14 results on '"Qiu, Jian"'
Search Results
2. Transcriptionally amplified synthesis of fluorogenic RNA aptamers for label-free DNA glycosylase assay.
- Author
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Ma, Fei, Liu, Ya-Zhen, Liu, Meng, Qiu, Jian-Ge, and Zhang, Chun-Yang
- Subjects
RNA synthesis ,DNA ,DETECTION limit ,APTAMERS - Abstract
We demonstrate for the first time the utilization of fluorogenic RNA aptamers for label-free uracil-DNA glycosylase (UDG) assay. Through rationally engineering the transcription machine with dU substitution, this assay requires only a single probe to simultaneously sense and amplify the UDG signal, achieving a low detection limit of 6.3 × 10
−6 U mL−1 . Moreover, it can be applied for screening UDG inhibitors and measuring endogenous UDG activity in different cells. [ABSTRACT FROM AUTHOR]- Published
- 2022
- Full Text
- View/download PDF
3. Behaviour of spirotetramat residues and its four metabolites in citrus marmalade during home processing
- Author
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Qiu Jian, Dali Sun, Xuesu Su, Lei Gong, Weijun Chen, Bining Jiao, Yanyu Liu, and Liyan Jiang
- Subjects
Citrus ,Food Handling ,Health, Toxicology and Mutagenesis ,engineering.material ,Toxicology ,01 natural sciences ,0404 agricultural biotechnology ,Tandem Mass Spectrometry ,Liquid chromatography–mass spectrometry ,Spiro Compounds ,Food science ,Chromatography, High Pressure Liquid ,Detection limit ,Aza Compounds ,Chromatography ,Molecular Structure ,Chemistry ,Pulp (paper) ,010401 analytical chemistry ,Pesticide Residues ,Public Health, Environmental and Occupational Health ,food and beverages ,04 agricultural and veterinary sciences ,General Chemistry ,General Medicine ,040401 food science ,0104 chemical sciences ,engineering ,Spirotetramat ,Environmental Monitoring ,Food Science ,Field conditions ,Citrus fruit - Abstract
The effect of home processing on the residues of spirotetramat and its four metabolites (B-enol, B-glu, B-mono and B-keto) in citrus marmalade is comprehensively investigated in this paper by ultra-performance liquid chromatography-tandem mass spectrometry (UPLC-MS/MS). A five-fold recommended dose of spirotetramat was applied to citrus fruit under field conditions and the processing included five steps: washing, peeling, pre-treatment for peel, mixing and boiling. The results showed that spirotetramat was the predominant component detected in unprocessed citrus, accounting for 64%. All the detected residues were primarily deposited on citrus peel, except for B-enol which was also present in the citrus pulp. Washing reduced spirotetramat, B-enol, B-glu and B-keto by 83%, 56%, 41% and 16%, respectively, and pre-treatment of the peel removed between 42% and 68% of the residues. Four compounds were all below the limit of detection after the mixing step. In the final product, only B-keto was detected at the concentration of 0.010 mg kg(-1). After the whole process, the processing factors for spirotetramat, B-enol, B-glu and B-keto were < 0.041, < 0.125, < 0.294 and 0.313, respectively, which indicated that home processing can significantly reduce residues of spirotetramat and its metabolites in citrus marmalade.
