Search

The article offers information on the transition from blanket treatment of cows with antibiotic dry cow therapy (DCT) to selective treatment (ST) using internal teat sealant (ITS) or antibiotics based on individual somatic cell count (ISCC) and mastitis history. The study examines the implementation of ST dry off in a dairy partnership and analyzes the data to assess the impact on bulk tank somatic cell count (BTSCC) and mastitis control.

Two iboga-vobasine bisindoles, 16′-decarbomethoxyvoacamine (1) and its 19,20-dihydro derivative, 16′-decarbomethoxydihydrovoacamine (2) from Tabernaemontana corymbosa exhibited potent cytotoxicity against the human colorectal adenocarcinoma HT-29 cells in our previous studies. Bisindoles 1 and 2 selectively inhibited the growth of HT-29 cells without significant cytotoxicity to normal human colon fibroblasts CCD-18Co. Treatment with bisindoles 1 and 2 suppressed the formation of HT-29 colonies via G0/G1 cell cycle arrest and induction of mitochondrial apoptosis. Owing to its higher antiproliferative activity, bisindole 2 was chosen for the subsequent studies. Bisindole 2 inhibited the formation of HT-29 spheroids (tumor-like cell aggregates) in 3D experiments in a dose-dependent manner, while an in vitro tubulin polymerization assay and molecular docking analysis showed that bisindole 2 is a microtubule-stabilizing agent which is predicted to bind at the β -tubulin subunit at the taxol-binding site. The binding resulted in the generation of ROS, which consequently activated the oxidative stress-related cell cycle arrest and apoptotic pathways, viz., JNK/p38, p21Cip1 /Chk1, and p21Cip1 /Rb/E2F, as shown by microarray profiling. [ABSTRACT FROM AUTHOR]

The aim of the present study was to investigate the cytotoxic effects and underlying molecular mechanisms of nitidine chloride (NC) in hepatocellular carcinoma cells via quantitative proteomics. MTT assays were used to detect the inhibitory effects of NC in Bel-7402 liver cancer cells, and the number of apoptotic cells was measured by flow cytometry. Quantitative proteomics technology based on iTRAQ was used to discover differential expressed proteins after NC treatment, and bioinformatic techniques were further used to screen potential targets of NC. Molecular docking was applied to evaluate the docking activity of NC with possible upstream proteins, and their expression was detected at the mRNA and protein levels by quantitative reverse transcription PCR and western blotting. NC inhibited the proliferation of Bel-7402 cells after 24 h of treatment and stimulated apoptosis in vitro. The proteomics experiment showed that NC triggers mitochondrial damage in HCC cells and transcription factor AP-1 (c-Jun) may be a potential target of NC (fold change = 4.36 ± 0.23). Molecular docking results revealed the highest docking score of NC with c-Jun N-terminal kinase (JNK), one of the upstream proteins of c-Jun. Moreover, the mRNA and protein expression of c-Jun and JNK were significantly increased after NC treatment (p < 0.05). These findings indicate that NC significantly induced mitochondrial damage in HCC cells, and induced apoptosis by activating JNK/c-Jun signaling. [ABSTRACT FROM AUTHOR]

