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This study delved into the relationship between umami taste sensitivity (UTS) and variations in the salivary proteome among 12 healthy nonsmokers utilizing 4D data-independent acquisition-based proteomics. By assessing UTS through monosodium l-glutamate (MSG) detection thresholds, we discovered notable differences: individuals with high UTS detected umami at significantly lower MSG concentrations (0.20 ± 0.12 mM) compared to their low UTS counterparts (2.51 ± 1.21 mM). Both groups showed an upregulation of the S100A1 protein under MSG stimulation, indicating a potent biochemical response to umami stimuli. The high UTS group exhibited enhanced metabolic pathways including those for amino acid, lipid, and organic acid biosynthesis, essential for maintaining taste receptor functionality and enhancing signal transduction. This group also demonstrated increased activity in cytochrome P450 enzymes and ribonucleoprotein complexes, suggesting a readiness to manage metabolic challenges and optimize umami perception. In contrast, the low UTS group showed adaptive mechanisms, possibly through modulation of receptor availability and function, with an upregulation of structural and ribosomal proteins that may support taste receptor production and turnover. These findings suggest that varying biological mechanisms underpin differences in umami perception, which could significantly influence dietary preferences and nutritional outcomes, highlighting the intricate interplay of genetic, physiological, and metabolic factors in taste sensitivity.

Quantitative glycosylation analysis serves as an effective tool for detecting changes in glycosylation patterns in cancer and various diseases. However, compared with N-glycans, O-glycans present challenges in both qualitative and quantitative mass spectrometry analysis due to their low abundance, ease of peeling, lack of a universal enzyme, and difficult accessibility. To address this challenge, we developed O-GlycoIsoQuant, a novel O-glycome quantitative approach utilizing superbase release and isotopic Girard’s P labeling. This method facilitates rapid and efficient nonreducing ß-elimination to dissociate O-glycans from proteins using the organic superbase, 1,8-diazabicyclo[5.4.0]undec-7-ene (DBU), combined with light and heavy isotopic Girard’s reagent P (GP) labeling for relative quantification of O-glycans by mass spectrometry. Employing this method, labeled O-glycans exhibit a double peak with a mass difference of 5 Da, suitable for stable relative quantification. The O-GlycoIsoQuant method is characterized by its high labeling efficiency, excellent reproducibility (CV < 20%), and good linearity (R2> 0.99), across a dynamic range spanning a 100-fold range. This method was applied to various complex sample types, including human serum, porcine spermatozoa, human saliva, and urinary extracellular vesicles, detecting 33, 39, 49, and 37 O-glycans, respectively, thereby demonstrating its broad applicability.

Phosphorus is a promising anode material for high-rate lithium ion batteries (LIBs) due to its superior theoretical specific capacity (2596 mAh g−1) and suitable lithiation potential. However, the phosphorus nanoparticles usually have poor electrical conductivity and undergo volume expansion during lithiation. Herein, a coaxial bilayer bottlebrush-like carbon@fibrous‑phosphorus@carbon-nanotube (C@FP@CNT) composite is synthesized by a chemical vapor transport method and in situ carbonization for fibrous phosphorus deposition and surface carbon deposition, respectively. The phosphorus mass loading of C@FP@CNT is as high as 71 %, and all the FP nanorods adhere tightly to the conductive CNTs and surface coating composed of amorphous carbon. FP promotes the carbonization of polyimide (PI) and improves the electrical conductivity of the PI-originated carbon coating. Owing to the unique bilayer coaxial nanostructure, the C@FP@CNT anode shows high capacities of 1852.9 and 1108.1 mAh g−1at 0.1 and 4.0 A g−1, a capacity of 1451.4 mAh g−1at 0.2 A g−1even after 300 cycles, as well as a capacity retention rate of 84.4 %. Meanwhile, the full cells comprising C@FP@CNT anode and LiCoO2cathode also displays a highly stable cycling performance and a high specific energy density. The results reveal a viable strategy to prepare high-performance alloy-type anodes for LIBs.

