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A vaginal hysterectomy can be successfully done even if a woman has a larger than normal uterus, according to a study of 119 women. Traditionally, a hysterectomy is done by making an incision in the abdomen, but the uterus can also be withdrawn through the vagina. A vaginal hysterectomy is a shorter operation, has fewer complications, and shorter hospital stays.

We report on the direct experimental observation of the 7pP2_3/2→7dD2 optical transitions in 209 and 210 francium isotopes. By continuously monitoring the fluorescence emitted by the isotopes collected in a magneto-optical trap (MOT), the electric dipole transitions 7pP2_3/2→7dD2_5/2 of Fr209, not yet experimentally observed, and 7pP2_3/2→7dD2_5/2, 7pP2_3/2→7dD2_5/2 of Fr210 were detected as sub-Doppler depletion dips of the cold atom population. This approach allowed unambiguous identification of the excited state hyperfine structures, even in the absence of a large stable vapor. Our findings demonstrate the effectiveness and the flexibility of fluorescence monitoring of trap depletion upon laser excitation, and broaden the experimental knowledge of francium isotopes and their electronic and nuclear properties. These results will have a relevant impact on ongoing researches for low-energy testing of fundamental symmetries with francium, from atomic parity non-conservation to the electron dipole moment.

Les maladies inflammatoires chroniques de l'intestin (MICI) regroupent la maladie de Crohn et la rectocolite hémorragique. En France on estime que plus de 200 000 personnes sont atteintes d'une MICI. Ces maladies incurables, touchent principalement le sujet jeune et se caractérisent par des phases de rémission auxquelles succèdent des phases de poussée. L'objectif thérapeutique actuel est une rémission clinique et une cicatrisation de la muqueuse intestinale. A notre connaissance, il n'existe pas de questionnaire validé permettant de mesurer le phénomène de la poussée dans les MICI. Les messages échangés entre patients sur les forums internet constituent un matériel brut qui émerge spontanément en dehors de toute situation de recherche. L'objectif du projet FLARE-IBD est de développer une approche innovante d'exploitation de ces messages afin de générer des items candidats de questionnaires.

Acrodermatitis enteropathic (AE) is a rare autosomal recessive disorder due to a zinc deficiency and characterized by a classical triad of symptoms: dermatitis, alopecia, and diarrhea. The defective gene is SLC39A4, which encodes a zinc transporter. Nevertheless many abnormalities in SLC39A4 have been relieved, only 50% of patients show alterations. Here is reported the case of an infant with mild and incomplete manifestations of AE, for whom the SLC39A4 genetic test was performed. A novel mutation in SLC39A4 was identified. Zinc replacement improved rapidly the skin lesions. Our case highlights the importance of suspecting this rare condition and to perform the genetic test even in those patients who do not fulfil the classical triad of symptoms. Further efforts should be addressed to identify a more strength correlation between genotype and phenotype of this disorder.

Prosthetic rehabilitation improves the patient's quality of life and oral health. The purpose of the present study was to assess the production of volatile sulfur compounds (VSCs) using Oral Chroma™ in patients wearing provisional and permanent fixed prosthesis, who were treated or not, with supportive non-surgical periodontal therapy. A total of 10 healthy patients not affected by periodontal disease and who needed the restoration of at least two edentulous single sites were included in the present study. Registrations of VSCs were carried out with a Gas Chromatograph OralChroma™ (Oral Chroma™, Abimedical, Abilit Corp., Osaka, Japan) one month after placement of the provisional restoration (group 1) and one month after placement of the final restoration (group 3). After each measurement, professional oral hygiene was carried out both on patients with provisional (group 2) and permanent prostheses (group 4) and VSC values were registered. The results showed that there were no statistical significant differences in the VSC quantity between groups with temporary or permanent prostheses. Meanwhile, statistically significant differences were found in VCS values between groups before and after the professional health care session (p < 0.05). Also it was observed that dimethyl sulphide (CH3)2S was present in all the study groups. The present preliminary study suggests that OralChroma™ produce a comprehensive assessment of VSC in the clinical diagnosis of halitosis and that professional oral hygiene seems to influence VSC production. However, further clinical long-term studies with a larger sample size are necessary for a better understanding of halitosis manifestation in patients wearing provisional and permanent fixed prosthesis.

Individuare le cause dell’insuccesso di precedenti interventi di apicectomia utilizzando il microscopio operatorio e valutare il successo clinico e radiografico del ritrattamento chirurgico a un anno.

