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A recent study conducted by Merck & Co. Inc. in Rahway, New Jersey, has developed a cell-based reporter potency assay for live virus vaccines. The traditional methods used to measure the potency of these vaccines are time-consuming and labor-intensive. The new assay uses a Vero E6 cell line engineered to express luciferase, which allows for a more efficient and accurate measurement of viral infection intensity. The study concludes that this reporter assay could be used to support the manufacturing and release of multiple live virus vaccines. [Extracted from the article]

A recent study conducted by researchers at Jamia Hamdard in New Delhi, India, aimed to assess serological markers for disease severity in pediatric patients with dengue infection. The study found that antibody response against immature virus was significantly higher in patients with severe disease compared to those with mild disease. Additionally, antibody titers against recombinant envelop protein were higher in patients with mild disease. The researchers concluded that antibodies targeting immature virus could be used as a predictor of disease severity and could help optimize medical care and resources. The study was published in the Journal of Medical Virology. [Extracted from the article]

A recent preprint abstract discusses the development of a new method called micro-plaque assays for detecting, isolating, and characterizing bacteriophages. The traditional plaque assay, which has been used for over 100 years, has limitations in scalability. The researchers used a robotic pinning platform to miniaturize the assay, increasing throughput by over 1000 fold without compromising sensitivity. They demonstrated the effectiveness of the method by isolating and characterizing 21 unique Pseudomonas aeruginosa phages from a single environmental sample and testing their ability to infect multi-drug resistant clinical P. aeruginosa strains. This research has not yet undergone peer review. [Extracted from the article]

Researchers at the Evandro Chagas Institute in Brazil have conducted a study on the encephalitis virus. The study aimed to investigate the potential interference between the insect-specific virus Brejeira (BREV) and the Saint Louis encephalitis virus (SLEV). The results showed that BREV had a more pronounced cytopathic effect than SLEV, and SLEV replication was suppressed in the presence of BREV. However, SLEV did not negatively interfere with BREV replication. This research provides insights into the interactions between different viruses and their potential impact on encephalitis. [Extracted from the article]

A preprint abstract from biorxiv.org discusses the use of lytic bacteriophages to treat antimicrobial-resistant bacterial infections. The study examines the role of neutrophils, the most abundant phagocytes in the body, in the pharmacokinetics of intravenously administered bacteriophages in uninfected mice. The researchers found that neutrophils have minimal impact on phage clearance, suggesting that neutrophil-mediated phagocytosis is not a major determinant of phage clearance. However, they observed substantial functional inactivation of circulating phages over time, indicating that circulating factors, rather than neutrophils, are responsible for inactivating intravenously administered phages. This research has not yet undergone peer review. [Extracted from the article]

A study conducted by Mayo Clinic in Rochester, Minnesota, compared different methods of phage susceptibility testing (PST) for Pseudomonas aeruginosa, a Gram-negative bacteria. The researchers found that there is currently no standardized approach for assessing bacterial susceptibility to phage. The study concluded that further development is needed to improve the reproducibility of PST methods. This research was supported by the National Institute of Allergy and Infectious Diseases and the Peer Reviewed Medical Research Program. [Extracted from the article]

Keywords: Fort Collins; State:Colorado; United States; North and Central America; Arbovirus Infections; Bunyaviridae Infections; Health and Medicine; Microbiological Techniques; Mosquito-Borne Diseases; Mosquitoes; Plaque Assay; RNA Virus Infections; RNA Viruses; Rift Valley Fever; Viral Hemorrhagic Fevers; Virus Cultivation EN Fort Collins State:Colorado United States North and Central America Arbovirus Infections Bunyaviridae Infections Health and Medicine Microbiological Techniques Mosquito-Borne Diseases Mosquitoes Plaque Assay RNA Virus Infections RNA Viruses Rift Valley Fever Viral Hemorrhagic Fevers Virus Cultivation 10 10 1 10/30/23 20231103 NES 231103 2023 OCT 31 (NewsRx) -- By a News Reporter-Staff News Editor at Zika & Mosquito Week -- Researchers detail new data in Mosquito-Borne Diseases - Rift Valley Fever. Fort Collins, State:Colorado, United States, North and Central America, Arbovirus Infections, Bunyaviridae Infections, Health and Medicine, Microbiological Techniques, Mosquito-Borne Diseases, Mosquitoes, Plaque Assay, RNA Virus Infections, RNA Viruses, Rift Valley Fever, Viral Hemorrhagic Fevers, Virus Cultivation. [Extracted from the article]

Reports that police officers in Denmark has launched a search on October 19, 1993 for 75 vials containing HIV cultures stolen from a hospital in Copenhagen.