Hexanal and (E)-2-hexenal in soymilk mainly form during the soaking and grinding of soybeans. In this study, freshly dehulled soybeans were soaked or ground in the presence or absence of different enzyme inhibitors. The results showed that (1) 1-palmitoyl-2-linoleoyl-sn-3-phosphatidylcholine, 1-stearoyl-2-linoleoyl-sn-3-phosphatidylcholine, 1-palmitoyl-2-linolenoyl-sn-3-phosphatidylcholine, and 1-stearoyl-2-linolenoyl-sn-3-phosphatidylcholine were preferentially acted upon by lipoxygenases (LOXs) and made predominant contributions to hexanal/(E)-2-hexenal formation. Phospholipase A2(PLA2) is one of the key enzymes for hexanal/(E)-2-hexenal formation. (2) The ratio of net increase in hexanal/(E)-2-hexenal and net decrease in linoleic acid/linolenic acid was close to 100% during soaking, but it was only 60% during grinding. Only 13-hydroperoxy octadecad(tr)ienoic acid (13-HPOD/T) was formed for the membrane LOX, but both 13- and 9-hydroperoxy octadecad(tr)ienoic acid (9-HPOD/T) were produced for the cytoplasm LOX. Thus, only the membrane LOX was involved during soaking, while both membrane- and cytoplasm-bound LOXs worked during grinding. (3) Hydroperoxides and hexanal/(E)-2-hexenal during soybean grinding were studied. PC hydroperoxides formed almost instantly and reached a maximum in 10 s, while fatty acid hydroperoxides and hexanal/(E)-2-hexenal formed relatively slowly and reached a maximum in 50 s. The experimental data were fitted to the integrated form of the Michaelis–Menten equation, and Km, Vmax, and kcatfor the LOX, PLA2, and hydroperoxide lyase were obtained, respectively.

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Flavor is an essential quality characteristic of soymilk. (E)-2-Heptenal has a fatty and fruity flavor with the sensory threshold value of 13 μg/L in water. This study demonstrated that the formation of (E)-2-heptenal was independent of the lipoxygenase (LOX) and hydroperoxide lyase (HPL) activity as well as oxygen concentration but was related to the presence/absence of Fe2+and chelators. In a dry matter base, soybean hypocotyls generated a much higher amount of (E)-2-heptenal than cotyledons. A phospholipid hydroperoxide was purified from the chloroform/methanol extract of soybean hypocotyls and was identified as 1-palmitoyl-2-(12-hydroperoxyoctadecadienoyl)-sn-glycerol-3-phosphatidylethanol-amine (12-PEOOH). The decomposition of 12-PEOOH in the presence of ferrous ions to form (E)-2-heptenal was studied in a model system. The rate of decomposition decreased sharply at pH values higher than 6, but the molar conversion of 12-PEOOH to (E)-2-heptenal increased with an increase of pH. At a constant pH of 5.8, the decomposition rate of 12-PEOOH was positively linearly related to the Fe2+concentration, while the molar conversion to (E)-2-heptenal was 74% and independent of the Fe2+concentration. The formation of radicals LOO•and R•showed similar pH and Fe2+concentration dependence with those of (E)-2-heptenal. (E)-2-Heptenal displayed an enhancement of bean aroma and fruity flavor of soymilk at low concentrations, but a fatty flavor was noticed at high concentrations.

ABSTRACTEndophytic fungi and bacteria are the most ubiquitous and representative commensal members that have been studied so far in various higher plants. Within colonization and interaction with their host plants, endophytic microbiota are reportedly to modulate not only the host’s growth but also holobiont resilience to abiotic and biotic stresses, providing a natural reservoir and a promising solution for sustainable agricultural development challenged by global climate change. Moreover, possessing the talent to produce a wide array of high-value natural products, plant endophytic microbiota also serve as an alternative way for novel drug discovery. In this review, tea, one of the world’s three largest nonalcoholic beverages and a worldwide economic woody crop, was highlighted in the context of endophytic microbiota. We explore the recent studies regarding isolation approaches, distribution characteristics and diversity, and also biological functions of endophytic microbiota in Camellia sinensis(L.) O. Kuntze. Profoundly, the future insight into interaction mechanism between endophytic microbiota and tea plants will shed light on in-depth exploration of tea microbial resources.