L'adolescence est une période caractérisée d'une part par un lien fort entre le niveau de bien-être et les comportements de santé, et d'autre part une réactivité émotionnelle intense. Il est donc nécessaire de disposer d'un outil de mesure adapté à l'évaluation écologique momentanée, afin de mieux saisir les variations intrajournalières du bien-être mental des adolescents, et ainsi obtenir des données précises, permettant de comprendre comment l'environnement, les comportements, et la santé, peuvent interagir.

ABSTRACTDetection of Plasmodium ovaleby use of a nested PCR assay with a novel Plasmodium ovaleprimer set was superior to detection of Plasmodium ovaleby real-time PCR assays. Nested PCR was also better at detecting P. malariae. The detection of P. ovalein many patients first admitted >2 months following their return to Italy indicated that P. ovalerelapses are common.

Some synthetic taurine analogues, namely ethanolamine-O-sulphate (EOS), N,N-dimethyltaurine (DMT), N,N,N-trimethyltaurine (TMT) and 2-aminoethylphosphonic acid (AEP) were shown to interact with rabbit brain GABAA- or GABAB-receptors, while (±)piperidine-3-sulfonic acid (PSA) inhibited the activity of rabbit brain 4-aminobutyrate transaminase. This suggests that they behave like direct/indirect GABA agonists or GABA antagonists and affect thermoregulation and gross motor behaviour (GMB) which are under GABA control. In the present study micromole (1.2–48) amounts of these compounds were i.c.v. injected in conscious, restrained rabbits while monitoring rectal temperature (RT), ear skin temperature (EST) and GMB. AEP, EOS, DMT and TMT induced a dose-related hyperthermia, ear vasoconstriction and excitation of GMB, while PSA induced a dose-related hypothermia, ear vasodilation and inhibition of GMB. EOS antagonized in a dose-related fashion hypothermia induced by 60 nmol THIP, a GABAAagonist, while AEP, DMT and TMT counteracted that induced by 8 nmol R(-)Baclofen, a GABABagonist.Some synthetic taurine analogues, namely ethanolamine-O-sulphate (EOS), N,N-dimethyltaurine (DMT), N,N,N-trimethyltaurine (TMT) and 2-aminoethylphosphonic acid (AEP) were shown to interact with rabbit brain GABAA- or GABAB-receptors, while (±)piperidine-3-sulfonic acid (PSA) inhibited the activity of rabbit brain 4-aminobutyrate transaminase. This suggests that they behave like direct/indirect GABA agonists or GABA antagonists and affect thermoregulation and gross motor behaviour (GMB) which are under GABA control. In the present study micromole (1.2–48) amounts of these compounds were i.c.v. injected in conscious, restrained rabbits while monitoring rectal temperature (RT), ear skin temperature (EST) and GMB. AEP, EOS, DMT and TMT induced a dose-related hyperthermia, ear vasoconstriction and excitation of GMB, while PSA induced a dose-related hypothermia, ear vasodilation and inhibition of GMB. EOS antagonized in a dose-related fashion hypothermia induced by 60 nmol THIP, a GABAAagonist, while AEP, DMT and TMT counteracted that induced by 8 nmol R(-)Baclofen, a GABABagonist.

We report on the characterization of the behaviour of an anisotropic magnetoresistive sensor undergoing a 100 kHz flipping of the magnetic domains, i.e. at frequencies two/three orders of magnitude higher than conventionally recommended. The noise analysis allows for the optimal setting of the relevant parameters defining such high-frequency, and hitherto unexplored, operation regime. Precision and accuracy performance in static conditions have been assessed by keeping into account the role of the sensor temperature. This last parameter has been evaluated by suitably manipulating the signals occurring at the sensor itself. The used technique can provide the base for a temperature sensor conditioning to be employed in magnetometers using this kind of devices.

ABSTRACTA TaqMan-based real-time PCR qualitative assay for the detection of three species of malaria parasites—Plasmodium falciparum, P. ovale, and P. vivax—was devised and evaluated using 122 whole-blood samples from patients who had traveled to areas where malaria is endemic and who presented with malaria-like symptoms and fever. The assay was compared to conventional microscopy and to an established nested-PCR assay. The specificity of the new assay was confirmed by sequencing the PCR products from all the positive samples and by the lack of cross-reactivity with Toxoplasma gondiiand Leishmania infantumDNA. Real-time PCR assay showed a detection limit (analytical sensitivity) of 0.7, 4, and 1.5 parasites/µl for P. falciparum, P. vivax, and P. ovale, respectively. Real-time PCR, like nested PCR, brought to light errors in the species identification by microscopic examination and revealed the presence of mixed infections (P. falciparumplus P. ovale). Real-time PCR can yield results within 2 h, does not require post-PCR processing, reduces sample handling, and minimizes the risks of contamination. The assay can therefore be easily implemented in routine diagnostic malaria tests. Future studies are warranted to investigate the clinical value of this technique.

