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BackgroundSickle cell disease (SCD) is a life-limiting inherited hemoglobinopathy that results in significant complications and affects quality of life. Hematopoietic stem cell transplantation (HSCT) is currently the only curative intervention for SCD; however, guidelines are needed to inform how to apply HSCT in clinical practice.ObjectiveThese evidence-based guidelines of the American Society of Hematology (ASH) are intended to support patients, clinicians, and health professionals in their decisions about HSCT for SCD.MethodsThe multidisciplinary guideline panel formed by ASH included 2 patient representatives and was balanced to minimize potential bias from conflicts of interest. The Mayo Evidence-Based Practice Research Program supported the guideline development process, including performing systematic evidence reviews (through 2019). The panel prioritized clinical questions and outcomes according to their importance for clinicians and patients. The panel used the Grading of Recommendations Assessment, Development and Evaluation (GRADE) approach, including GRADE Evidence-to-Decision frameworks, to assess evidence and make recommendations, which were subject to public comment.ResultsThe panel agreed on 8 recommendations to help patients and providers assess how individuals with SCD should consider the timing and type of HSCT.ConclusionsThe evidence review yielded no randomized controlled clinical trials for HSCT in SCD; therefore, all recommendations are based on very low certainty in the evidence. Key recommendations include considering HSCT for those with neurologic injury or recurrent acute chest syndrome at an early age and to improve nonmyeloablative regimens. Future research should include the development of a robust SCD registry to serve as a comparator for HSCT studies.

Ability to accurately attribute adverse events post–gene therapy is required to describe the benefit-risk of these novel treatments. A SCD patient developed myelodysplastic syndrome post-LentiGlobin treatment; we show how insertional oncogenesis was excluded as the cause.

The hematopoietic system seeds the CNS with microglial progenitor cells during the fetal period, but the subsequent cell generation dynamics and maintenance of this population have been poorly understood. We report that microglia, unlike most other hematopoietic lineages, renew slowly at a median rate of 28% per year, and some microglia last for more than two decades. Furthermore, we find no evidence for the existence of a substantial population of quiescent long-lived cells, meaning that the microglia population in the human brain is sustained by continuous slow turnover throughout adult life., The Human Regeneration Map

The hematopoietic system seeds the CNS with microglial progenitor cells during the fetal period, but the subsequent cell generation dynamics and maintenance of this population have been poorly understood. We report that microglia, unlike most other hematopoietic lineages, renew slowly at a median rate of 28% per year, and some microglia last for more than two decades. Furthermore, we find no evidence for the existence of a substantial population of quiescent long-lived cells, meaning that the microglia population in the human brain is sustained by continuous slow turnover throughout adult life., The Human Regeneration Map

The hematopoietic system seeds the CNS with microglial progenitor cells during the fetal period, but the subsequent cell generation dynamics and maintenance of this population have been poorly understood. We report that microglia, unlike most other hematopoietic lineages, renew slowly at a median rate of 28% per year, and some microglia last for more than two decades. Furthermore, we find no evidence for the existence of a substantial population of quiescent long-lived cells, meaning that the microglia population in the human brain is sustained by continuous slow turnover throughout adult life., The Human Regeneration Map

