1. Potential of utilizing almond hull extract for filamentous fungi production by submerged cultivation
- Author
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Cao, Lin, Barzee, Tyler J., El Mashad, Hamed M., Pan, Zhongli, and Zhang, Ruihong
- Abstract
Almond hulls can be used as an inexpensive source of nutrients for producing fungi that can be used as nontraditional foods such as alternative protein, probiotics, and food ingredients. Nutrients were extracted from almond hulls using hot water and their utility in cultivating filamentous fungus Aspergillus awamori(A. awamori) in submerged cultivation was investigated using batch fermentation in flasks with passive aeration. The almond hulls extract supplied all the nutrients required for A. awamorigrowth. The uptake preference of various sugars was investigated and the growth kinetic parameters of A. awamoriwere determined. Utilization of sugars by A. awamorigrown in almond hull extract proceeded sequentially with preference for glucose followed by sucrose, fructose, and then xylose. Compared to potato dextrose broth medium, the fungal biomass produced using almond hull extract as growth medium had higher protein (19.59%) and lower fat (1.50%) contents. The modified Gompertz model described the kinetics of A. awamoriwell with a lag phase of 0.18 days and a specific growth rate of 1.77 d−1. This study explored the potential of almond hulls used as a cheap substrate for edible filamentous fungal biomass production. Sugars and other nutrients were extracted from almond hulls by hot water. Aspergillus awamoriwas able to utilize all the four types of sugar in almond hull extract. The modified Gompertze model was found to fit the fungal growth kinetics well. Fungi cultivated in almond hull extract had higher protein content and lower fat content than those cultivated from a commercial medium.
- Published
- 2024
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