The ACE‐2 HILLCLOUD experiment was carried out on the island of Tenerife in June–July 1997 to investigate the interaction of the boundary layer aerosol with a hill cap cloud forming over a ridge to the north‐east of the island. The cloud was used as a natural flow through reactor to investigate the dependence of the cloud microphysics and chemistry on the characteristics of the aerosols and trace gases entering cloud, and to simultaneously study the influence of the physical and chemical processes occurring within the cloud on the size distribution, chemical and hygroscopic properties of the aerosol exiting cloud. 5 major ground base sites were used, measuring trace gases and aerosols upwind and downwind of the cloud, and cloud microphysics and chemistry and interstitial aerosol and gases within the cloud on the hill. 8 intensive measurement periods or runs were undertaken during cloud events, (nocturnally for seven of the eight runs) and were carried out in a wide range of airmass conditions from clean maritime to polluted continental. Polluted air was characterised by higher than average concentrations of ozone (>50 ppbv), fine and accumulation mode aerosols (>3000 and >1500 cm−3, respectively) and higher aerosol mass loadings. Cloud droplet number concentrations N, increased from 50 cm−3in background maritime air to >2500 cm−3in aged polluted continental air, a concentration much higher than had previously been detected. Surprisingly, Nwas seen to vary almost linearly with aerosol number across this range. The droplet aerosol analyser (DAA) measured higher droplet numbers than the corrected forward scattering spectrometer probe (FSSP) in the most polluted air, but at other times there was good agreement (FSSP=0.95 DAA with an r2=0.89 for N<1200 cm−3). Background ammonia gas concentrations were around 0.3 ppbv even in air originating over the ocean, another unexpected but important result for the region. NO2was present in background concentrations of typically 15 pptv to 100 pptv and NO˙3 (the nitrate radical) was observed at night throughout. Calculations suggest NO˙3 losses were mainly by reaction with DMS to produce nitric acid. Low concentrations of SO2(∼30 pptv), HNO3and HCl were always present. HNO3concentrations were higher in polluted episodes and calculations implied that these exceeded those which could be accounted for by NO2oxidation. It is presumed that nitric and hydrochloric acids were present as a result of outgassing from aerosol, the HNO3from nitrate rich aerosol transported into the region from upwind of Tenerife, and HCl from sea salt aerosol newly formed at the sea surface. The oxidants hydrogen peroxide and ozone were abundant (i.e., were well in excess over SO2throughout the experiment). Occasions of significant aerosol growth following cloud processing were observed, particularly in cleaner cases. Observations and modelling suggested this was due mainly to the take up of nitric acid, hydrochloric acid and ammonia by the smallest activated aerosol particles. On a few occasions a small contribution was made by the in‐cloud oxidation of S(IV). The implications of these results from HILLCLOUD for the climatologically more important stratocumulus Marine Boundary Layer (MBL) clouds are considered.

Experiments with electrons and ions in a combined trap are reported. The unique capability to confine particles with opposite charge and very different mass simultaneously in the same spatial region makes the combined trap a promising device for future synthesis of antihydrogen.

Thermolabile (LT) enterotoxin was prepared from stationary and shaken cultures of enterotoxigenicEscherichia coli (ETEC) grown in casamino acids-yeast extract medium and dried onto filter discs. These were then examined by a modification of the single radial immune haemolysis (SRIH) test. It was observed that LT antigenicity, as detected by this test, remained unaltered for as long as 30 days at room temperature.

The production of thermolabile (LT) enterotoxin was compared in a defined medium reported by Staples et al. (SAG medium) for the production of thermostable (ST) enterotoxin and the Casamino acids-Yeast extract (CAYE) medium. Aliquots were drawn from cultures of an enterotoxigenic (LT+, ST+)E. coli in both media at different times, growth curves were plotted, and culture filtrates tested for toxin activity. Levels of LT and ST in the SAG showed that it is as suitable as CAYE for the production of LT.