The liverwort Radula perrottetii contains various bibenzyl derivatives which are known to possess various biological activities, such as anti-inflammatory effects. Mast cells (MC) play crucial roles in allergic and inflammatory diseases; thus, inhibition of MC activation is pivotal for the treatment of allergic and inflammatory disorders. We investigated the effects of perrottetin D (perD), isolated from Radula perrottetii , and perD diacetate (Ac-perD) on antigen-induced activation of MCs. Bone marrow–derived MCs (BMMCs) were generated from C57BL/6 mice. The degranulation ratio, histamine release, and the interleukin (IL)-4 and leukotriene B4 productions on antigen-triggered BMMC were investigated. Additionally, the effects of the bibenzyls on binding of IgE to Fc ε RI were observed by flow cytometry, and signal transduction proteins was examined by Western blot. Furthermore, binding of the bibenzyls to the Fyn kinase domain was calculated. At 10 µM, perD decreased the degranulation ratio (p < 0.01), whereas 10 µM Ac-perD down-regulated IL-4 production (p < 0.05) in addition to decreasing the degranulation ratio (p < 0.01). Both compounds tended to decrease histamine release at a concentration of 10 µM. Although 10 µM perD reduced only Syk phosphorylation, 10 µM Ac-perD diminished phosphorylation of Syk, Gab2, PLC- γ , and p38. PerD appeared to selectively bind Fyn, whereas Ac-perD appeared to act as a weak but broad-spectrum inhibitor of kinases, including Fyn. In conclusion, perD and Ac-perD suppressed the phosphorylation of signal transduction molecules downstream of the Fc ε RI and consequently inhibited degranulation, and/or IL-4 production. These may be beneficial potential lead compounds for the development of novel anti-allergic and anti-inflammatory drugs. [ABSTRACT FROM AUTHOR]

A recent study aimed to develop a method for detecting ASC aggregates originating from NLRP3 inflammasomes, which are involved in inflammatory diseases. The researchers used flow cytometry to validate the detection of ASC/NLRP3-positive events in human monocytes, macrophages, and THP-1 ASC reporter cells. They confirmed the presence of ASC/NLRP3 specks in cell supernatants and found elevated levels of these specks in patient sera from inflammatory conditions compared to healthy controls. The study suggests that flow cytometry can be a reliable method for detecting ASC/NLRP3 specks in human sera, potentially aiding in the diagnosis and monitoring of certain systemic autoinflammatory diseases. [Extracted from the article]

A recent study conducted by the Center for Cooperative Research in Biosciences has found that there are increased levels of circulating cell-free double-stranded nucleic acids in the plasma of glioblastoma patients. This discovery is significant because circulating cell-free nucleic acids are considered a promising source of biomarkers for diseases and cancer. The researchers developed a method using conventional flow cytometry to analyze these nucleic acids in plasma and extracellular vesicles (EVs), which could be easily translated into clinical practice. The study also revealed differences in nucleic acid profiles between patients and healthy individuals, suggesting that this methodology has potential as a tool for plasma nucleic acid analysis. [Extracted from the article]

The article focuses on a study conducted at Peking University in China, developing a nanoparticle-based tumor vaccine, OVA@HA-PEI, to enhance immune response against tumors. The study found that OVA@HA-PEI, combined with an anti-programmed cell death protein 1 (PD-1) antibody, significantly improved survival rates, reduced circulating tumor cells, and slowed tumor progression in mice.

According to a preprint abstract from biorxiv.org, spectral flow cytometry is a widely used tool for cell analysis. However, when using spectral cytometry on cell sorters, it is important to consider the mismatch of buffers used for sample suspension and sort collection. This mismatch can affect the accuracy of purity check results, potentially leading to erroneous conclusions. The research has not yet undergone peer review. For more information, refer to the original article on biorxiv.org. [Extracted from the article]

A recent study has developed a new method called ImmunoCellCycle-ID for identifying the different stages of the cell cycle with high precision. The traditional method of flow cytometry requires a large number of cells, whereas this new method provides single-cell resolution using immunofluorescence and fluorescence microscopy. The method can be used to study the detailed sub-stages of the cell cycle in various cell lines and may serve as an alternative or complement to flow cytometry. However, it is important to note that this study has not yet undergone peer review. [Extracted from the article]

This article discusses the development of a toolbox of biosensors for the second messenger c-di-GMP, which plays a role in coordinating various cellular functions in bacteria. The researchers constructed a library of biosensors and characterized them using flow cytometry. They then combined this toolbox with a transposon library and cell sorting to create a new application called FRET-To-Sort, which allows for the systematic characterization of gene networks regulating c-di-GMP levels. The application was applied to E. coli cells and identified both known and novel regulatory modules. This research has not yet undergone peer review. [Extracted from the article]