BackgroundHaemopoietic progenitor cell (HPC) transplantation can cure sickle cell disease. Non-myeloablative conditioning typically results in donor-derived erythrocytes and stable mixed chimerism of recipient-derived and donor-derived leucocytes. Exposure to donor antigens from the HPC graft and new red cell antibodies induced by transfusion can lead to immunohaematological complications. We assessed the incidence of such complications among HPC transplant recipients with sickle cell disease.MethodsThe study population was all patients with sickle cell disease enrolled before March 31, 2015, in the three clinical trials of non-myeloablative HPC transplantation at the National Institutes of Health. We assessed formation of new red cell antibodies after transplantation and red cell incompatibility between donors and recipients.Findings61 patients were enrolled, 42 were HLA matched and 19 were haploidentical. Nine (15%) had immunohaematological complications. Before HPC transplantation, three patients had antibodies incompatible with their donors. After HPC transplantation, new red cell antibodies were seen in six patients (11 alloantibodies and two autoantibodies), among whom three developed antibodies incompatible with donor or recipient red cells and three developed compatible antibodies. The clinical course of complications was highly variable, from no severe effects attributable to antibodies, to sustained reticulocytopenia, to near-fatal haemolysis. We found no significant correlation between immunohaematological complications and graft failure, graft rejection, or death.InterpretationClinical effects ranged from seemingly not clinically important to potentially fatal. In patients with sickle cell disease, donor and recipient red cell phenotypes should be carefully assessed before transplantation to minimise and manage the risk of immunohaematological complications.FundingIntramural Research Program and National Institutes of Health.

Mixed donor chimerism after hematopoietic cell transplantation for sickle cell disease (SCD) can result in resolution of disease symptoms, but symptoms recur when donor chimerism is critically low. The relationship between chimerism, hemoglobin S (HbS) level, and symptomatic disease was correlated retrospectively in 95 patients who had chimerism reports available at day 100 and at 1 and 2 years after transplantation. Recurrent disease was defined as recurrence of vaso-occlusive crises, acute chest syndrome, stroke, and/or HbS levels > 50%. Thirty-five patients maintained full donor chimerism (myeloid or whole blood) through 2 years. Donor chimerism was less than 10% (defined as graft failure) in 13 patients during this period. Mixed chimerism was reported in the remaining 47 patients (range, 10% to 94%). The lowest documented donor chimerism without symptomatic disease was 26%. Of 12 surviving patients with recurrent disease, 2 had recurrence of symptoms before documented graft failure (donor chimerism of 11% and 17%, respectively). Three patients underwent second transplantation for graft failure. None received donor leukocyte infusion to maintain mixed chimerism or prevent graft failure. We conclude stable donor chimerism greater than 25% is associated with resolution of SCD-related symptoms, and HbS levels in transplant recipients should be interpreted in context of the sickle trait status of the donors.

The hematopoietic system seeds the CNS with microglial progenitor cells during the fetal period, but the subsequent cell generation dynamics and maintenance of this population have been poorly understood. We report that microglia, unlike most other hematopoietic lineages, renew slowly at a median rate of 28% per year, and some microglia last for more than two decades. Furthermore, we find no evidence for the existence of a substantial population of quiescent long-lived cells, meaning that the microglia population in the human brain is sustained by continuous slow turnover throughout adult life., The Human Regeneration Map

The hematopoietic system seeds the CNS with microglial progenitor cells during the fetal period, but the subsequent cell generation dynamics and maintenance of this population have been poorly understood. We report that microglia, unlike most other hematopoietic lineages, renew slowly at a median rate of 28% per year, and some microglia last for more than two decades. Furthermore, we find no evidence for the existence of a substantial population of quiescent long-lived cells, meaning that the microglia population in the human brain is sustained by continuous slow turnover throughout adult life., The Human Regeneration Map

Neurons are added throughout life in the hippocampus and olfactory bulb in most mammals, although humans represent an exception without detectable olfactory bulb neurogenesis. Nevertheless, neuroblasts are generated in the lateral ventricle wall in humans, the neurogenic niche for olfactory bulb neurons in other mammals. We show that, in humans, new neurons integrate adjacent to this neurogenic niche, in the striatum. The neuronal turnover in the striatum appears restricted to interneurons and we show that postnatally generated striatal neurons are preferentially depleted in Huntington’s disease. This demonstrates a unique pattern of neurogenesis in the adult human brain., Linnéprojekt. The Human Regenerative Map