An indirect haemagglutination test (IH) for the detection of enterotoxigenicE. coli (LT) was developed. Twenty-five enterotoxigenicE. coli (ETEC) from human and porcine diarrhoca and from river water were examined. The described IH test was more specific and sensitive than the passive immune haemolysis test (PIH).

The effect of Escherichia coli enterotoxin STa on the primary and secondary immune response in F1 (CBA × C57 B1/10) mice immunized against sheep red blood cells (SRBC) was investigated. Modulating action on the IgM and IgG response was found to be dependent on the dose-time administration of the toxin. Immunosuppression of the primary response on the 4th day after immunization was observed when the toxin was injected 15 min before the SRBC, followed by immunostimulation on the 6th day after antigen (Ag) injection. Moreover, toxin administration 48 h before SRBC caused immunosuppression of the primary immune response on the 4th and 6th days. On the other hand, the IgM and IgG secondary immune response, determined 6 days after boosting, was greatly enhanced by toxin administration 15 min before priming (day 0) or boosting (day 26) and 48 h before priming. The same response was suppressed by toxin administration 48 h before booster antigen injection.

A method that allows the calculation of magnetic fields produced by cylindrically symmetric configurations of permanent magnets and high permeability materials is presented. The method is based on a non-iterative finite-element algorithm and can be utilized on small-scale computing facilities. As an example, the design of a magnetic trap for neutral atoms is discussed. Comparisons of calculations with analytical and experimental data are also reported.

Antineoplastic compounds can induce on tumour cells new antigens that undetectable on parental cells and which are transmissible as a genetic character. In this study mouse leukaemia L1210 was cloned in vitro by limiting dilution and one cloned line was recloned in vivo. Four subcloned tumour cell lines (A,D,R,S) were xenogenized in vivo by DTIC treatment (A/DTIC, D/DTIC, R/DTIC, S/DTIC) following a schedule previously described. Up to 10(7) cells of these xenogenized subclones, injected i.p., were rejected by syngeneic hosts, although they grew in immunosuppressed hosts. The DTIC treated subclones were lysed by in vivo-primed, in vitro-restimulated (with the relevant subclone) lymphocytes. The cytotoxic lymphocyte activity was not strictly specific since parental, DTIC-untreated cells were also lysed, although less efficiently. CTL directed against the D/DTIC subclone were cloned by limiting dilution. Ninety-four CTL clones were assayed against L1210 subcloned cells, DTIC-treated and untreated, and against different murine tumours (syngeneic or allogenic). Three specific antigens could be identified in the 51Cr release assay. The DTIC subclones expressed one antigen that was specifically recognized by a set of CTL clones. A number of CTL clones were able to lyse the L1210 subcloned cell exclusively, targetting a tumour-associated antigen that did not appear to be modified in the DTIC-treated subclones. A third antigen was demonstrated in the parental and DTIC treated D subclone. On the basis of these results it was postulated that there was at least one common DTIC-inducible antigen specific and reproducible within an identical cell population. Moreover, DTIC treatment did not modify histocompatibility antigens or TAA pre-existing in L1210 cells. The findings discussed here provide new information about permanent xenogenization of tumour cells, which might be exploited for experimental chemo-immunotherapy of cancer.

Polyomavirus mutants capable of replicating in undifferentiated murine C2 myoblasts were selected and characterized. These mutants grow normally in 3T6 mouse fibroblast cells, and they do not complement the wild-type virus in coinfection experiments of C2 myoblasts. Of 12 isolates, 10 possess duplications of the regulatory region including the enhancer A domain. On the bases of the regulatory region structure and the presence and length of the enhancer duplication, the mutant viruses could be grouped into three classes. One mutant class (e.g., PyMB3) possesses an enhancer duplication of 91 bp identical to that of a previously characterized polyomavirus mutant, PyNB11/1. We have demonstrated that this enhancer duplication gives rise at its junction to a novel recognition motif for the transcriptional factor NF-1 (M. Caruso, C. Iacobini, C. Passananti, A. Felsani, and P. Amati, EMBO J. 9:947-955, 1990). The regulatory region PyMB3 virus recombined in a wild-type genome context maintains the mutant phenotype. The other two types of mutants, one with a 30-bp enhancer duplication (e.g., PyMB40) and one with a wild-type enhancer structure (e.g., PyMB27), possess two similar but distinct 6-bp deletions in the same region of the VP1 coding gene. In both cases, the ability to replicate in undifferentiated C2 myoblasts is strictly correlated to the mutation in the VP1 coding region.

