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A recent report from Konkuk University in Seoul, South Korea, presents new data on the biodegradation of azo dye Reactive Black 5 (RB5) by the bacterium Clostridium acetobutylicum. The study found that C. acetobutylicum showed the best decolorization of RB5 among the hydrogen-producing Clostridium species tested. The researchers also discovered that C. acetobutylicum could simultaneously degrade RB5 and produce biohydrogen. The study suggests that C. acetobutylicum has potential applications in wastewater treatment for simultaneous decolorization and biohydrogen production. [Extracted from the article]

Researchers from the Chinese Academy of Sciences have conducted a study on the Gram-Positive Bacteria Clostridium acetobutylicum. The study explores the potential of butanol fermentation from sweet potato waste as a renewable green liquid fuel. The researchers found that simultaneous viscosity reduction and fermentation significantly improved the fermentation performance of Clostridium acetobutylicum, resulting in increased butanol concentration. The study demonstrates the feasibility of utilizing sweet potato waste as a feedstock for butanol fermentation, providing a novel strategy for reducing environmental pollution and recycling waste biomass. [Extracted from the article]

A study conducted by the Council of Scientific and Industrial Research (CSIR) Indian Institute of Chemical Technology in Hyderabad, India, explored the production of biobutanol using Clostridium acetobutylicum ATCC-824. The researchers investigated how changes in the extracellular redox potential affected the production of acetone, butanol, and ethanol during bi-phasic fermentation. The study found that altering the redox potential resulted in increased butanol and acetone production compared to conventional fermentation. Gene expression and network analysis also revealed differences between the two fermentation methods. The research suggests that regulating electron flow can impact the production of specific metabolites in bi-phasic fermentation. [Extracted from the article]

A study conducted by researchers at Dalian Polytechnic University in Liaoning, China, has developed a new method for multiplexed detection of hazardous factors in food safety and public health. The study focuses on the use of a mesophilic Clostridium butyricum Argonaute (CbAgo)-Powered Programmable (APP) platform combined with a magnetic nanoparticle tetrahedral DNA encoding system to detect multiple non-nucleic acid targets at 37°C. The platform showed promising results in detecting mycotoxins in food samples and pathogens in clinical samples, indicating its potential as a multiplexed and ultrasensitive detection tool in various fields. The research has been peer-reviewed and published in the Chemical Engineering Journal. [Extracted from the article]

Researchers from Sakarya University in Turkey have conducted a study on the fermentation of acetone, butanol, and ethanol (ABE) using fresh and rotten potatoes. Three strains of Clostridium bacteria were used in the production experiments. The results showed that rotten potatoes could be a suitable substrate for ABE fermentation, with significant amounts of ABE produced. The study provides valuable insights into the efficient production of ABE from potatoes and highlights the potential of using rotten potatoes as a resource for biofuel production. [Extracted from the article]

A study conducted by researchers at Polytechnic University Torino in Turin, Italy, has investigated the influence of acetate concentration on acetone production by a modified strain of the bacteria Acetobacterium woodii. Acetone is a widely used chemical compound, and the demand for it is increasing globally. The study found that acetone synthesis was stimulated when the concentration of acetate in the medium exceeded a certain threshold. This information is important for the design of fermentation processes, particularly in continuous operative mode. The research was supported by the European Union and has been peer-reviewed. [Extracted from the article]

Researchers from Riga Technical University in Latvia have conducted a study on Clostridium beijerinckii, a type of bacteria known for its ability to produce butanol, a potential alternative to fossil fuels. The study aimed to optimize the process of butanol production by testing the effects of temperature, pH, and agitation on three strains of bacteria. The optimal conditions for C. beijerinckii were found to be a temperature of 32 °C, no agitation for growth, and an agitation speed of 200 rpm for butanol production. The study also provided optimal conditions for two other strains of bacteria. This research contributes to the understanding of optimal growth conditions for butanol production. [Extracted from the article]

