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目的本研究旨在对RhD阳性个体中红细胞特异性表达的RHD基因不同转录本进行分析，并对选择性剪接发生的机制进行初步探讨。方法利用体外有核红细胞培养体系分离并培养RhD阳性个体的有核红细胞，使用逆转录聚合酶链反应（RT-PCR）技术对RhD阳性个体RHD基因外显子6至3’非编码区扩增后进行二代测序，分析mRNA转录本，并采用生物信息学方法分析RHD基因所有外显子5’及3’剪接位点的最大熵值。结果RHD基因的转录本以正常全长mRNA为主，除此之外还存在其他8种异常转录本。按照表达量从多到少，分别为外显子7缺失、外显子9缺失、外显子8和9缺失、外显子7、8和9缺失、外显子7和9缺失、外显子8和9缺失合并外显子7与10间插入一段170 bp的内含子7片段、全长mRNA但插入170 bp的内含子7片段和外显子9缺失但插入一170 bp的内含子7片段，其中最后3种为新转录本。生物信息学分析提示外显子7和外显子9异常剪接可能与其5’-剪接位点（5’-splice site，5’ss）或3’ss和剪接体结合能力下降有关，而内含子7片段异常表达于转录本中可能与其两端存在类似5’ss和3’ss剪接保守序列有关。结论RHD基因存在十分复杂的选择性剪接模式，使RhD阳性个体的RHD基因在mRNA水平上存在多种不同的剪接转录本。

【目的】探讨特殊O型个体的血清学特征和分子背景。【方法】收集ABO正反定型结果不一致的O 型标本12例，采用试管法进行正反定型、H抗原物质检测、吸收放散等实验；PCR扩增ABO基因编码区外显子1- 7，并进行直接测序；对于新发现的突变位点进行克隆测序；利用PDBviewer软件构建GTB酶蛋白突变体的3D模 型，并对突变引发的GTB酶结构和功能变化进行分析。【结果】12例标本正定型均为O型，反定型抗-A减弱的2 例、完全消失的3例，抗-B减弱的5例、完全消失的2例；红细胞与抗-H的凝集强度均≥ 3+；所有标本均不能吸 收放散抗-A和抗-B；等位基因ABO*O01、ABO*O02、ABO*O06、ABO*O13在该类人群中出现频率较高；发现2例 Ael02/O02基因型的个体；1条等位基因所含c.563G > A（p.R188H）为新的突变位点，3D模型表明该突变位于GTB 酶的底物结合区，突变引起R188的氢键数目减少。【结论】特殊O型的血清学特征以血浆中的单个抗体减弱或 消失为主；ABO*O01、ABO*O02、ABO*O06、ABO*O13是这类血型个体的主要等位基因；新的等位基因ABO*O77 可通过影响GTB酶的活性导致B抗原的生成缺失。

Copyright of Metal Working (1674-165X) is the property of Metal Working Editorial Office and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

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Copyright of Journal of Modern Medicine & Health is the property of Journal of Modern Medicine & Health and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Copyright of China Tropical Medicine is the property of China Tropical Medicine Editorial Office and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Copyright of Journal of Nanchang University (Natural Science) is the property of Journal of Nanchang University (Natural Science) Editorial Office and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Zinc telluride (ZnTe) nonlinear crystal, a commonly used electro-optical material, can be used to generate and detect Terahertz (THz) wave, and has been widely used in the fields of THz wave detection and imaging. However, the preparation of ZnTe crystal with high quality and large size still remains a challenge. In this paper, ZnTe single crystal rod with diameter of 40 mm and length of 140 mm was successfully prepared by the pressure assisted vertical Bridgman method. The crystal structure, crystalline quality, optical and electrical properties of ZnTe crystal were characterized and analyzed. The effects of annealing process of ZnTe crystal on the crystal's properties related to Te inclusions were investigated. According to the research results, after annealed at 850 ℃ for 200 h under Zn atmosphere, the transmittance of the crystal in the visiblenear infrared wave band reaches up to 62%, the resistivity of the crystal is 104 Ω·cm, and the absorption coefficient is from 5 cm-1 to 15 cm-1 at the range from 0. 2 THz to 2 THz. The results provide a valuable reference for the preparation of highquality ZnTe crystal. [ABSTRACT FROM AUTHOR]

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Copyright of China Brewing is the property of China Brewing Editorial Office and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Copyright of Journal of Nanjing University of Information Science & Technology (Natural Science Edition) / Nanjing Xinxi Gongcheng Daxue Xuebao (ziran kexue ban) is the property of Editorial Office of Journal of Nanjing University of Information Science & Technology and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

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Copyright of Science & Technology of Cereals, Oils & Foods is the property of Science & Technology of Cereals, Oils & Foods Editorial Office and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