Décrire les caractéristiques, les comorbidités et le traitement des patients atteints d’hypercholestérolémie familiale (HF), certaine ou probable, chez des patients suivis en ambulatoire.

Tunable laser radiation with megahertz linewidth is generated with a simple, inexpensive, and compact laser system that uses two common microscope slides as the only intracavity tuning elements. The laser emits two radiation modes whose frequencies are separated by 1.2 GHz, corresponding to the free spectral range of the laser resonator. The frequencies may be rapidly varied over a range of 1.5 GHz at a rate of 2 GHz/s.

The circadian patterns in the onset of symptoms of acute myocardial infarction were assessed in a prospective study involving 450 patients. Analysis of the data in the 424 patients in whom the time of onset was known did not show any statistically significant circadian rhythms, either for the group as a whole or for subgroups divided according to sex, age group (< 60, 60 − 69, 70 − 79 and ≥ 80 years old), or the presence or absence of diabetes mellitus. The frequency of onset of symptoms was highest in the morning (06.01 − 12.00 h) for the group as a whole (29.2%). Patients aged < 70 and ≥ 80 years old showed a peak in the morning, whereas patients aged 70 − 79 years old showed a peak in the afternoon (12.01 − 18.00 h).

Aims.We study the distribution of stellar ages in the Orion nebula cluster (ONC) using accurate HST photometry taken from HST Treasury Program observations of the ONC utilizing the cluster distance estimated by Menten and collaborators. We investigate whether there is an intrinsic age spread in the region and whether the age depends on the spatial distribution.

We present the sub-mm/mm SED for a sample of eight young circumstellar disks in the outer regions of the Orion nebula cluster (ONC). New observations were carried out at 2.9 mm with the CARMA array and for one disk, 216-0939, at 3.3 and 6.8 mm with ATCA. By combining these new millimeter data with literature measurements at sub-millimeter wavelengths we investigate grain growth and measure the dust mass in protoplanetary disks in the ONC. These data provide evidence for dust grain growth to at least millimeter-sizes for the first time in a high-mass star-forming region. The obtained range in sub-mm/mm spectral index, namely 1.5–3.2, indicates that for disks in the outskirts of the ONC (projected distance from the cluster center between about 0.4 pc and 1.5 pc) grain growth to mm sizes occurs in the same manner as disks in regions where only low-mass stars form. Finally, in our sample three disks are more massive than about 0.05 M⊙, confirming that massive disks are present in the outer regions of the Orion nebula.

We present new ATCA observations at 3.3 mm of 27 young stellar objects in the ρ-Oph young cluster. 25 of these sources have been detected. We analyze the sub-millimeter and millimeter SED for a subsample of 17 isolated class II protoplanetary disks and derive constraints on the grain growth and total dust mass in the disk outer regions. All the disks in our sample show a mm slope of the SED which is significantly shallower than the one observed for the ISM at these long wavelengths. This indicates that 1) class II disks in Ophiuchus host grains grown to mm/cm-sizes in their outer regions; 2) formation of mm/cm-sized pebbles is a fast process and 3) a mechanism halting or slowing down the inward radial drift of solid particles is required to explain the data. These findings are consistent with previous results in other star forming regions. We compare the dust properties of this sample with those of a uniformly selected sample in Taurus-Auriga and find no statistical evidence of any difference in terms of grain growth between the two regions. Finally, in our sample the mm slope of the SED is not found to correlate with indicators of grain growth to micron sizes in the surface layers of the inner disk.

Context. Observations at sub-millimeter and mm wavelengths will in the near future be able to resolve the radial dependence of the mm spectral slope in circumstellar disks with a resolution of around a few AU at the distance of the closest star-forming regions.Aims. We aim to constrain physical models of grain growth and fragmentation by a large sample of (sub-)mm observations of disks around pre-main sequence stars in the Taurus-Auriga and Ophiuchus star-forming regions.Methods. State-of-the-art coagulation/fragmentation and disk-structure codes are coupled to produce steady-state grain size distributions and to predict the spectral slopes at (sub-)mm wavelengths.Results. This work presents the first calculations predicting the mm spectral slope based on a physical model of grain growth. Our models can quite naturally reproduce the observed mm-slopes, but a simultaneous match to the observed range of flux levels can only be reached by a reduction of the dust mass by a factor of a few up to about 30 while keeping the gas mass of the disk the same. This dust reduction can either be caused by radial drift at a reduced rate or during an earlier evolutionary time (otherwise the predicted fluxes would become too low) or due to efficient conversion of dust into larger, unseen bodies.