A recent study conducted by researchers at Aichi Medical University explored the effects of orally administered Clostridium butyricum on endometritis, a condition that can negatively impact fertility and cause preterm parturition syndrome. The study found that Clostridium butyricum exhibited anti-inflammatory effects on uterine tissues. The researchers also discovered that the administration of Clostridium butyricum altered the uterine microbiome and induced the proliferation of certain bacterial species, which contributed to the anti-inflammatory effects. These findings provide insights into the treatment and prevention of endometritis. [Extracted from the article]

A recent study conducted by researchers at the University of Toulouse in France has made advancements in the field of gene editing. The study focused on improving the genome editing tools used in Clostridium acetobutylicum, a type of bacteria. The researchers developed a method that allows for genome modification without leaving any markers or scars in the bacteria strain. They also addressed the issue of the strain becoming resistant to the selection method used, 5-fluorouracil (5FU), by creating a corrective replicative plasmid. Additionally, the researchers optimized the system by reducing the concentration of 5FU and successfully replaced a gene in the bacteria. This research has implications for biotechnology and genetics. [Extracted from the article]

Researchers from the University of Girona have developed a multiplex PCR method for the simultaneous quantification of three species of Clostridium bacteria in co-cultures. The method targets the 16S rRNA gene and allows for the specific quantification of Clostridium acetobutylicum, Clostridium carboxidivorans, and Clostridium cellulovorans without cross-reactions. The researchers found that combining C. carboxidivorans and C. acetobutylicum resulted in higher alcohol production compared to individual cultures, and further improved in a triplet consortium. This methodology could be applied to fermentation processes targeting butanol production from lignocellulosic feedstocks with higher substrate conversion efficiency. [Extracted from the article]

A report from Nanjing Tech University in China discusses the mining and application of lipase from Clostridium acetobutylicum for the production of butyl butyrate. Butyl butyrate is commonly used in industries such as beverages, fragrances, food, and cosmetics. The researchers successfully displayed the lipase on the surface of Escherichia coli, resulting in a higher catalytic efficiency and specificity for butyl butyrate synthesis compared to commercial enzymes. The study suggests that this cell surface-displayed lipase could eliminate the need for commercial lipase in the biosynthesis of butyl butyrate. [Extracted from the article]

Keywords for this news article include: Hashemite University, Health and Medicine, Gram-Positive Bacteria, Clostridium acetobutylicum, Gram-Positive Endospore-Forming Rods, Gram-Positive Endospore-Forming Bacteria. Clostridium, Clostridium acetobutylicum, Gram-Positive Bacteria, Gram-Positive Endospore-Forming Bacteria, Gram-Positive Endospore-Forming Rods, Health and Medicine Keywords: Clostridium; Clostridium acetobutylicum; Gram-Positive Bacteria; Gram-Positive Endospore-Forming Bacteria; Gram-Positive Endospore-Forming Rods; Health and Medicine EN Clostridium Clostridium acetobutylicum Gram-Positive Bacteria Gram-Positive Endospore-Forming Bacteria Gram-Positive Endospore-Forming Rods Health and Medicine 2530 2530 1 10/30/23 20231103 NES 231103 2023 NOV 3 (NewsRx) -- By a News Reporter-Staff News Editor at Health & Medicine Week -- New study results on Clostridium acetobutylicum have been published. [Extracted from the article]

Keywords for this news article include: University Teknologi Malaysia, Health and Medicine, Gram-Positive Bacteria, Clostridium acetobutylicum, Gram-Positive Endospore-Forming Rods, Gram-Positive Endospore-Forming Bacteria. Clostridium, Clostridium acetobutylicum, Gram-Positive Bacteria, Gram-Positive Endospore-Forming Bacteria, Gram-Positive Endospore-Forming Rods, Health and Medicine Keywords: Clostridium; Clostridium acetobutylicum; Gram-Positive Bacteria; Gram-Positive Endospore-Forming Bacteria; Gram-Positive Endospore-Forming Rods; Health and Medicine EN Clostridium Clostridium acetobutylicum Gram-Positive Bacteria Gram-Positive Endospore-Forming Bacteria Gram-Positive Endospore-Forming Rods Health and Medicine 6578 6578 1 10/30/23 20231103 NES 231103 2023 NOV 3 (NewsRx) -- By a News Reporter-Staff News Editor at Health & Medicine Week -- Researchers detail new data in Clostridium acetobutylicum. [Extracted from the article]