A preprint abstract from biorxiv.org discusses the challenges of fixation and permeabilization in flow cytometry, which can alter fragile targets and compromise data quality. The authors propose a multi-pass flow cytometry approach that uses individual cell barcoding with laser particles to sequentially analyze the same cells while maintaining single-cell resolution. This technique allows for accurate measurement of intracellular biomarkers and chemically-sensitive fluorescent labels, enhancing assay flexibility and opening up new possibilities in immuno-oncology, stem cell research, and cell biology. The preprint has not yet undergone peer review. [Extracted from the article]

A recent study conducted at the University of Shanghai for Science and Technology in China has developed a modular fluorescence flow cytometry (M-FCM) system that allows for flexible arrangement of fluorescence channels. This system eliminates crosstalk among the channels and provides a multifunctional platform for various biophysical measurements. The M-FCM system was successfully used to evaluate cell viability in drug screening and showed promising results. This research opens up new possibilities for the multivariate analysis of cellular characteristics and has the potential for future upgrades and applications in different fields. [Extracted from the article]

A report from the Universidad Carlos III de Madrid discusses the challenges of using flow cytometry in mice for immunology studies due to limited availability of mouse-specific antibodies and the need to work with small volumes of peripheral blood. To address these challenges, the researchers developed two novel flow cytometry panels that allow for extensive analysis of immune cell populations in mice using only 50 L of peripheral blood per panel. These panels were validated on a lung inflammation model and were found to be sensitive enough to detect changes in peripheral blood. The researchers concluded that these panels provide a robust platform for longitudinal studies in mice and can uncover significant insights into immune responses. [Extracted from the article]

ImpriMed, a precision medicine startup, has published groundbreaking research on a new cell-sizing method for feline lymphomas in Veterinary Sciences. This method improves the precision of cell size assessment, potentially transforming veterinary oncology by providing more reliable prognostic insights and enhancing treatment planning and patient outcomes. The study highlights the effectiveness of ImpriMed's flow cytometry-based approach in distinguishing between small, medium, and large cell sizes, which correlates with patient survival rates. This research addresses the unmet needs of feline cancer patients and lays the groundwork for future studies on AI-driven prognostication for feline lymphoma. [Extracted from the article]

A recent study explored the potential of urinary extracellular vesicles (uEVs) as non-invasive biomarkers for assessing kidney health. The study focused on kidney-derived uEVs (kd-uEVs) and their correlation with kidney function. Using imaging flow cytometry, researchers identified single kd-uEVs labeled with CD63, a uEV marker, in combination with either PODXL, a podocyte marker, or AQP2, a tubular marker. While no significant association was found between the concentration of CD63+ AQP2+ or PODXL+ uEVs and kidney function, a significant correlation was observed between AQP2+ and PODXL+ uEV concentrations. This correlation may be explained by the colocalization of AQP2 and PODXL on CD63+ uEVs. This study is the first to demonstrate the colocalization of podocyte and tubular proteins on uEVs. [Extracted from the article]

A researcher from Korea University has conducted a study on the effects of fine dust and the potential benefits of erucic acid-induced exosomes (EIEs) in preventing environment-borne diseases. Fine dust has been linked to various disorders, including cardiovascular, neurological, renal, reproductive, motor, systemic, respiratory, and cancerous diseases. The study found that erucic acid effectively suppresses inflammation and promotes osteogenic activators in fibroblasts exposed to fine dust. EIEs were also found to enhance osteocytic differentiation and phagocytosis in macrophages, indicating their potential as biomaterials for preventing environment-borne diseases. [Extracted from the article]

