1. Two triterpenoid glycosides from the roots of Camellia oleifera and their cytotoxic activity.
- Author
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Yang, Ping, Li, Xia, Liu, Yan-Li, Xu, Qiong-Ming, Li, Yun-Qiu, and Yang, Shi-Lin
- Subjects
ANIMAL experimentation ,ANTINEOPLASTIC agents ,BIOLOGICAL assay ,CELL culture ,CELL lines ,CHROMATOGRAPHIC analysis ,GLYCOSIDES ,INFRARED spectroscopy ,MASS spectrometry ,MICE ,NUCLEAR magnetic resonance spectroscopy ,RESEARCH funding ,PLANT roots ,TERPENES ,TOXICITY testing ,DESCRIPTIVE statistics ,IN vitro studies - Abstract
Two new triterpenoid saponins, oleiferoside N and oleiferoside O, were isolated from the EtOH extract from the roots ofCamellia oleiferaC. Abel. Their structures were elucidated as 16α-acetoxy-21β,22α-O-diangeloyloxy-23,28-dihydroxyolean-12-ene 3β-O-β-d-xylopyranosyl-(1 → 3)-α-l-arabinopyranosyl-(1 → 3)-β-d-glucuronopyranoside (1), 16α-acetoxy-21β-O-angeloyloxy-23,28-dihydroxy-22α-O-(2-methylbutanoyloxy)olean-12-ene 3β-O-β-d-xylopyranosyl-(1 → 3)-α-l-arabinopyranosyl-(1 → 3)-β-d-glucuronopyranoside (2), on the basis of spectroscopic analyses (IR, ESI-MS, HR-ESI-MS, 1D and 2D NMR) and acid hydrolysis. Both were characterized to be oleanane-type saponins with sugar moieties linked to C-3 of the aglycone. Cytotoxic activities of two saponins were evaluated against four human tumor cell lines (A549, B16, BEL-7402, and MCF-7) by using the MTTin vitroassay. Compounds1and2showed moderate cytotoxic activities toward the tested cell lines. [ABSTRACT FROM PUBLISHER]
- Published
- 2015
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