We present the most sensitive 3 mm-survey to date of protoplanetary disks carried out in the Taurus-Auriga star forming region (average rms of about 0.3 mJy), using the IRAM PdBI. With our high detection rate of 17/19 we provide the first detections at wavelengths longer than about 1 mm for 12 sources. This enables us to statistically study the mm SED slopes and dust properties of faint disks and compare them to brighter disks using a uniform analysis method. With these new data and literature measurements at sub-millimeter and millimeter wavelengths, we analyze the dust properties of a sample of 21 isolated disks around T Tauri stars in the Taurus-Auriga star forming region. Together with the information about the disks' spatial extension from sub/mm-mm interferometric studies, we derive from the observed sub-mm/mm spectral energy distribution constraints on the dust opacity law at these wavelengths, using two-layer flared disk models and a self-consistent dust model that properly takes into account the variation of the dust opacity with grain growth. We find evidence for the presence of dust particles in the disk midplane that have grown to sizes as large as at least 1 millimeter in all the disks of our sample, confirming what was previously observed on smaller brighter objects. This indicates that the dust coagulation from ISM dust to mm-sized grains is a very fast process in protoplanetary disks, which appears to occur before a young stellar object enters the class II evolutionary stage. The amount of these large grains in the disk outer regions is also found to be stationary throughout the whole class II evolutionary stage, which indicates that mechanisms slowing down the dust inward migration play an important role in the Taurus-Auriga protoplanetary disks. Another result is that the spectral index between 1 and 3 mm for the six faintest disks in our sample is on average smaller than for the brighter disks, indicating either that these fainter yet unmapped disks are spatially much less extended than the brighter spatially resolved disks, or that fainter disks have typically larger dust grains in their outer regions. Considering that these fainter disks are more representative of the bulk of the disk population than the brighter ones, this may have important consequences for the theories of planetesimal formation and disk formation and evolution. Finally, we investigate the relations between the derived dust properties, namely dust mass and grain growth, and the properties of the central star, like its mass, age and mass accretion rate.

&lt;it>Objective&lt;/it>. Kawasaki disease (KD) is an acute febrile systemic vasculitis, mainly affecting infants and young children. Immunological abnormalities during the acute phase of KD have been described extensively. However, the occurrence of a second immunological disorder in a patient with a history of KD is rarely reported. We evaluated the presence of autoimmune thyroiditis and coeliac disease (CD) in patients with KD diagnosis. &lt;it>Methods&lt;/it>. Ninety consecutive children (57 males and 33 females, median age 5.2 yr, age range 1.6–14.1 yr) with KD were evaluated. All patients were evaluated for thyroid function (thyroid-stimulating hormone, thyroxine and triiodothyronine), anti-thyroglobulin (TgA) and anti-peroxidase (TPOA) antibodies, and antigliadin, anti-endomysium and antitransglutaminase antibodies. CD was confirmed by jejunal biopsy if the specific antibody profile was positive. One hundred and fifty Italian children, matched for age and sex and from the same geographic area, acted as controls. &lt;it>Results&lt;/it>. A total of five patients (three boys, two girls; 5.5&percnt;; &lt;it>P&lt;/it>&lt;0.05) were found positive for coeliac antibodies. In all of these patients the diagnosis of CD was confirmed histologically. Regarding thyroid function and autoantibodies, no patient showed subclinical hypothyroidism or autoimmune thyroiditis. No differences in the familial occurrence of autoimmune diseases between KD patients and controls were found (9.1 and 7.9&percnt;, respectively). &lt;it>Conclusions&lt;/it>. Our data showed a higher prevalence of CD in children with KD, and this suggests that children with KD should be monitored carefully for CD. However, there was no increase in the prevalence of autoimmune thyroid diseases in patients with KD or the familial occurrence of autoimmune diseases.

Paracoccidioides brasiliensis probably uses many different mechanisms to establish itself in the host and cause disease. In this work, we assess an in vitro model system which uses cultured mammalian cells to investigate the virulence factors of P. brasiliensis. We were able to demonstrate an invasion process of the yeast form of this fungus in Vero cell cultures. We deduced that the overall invasive process involved three steps: adhesion, followed by invasion of individual epithelial cells and spread to adjacent cells.