Tn antigen (CD175), recognized as the precursor monosaccharide (α-GalNAc) of mucin O-glycan, is a well-known tumor-associated carbohydrate antigen (TACA). It has emerged as a potential biomarker for cancer diagnosis and prognosis. However, the role it plays in cancer biology remains elusive due to the absence of a sensitive and selective detection method. In this study, we synthesized two new probes based on a unique uridine-5′-diphospho-α-d-galactose (UDP-Gal) derivative, each functionalized with either a fluorescence or a cleavable biotin tag, to develop an innovative one-step enzymatic labeling strategy, enabling the visualization, enrichment, and site-specific mapping of the Tn antigen with unparalleled sensitivity and specificity. Our versatile strategy has been successfully applied to detect and image Tn antigen across various samples, including the complex cell lysates, live cells, serum, and tissue samples. Compared to the traditional lectin method, this one-step enzymatic method is simpler and more efficient (>10/100-fold in sensitivity). Furthermore, it allowed us to map 454 Tn-glycoproteins and 624 Tn-glycosylation sites from HEK293FTn+and Jurkat cells. Therefore, our strategy provides an exceptionally promising tool for revealing the biological functions of the Tn antigen and advancing cancer diagnostics.

Wuyi Rock tea, specifically Shuixian and Rougui, exhibits distinct sensory characteristics. In this study, we investigated the sensory and metabolite differences between Shuixian and Rougui. Quantitative description analysis revealed that Rougui exhibited higher intensity in bitter, thick, harsh, and numb tastes, while Shuixian had stronger salty and umami tastes. Nontargeted metabolomics identified 151 compounds with 66 compounds identified as key differential metabolites responsible for metabolic discrimination. Most of the catechins and flavonoids were enriched in Rougui tea, while epigallocatechin-3,3′-di-O-gallate, epigallocatechin-3,5-di-O-gallate, gallocatechin-3,5-di-O-gallate, isovitexin, and theaflavanoside I were enriched in Shuixian tea. Catechins, kaempferol, quercetin, and myricetin derivatives were positively correlated with bitter taste and numb sensation. Sour taste was positively correlated to organic acids. Amino acids potentially contributed to salty and umami tastes. These results provide further insights into the taste characteristics and the relationship between taste attributes and specific metabolites in Wuyi Rock tea.

A novel β-primeverosidase-like enzyme, originating from the hypocotyl of soybeans, was isolated and characterized. This enzyme, with an estimated molecular weight of 44 kDa, was identified as a monomer and exhibited peak activity at 55 °C and pH 5.5. It demonstrated a specific and efficient hydrolysis of 1-octen-3-yl β-primeveroside (1-octen-3-yl prim) and 3-octanyl β-primeveroside (3-octanyl prim) but did not act on glucopyranosides. Mn2+significantly enhanced its activity, while Zn2+, Cu2+, and Hg2+exerted inhibitory effects. Kinetic analysis revealed a higher hydrolytic capacity toward 1-octen-3-yl prim. Partial amino acid sequences were determined and the N-terminal amino acid sequence was determined to be AIVAYAL ALSKRAIAAQ. The binding energy and binding free energy between the β-primeverosidase enzyme and its substrates were observed to be higher than that of β-glucosidase, thus validating its superior hydrolysis efficiency. Hydrogen bonds and hydrophobic interactions were the main types of interactions between β-primeverosidase enzyme and 1-octen-3-yl prim and 3-octanyl prim, involving amino acid residues such as GLU-470, TRP-463, GLU-416, TRP-471, GLN-53, and GLN-477 (hydrogen bonds) and PHE-389, TYR-345, LEU-216, and TYR-275 (hydrophobic interactions). This study contributes to the application of a β-primeverosidase-like enzyme in improving the release efficiency of glycosidically conjugated flavor substances.

Roasting is pivotal for enhancing the flavor of Wuyi rock tea (WRT). A study investigated a novel compound that enhances the umami taste of WRT. Metabolomics of Shuixian tea (SXT) and Rougui tea (RGT) under light roasting (LR), medium roasting (MR), and heavy roasting (HR) revealed significant differences in nonvolatiles compounds. Compared LR reducing sugars and amino acids notably decreased in MR and HR, with l-alanine declining by 69%. Taste-guided fractionation identified fraction II–B as having high umami and sweet intensities. A surprising taste enhancer, N-(1-carboxyethyl)-6-(hydroxymethyl) pyridinium-3-ol (alapyridaine), was discovered and identified. It formed via the Maillard reaction, positively correlated with roasting in SXT and RGT. Alapyridaine levels were highest in SXT among the five oolong teas. Roasting tea with glucose increased alapyridaine levels, while EGCG inhibited its formation. HR-WRT exhibited enhanced umami and sweet taste, highlighting alapyridaine’s impact on WRT’s flavor profile. The formation of alapyridaine during the roasting process provides new insights into the umami and sweet perception of oolong tea.