A recent study conducted by researchers at Western Michigan University explores the use of flow cytometry to analyze retinal cells in mice. The study focuses on the potential of a selective alpha7 nicotinic acetylcholine receptor agonist, PNU-282987, to increase the number of retinal neurons in adult mice. The researchers developed a flow cytometry method that allows for the labeling and analysis of multiple retinal cell types. This study provides a step-by-step protocol for the labeling of retinal ganglion cells, rod photoreceptors, Muller glia, and Muller glia-derived progenitor cells. [Extracted from the article]

A preprint abstract from biorxiv.org discusses the immune regulatory protein VISTA and its interaction with its ligands. The study challenges the prevailing belief that VISTA interacts with its ligands between cells (in trans), instead finding that it interacts with its ligands within the same cell (in cis). The researchers propose that a disulfide bond causes VISTA's Ig domain to bend towards the cell membrane, preventing trans interactions while enabling cis interactions. The study suggests that this new understanding of VISTA's binding mechanism could lead to the development of novel VISTA-targeted therapeutics. [Extracted from the article]

A recent report published by the Department of Medicine discusses a case of T-cell prolymphocytic leukemia (T-PLL), a rare and aggressive form of leukemia. However, in this particular case, the patient presented with an indolent course, meaning the disease progressed slowly and the patient remained asymptomatic. The diagnosis was made based on clinical features, lymphocyte morphology, and flow cytometry. The patient was closely monitored for at least one year due to the asymptomatic nature of the disease. This research provides valuable insights into the understanding and management of T-PLL. [Extracted from the article]

The article offers information on a study investigating cross-reactive anti-Soluble-Liver-Antigen (SLA) antibodies in the context of allosensitisation using non-human primates. Topics discussed include the limitations of lymphocyte-based flow cytometry crossmatches in detecting these antibodies; the development of a major histocompatibility complex (MHC) Matchmaker algorithm to predict cross-reactivity; and the use of serum samples from sensitised non-human primates to assess antibody binding.

A recent study conducted by ImpriMed Inc. in Mountain View, California, has investigated the use of flow cytometry and clonality analysis for predicting prognosis and characterizing feline lymphoma. The study analyzed fine needle aspirates and blood samples from 438 feline patients and found that the proposed methodology achieved high concordance with cytological evaluations. Additionally, the study demonstrated significant survival differences among lymphoma subtypes defined by flow cytometry and clonality analysis, highlighting the prognostic utility of these methods. This research provides valuable insights for the characterization and management of feline lymphoproliferative diseases. [Extracted from the article]

A preprint abstract from biorxiv.org discusses the use of flow cytometry as a potential alternative to traditional methods for determining bacterial community composition in synthetic communities. The study compared flow cytometry to strain specific qPCR and 16S rRNA gene amplicon sequencing in axenic cultures, mock communities, and co-cultures of oral bacteria. Flow cytometry showed better performance than the PCR-based methods in mock communities, but its accuracy was reduced in co-cultures due to changing phenotypes. The study highlights the challenges of using flow cytometry to quantify community composition in co-cultures. This preprint has not yet undergone peer review. [Extracted from the article]

Dotmatics, a leader in R&D scientific software, has acquired EasyPanel, a company specializing in flow cytometry panel design software. This acquisition expands Dotmatics' flow cytometry solutions and adds panel design capabilities to its existing offerings. EasyPanel's intelligent and automated flow cytometry panel designer suggests optimized panels based on a customer's test environment, allowing for improved experimental impact. The acquisition supports Dotmatics' commitment to delivering reliable machine learning and AI tools for advanced automation of flow cytometry analysis. [Extracted from the article]

Researchers at Hospital Universitario Ramon y Cajal in Madrid, Spain have evaluated the performance of a flow cytometry-based rapid assay for the detection of carbapenemase-producing Enterobacterales, which are increasingly recognized in nosocomial infections. The study compared the results of the assay with molecular and phenotypic methods and found that the flow cytometry assay showed high agreement with the reference method for carbapenemase detection. The researchers concluded that this new technology has high accuracy for carbapenemase detection and differentiation, which could have a significant impact on clinical microbiology laboratories and patient management. [Extracted from the article]