研究针对性护理干预措施在计算机断层扫描(CT)增强扫描中对高速注射碘对比剂患者 的应用效果。方法 选取该院2019年3月至2021年3月收治的104例接受CT增强扫描患者作为研究对象, 随机将患者分为对照组和观察组,每组各52例。对照组患者在接受CT检查的过程中采用常规护理干预,观察 组则在接受CT检查的过程中实施针对性护理干预。对比2组患者不良反应发生情况、检查舒适度、护理满意 度。结果 观察组渗液、喉头水肿、恶心呕吐等不良反应总发生率低于对照组,差异均有统计学意义(P <0. 05)。观察组检查过程中的舒适度评分均高于对照组,差异有统计学意义(P <0.05)。观察组护理满意度为 98.08%(51/52),高于对照组的82.69%(43/52),差异有统计学意义(P <0.05)。结论 在增强CT 扫描检查 中开展针对性护理,能有效降低不良反应风险,提升患者舒适度和护理满意度。 [ABSTRACT FROM AUTHOR]

Copyright of China Rural Water & Hydropower is the property of China Rural Water & Hydropower Editorial Office and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

At the 30th anniversary of their dialogue relations, China and ASEAN agreed to upgrade their relationship to a comprehensive strategic partnership for peace, security, prosperity and sustainable development. China-ASEAN relations have been a cornerstone for the post-Cold War peace and prosperity in East Asia and the core for Asian regionalism. Under the three guiding principles of creating strategic opportunities, constructing developmental peace, and adhering to ASEAN centrality, their relationship has been dynamic and substantive. As the China-ASEAN relationship becomes increasingly asymmetric and China-US interaction in the region becomes more competitive in the years after the 2008 global financial crisis, ASEAN has developed mixed feelings and perceptions about China’s growing power and influence in the area. In particular, ASEAN has felt anxious about the impact of intensifying geopolitical competition on the regional cooperation process and ASEAN centrality. Since the outbreak of COVID-19, ASEAN has realized the urgency of strengthening its own cohesiveness, promoting major-power cooperation, and addressing non-traditional security threats such as infectious diseases to achieve post-pandemic economic recovery and sustainable growth. To build a future-oriented comprehensive strategic partnership, China should exercise restraint, shoulder responsibility, and work with ASEAN as equal partners to create a consensus-based regional order. [ABSTRACT FROM AUTHOR]

Copyright of Food & Machinery is the property of Food & Machinery Editorial Office and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Copyright of Chemistry & Industry of Forest Products is the property of Chemistry & Industry of Forest Products Editorial Department and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Copyright of Chinese Journal of Contemporary Pediatrics is the property of Xiangya Medical Periodical Press and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Copyright of Journal of Frontiers of Computer Science & Technology is the property of Beijing Journal of Computer Engineering & Applications Journal Co Ltd. and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Copyright of Journal of Xi'an Jiaotong University (Medical Sciences) is the property of Xian Jiaotong University and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Copyright of Journal of Jiangsu University (Natural Science Edition) / Jiangsu Daxue Xuebao (Ziran Kexue Ban) is the property of Editorial Department of Journal of Jiangsu University (Natural Science Edition) and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

[Objectives] This study was conducted to explore the effect of fruit load per eluster on fruit quality, aroma components and relevant gene expression levels in 'Shine Museat' grape. [Methods] Taking the 'Shine Museat' as experimental material, berry thinning was conducted to leave 40 ( \M), 60( 1.60), 80( 1.80) berry numben ; per cluster at one week after flowering. The aroma eomponent and contents at different development stages i in grape were thoroughly: determined by GC-MS, and the expression of relevant genes in the grape at different development stages were determined by qRT-PCR.[Results]The effect of different fruit loads on the appearanee of grape fruit was obvious. 1-40 treatment was superior uill i graceful shape, full fruit and uniform color; 1-60 treatment ineluded smaller berries and sparse cluster ; 1.80 treatment reac hed ripe inconsistency and mixed with different size fruit. ITie soluble solid contents of 1.40 treatment were higr ier, and titrate acid contents were lowest with significant difference. At the maturity stage, C6 compound eontents of 1.60 treatment were higher, but for the total terpene contents, L40 treatment was the highest, and especially for linalool and geraniol. In addition, the terpene compounds of 1.40 treatment dropped more slowly, and several trace terpcnes such as cis-lina]oloxide, citronello] disappeared later. The transcriptional analysis of the relative genes revealed that alcoholdehy drogenase gene( VvADH) was mainly responsible for alcohols accumulation. The expression of deoxyxylulose 5-phosphate synthase gene(VvDXS) was significantly affected by the treatment of different fruit loads, and correlated with the total content of terpenes. Overall the expression of VvDXS in L40 treatment was higher th. an others. [Conclusions] The treatment of 40 berry numbers was conductive to the accumulation and preservation of aroma compounds at the late stage of fruit development, and could significantly improve the appearance quality of the fruit. [ABSTRACT FROM AUTHOR]