The myelination of axons by oligodendrocytes has been suggested to be modulated by experience, which could mediate neural plasticity by optimizing the performance of the circuitry. We have assessed the dynamics of oligodendrocyte generation and myelination in the human brain. The number of oligodendrocytes in the corpus callosum is established in childhood and remains stable after that. Analysis of the integration of nuclear bomb test-derived 14C revealed that myelin is exchanged at a high rate, whereas the oligodendrocyte population in white matter is remarkably stable in humans, with an annual exchange of 1/300 oligodendrocytes. We conclude that oligodendrocyte turnover contributes minimally to myelin remodeling in human white matter and that this instead may be carried out by mature oligodendrocytes, which may facilitate rapid neural plasticity., The Human Regenerative Map, Linné Project 2008-2018

Neurons are added throughout life in the hippocampus and olfactory bulb in most mammals, although humans represent an exception without detectable olfactory bulb neurogenesis. Nevertheless, neuroblasts are generated in the lateral ventricle wall in humans, the neurogenic niche for olfactory bulb neurons in other mammals. We show that, in humans, new neurons integrate adjacent to this neurogenic niche, in the striatum. The neuronal turnover in the striatum appears restricted to interneurons and we show that postnatally generated striatal neurons are preferentially depleted in Huntington’s disease. This demonstrates a unique pattern of neurogenesis in the adult human brain., Linnéprojekt. The Human Regenerative Map

The myelination of axons by oligodendrocytes has been suggested to be modulated by experience, which could mediate neural plasticity by optimizing the performance of the circuitry. We have assessed the dynamics of oligodendrocyte generation and myelination in the human brain. The number of oligodendrocytes in the corpus callosum is established in childhood and remains stable after that. Analysis of the integration of nuclear bomb test-derived 14C revealed that myelin is exchanged at a high rate, whereas the oligodendrocyte population in white matter is remarkably stable in humans, with an annual exchange of 1/300 oligodendrocytes. We conclude that oligodendrocyte turnover contributes minimally to myelin remodeling in human white matter and that this instead may be carried out by mature oligodendrocytes, which may facilitate rapid neural plasticity., The Human Regenerative Map, Linné Project 2008-2018

Neurons are added throughout life in the hippocampus and olfactory bulb in most mammals, although humans represent an exception without detectable olfactory bulb neurogenesis. Nevertheless, neuroblasts are generated in the lateral ventricle wall in humans, the neurogenic niche for olfactory bulb neurons in other mammals. We show that, in humans, new neurons integrate adjacent to this neurogenic niche, in the striatum. The neuronal turnover in the striatum appears restricted to interneurons and we show that postnatally generated striatal neurons are preferentially depleted in Huntington’s disease. This demonstrates a unique pattern of neurogenesis in the adult human brain., Linnéprojekt. The Human Regenerative Map

The myelination of axons by oligodendrocytes has been suggested to be modulated by experience, which could mediate neural plasticity by optimizing the performance of the circuitry. We have assessed the dynamics of oligodendrocyte generation and myelination in the human brain. The number of oligodendrocytes in the corpus callosum is established in childhood and remains stable after that. Analysis of the integration of nuclear bomb test-derived 14C revealed that myelin is exchanged at a high rate, whereas the oligodendrocyte population in white matter is remarkably stable in humans, with an annual exchange of 1/300 oligodendrocytes. We conclude that oligodendrocyte turnover contributes minimally to myelin remodeling in human white matter and that this instead may be carried out by mature oligodendrocytes, which may facilitate rapid neural plasticity., The Human Regenerative Map, Linné Project 2008-2018