A recent study from McMaster University in Canada explores advancements in microflow cytometry technology for diagnosing myelodysplastic syndromes (MDS), a rare form of early-stage blood cancer. The study highlights the limitations of current diagnostic methods, such as blood tests, bone marrow biopsies, and genetic analysis, and suggests that microflow cytometry integrated with microfluidics could address these shortcomings. The benefits of this technology include portability, processing speed, and lower operating costs, making it a promising point-of-care diagnostic device for MDS and other blood cancers. Further information on this research can be found in the journal article linked in the source. [Extracted from the article]

MuPET-Flow is a new tool developed to automate the estimation of ploidy, which represents the number of homologous chromosome sets, from flow cytometry data. This tool allows users to visualize cell fluorescence histograms, detect different cell cycle phases, make ploidy predictions, and export results. It has been benchmarked with known ploidy datasets and produces consistent results comparable to other available tools. MuPET-Flow is particularly useful for projects with large numbers of samples due to its user-friendly interface, which speeds up the analysis process. However, it is important to note that this preprint has not yet been peer-reviewed. [Extracted from the article]

A recent study conducted by researchers at the University of Bucharest in Romania has developed a label-free flow cytometry (FCM) method for rapidly evaluating the bactericidal activity of disinfectants. The study compared the efficacy of this method with standard qualitative/quantitative suspension tests and found that the FCM measurements showed excellent correlation with the standard assays. This label-free FCM protocol offers a novel and rapid tool for environmental health experts to optimize disinfectant selection for the prevention and control of healthcare-associated infections (HAIs). The research provides valuable insights for infection prevention and control procedures in healthcare environments. [Extracted from the article]

Researchers at Tallinn University of Technology in Estonia have developed a lightweight imaging pipeline for acquiring blur-free single droplet images in imaging flow cytometry (IFC). The pipeline utilizes minimum hardware and a lightweight algorithm to detect, count, and acquire single object images from a video stream. The proposed pipeline achieved 100% counting accuracy and processing times of 2 ms, 25 ms, and 10 ms for each droplet on a desktop PC, Raspberry Pi-4, and Nvidia Jetson Nano, respectively. The researchers concluded that the pipeline is suitable for efficient single object image acquisition in IFC on an embedded portable platform. [Extracted from the article]

A researcher from Zhejiang University has published a report on the release characteristics of small-sized microplastics (MPs) in bottled drinks using flow cytometry sorting and Nile Red staining. The study found that temperatures below 80 °C had little effect on the release of MPs from PET bottles, while sunlight irradiation and alkalinity promoted the generation of MPs, mostly 1-5 mm in size. The combined impact of acidity with temperature and alkalinity with sunlight positively affected MP release. The results of this study can assist researchers in reducing MP release and evaluating MPs' contamination of aquatic environments. [Extracted from the article]

A recent study conducted by researchers at Beckman Coulter Life Sciences explores the immune system and its role in protecting the body against pathogens and abnormalities like cancer cells. The study focuses on how the immune system responds to initial encounters with antigens and how it ages over time, a process known as immunosenescence. The researchers highlight the use of advanced technologies like flow cytometry in enhancing our understanding of the immune system's behavior and discuss the assessment of extracellular vesicles and their implications for immune system functioning. The study provides valuable insights into the immune system's development and functioning throughout a person's lifetime. [Extracted from the article]

A recent study conducted in Szombathely, Hungary, examined the use of flow cytometry (FCM) in the diagnosis of myelodysplastic neoplasms (MDS) with low blasts and cytopenia. The researchers analyzed bone marrow samples from 179 patients with cytopenia and found that the combination of the Ogata score and the "3 aberrations in two cell compartments method" had the highest diagnostic accuracy (78%). The study concluded that FCM is an important but supplementary tool in the diagnosis of MDS with low blasts, and the combination of these two methods slightly improves accuracy. [Extracted from the article]