- Published
- 2016
4. Zirconium ion-mediated assembly of a single quantum dot-based nanosensor for kinase assay.
- Author
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Li, Yueying, Liu, Qian, Cui, Lin, Liu, Wenjing, Qiu, Jian-Ge, and Zhang, Chun-yang
- Subjects
ZIRCONIUM ,PROTEIN kinases ,DETECTION limit ,DIAGNOSIS - Abstract
We demonstrate the zirconium ion-mediated assembly of a single quantum dot (QD)-based nanosensor for accurate detection of protein kinases (PKA) and polynucleotide kinases (PNK). This nanosensor is very sensitive with a detection limit of 8.82 × 10
−4 U mL−1 for PKA and 1.40 × 10−5 U mL−1 for PNK. Moreover, it can be used to analyze the enzyme kinetic parameters and screen the inhibitors of PKA and PNK, with potential applications in drug discovery and clinical diagnosis. [ABSTRACT FROM AUTHOR]- Published
- 2021
- Full Text
- View/download PDF
5. Dissipation and residue of ethephon in maize field
- Author
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Naiwen Jiang, Yong-qiang Ma, Fengmao Liu, Jiannan Dong, and Qiu Jian
- Subjects
Ethylene ,Agriculture (General) ,Plant Science ,maize ,Biochemistry ,S1-972 ,law.invention ,Residue (chemistry) ,chemistry.chemical_compound ,Food Animals ,law ,residue ,Flame ionization detector ,ethephon ,Derivatization ,degradation ,Detection limit ,Potassium hydroxide ,Ecology ,storage stability ,Horticulture ,chemistry ,Agronomy ,Animal Science and Zoology ,Gas chromatography ,Agronomy and Crop Science ,Food Science ,Ethephon - Abstract
A rapid and reliable method was developed for analysis of ethephon residues in maize, in combination with the investigation of its dissipation in field condition and stabilities during the sample storage. The residue analytical method in maize plant, maize kernel and soil was developed based on the quantification of ethylene produced from the derivatization of ethephon residue by adding the saturated potassium hydroxide solution to the sample. The determination was carried out by using the head space gas chromatography with flame ionization detector (HS-GC-FID). The limit of quantification (LOQ) of the method for maize plant was 0.05, 0.02 mg kg−1 for maize kernel and 0.05 mg kg−1 for soil, respectively. The fortified recoveries of the method were from 84.6–102.6%, with relative standard deviations of 7.9–3.8%. Using the methods, the dissipation of ephethon in maize plant or soil was investigated. The half life of ethephon degradation was from 0.6 to 3.3 d for plant and 0.7 to 5.7 d for soil, respectively. The storage stabilities of ethephon residues were determined in fresh and dry kernels with homogenization and without homogenization process. And the result showed that ethephon residues in maize kernels were stable under −18°C for 6 mon. The results were helpful to monitor the residue dissipation of ethephon in the maize ecosystem for further ecological risk assessment.
- Published
- 2015
6. Integration of single-molecule detection with endonuclease IV-assisted signal amplification for sensitive DNA methylation assay.
- Author
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Zhang, Yan, Hu, Jinping, Zou, Xiaoran, Ma, Fei, Qiu, Jian-Ge, and Zhang, Chun-yang
- Subjects
DNA methylation ,ENDONUCLEASES ,DETECTION limit ,CANCER cells ,GENE amplification ,METHYLATION ,BIOSENSORS ,AMPLIFICATION reactions - Abstract
We demonstrate the development of a new fluorescent biosensor for sensitive DNA methylation assay by integrating single-molecule detection with endo IV-assisted signal amplification. This biosensor possesses the characteristics of good selectivity and high sensitivity with a detection limit of 7.3 × 10
−17 M. It can distinguish as low as 0.01% methylation level, and can analyze genomic DNA methylation even in a single cancer cell. [ABSTRACT FROM AUTHOR]- Published
- 2021
- Full Text
- View/download PDF
7. A dumbbell probe-based dual signal amplification strategy for sensitive detection of multiple DNA methyltransferases.
- Author
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Li, Yueying, Sun, Shuli, Tian, Xiaorui, Qiu, Jian-Ge, Jiang, BingHua, and Zhang, Chun-yang
- Subjects
DUMBBELLS ,DNA methyltransferases ,DNA ,DETECTION limit ,ENZYME inhibitors ,METHYLTRANSFERASES ,ADENINE - Abstract
We demonstrate for the first time the integration of a dumbbell probe with dual signal amplification for the simultaneous detection of multiple DNA methyltransferases (MTases). This method is very sensitive with a detection limit of 2.15 × 10
−5 U mL−1 for DNA adenine methyltransferase (Dam) and 3.23 × 10−6 U mL−1 for CpG Methyltransferase (M.SssI), and it can be used to screen the enzyme inhibitors and simultaneously measure Dam and M.SssI in complex biological samples. [ABSTRACT FROM AUTHOR]- Published
- 2020
- Full Text
- View/download PDF
8. Dephosphorylation-directed tricyclic DNA amplification cascades for sensitive detection of protein tyrosine phosphatase.