The occurrence of clonal perturbations and leukemia in patients transplanted with gamma-retroviral (RV) vector-transduced autologous hematopoietic stem and progenitor cells (HSPCs) has stimulated extensive investigation, demonstrating that proviral insertions may perturb adjacent proto-oncogene expression. Although enhancer-deleted lentiviruses are less likely to result in insertional oncogenesis, there is evidence that they may perturb transcript splicing, and one patient with a benign clonal expansion of lentivirally transduced HPSC has been reported. The rhesus macaque model provides an opportunity for informative long-term analysis to ask whether transduction impacts on long-term HSPC properties. We used two techniques to examine whether lentivirally transduced HSPCs from eight rhesus macaques transplanted 1-13.5 years previously are perturbed at a population level, comparing telomere length as a measure of replicative history and gene expression profile of vector positive versus vector negative cells. There were no differences in telomere lengths between sorted GFP+ and GFP- blood cells, suggesting that lentiviral (LV) transduction did not globally disrupt replicative patterns. Bone marrow GFP+ and GF- CD34+ cells showed no differences in gene expression using unsupervised and principal component analysis. These studies did not uncover any global long-term perturbation of proliferation, differentiation, or other important functional parameters of transduced HSPCs in the rhesus macaque model.

The occurrence of clonal perturbations and leukemia in patients transplanted with gamma-retroviral (RV) vector-transduced autologous hematopoietic stem and progenitor cells (HSPCs) has stimulated extensive investigation, demonstrating that proviral insertions may perturb adjacent proto-oncogene expression. Although enhancer-deleted lentiviruses are less likely to result in insertional oncogenesis, there is evidence that they may perturb transcript splicing, and one patient with a benign clonal expansion of lentivirally transduced HPSC has been reported. The rhesus macaque model provides an opportunity for informative long-term analysis to ask whether transduction impacts on long-term HSPC properties. We used two techniques to examine whether lentivirally transduced HSPCs from eight rhesus macaques transplanted 1-13.5 years previously are perturbed at a population level, comparing telomere length as a measure of replicative history and gene expression profile of vector positive versus vector negative cells. There were no differences in telomere lengths between sorted GFP+ and GFP- blood cells, suggesting that lentiviral (LV) transduction did not globally disrupt replicative patterns. Bone marrow GFP+ and GF- CD34+ cells showed no differences in gene expression using unsupervised and principal component analysis. These studies did not uncover any global long-term perturbation of proliferation, differentiation, or other important functional parameters of transduced HSPCs in the rhesus macaque model.

Neurons are added throughout life in the hippocampus and olfactory bulb in most mammals, although humans represent an exception without detectable olfactory bulb neurogenesis. Nevertheless, neuroblasts are generated in the lateral ventricle wall in humans, the neurogenic niche for olfactory bulb neurons in other mammals. We show that, in humans, new neurons integrate adjacent to this neurogenic niche, in the striatum. The neuronal turnover in the striatum appears restricted to interneurons and we show that postnatally generated striatal neurons are preferentially depleted in Huntington’s disease. This demonstrates a unique pattern of neurogenesis in the adult human brain., Linnéprojekt. The Human Regenerative Map

The myelination of axons by oligodendrocytes has been suggested to be modulated by experience, which could mediate neural plasticity by optimizing the performance of the circuitry. We have assessed the dynamics of oligodendrocyte generation and myelination in the human brain. The number of oligodendrocytes in the corpus callosum is established in childhood and remains stable after that. Analysis of the integration of nuclear bomb test-derived 14C revealed that myelin is exchanged at a high rate, whereas the oligodendrocyte population in white matter is remarkably stable in humans, with an annual exchange of 1/300 oligodendrocytes. We conclude that oligodendrocyte turnover contributes minimally to myelin remodeling in human white matter and that this instead may be carried out by mature oligodendrocytes, which may facilitate rapid neural plasticity., The Human Regenerative Map, Linné Project 2008-2018

Neurons are added throughout life in the hippocampus and olfactory bulb in most mammals, although humans represent an exception without detectable olfactory bulb neurogenesis. Nevertheless, neuroblasts are generated in the lateral ventricle wall in humans, the neurogenic niche for olfactory bulb neurons in other mammals. We show that, in humans, new neurons integrate adjacent to this neurogenic niche, in the striatum. The neuronal turnover in the striatum appears restricted to interneurons and we show that postnatally generated striatal neurons are preferentially depleted in Huntington’s disease. This demonstrates a unique pattern of neurogenesis in the adult human brain., Linnéprojekt. The Human Regenerative Map

