulations. Our results not only highlight the critical role of surfactant-virus interactions but also contribute to strategic advancements in public health measures for future pandemic containment and the ongoing challenge of antimicrobial resistance., Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2024 The Author(s). Published by Elsevier Inc. All rights reserved.)

Published
2024
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49. Factors Influencing Microbial Contamination of Groundwater: A Systematic Review of Field-Scale Studies.

Author

Bagordo F, Brigida S, Grassi T, Caputo MC, Apollonio F, De Carlo L, Savino AF, Triggiano F, Turturro AC, De Donno A, Montagna MT, and De Giglio O

Abstract

Pathogenic microorganisms released onto the soil from point or diffuse sources represent a public health concern. They can be transported by rainwater that infiltrates into subsoil and reach the groundwater where they can survive for a long time and contaminate drinking water sources. As part of the SCA.Re.S. (Evaluation of Health Risk Related to the Discharge of Wastewater on the Soil) project, we reviewed a selection of field-scale studies that investigated the factors that influenced the fate of microorganisms that were transported from the ground surface to the groundwater. A total of 24 studies published between 2003 and 2022 were included in the review. These studies were selected from the PubMed and Web of Science databases. Microbial contamination of groundwater depends on complex interactions between human activities responsible for the release of contaminants onto the soil, and a range of environmental and biological factors, including the geological, hydraulic, and moisture characteristics of the media traversed by the water, and the characteristics and the viability of the microorganisms, which in turn depend on the environmental conditions and presence of predatory species. Enterococci appeared to be more resistant in the underground environment than thermotolerant coliforms and were suggested as a better indicator for detecting microbial contamination of groundwater.

Published
2024
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50. The Presence of Legionella in Water Used for Car Washing: Implications for Public Health.

Author

Laganà P, Facciolà A, Palermo R, De Giglio O, Delia SA, and Gioffrè ME

Abstract

Although today all of the aspects of Legionella are better understood than in the past, in many countries the interest is still mainly focused on healthcare and tourism facilities. Other at-risk areas are less explored, such as those where workers are often in contact with water during their activities. In reality, any water system capable of producing aerosols can be considered a potential source of Legionella transmission, including car washes, where a large number of users work and flow through annually. From January to May 2022, 120 samples were carried out in 30 car washes located in Messina (Italy): 60 samples of water and 60 of aerosols. The aim of this investigation was to evaluate the risk of legionellosis in car washing workers exposed to potentially contaminated aerosols. To increase the probability of finding Legionella , the sample collections were organized on different days of the week. Of the total samples taken, 10 (8.3%) were positive for Legionella : seven (11.7%) water (range 100-1000 CFU) and three (5%) aerosol (range 10-150 CFU) samples. Detected serogroups were L. pneumophila sgr 1, 7, 10 and Legionella gormanii . Given the results obtained, preventative measures should be implemented in such facilities in order to protect the health of users and car wash operators.

Published
2023
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