Wuyi Rock Tea (WRT) is widely appreciated for its unique rocky flavor and floral essence. However, the characterization of taste attributes, particularly bitterness, in this premium tea remains largely underexplored. This study aims to assess the perception of bitterness in WRT through an integrated approach, including sensory evaluation, phenolic compound analysis, and cell-based calcium imaging assay. Sensory evaluation revealed distinct bitterness intensities among nine selected typical WRT samples. Utilizing UHPLC-Q-Orbitrap-MS, 54 phenolics were tentatively identified, comprising of 24 flavanols, 18 flavonols, and 12 phenolic acids. The cell-based calcium imaging assay revealed diverse intracellular calcium responses and half-maximum effective concentration (EC50) values across the tested samples. Notably, correlation analysis unveiled a strong negative correlation between sensory scores and EC50 values. Furthermore, 11 individual phenolic compounds, such as epigallocatechin gallate and epicatechin gallate, demonstrated significant correlation with sensory scores and bitter taste receptor activation, suggesting their potential role as key contributors shaping the bitter taste perception of WRT. This study provides comprehensive insights into the nuanced bitterness of WRT, bridging sensory and molecular perspectives to enhance our understanding of the taste profile of this premium tea.

Protein modification by lipid-derived electrophiles (LDEs) is associated with various signaling pathways. Among these LDEs, 4-hydroxy-2-nonenal (HNE) is the most toxic, and protein modified with HNE has been linked to various diseases, including Alzheimer’s and Parkinson’s. However, due to their low abundance, in-depth profiling of HNE modifications still presents challenges. This study introduces a novel strategy utilizing reversible thiazolidine chemistry to selectively capture HNE-modified proteins and a palladium-mediated cleavage reaction to release them. Thousands of HNE-modified sites in different cell lines were identified. Combined with ABPP, we discovered a set of HNE-sensitive sites that offer a new tool for studying LDE modifications in proteomes.

ABSTRACTPrecise identification of binary building changes through remote sensing observations plays a crucial role in sustainable urban development. However, many supervised change detection (CD) methods overly rely on labeled samples, thus limiting their generalizability. In addition, existing semi-supervised CD methods suffer from instability, complexity, and limited applicability. To overcome these challenges and fully utilize unlabeled samples, we proposed a consistency-guided lightweight semi-supervised binary change detection method (Semi-LCD). We designed a lightweight dual-branch CD network to extract image features while reducing model size and complexity. Semi-LCD fully exploits unlabeled samples by data augmentation, consistency regularization, and pseudo-labeling, thereby enhancing its detection performance and generalization capability. To validate the effectiveness and superior performance of Semi-LCD, we conducted experiments on three building CD datasets. Detection results indicate that Semi-LCD outperforms competing methods, quantitatively and qualitatively, achieving the optimal balance between performance and model size. Furthermore, ablation experiments validate the robustness and advantages of the Semi-LCD in effectively utilizing unlabeled samples.

•95 volatile compounds are associated with the sweetness of Baijiu.•Sweetness compounds exhibit interactive behavior.•Sweetness mainly attributable to the binding of target molecules to sweet receptors.•The factors affecting the production of sweetness in Baijiu is summarized.•Sweetness impact consumer preferences and related to consumption habits.

A reliable analytical method for the simultaneous determination of famoxadone and oxathiapiprolin dissipation kinetics as well as the metabolites of oxathiapiprolin (IN-E8S72 and IN-WR791) in tomato and soil was developed. We studied the dissipation of famoxadone and oxathiapiprolin in tomatoes grown using different kinetic curves in the area of Beijing in 2015 and 2016. Our results show that the most suitable model for two fungicides in 2015 and 2016 was first-order kinetic and second-order kinetic with the half-lives of 3.4 to 5.2 and 2.4 to 3.0 days, respectively. In addition, we applied the dynamic plant uptake model dynamiCROP and combined it with results from the field experiments to investigate the uptake and translocation of famoxadone and oxathiapiprolin in the soil–tomato environment. Modeled and measured results of two years fitted well with R2values ranging from 0.8072 to 0.9221. The fractions of famoxadone and oxathiapiprolin applied during tomato cultivation that are eventually ingested by humans via residues in crop harvest were finally evaluated and found to be in the range of one part per thousand, that is one gram intake per kilogram applied.