The myelination of axons by oligodendrocytes has been suggested to be modulated by experience, which could mediate neural plasticity by optimizing the performance of the circuitry. We have assessed the dynamics of oligodendrocyte generation and myelination in the human brain. The number of oligodendrocytes in the corpus callosum is established in childhood and remains stable after that. Analysis of the integration of nuclear bomb test-derived 14C revealed that myelin is exchanged at a high rate, whereas the oligodendrocyte population in white matter is remarkably stable in humans, with an annual exchange of 1/300 oligodendrocytes. We conclude that oligodendrocyte turnover contributes minimally to myelin remodeling in human white matter and that this instead may be carried out by mature oligodendrocytes, which may facilitate rapid neural plasticity., The Human Regenerative Map, Linné Project 2008-2018

The contribution of this paper is an experimentally verified real-time algorithm for combined probabilistic search and track using multiple unmanned aerial vehicles (UAVs). Distributed data fusion provides a framework for multiple sensors to search for a target and accurately estimate its position. Vision based sensing is employed, using fixed downward-looking cameras. These sensors are modeled to include vehicle state uncertainty and produce an estimate update regardless of whether the target is detected in the frame or not. This allows for a single framework for searching or tracking, and requires non-linear representations of the target position probability density function (PDF) and the sensor model. While a grid-based system for Bayesian estimation was used for the flight demonstrations, the use of a particle filter solution has also been examined. Multi-aircraft flight experiments demonstrate vision-based localization of a stationary target with estimated error covariance on the order of meters. This capability for real-time distributed estimation will be a necessary component for future research in information-theoretic control.

The contribution of this paper is an experimentally verified real-time algorithm for combined probabilistic search and track using multiple unmanned aerial vehicles (UAVs). Distributed data fusion provides a framework for multiple sensors to search for a target and accurately estimate its position. Vision based sensing is employed, using fixed downward-looking cameras. These sensors are modeled to include vehicle state uncertainty and produce an estimate update regardless of whether the target is detected in the frame or not. This allows for a single framework for searching or tracking, and requires non-linear representations of the target position probability density function (PDF) and the sensor model. While a grid-based system for Bayesian estimation was used for the flight demonstrations, the use of a particle filter solution has also been examined. Multi-aircraft flight experiments demonstrate vision-based localization of a stationary target with estimated error covariance on the order of meters. This capability for real-time distributed estimation will be a necessary component for future research in information-theoretic control.

The contribution of this paper is an experimentally verified real-time algorithm for combined probabilistic search and track using multiple unmanned aerial vehicles (UAVs). Distributed data fusion provides a framework for multiple sensors to search for a target and accurately estimate its position. Vision based sensing is employed, using fixed downward-looking cameras. These sensors are modeled to include vehicle state uncertainty and produce an estimate update regardless of whether the target is detected in the frame or not. This allows for a single framework for searching or tracking, and requires non-linear representations of the target position probability density function (PDF) and the sensor model. While a grid-based system for Bayesian estimation was used for the flight demonstrations, the use of a particle filter solution has also been examined. Multi-aircraft flight experiments demonstrate vision-based localization of a stationary target with estimated error covariance on the order of meters. This capability for real-time distributed estimation will be a necessary component for future research in information-theoretic control.

The contribution of this paper is an experimentally verified real-time algorithm for combined probabilistic search and track using multiple unmanned aerial vehicles (UAVs). Distributed data fusion provides a framework for multiple sensors to search for a target and accurately estimate its position. Vision based sensing is employed, using fixed downward-looking cameras. These sensors are modeled to include vehicle state uncertainty and produce an estimate update regardless of whether the target is detected in the frame or not. This allows for a single framework for searching or tracking, and requires non-linear representations of the target position probability density function (PDF) and the sensor model. While a grid-based system for Bayesian estimation was used for the flight demonstrations, the use of a particle filter solution has also been examined. Multi-aircraft flight experiments demonstrate vision-based localization of a stationary target with estimated error covariance on the order of meters. This capability for real-time distributed estimation will be a necessary component for future research in information-theoretic control.

