Study Findings from University of Toulouse Broaden Understanding of Gene Editing (Improvement of the Genome Editing Tools Based on 5FC/5FU Counter Selection in Clostridium acetobutylicum).

A recent study conducted by researchers at the University of Toulouse in France has made advancements in the field of gene editing. The study focused on improving the genome editing tools used in Clostridium acetobutylicum, a type of bacteria. The researchers developed a method that allows for genome modification without leaving any markers or scars in the bacteria strain. They also addressed the issue of the strain becoming resistant to the selection method used, 5-fluorouracil (5FU), by creating a corrective replicative plasmid. Additionally, the researchers optimized the system by reducing the concentration of 5FU and successfully replaced a gene in the bacteria. This research has implications for biotechnology and genetics. [Extracted from the article]