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Dephosphorylation-directed tricyclic DNA amplification cascades for sensitive detection of protein tyrosine phosphatase.

Authors :
Li, Yueying
Sun, Shuli
Tian, Xiaorui
Qiu, Jian-Ge
Jiang, BingHua
Wang, Li-juan
Zhang, Chun-yang
Source :
Chemical Communications; 10/7/2020, Vol. 56 Issue 78, p11581-11584, 4p
Publication Year :
2020

Abstract

We develop a new fluorescence method for the sensitive detection of protein tyrosine phosphatase 1B (PTP1B) based on dephosphorylation-directed tricyclic DNA amplification cascades. This method exhibits good specificity and high sensitivity with a detection limit of 0.24 pM. Moreover, it can be applied for kinetics analysis, inhibitor screening, and the accurate detection of PTP1B in a variety of cancer cells. [ABSTRACT FROM AUTHOR]

Details

Language :
English
ISSN :
13597345
Volume :
56
Issue :
78
Database :
Complementary Index
Journal :
Chemical Communications
Publication Type :
Academic Journal
Accession number :
146188817
Full Text :
https://doi.org/10.1039/d0cc04714g