Objective To assess the efficacy of subthreshold micropulse laser photocoagulation (SMLP) therapy versus anti-vascular endothelial growth factor (anti-VEGF) therapy in patients with refractory macular edema (ME) secondary to non-ischemic branch retinal vein occlusion (BRVO).Methods This single-center, prospective, nonrandomized, case-control trial involved patients with refractory ME that responded poorly to three or more initial anti-VEGF injections. The patients were examined and divided into two groups according to their chosen treatment: the intravitreal ranibizumab (IVR) group and the SMLP group. Both groups were followed up monthly for 12 months. Therapeutic efficacy and safety were assessed throughout the follow-up period.Results The IVR group comprised 49 eyes, and the SMLP group comprised 45 eyes. The improvements in the optical coherence tomography findings and visual acuity were comparable between the two groups at the final follow-up. The total number of injections was significantly lower in the SMLP than IVR group. No serious adverse events occurred during the study period.Conclusions SMLP therapy is better for patients with central macular thickness (CMT) of ≤400 μm. For patients with CMT of >400 μm, we advise continuation of anti-VEGF agents to reduce ME followed by application of SMLP therapy when CMT has decreased to ≤400 μm.

The absorption, transport, and subcellular distribution of strobilurin fungicides (azoxystrobin, pyraclostrobin, and trifloxystrobin) have been studied in cucumbers. Under hydroponic laboratory conditions, pyraclostrobin and trifloxystrobin mainly accumulated in cucumber roots whereas azoxystrobin accumulated in cucumber leaves. In the subcellular distribution experiment, azoxystrobin mainly accumulated as a soluble component. Pyraclostrobin and trifloxystrobin accumulated more in the organelles and cell walls. Azoxystrobin and pyraclostrobin enter the root primarily through the apoplast pathway, whereas trifloxystrobin enters the root through the symplastic pathway. Azoxystrobin can be transported in cucumber through anion and cation channels, whereas pyraclostrobin and trifloxystrobin can be transported only through anion channels. This study has great significance in evaluating environmental risks and food safety.

Here, we investigated the uptake, transport, and subcellular distribution of the pesticides pymetrozine and spirotetramat, and spirotetramat metabolites B-enol, B-glu, B-mono, and B-keto, under hydroponic conditions. Spirotetramat and pymetrozine exhibited high bioconcentrations in lettuce roots, with both having root concentration factor (RCF) values >1 after exposure for 24 h. The translocation of pymetrozine from roots to shoots was higher than that of spirotetramat. Pymetrozine is absorbed in roots mainly via the symplastic pathway and is primarily stored in the soluble fraction of lettuce root and shoot cells. The cell wall and soluble fractions were the major enrichment sites of spirotetramat and its metabolites in root cells. Spirotetramat and B-enol were mainly enriched in the soluble fractions of lettuce shoot cells, whereas B-keto and B-glu accumulated in cell walls and organelles, respectively. Both symplastic and apoplastic pathways were involved in spirotetramat absorption. Pymetrozine and spirotetramat uptake by lettuce roots was passive, with no aquaporin-mediated dissimilation or diffusion. The findings of this study enhance our understanding of the transfer of pymetrozine, spirotetramat, and spirotetramat metabolites from the environment to lettuce, and their subsequent bioaccumulation. This study describes a novel approach for the efficient management of lettuce pest control using spirotetramat and pymetrozine. At the same time, it is of great significance to evaluate the food safety and environmental risks of spirotetramat and its metabolites.