Keywords: Kiev; Ukraine; Europe; Clostridium; Genetics; Gram-Positive Bacteria; Gram-Positive Endospore-Forming Bacteria; Gram-Positive Endospore-Forming Rods EN Kiev Ukraine Europe Clostridium Genetics Gram-Positive Bacteria Gram-Positive Endospore-Forming Bacteria Gram-Positive Endospore-Forming Rods 87 87 1 07/24/23 20230727 NES 230727 2023 JUL 27 (NewsRx) -- By a News Reporter-Staff News Editor at Food Weekly News -- Investigators publish new report on Gram-Positive Endospore-Forming Bacteria - Clostridium. The news reporters obtained a quote from the research from the National Academy of Sciences Ukraine, "In this study, the entire genome of the producing strain Clostridium sp. [Extracted from the article]

Keywords: Clostridium; Clostridium acetobutylicum; Enzymes and Coenzymes; Gram-Positive Bacteria; Gram-Positive Endospore-Forming Bacteria; Gram-Positive Endospore-Forming Rods; Health and Medicine; Synthetase EN Clostridium Clostridium acetobutylicum Enzymes and Coenzymes Gram-Positive Bacteria Gram-Positive Endospore-Forming Bacteria Gram-Positive Endospore-Forming Rods Health and Medicine Synthetase 753 753 1 07/10/23 20230714 NES 230714 2023 JUL 14 (NewsRx) -- By a News Reporter-Staff News Editor at Genomics & Genetics Weekly -- Fresh data on Clostridium acetobutylicum are presented in a new report. Here we show for the industrial organism Clostridium acetobutylicum that the LanM-like protein CA C0082 is not required for the production of active AgrD-derived signaling peptide but nevertheless acts as an effector of Agr quorum sensing. New Findings from University of Nottingham Describe Advances in Clostridium acetobutylicum (The Lanthipeptide Synthetase-like Protein CA C0082 Is an Effector of Agr Quorum Sensing in Clostridium acetobutylicum). [Extracted from the article]

Keywords: Life Sciences; Microorganism Research EN Life Sciences Microorganism Research 547 547 1 07/10/23 20230714 NES 230714 2023 JUL 14 (NewsRx) -- By a News Reporter-Staff News Editor at Genomics & Genetics Weekly -- Investigators discuss new findings in microorganism research. Keywords for this news article include: Indian Institute of Technology (IIT) Gandhinagar, Gujarat, India, Asia, Life Sciences, Microorganism Research. [Extracted from the article]

Alcohols, Biofuel, Biotechnology, Clostridium, Clostridium acetobutylicum, Energy, Engineering, Ethanol, Ethanolamines, Genetics, Gram-Positive Bacteria, Gram-Positive Endospore-Forming Bacteria, Gram-Positive Endospore-Forming Rods, Health and Medicine, Renewable Energy Keywords: Alcohols; Biofuel; Biotechnology; Clostridium; Clostridium acetobutylicum; Energy; Engineering; Ethanol; Ethanolamines; Genetics; Gram-Positive Bacteria; Gram-Positive Endospore-Forming Bacteria; Gram-Positive Endospore-Forming Rods; Health and Medicine; Renewable Energy EN Alcohols Biofuel Biotechnology Clostridium Clostridium acetobutylicum Energy Engineering Ethanol Ethanolamines Genetics Gram-Positive Bacteria Gram-Positive Endospore-Forming Bacteria Gram-Positive Endospore-Forming Rods Health and Medicine Renewable Energy 1603 1603 1 07/03/23 20230707 NES 230707 2023 JUL 7 (NewsRx) -- By a News Reporter-Staff News Editor at Genomics & Genetics Weekly -- Researchers detail new data in biofuel. [Extracted from the article]