- Author
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Li, Yueying, Sun, Shuli, Tian, Xiaorui, Qiu, Jian-Ge, Jiang, BingHua, Wang, Li-juan, and Zhang, Chun-yang
- Subjects
PHOSPHOPROTEIN phosphatases ,GENE amplification ,TYROSINE ,DETECTION limit ,CANCER cells ,PROTEIN-tyrosine phosphatase ,FLUORESCENCE - Abstract
We develop a new fluorescence method for the sensitive detection of protein tyrosine phosphatase 1B (PTP1B) based on dephosphorylation-directed tricyclic DNA amplification cascades. This method exhibits good specificity and high sensitivity with a detection limit of 0.24 pM. Moreover, it can be applied for kinetics analysis, inhibitor screening, and the accurate detection of PTP1B in a variety of cancer cells. [ABSTRACT FROM AUTHOR]
- Published
- 2020
- Full Text
- View/download PDF
9. Aggregation-induced fluorescence of the luminol-terbium(III) complex in polymer nanoparticles for sensitive determination of thrombin.
- Author
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Tong, Yuan-Jun, Song, An-Min, Yu, Lu-Dan, Liang, Ru-Ping, and Qiu, Jian-Ding
- Subjects
TERBIUM ,FLUORESCENCE ,POLYMERS ,THROMBIN ,NANOPARTICLES ,DETECTION limit - Abstract
A fluorometric method is described for the determination of thrombin. Polymer nanoparticles containing the luminol-terbium(III) complex (luminol-Tb) were prepared where luminol acts as the bridging ligand, and Tb(III) acts as the central metal ion. Thrombin possesses a large number of electrons donating groups that coordinate with luminol-Tb. Following coordination, the rigidity of the linker is increased, and this decreases the non-radiative decay rate and induces an increase in fluorescence intensity at 430 nm. Hence, thrombin can be fluorometrically determined. The detection limit of thrombin is as low as 3.5 pM (at an SNR of 3). This is about 10 times lower than assays using an aptamer. The method was applied in the determination of thrombin in human serum via the standard addition method and gave satisfying results. [ABSTRACT FROM AUTHOR]
- Published
- 2020
- Full Text
- View/download PDF
10. Colorimetric and electrochemical arsenate assays by exploiting the peroxidase-like activity of FeOOH nanorods.
- Author
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Zhong, Xiao-Li, Wen, Shao-Hua, Wang, Yi, Luo, Yu-Xi, Li, Zhi-Mei, Liang, Ru-Ping, Zhang, Li, and Qiu, Jian-Ding
- Subjects
PEROXIDASE ,ELECTROCHEMICAL analysis ,ARSENATES ,DETECTION limit ,GREEN products ,ELECTRIC conductivity - Abstract
The authors describe an electrochemical and an optical method for the determination of As(V) by using iron oxyhydroxide (FeOOH) nanorods that display peroxidase-mimicking activity. The nanorods catalyze the oxidation of substrate ABTS by H
2 O2 to form a green product with an absorption maximum at 418 nm. If, however, As(V) is electrostatically adsorbed on the nanorods, the oxidation is gradually inhibited. A colorimetric assay was worked out based on these findings. Response is linear in the 0 to 8 ppb and 8 to 200 ppb As(V) concentration range, and the detection limit is 0.1 ppb. Even higher sensitivity is achieved in an electrochemical method which is based on the excellent electrical conductivity of FeOOH nanorods. Electrochemical analysis of As(V) was achieved by first adsorbing As(V) on the nanorods. This inhibits the ABTS reduction current signal, best measured at a potential of 150 mV (vs. Ag/AgCl). The linear range extends from 0.04 to 200 ppb, and the detection limit is as low as 12 ppt. [ABSTRACT FROM AUTHOR]- Published