The University of Southampton has been issued a patent for an impedance flow cytometry apparatus. This invention aims to address the issue of antimicrobial resistance by providing a faster and more cost-effective method for analyzing antimicrobial testing. The apparatus uses electrodes and current paths to detect current flow in a fluid containing particles suspended in an electrolyte, allowing for the determination of impedance properties and the identification of particles. This technology has the potential to guide appropriate antimicrobial prescription and improve patient treatment outcomes. [Extracted from the article]

A recent report from Weill Cornell Medical College discusses the role of flow cytometry in diagnosing and making clinical decisions about germline hematologic neoplasms, a distinct category of tumors. The study highlights the need for molecular testing and a high index of suspicion when dealing with these tumors. The researchers review the correlation between flow cytometric immunophenotype and germline hematologic neoplasms, focusing on commonly mutated genes and the potential utility of flow cytometry in diagnosis and screening. However, further studies are needed to better understand the flow cytometric patterns of these neoplasms. [Extracted from the article]

This article discusses the need for simultaneous quantification of yeast and bacterial cells in industrial bioprocesses, such as the food and bioethanol industries. Traditional methods for determining cell concentrations individually are time-consuming and resource-intensive. The study compared different methods for quantifying yeast and bacterial cells in microbial suspensions and found that each method has its limitations, advantages, and disadvantages. The best option for quantification will depend on the specific application. The article provides a comparison of the techniques in terms of time-to-results, cost of analysis and equipment, range of detectable cell/particle diameters, adequacy for simultaneous enumeration, and general pros and cons. [Extracted from the article]

This article discusses a patent application for a flow cytometry system and methods. Flow cytometry is a technology used for cell counting, cell sorting, biomarker detection, and protein engineering. The patent application describes improvements to high-throughput flow cytometry systems, including the use of lasers, side scatter detection modules, and a fluidic pathway. The system is designed to detect and analyze multiple samples simultaneously, allowing for faster and more efficient analysis. The patent application also includes a flow cell module and a system that combines the flow cytometry system with the flow cell module. [Extracted from the article]

A recent study conducted at the Medical University of Warsaw in Poland compared the results of lymphocyte immunophenotyping between two different flow cytometers. The study found that the results from both cytometers were considered equivalent, although there were some deviations due to different gating techniques used by the evaluators. The researchers concluded that more attention should be given to the training of specialists performing these tests. This study provides valuable information for laboratories using flow cytometry immunophenotyping as a diagnostic tool in various medical fields. [Extracted from the article]

The "Organoids, Spheroids & Organs-on-Chips 2024" conference, hosted by ResearchAndMarkets.com, focuses on the field of assembling cells using Microfluidics and Lab-on-a-Chip technologies. These assembled cells can mimic the structure of organs and have functional significance. The conference will explore various chips, such as Lung-on-a-Chip, Brain-on-a-Chip, and Gut-on-a-Chip, as well as disease-on-a-chip and cancer-on-a-chip models. The conference offers networking and business development opportunities. Speakers include experts from prestigious institutions such as the University of California and Massachusetts Institute of Technology. For more information, visit the ResearchAndMarkets.com website. [Extracted from the article]

This article discusses the development of a spectral flow cytometry analysis pipeline for high-dimensional immune cell characterization. The authors explain that flow cytometry is a commonly used technique for studying immune cells, but analyzing data from large antibody panels can be challenging. They present a novel analysis pipeline that improves the analysis of spectral flow cytometry data and use it to identify rare T cell populations in aging. The study reveals significant differences between young and aged mice in terms of T cell subsets, providing insights into the immune aging process. The authors suggest that this approach could facilitate the discovery of novel therapeutic targets for age-related immune dysfunction. [Extracted from the article]