Keywords: Naples; Italy; Europe; Clostridium; Clostridium acetobutylicum; Gram-Positive Bacteria; Gram-Positive Endospore-Forming Bacteria; Gram-Positive Endospore-Forming Rods; Health and Medicine EN Naples Italy Europe Clostridium Clostridium acetobutylicum Gram-Positive Bacteria Gram-Positive Endospore-Forming Bacteria Gram-Positive Endospore-Forming Rods Health and Medicine 2023 FEB 10 (NewsRx) -- By a News Reporter-Staff News Editor at Health & Medicine Week -- Current study results on Gram-Positive Bacteria - Clostridium acetobutylicum have been published. Naples, Italy, Europe, Clostridium, Clostridium acetobutylicum, Gram-Positive Bacteria, Gram-Positive Endospore-Forming Bacteria, Gram-Positive Endospore-Forming Rods, Health and Medicine. [Extracted from the article]

Keywords: Clostridium; Clostridium acetobutylicum; Gram-Positive Bacteria; Gram-Positive Endospore-Forming Bacteria; Gram-Positive Endospore-Forming Rods; Health and Medicine; Mutagenesis EN Clostridium Clostridium acetobutylicum Gram-Positive Bacteria Gram-Positive Endospore-Forming Bacteria Gram-Positive Endospore-Forming Rods Health and Medicine Mutagenesis 2023 FEB 10 (NewsRx) -- By a News Reporter-Staff News Editor at Genomics & Genetics Weekly -- Investigators publish new report on Clostridium acetobutylicum. Clostridium, Clostridium acetobutylicum, Gram-Positive Bacteria, Gram-Positive Endospore-Forming Bacteria, Gram-Positive Endospore-Forming Rods, Health and Medicine, Mutagenesis. [Extracted from the article]

In continuous phosphate-limited conditions, under pH control from high pH (pH ≳ 5.2) to low pH (pH ≲ 5.2), the metabolism of the Gram-positive bacterium Clostridium acetobutylicum, switches from acid to solvent production. Three main enzymes are responsible for the shift, acetoacetate decarboxylase (Adc), alcohol dehydrogenase (AdhE1/2) and a CoA-transferase (CtfA/B), which are produced in increased quantities during solventogenesis. A two-population model, Millat et al. (2013) and fitted to such ‘forward’-shift data, can explain this, as well as observed changes in optical density immediately following the shift: an acidogenic subpopulation is washed out and a solventogenic subpopulation grows in its place, each with distinct physiologies and proteomes. We fit this model to a ‘reverse’-shift experiment, where the pH is increased from solventogenic to acidogenic conditions. We find corresponding changes in reaction rates, with AdhE1 and Adc production falling, as in the ‘forward’ experiments; however, for CtfA/B, the best fit surprisingly arises from the same level of production in both conditions. We propose experiments that would test whether this is a model artefact or accurately reflects cultures shifted in this reverse direction, and, if true, may suggest that over-expressing CtfA/B in both solventogenic and acidogenic conditions could improve the efficiency of fermentation. [ABSTRACT FROM AUTHOR]

