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Purification and characterization of 2S albumin from Nelumbo nucifera.
- Source :
-
Bioscience, Biotechnology & Biochemistry . Nov2016, Vol. 80 Issue 11, p2109-2114. 6p. - Publication Year :
- 2016
-
Abstract
- The 2S albumins are a group of seed storage proteins that have recently attracted considerable attention in the field of allergen science due to their allergenic potential. A new 2S albumin from seeds ofNelumbo nucifera(Nn-2S alb) was purified to electrophoretic homogeneity by the combination of ammonium sulfate fractionation, gel filtration, and ion exchange chromatography. The protein has a molecular mass of about 12 kDa estimated by SDS–PAGE, in good agreement with 12.5 ± 0.01 kDa determined by ESI–MS. Circular dichroism data showed that protein contained about 66% α-helices as estimated by K2D3, indicating that the protein was predominantly helical. The sedimentation coefficient (s°20,w) of the predicted model was 1.72 ± 0.21 S. The predicted 3-dimensional structure of the Nn-2S alb revealed that the protein has a region of 12 amino acids which largely corresponds to the conserved immuno-dominant epitope of 2S allergens. Surface and ribbon representations of Nn-2S albumin model, showing hydrophobic cavity in gray (A), and hypervariable region (yellow in B) and the amino acids residues (sticks) that constitute hydrophobic cavity (B). [ABSTRACT FROM PUBLISHER]
- Subjects :
- *PLANT 2S albumins
*EAST Indian lotus
*SEED storage compounds (Biochemistry)
Subjects
Details
- Language :
- English
- ISSN :
- 09168451
- Volume :
- 80
- Issue :
- 11
- Database :
- Academic Search Index
- Journal :
- Bioscience, Biotechnology & Biochemistry
- Publication Type :
- Academic Journal
- Accession number :
- 118355590
- Full Text :
- https://doi.org/10.1080/09168451.2016.1158627