A new study published in Veterinary Week discusses the adaptation of SMART tube technology for flow cytometry in feline full blood samples. Flow cytometry is a valuable tool for monitoring immune response in human patients and has been successfully applied in cats. The SMART Tube system allows for the fixation and long-term preservation of whole blood samples, and this study successfully established a protocol for using this system with feline samples. The results showed that the adapted protocol is suitable for performing flow cytometry analysis on stored feline whole blood samples, opening up new avenues for veterinary research. [Extracted from the article]

A recent study conducted at the University of Montreal has reported new findings on cytokines. The study focused on the detection of a specific protein called Cardiotrophin-like cytokine factor 1 (CLCF1) using a technique called flow cytometry. The researchers successfully detected CLCF1 protein expression in various immune cells, including pro-B cells and T cells. This discovery provides a valuable tool for studying CLCF1 expression in normal and pathological immune responses. The study was funded by the Canadian Institutes of Health Research. [Extracted from the article]

Telomeres are repetitive DNA sequences at the end of chromosomes that protect them from damage. Maintaining the length of telomeres is important for preventing age-related degenerative diseases and cancer. Telomere profiling is a new method that accurately measures telomere length using sequencing technology. This method has important implications for understanding human disease and can be used in research and clinical labs at manageable costs. Another study focused on the increased risk of leukemia in Hispanic and Latino children and identified a variant in the IKZF1 gene that may contribute to this risk. Further research is needed to understand the biological impact of this variant. Anil Parwani, MD, PhD, MBA, has been appointed as the Donald A. Senhauser, MD, Chair in Pathology at Ohio State University College of Medicine. [Extracted from the article]

A recent report from Aarhus University Hospital in Denmark discusses research on acute myeloid leukemia (AML), a type of blood cancer with a poor prognosis. The researchers used imaging flow cytometry and convolutional neural network-based classification to distinguish between hematopoietic stem cells (HSCs) and leukemic stem cells (LSCs) based on their morphology. The study found that combining brightfield and DNA images achieved 93.42% accuracy in differentiating HSCs from LSCs. These findings demonstrate the potential for a novel technique in monitoring AML. [Extracted from the article]

IDEX Health & Science has launched the Semrock line of Nanopede Optical Filters for flow cytometry applications. These filters cover the UV, visible, and NIR spectrum in discrete steps, allowing for spectral flow cytometry. The company also released the Melles Griot XPLAN CCG Lens Series, which provides custom imaging performance with shorter lead times. Additionally, Automation Technology GmbH has introduced the ECS series of 3D sensors for the food and logistics industries, and Mitsui Chemicals offers the Diffrar series of polymer wafers for waveguides used in AR glasses. OFS has debuted the OM4+ fiber for multimode, bi-directional network applications. Finally, ICFO Director Lluis Torner has been awarded Barcelona's Gold Medal for Scientific Merit. [Extracted from the article]

Cancer is one of the major causes of death worldwide. In addition to standard regimens, tumor suppression ability has been demonstrated in many types of natural products, including Piper nigrum , or black pepper. In previous reports, we demonstrated the antitumor effect of low piperine fractional Piper nigrum extract in vitro and in vivo. However, the effects of low piperine fractional P. nigrum extract in the aspect of antitumor immunity has not yet been investigated. In this study, tumor-bearing rats were fed with 100 mg/kg BW or 200 mg/kg BW of low piperine fractional P. nigrum extract 3 times per week for 4 weeks. Tumor burden and hematological data were then evaluated. Immunological data was investigated using a cytokine array and flow cytometry. The results showed that both doses of low piperine fractional P. nigrum extract significantly suppressed tumor progression in N-nitrosomethylurea-induced mammary tumor rats. There were no significant changes observed in the total white blood cells, red blood cells, and hemoglobin. Low piperine fractional P. nigrum extract suppressed some cytokine and chemokine levels including CXCL7, sICAM-1, and L-selectin 0.2- to 0.6-fold. Interestingly, 200 mg/kg BW of low piperine fractional P. nigrum extract significantly promoted type 1 T helper cell, and suppressed neutrophil, basophil, type 2 T helper cell, and regulatory T cell compared to the control group. In summary, these results indicate that low piperine fractional P. nigrum extract had a high efficacy in supporting antitumor activity at immunological levels via regulating Th1/Th2/Treg cells. [ABSTRACT FROM AUTHOR]

