1. Purification and characterization of 2S albumin from Nelumbo nucifera.
- Author
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Khan, Sanaullah, Ali, Syed Abid, Yasmin, Tayyaba, Ahmed, Mushtaq, and Khan, Hidayatullah
- Subjects
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PLANT 2S albumins , *EAST Indian lotus , *SEED storage compounds (Biochemistry) - Abstract
The 2S albumins are a group of seed storage proteins that have recently attracted considerable attention in the field of allergen science due to their allergenic potential. A new 2S albumin from seeds ofNelumbo nucifera(Nn-2S alb) was purified to electrophoretic homogeneity by the combination of ammonium sulfate fractionation, gel filtration, and ion exchange chromatography. The protein has a molecular mass of about 12 kDa estimated by SDS–PAGE, in good agreement with 12.5 ± 0.01 kDa determined by ESI–MS. Circular dichroism data showed that protein contained about 66% α-helices as estimated by K2D3, indicating that the protein was predominantly helical. The sedimentation coefficient (s°20,w) of the predicted model was 1.72 ± 0.21 S. The predicted 3-dimensional structure of the Nn-2S alb revealed that the protein has a region of 12 amino acids which largely corresponds to the conserved immuno-dominant epitope of 2S allergens. Surface and ribbon representations of Nn-2S albumin model, showing hydrophobic cavity in gray (A), and hypervariable region (yellow in B) and the amino acids residues (sticks) that constitute hydrophobic cavity (B). [ABSTRACT FROM PUBLISHER]
- Published
- 2016
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