The Gram-positive bacterium Clostridium acetobutylicum is an anaerobic endospore-forming species which produces acetone, butanol and ethanol via the acetone–butanol (AB) fermentation process, leading to biofuels including butanol. In previous work we looked to estimate the parameters in an ordinary differential equation model of the glucose metabolism network using data from pH-controlled continuous culture experiments. Here we combine two approaches, namely the approximate Bayesian computation via an existing sequential Monte Carlo (ABC-SMC) method (to compute credible intervals for the parameters), and the profile likelihood estimation (PLE) (to improve the calculation of confidence intervals for the same parameters), the parameters in both cases being derived from experimental data from forward shift experiments. We also apply the ABC-SMC method to investigate which of the models introduced previously (one non-sporulation and four sporulation models) have the greatest strength of evidence. We find that the joint approximate posterior distribution of the parameters determines the same parameters as previously, including all of the basal and increased enzyme production rates and enzyme reaction activity parameters, as well as the Michaelis–Menten kinetic parameters for glucose ingestion, while other parameters are not as well-determined, particularly those connected with the internal metabolites acetyl-CoA, acetoacetyl-CoA and butyryl-CoA. We also find that the approximate posterior is strongly non-Gaussian, indicating that our previous assumption of elliptical contours of the distribution is not valid, which has the effect of reducing the numbers of pairs of parameters that are (linearly) correlated with each other. Calculations of confidence intervals using the PLE method back this up. Finally, we find that all five of our models are equally likely, given the data available at present. [ABSTRACT FROM AUTHOR]

His cultures of otorrhoea were positive for I Staphylococcus epidermidis i and I Clostridium hastiforme. i The worsening of acute otitis media (caused by Staphylococcus epidermidis and Clostridium hastiforme) was considered related to methylprednisolone. B Author Information b An event is serious (based on the ICH definition) when the patient outcome is: * death * life-threatening * hospitalisation * disability * congenital anomaly * other medically important event A 47-year-old man experienced worsening of acute otitis media (caused by I Staphylococcus epidermidis i and I Clostridium hastiforme i ) during treatment with methylprednisolone for acute otitis media. [Extracted from the article]

Highlights: [•] We model quorum sensing, solventogenesis and sporulation in Clostridium acetobutylicum. [•] A high population density can induce both survival processes or sporulation alone. [•] We compare the agr systems of C. acetobutylicum and Staphylococcus aureus. [•] The clostridial agr circuitry may moderate the number of spores or save energy. [Copyright &y& Elsevier]

Abstract: The acetone–butanol (AB) fermentation process in the anaerobic endospore-forming Gram-positive bacterium Clostridium acetobutylicum is useful as a producer of biofuels, particularly butanol. Recent work has concentrated on trying to improve the efficiency of the fermentation method, either through changes in the environmental conditions or by modifying the genome to selectively favour the production of one particular solvent over others. Fermentation of glucose by C. acetobutylicum occurs in two stages: initially the acids acetate and butyrate are produced and excreted and then, as the external pH falls, acetate and butyrate are ingested and further metabolised into the solvents acetone, butanol and ethanol. In order to optimise butanol production, it is important to understand how pH affects the enzyme-controlled reactions in the metabolism process. We adapt an ordinary differential equation model of the metabolic network with regulation at the genetic level for the required enzymes; parametrising the model using experimental data generated from continuous culture, we improve on previous point predictions (S. Haus, S. Jabbari, T. Millat, H. Janssen, R.-J. Fisher, H. Bahl, J. R. King, O. Wolkenhauer, A systems biology approach to investigate the effect of pH-induced gene regulation on solvent production by Clostridium acetobutylicum in continuous culture, BMC Systems Biology 5 (2011)) [1] both by using a different optimisation approach and by computing confidence intervals and correlation coefficients. We find in particular that the parameters are ill-determined from the data and that two separate clusters of parameters appear correlated, reflecting the importance of two metabolic intermediates. We extend the model further to include another aspect of the clostridial survival mechanism, sporulation, and by computation of the Akaike Information Criterion values find that the there is some evidence for the presence of sporulation during the shift. [Copyright &y& Elsevier]

Reports on the genetic engineering of the bacterium Clostridium acetobutycum to produce an enzyme that activates a cancer drug. Research from Stanford University; Innate ability to proliferate in solid tumors; Transformation of the cancer drug CB 1954 from a relatively benign state into a potent killer of cells; Experiments.

The article reports on the application of genome shuffling to enhance the production of acetone-butanol-ethanol (ABE) in Clostridium acetobutylicum CICC 8012.