Copyright of Journal of Xi'an Jiaotong University (Medical Sciences) is the property of Xian Jiaotong University and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Concerning the characteristics of order picking in storage center，such as the large transportation amount， the time-tight in searching and the high accuracy of picking， RFID technology has been used to realize automatic identification of warehousing，location, picking and delivery of goods in warehouse operation， which can achieve accuracy and timely update the information of cargoes and order in the database and reduce the time of goods search in order. All of this can realize the storage center’s visual management. Further，the deviation ratio was applied to optimize the picking process in complicated two-block warehouses’s picking， which can give different picking paths correspond to the different locations of the picking equipment. Finally， through the contrast with the traditional strategy and S-shaped heuristic method，the stimulation results show that the operation is remarkably optimized and the warehousing picking quality and efficiency are improved. [ABSTRACT FROM AUTHOR]

Objective To explore the long-term therapeutic effects of co-transplantation of Sprague-Dawley (SD) rats islets and bone marrow mesenchymal stem cells (BMSCs) to treat type 1 diabetes mellitus (T1DM). Methods BMSCs were isolated from 1 week of age of 4 healthy male SD rats while islets were isolated from 6-8 weeks of age of 3 healthy male SD rats BMSCs and islets were both cultured with 1640 nutrient medium in either separately or together in vitro. The cell morphology, levels of insulin and C-peptide in each group were examined at the 1st, 3rd, and 7th day after culture. T1DM model (male SD rats, body weight 180-200 g) was established by administration of 1% streptozotocin (STZ) citrate buffer (60 mg/kg) through intraperitoneal injection. T1DM model rats were randomly divided into 4 groups: model group, BMSCs transplantation group, islets transplantation group, and co-transplantation group (15 rats each group) for the cell transplantation test in vivo. The fasting glucose, body weight, insulin, C-peptide, total bilirubin (TBIL), aspartate transaminase (AST) and alanine aminotransferase (ALT) of each group were examined at the 1st, 3rd, 7th, 14th, and 30th day after transplantation. Data between multigroups were analyzed by ANOVA, and data between two groups were analyzed by t test. Results Compared to islets transplantation group, blood glucose in co-transplantation group's was lower (t=11.44-28.02, P<0.05), and insulin (t=10.03-6.86, P<0.05) and C-peptide (t=1.47-2.59, P<0.05). However, the TBIL (t=7.67-6.39, P<0.05), AST (t=12.00-8.89, P<0.05), and ALT (t=8.08-10.40, P<0.05) in co-transplantation group were lower than those in islets transplantation group. The expression intensity of insulin was stronger in co-transplantation group than islets transplantation group. The differences of above indexes between model group and BMSCs group had no statistical significance (all P>0.05). Conclusion Compared with islets transplantation alone, islets and BMSCs co-transplantation have better therapeutic effects on T1DM. [ABSTRACT FROM AUTHOR]

Copyright of Tourism Tribune / Lvyou Xuekan is the property of Tourism Institute of Beijing Union University and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Copyright of Journal of Beijing University of Traditional Chinese Medicine is the property of Journal of Beijing University of Traditional Chinese Medicine and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Aiming at frequently appear data redundancy and data reusable inefficiency problems, this paper combined the column-storage mechanism with parallel processing to optimize data reuse strategy. It built a parallel processing model based on MapReduce of data reuse, and used the improved pattern matching algorithm CSM combine the data screening algorithm to propose parallel data reuse algorithm. This algorithm used the pattern matching algorithm to determine the correspondence between the attribute columns, and through data detected method verifies the feasibility of reusing data attribute columns, thereby filtered the data columns and realized the parallel data reuse strategy. Under the big data, it used the data tables of large scale data sets SSB and TPCH in data warehouse to experiment. The results of storage and treatment time are decreased by 17% and 35% , and verified parallel data reuse strategy has more optimized than the general strategy in data storage and data processing time. [ABSTRACT FROM AUTHOR]

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Copyright of Journal of Northeastern University (Social Science) / Dongbei Daxue Xuebao (Shehui Kexue Ban) is the property of Dongbei Daxue and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Copyright of Journal of Beijing University of Traditional Chinese Medicine is the property of Journal of Beijing University of Traditional Chinese Medicine and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