This article discusses the need for quantitative cell analysis in liquid biopsies for clinical decisions, particularly in low-resource settings or for immobile patients where access to centralized laboratories is limited. The article explores the use of magnetic flow cytometers (MFC) as a solution for point-of-care (POC) testing, but notes that sample processing steps like cell lysis or washing can disrupt POC-capable MFC workflows. The researchers investigate conditions for immunomagnetic labeling and direct cell quantification in high hematocrit environments, using magnetic nanoparticles (MNP) characterized by their size and magnetic moment. The article concludes by demonstrating reliable POC flow cytometry in whole blood with biomimetic microbeads and achieving regulatory standards for quantifying CD14+ monocytes. [Extracted from the article]

A clinical trial, NCT06452732, is being conducted to investigate the prediction of relapse in pediatric acute myeloid leukemia. The trial will use multiparametric flow cytometry to detect leukemia stem cells in peripheral blood and bone marrow. The goal is to improve upon the current markers for relapse prediction, which have high rates of false negatives and false positives. The trial will assess various outcomes, including relapse rates, survival rates, and incidences of complications. The researchers conducting the study have discovered that circulating leukemia stem cells play a role in relapse after chemotherapy, and they plan to monitor these cells to predict relapse. The study will involve newly diagnosed patients who are 18 years old or younger and able to provide informed consent. The trial is not yet recruiting participants and is expected to be completed by June 2026. [Extracted from the article]

A patent application titled "Systems And Methods For Panel Design In Flow Cytometry" has been submitted by inventor Michael Kapinsky. The application relates to techniques for selecting antibody-dye conjugate combinations for use in flow cytometry, as well as automated systems and methods for analyzing positivity in multicolor flow cytometry. The invention aims to improve the efficiency and sensitivity of flow cytometry analysis, allowing for the simultaneous detection of multiple fluorescent markers. The patent application provides detailed methods and systems for determining optimized probe panels and assessing positivity in flow cytometry. [Extracted from the article]

A report from the University of Liege in Belgium discusses research on the role of Ly6Chi monocytes in shaping the function of effector CD4+ T cells during a murine gammaherpesvirus infection. The researchers used multiparametric flow cytometry to analyze the functional properties of lung-infiltrating CD4+ T cells after viral infection. The study provides a detailed protocol for mouse lung dissection, preparation of single-cell suspension, and staining for flow cytometry analysis. The research aims to better understand the regulation of CD4+ T cells to prevent virus-induced immunopathologies in the lung. [Extracted from the article]

A recent study conducted at Children's Hospital of Michigan explored the use of flow cytometry as a tool to define peripheral blood leukocyte signatures in acute Epstein-Barr virus (EBV) infection. The researchers observed novel alterations in lymphocyte, neutrophil, and monocyte populations in patients with infectious mononucleosis (IM), a manifestation of primary EBV infection. These findings may contribute to a better understanding of IM immunobiology and could be useful in investigating conditions characterized by deficiencies in controlling EBV infection. The study was supported by the Children's Foundation and was published in the journal Cells. [Extracted from the article]

Researchers from Augusta University have published a new study on biomarkers in the area of myeloid cells in mouse retina research. The study focuses on the challenges of assessing the myeloid cell response after retinal injury in mice, specifically distinguishing between microglia and infiltrating macrophages. The researchers describe a flow cytometry-based protocol that can analyze the myeloid cell response in retinopathy mouse models and can be modified to incorporate other markers of interest. The study provides representative results from three different retinopathy models. [Extracted from the article]