- 2019
- Full Text
- View/download PDF
11. Ionic covalent organic framework for selective detection and adsorption of TcO4−/ReO4−.
- Author
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Chen, Xiao-Rong, Zhang, Cheng-Rong, Liu, Xin, Liang, Ru-Ping, and Qiu, Jian-Ding
- Subjects
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DETECTION limit , *ADSORPTION (Chemistry) , *CARBAZOLE , *FLUORESCENCE , *SKELETON - Abstract
Herein, a novel fluorescent ionic covalent organic framework (BTTA–BDNP) based on a linked carbazole unit was constructed for the synchronous monitoring and capture of TcO4−/ReO4−. BTTA–BDNP has a fast fluorescence response time with a low detection limit (66.7 nM) for ReO4− (a non-radioactive substitute for TcO4−). Meanwhile, the high charge density and hydrophobic skeleton of BTTA–BDNP enable it to exhibit rapid and selective trapping of ReO4− in complex environments. [ABSTRACT FROM AUTHOR]
- Published
- 2023
- Full Text
- View/download PDF
12. Construction of bidirectional strand displacement-driven three-dimensional DNA walkers for single-molecule monitoring of multiple DNA glycosylases.
- Author
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Liu, Meng, Zhang, Di, Hu, Jin-ping, Wang, Li-juan, Qiu, Jian-Ge, and Zhang, Chun-yang
- Subjects
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DNA glycosylases , *DNA , *GLYCOSYLASES , *SINGLE-stranded DNA , *SIGNAL-to-noise ratio , *DETECTION limit , *EXONUCLEASES - Abstract
Human single-stranded-selective monofunctional uracil glycosylase (hSMUG1) and alkyladenine glycosylase (hAAG) are important base-excision repair (BER) glycosylases responsible for deamination and alkylation repair. Their dysfunctional activities have strong association with various multifactorial diseases and cancers. Herein, by integrating three-dimensional (3D) DNA walker with single-molecule technique, we construct bidirectional strand displacement-driven 3D DNA walkers for single-molecule monitoring of multiple DNA glycosylases. In the presence of hSMUG1 and hAAG, the damaged U and I in bifunctional dumbbell probe are removed by APE1, activating bidirectional self-priming strand displacement amplification (SP-SDA) to produce two trigger DNAs. Trigger DNAs hybridize with Cy5/Alexa Fluor 488 signal probes and subsequently serve as the walker DNAs to induce cyclic APE1-powered cleavage of two signal probes, liberating abundant Cy5 and Alexa Fluor 488 from AuNPs. Due to high precision of intracellular BER mechanisms, high efficiency of bidirectional SP-SDA-directed 3D DNA walkers and ultrahigh signal-to-noise ratio of single-molecule imaging, this nanosensor exhibits a detection limit of 8.14 × 10−10 U/μL for hSMUG1 and 4.50 × 10−9 U/μL for hAAG. Importantly, it can measure kinetic parameters, identify potential inhibitors, discriminate cancer from normal cells, and quantify glycosylases activities in various cancer cells at single-cell level, with promising potentials in biomedical and clinical applications. • We construct bidirectional strand displacement-driven 3D DNA walkers. • This nanosensor enables single-molecule monitoring of multiple human glycosylases. • This nanosensor can measure kinetic parameters and screen potential inhibitors. • This nanosensor can discriminate cancer cells from normal cells. [ABSTRACT FROM AUTHOR]
- Published
- 2023
- Full Text
- View/download PDF
13. Bsu polymerase-mediated fluorescence coding for rapid and sensitive detection of 8-oxo-7,8-dihydroguanine in telomeres of cancer cells.