OBJECTIVE: To investigate the characteristics and regularities of adverse drug reactions (ADR) in Jiangsu Donghai County People’s Hospital (hereinafter referred to as “our hospital”) , so as to provide reference for clinical rational drug application. METHODS: Retrospectively analysis was conducted on 314 cases of ADR reports in terms of types, patients’ genders and ages, ADR-inducing administration routes and drugs, ADR involved organs and (or) systems and its main clinical features, ADR prognoses and the relevance evaluations of ADR. RESULTS: Among the 314 reports of ADR, 171 cases were general ADR (54. 46% ) , 41 cases were severe ADR ( 13. 06% ) , 102 cases were new and severe ADR (32.48%); the number of male patients was slightly more than that of females; most patients aged ≤ 3; most ADR were induced by intravenous drip, the second was oral administration and intravenous injection. 86 kinds of 11 categories of drugs were involved in the 314 reports of ADR, the top 3 ADR-inducing drugs were respectively antibiotics, traditional Chinese medicine and drugs for nervous system; among reports of ADR induced by antibiotics, the top 3 were respectively cephalosporins, macrolides and fluoroquinolones. ADR mainly involved skin and its accessories, the second were digestive system and circulatory system. Of the relevance evaluations, 80 cases were “definitely” (25.48%), 52 cases were “possible” (16.56%), 182 cases were “probably” (57.96%). All patients were cured or went better after treatment. CONCLUSIONS: The occurrence of ADR was related to various factors, ADR reporting and monitoring on medication should be reinforced; clinical pharmacists should lake active participation in clinical treatment to promote rational medication. [ABSTRACT FROM AUTHOR]

OBJECTIVE: To investigate the characteristics and laws of adverse drug reaction (ADR) in People's Hospital of Jiangsu Donghai County (hereinafter referred to as"our hospital"), and to provide reference for the rational drug use in clinic. METHODS: Retrospective analysis was conducted on 202 cases of ADR reports that were reported to China's National Center for ADR Monitoring by our hospital during 2013-2014, in terms of patients' age and gender, categories of ADR-inducing drugs, ADR-involved organs and (or) systems and clinical manifestations. RESULTS: Of the 202 cases, the age span was large, from 1 d ~ 84 years old. Male to female ratio was 1 ∶ 3. 5. And the main administration route was intravenous administration, totally 194 cases (96. 04%); 10 types of drugs were involved, among which 119 cases (58. 91%) were anti-infective drugs, followed by traditional Chinese preparation, totally 43 cases (21. 29%). 97 cases (48. 02%) with skin and its appendages impairment took the lead, followed by 38 cases (18. 81%) with digestive system damage. CONCLUSIONS: It is necessary to strengthen the ADR monitoring and disseminate ADR knowledge, so as to ensure the medication safety in patients. [ABSTRACT FROM AUTHOR]

Objective To investigate the regulatory function of transforming growth factor (TGF)-β in transplantation immune tolerance induced by anti-CD45 RB antibody or in combination with anti-Tim-1 antibody. Methods Mouse models with allogeneic cardiac and pancreatic islet transplantation were established. The animals with allogeneic cardiac transplantation were treated with anti-CD45 RB antibody and (or) anti- TGF-β antibody and those with pancreatic islet transplantation were handled with anti-CD45 RB antibody in combination with anti-Tim-1 antibody supplemented with /without anti-TGF-β antibody. The long-term survival of transplant graft was observed. B cells from recipients which tolerated by pancreas islet transplantation for long time,were isolated and subsequently infused into the mouse models with islet cell transplantation. The survival of pancreas islet transplant graft was observed after anti-TGF-β antibody therapy. After doubleantibody treatment in vitro for 14 d,B cell from recipients were mingled with lipopolysaccharide (LPS) and cultured overnight. The percentage of CD19+LAP+cell was quantitatively measured by flow cytometer analysis. Results Six among 9 mice with allogeneic cardiac transplantation survived for a long term after anti-CD45 RB antibody therapy. All cardiac grafts developed transplant rejection if use of anti-TGF-β antibody was supplemented. In mouse models with islet cell transplantation,combined use of anti-CD45 RB and anti-Tim-1 antibodies induced long-term immune tolerance in 90% of transplant grafts. No transplant graft survived for a long period after anti-TGF-β antibody was delivered. Following B cell infusion,8 of 9 pancreas islet grafts induced no transplant rejection,whereas yielded transplant rejection immediately after supplementing anti-TGF-β antibody. B cells were stimulated and cultured in vitro. Flow cytometry analysis revealed the proportion of CD19+LAP+cells in B cells was significantly elevated (P < 0. 0001). Conclusions Immune tolerance induced by anti-CD45 RB antibody or in combination with anti-Tim-1 antibody depends on TGF-β,probably regulated by regulatory B cells. [ABSTRACT FROM AUTHOR]