Residual fluazinam in the environment may cause dermatitis and occupational asthma. Therefore, it is important to determine the dissipation behavior of fluazinam in edible raw food and in the environment. The aim of this study was to monitor a fungicide fluazinam on potato. A method for the analysis of fluazinam residue and its dissipation in potato plants and soil under field conditions was studied. Fluazinam residues were analyzed using a modified Quick, Easy, Cheap,Effective, Rugged, and Safe (QuEChERS) method and gas chromatography coupled with electron capture detector (GC-ECD). Mean recoveries and relative standard deviations (RSD) in potato plants, potatoes, and soil at three spiking levels were 85.1–99.5 and 0.7–2.8 %, respectively. The limits of quantification (LOQ) were 0.01 mg kg−1for all three matrices. The dissipation dynamics of fluazinam were investigated in field trials in Hebei and Anhui provinces. In potato plants, fluazinam had a half-life of 2.5 days in Hebei and 3.6 days in Anhui. The half-life of fluazinam in soil was 4.7 days in Hebei and 13 days in Anhui. Terminal residues in soil samples ranged from 0.0925 to 0.949 mg · kg−1and fluazinam was not detected in potato at pre-harvest intervals of five, seven, and 10 days. It was safe for fluazinam application on potato according to the recommended dosage and times. Fluazinam residual en el ambiente pudiera causar dermatitis y asma ocupacional. De aquíque es importante determinar el comportamiento de disipación de fluazinam en alimentoscrudos y en el ambiente. El propósito de este estudio fue monitorear un fungicida fluazinamen papa. Se estudió un método para el análisis de residuos de fluazinam y su disipación enplantas de papa y en suelo bajo condiciones de campo. Se analizaron los residuos defluazinam utilizando un método modificado rápido, fácil, barato, efectivo, robusto y seguro(QuEChERS) y cromatografía de gas acoplada con un detector de captura de electrones(GCECD). Las medias de las recuperaciones y las desviaciones estándar relativas (RSD) enplantas de papa, tubérculos y suelo a tres niveles de detección fueron de 85.1--99.5 y 0.7--2.8 % respectivamente. Los límites de cuantificación (LOQ) fueron 0.01 mg kg−1para lastres matrices. Se investigaron las dinámicas de disipación de fluazinam en ensayos decampo en las provincias de Hebei y Anhui. En las plantas de papa fluazinam tuvo una vidamedia de 2.5 días en Hebei y de 3.6 días en Anhui. En el suelo la vida media del productofue de 4.7 días en Hebei y de 13 días en Anhui. Los residuos terminales en las muestras delsuelo fluctuaron de 0.0925 a 0.949 mg kg−1y no se detectó fualzinam en tubérculos aintervalos de pre-cosecha de cinco, siete y diez días. Fue segura la aplicación de fluazinamen papa de acuerdo a la dosis y tiempos recomendados.

ABSTRACTWe developed hemagglutinin- and neuraminidase-specific one-step reverse transcription–loop-mediated isothermal amplification assays for detecting the H10N8 virus. The detection limit of the assays was 10 copies of H10N8 virus, and the assays did not amplify nonspecific RNA. The assays can detect H10N8 virus from chicken samples with high sensitivity and specificity, and they can serve as an effective tool for detecting and monitoring H10N8 virus in live poultry markets.

The Small and Medium sized Enterprises (SMEs) are significantly important part in the national economy. But the employees and decision makers in SMEs are generally lacking of knowledge and skills, this means that the small enterprises have missed out many business opportunities, this is even more evident while implementing and utilising Internet for marketing. So it is necessary to provide the SMEs with knowledge training and decision-making support they needed in Internet marketing. This paper proposed a network based intelligent training system which aims to help SMEs to master knowledge and decision-making skills in using the Internet for marketing. This system contains four main functional modules: Self Evaluation Test (SET), Learning Module (LM), Case Retrieval System (CRS) and Feedback Module (FM). In view of the different knowledge and skill levels of Internet marketing in SMEs, this system provides two training models: theory training and case studies.

The uptake, translocation, and subcellular distribution of oxathiapiprolin and famoxadone in tomato plants were investigated using hydroponic experiments. Oxathiapiprolin and famoxadone mainly accumulated in the tomato roots with limited translocation capacity from the roots to the upper part. The root absorption and inhibitor results noted the dominance of the apoplastic and symplastic pathways in the oxathiapiprolin and famoxadone uptake by the tomato roots, respectively. Furthermore, the uptake process for the two fungicides followed passive and aquaporin-dependent transport. Insoluble cell components (cell organelles and walls) were the dominant storage compartments for oxathiapiprolin and famoxadone. In the protoplast, oxathiapiprolin in the soluble fraction had a higher proportion than that of famoxadone. Finally, the uptake and distribution of the two fungicides by the tomato plants was accurately predicted using a partition-limited model. Thus, this study provides an in-depth understanding of the transfer of oxathiapiprolin and famoxadone from the environment to tomato plants.