- Author
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Li, Panyue, Wang, Zi-yue, Yueying Li, Liu, Ling-zhi, Qiu, Jian-Ge, and Zhang, Chun-yang
- Subjects
- *
CANCER cells , *FLUORESCENCE , *TELOMERES , *HELA cells , *CELLULAR aging , *GUANINE , *DETECTION limit , *NUCLEIC acids - Abstract
Guanine is the most susceptible to oxidation among all the DNA bases, and 8-oxo-7,8-dihydroguanine (OG) is one of main oxidation products that can occur in any part of chromosomal DNA. OG in the telomere sequence is associated with telomere shortening, cell aging, and dysfunction, and it may induce cancers. The accurate detection of OG in telomeres is important to early clinical diagnosis and molecular research. Herein, we develop a simple and rapid method to sensitively measure 8-oxo-7,8-dihydroguanine (OG) in telomeres of cancer cells by using Bsu polymerase-mediated fluorescence coding. This method is very simple without the requirement for any nucleic acid amplification or specific restriction enzyme recognition reaction, and Bsu polymerase can selectively incorporate Cy5-dATP into the opposite site of OG, endowing this method with good specificity. Moreover, the introduction of single-molecule detection significantly improves the sensitivity. This method can detect OG within 70 min with a limit of detection (LOD) of 2.45 × 10−18 M, and it can detect OG in genomic DNA extracted from H 2 O 2 -treated HeLa cells with a LOD of 0.0094 ng, holding great potential in disease-specific gene damage research and early clinic diagnosis. We develop a simple and rapid method to sensitively measure 8-oxo-7,8-dihydroguanine (OG) in telomeres of cancer cells by using Bsu polymerase-mediated fluorescence coding. [Display omitted] • We develop a simple and rapid method to sensitively measure 8-oxo-7,8-dihydroguanine (OG) in telomeres of cancer cells. • This method is on the basis of Bsu polymerase-mediated fluorescence coding. • This method exhibits high sensitivity and good specificity. • This method can detect OG within 70 min. • This method can detect OG in genomic DNA extracted from H 2 O 2 -treated HeLa cells. [ABSTRACT FROM AUTHOR]
- Published
- 2022
- Full Text
- View/download PDF
14. Gold nanoclusters enhanced electrochemiluminescence of g-C3N4 for protein kinase activity analysis and inhibition.
- Author
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Luo, Qiu-Xia, Li, Ying, Liang, Ru-Ping, Cao, Shu-Ping, Jin, Hua-Jiang, and Qiu, Jian-Ding
- Subjects
- *
PROTEIN kinases , *ELECTROCHEMILUMINESCENCE , *ELECTROLUMINESCENT polymers , *NITRIDES , *CYCLIC-AMP-dependent protein kinase , *GLUCOSE oxidase , *DETECTION limit - Abstract
In this work, a sensitive electrochemiluminescence (ECL) biosensor was proposed to detect protein kinase activity utilizing the signal amplification of bull serum albumin-templated gold nanoclusters (BSA-Au NCs) for graphite-like carbon nitride material (g-C 3 N 4). In this system, g-C 3 N 4 was utilized as a cathodic ECL emitter, and BSA-AuNCs were anchored to the phosphorylated peptides modified g-C 3 N 4 /GCE by Au S bond in the presence of adenosine 5′-[γ-thio] triphosphate (ATP-s) and protein kinase A (PKA). Attributed to the outstanding catalytic activity of BSA-AuNCs in the ECL process, the ECL intensity of this system shows 4.5-fold enhancement than that without BSA-AuNCs. Such enhancement in combination with the intensity of ECL signal and protein kinase activity enables us to fabricate an ultrasensitive ECL biosensor to detect PKA with low background. The proposed ECL platform can be utilized to analyse PKA activity of biological sample quantitatively and screen kinase inhibition qualitatively. Unlabelled Image • ECL biosensor based on the interaction between g-C 3 N 4 and Au NCs has been designed. • Au NCs can act as a catalyst to efficiently magnify the ECL intensity of g-C 3 N 4. • It provided a highly sensitive strategy for PKA activity detecting with a low detection limit. • The method proved excellent performance on the kinase inhibitor assay. [ABSTRACT FROM AUTHOR]
- Published
- 2020
- Full Text
- View/download PDF
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