Construction of three-dimensional (3D) Cu current collectors is a promising approach to improving the stability of Li metal batteries. Although many efforts have yielded fruitful results, most of them are complex, subtle, and involve high energy consumption. We present a facile chemical fabrication of a 3D current collector which offers the advantages of rapid and low cost assembly by Cu fibers. A skeleton host with a high surface activity can significantly suppress Li dendrite growth and improve the electrochemical deposition behavior of Li. Up to 7 mAh cm?2of Li can be plated in the 3D Cu current collector without dendrite generation. Cells based on 3D Cu collectors achieved a high average Coulombic efficiency of 97% over 160 cycles (50 cycles for planar Cu) and a long cycle life of more than 900 h at 1 mA cm?2. Moreover, full cells assembled with Li-plated 3D Cu anodes and LiFePO4cathodes displayed impressive cycling stability and rate capability at a high rate of 4C.

Sialic acids decorate the surface of glycoproteins and play important roles in a variety of pathological processes. Although the mass spectrometry (MS) based linkage-specific analysis of sialylated N-glycopeptide is developing rapidly, quantitative analysis of these isomers still remains a challenge. Herein, we reported a novel quantitative strategy that can unambiguously identify and relatively quantify linkage-specific N-glycopeptides using ion mobility mass spectrometry (IM-MS). Without the assistance of derivatization, this method can relatively quantify sialic acid isomers of intact glycopeptides by using their characteristic fragment ions in IM-MS. Moreover, good linearity (R2> 0.99) of relative quantification within a dynamic range of 2 orders of magnitude and high reproducibility (coefficient of variation (CV) < 10%, n= 3) were demonstrated. Finally, our results illustrated the aberrant sialylation of haptoglobin (Hp) in hepatocellular carcinoma (HCC), where the ratios of α2,3 to α2,6 sialylation of seven N-glycopeptides were found to be significantly altered (p< 0.01) in HCC individuals (n= 27) compared with healthy controls (n= 27).

Sialylated N-glycan isomers with α-2,3 and α-2,6 linkages play crucial and distinctive roles in diverse physiological and pathological processes. Changes of α-2,3-linked sialic acids in sialylated N-glycans are especially important in monitoring the initiation and progression of diseases. However, the specific analysis of α-2,3-sialylated N-glycan linkage isomers remains challenging due to their extremely low abundance and technical limitations in separation and detection. Herein, we designed an integrated strategy that combines linkage-specific derivatization and a charge-sensitive separation method based on microfluidic chip capillary electrophoresis–mass spectrometry (microchip CE–MS) for specific analysis of α-2,3-sialylated N-glycan linkage isomers for the first time. The α-2,6- and α-2,3-sialic acids were selectively labeled with methylamine (MA) and N,N-dimethylethylenediamine (DMEN), respectively, which selectively makes α-2,3-sialylated N-glycans positively charged and realizes online purification, concentration, and discrimination of α-2,3-sialylated N-glycans from other N-glycans in microchip CE–MS. This new approach was demonstrated with standard multisialylated N-glycans, and it was found that only the α-2,3-sialylated N-glycans migrated and were detected in order according to the number of α-2,3-sialic acids. Finally, this strategy was successfully applied in highly sensitive profiling and reproducible quantitation of the serum α-2,3-sialylated N-glycome from ovarian cancer (OC) patients, where 7 of 33 detected α-2,3-sialylated N-glycans significantly changed in the OC group compared with healthy controls.

The second and third Stokes simultaneous dual-wavelength laser operation at 1320 and 1500 nm based on ?-BaTeMo2O9(?-BTM) crystal was demonstrated. Using an external resonator with a plane-plane configuration, the laser possessed a threshold of 40 MW/cm2at 1064 nm. Maximum output energies of 10.86 mJ (second Stokes) and 9.06 mJ (third Stokes) were obtained at a pump energy of 60 mJ using an output mirror with a transmittance of 44% at 1320 nm. The optical conversion efficiencies from the pump laser to the second and third Stokes lasers were about 18.1 and 15.1%, respectively.

The second and third Stokes simultaneous dual-wavelength laser operation at 1320 and 1500 nm based on b-BaTeMo2O9 (b-BTM) crystal was demonstrated. Using an external resonator with a plane-plane configuration, the laser possessed a threshold of 40 MW/cm2 at 1064 nm. Maximum output energies of 10.86 mJ (second Stokes) and 9.06 mJ (third Stokes) were obtained at a pump energy of 60 mJ using an output mirror with a transmittance of 44% at 1320 nm. The optical conversion efficiencies from the pump laser to the second and third Stokes lasers were about 18.1 and 15.1%, respectively.