The contribution of this paper is an experimentally verified real-time algorithm for combined probabilistic search and track using multiple unmanned aerial vehicles (UAVs). Distributed data fusion provides a framework for multiple sensors to search for a target and accurately estimate its position. Vision based sensing is employed, using fixed downward-looking cameras. These sensors are modeled to include vehicle state uncertainty and produce an estimate update regardless of whether the target is detected in the frame or not. This allows for a single framework for searching or tracking, and requires non-linear representations of the target position probability density function (PDF) and the sensor model. While a grid-based system for Bayesian estimation was used for the flight demonstrations, the use of a particle filter solution has also been examined. Multi-aircraft flight experiments demonstrate vision-based localization of a stationary target with estimated error covariance on the order of meters. This capability for real-time distributed estimation will be a necessary component for future research in information-theoretic control.

The contribution of this paper is an experimentally verified real-time algorithm for combined probabilistic search and track using multiple unmanned aerial vehicles (UAVs). Distributed data fusion provides a framework for multiple sensors to search for a target and accurately estimate its position. Vision based sensing is employed, using fixed downward-looking cameras. These sensors are modeled to include vehicle state uncertainty and produce an estimate update regardless of whether the target is detected in the frame or not. This allows for a single framework for searching or tracking, and requires non-linear representations of the target position probability density function (PDF) and the sensor model. While a grid-based system for Bayesian estimation was used for the flight demonstrations, the use of a particle filter solution has also been examined. Multi-aircraft flight experiments demonstrate vision-based localization of a stationary target with estimated error covariance on the order of meters. This capability for real-time distributed estimation will be a necessary component for future research in information-theoretic control.

The contribution of this paper is an experimentally verified real-time algorithm for combined probabilistic search and track using multiple unmanned aerial vehicles (UAVs). Distributed data fusion provides a framework for multiple sensors to search for a target and accurately estimate its position. Vision based sensing is employed, using fixed downward-looking cameras. These sensors are modeled to include vehicle state uncertainty and produce an estimate update regardless of whether the target is detected in the frame or not. This allows for a single framework for searching or tracking, and requires non-linear representations of the target position probability density function (PDF) and the sensor model. While a grid-based system for Bayesian estimation was used for the flight demonstrations, the use of a particle filter solution has also been examined. Multi-aircraft flight experiments demonstrate vision-based localization of a stationary target with estimated error covariance on the order of meters. This capability for real-time distributed estimation will be a necessary component for future research in information-theoretic control.

The contribution of this paper is an experimentally verified real-time algorithm for combined probabilistic search and track using multiple unmanned aerial vehicles (UAVs). Distributed data fusion provides a framework for multiple sensors to search for a target and accurately estimate its position. Vision based sensing is employed, using fixed downward-looking cameras. These sensors are modeled to include vehicle state uncertainty and produce an estimate update regardless of whether the target is detected in the frame or not. This allows for a single framework for searching or tracking, and requires non-linear representations of the target position probability density function (PDF) and the sensor model. While a grid-based system for Bayesian estimation was used for the flight demonstrations, the use of a particle filter solution has also been examined. Multi-aircraft flight experiments demonstrate vision-based localization of a stationary target with estimated error covariance on the order of meters. This capability for real-time distributed estimation will be a necessary component for future research in information-theoretic control.

The contribution of this paper is an experimentally verified real-time algorithm for combined probabilistic search and track using multiple unmanned aerial vehicles (UAVs). Distributed data fusion provides a framework for multiple sensors to search for a target and accurately estimate its position. Vision based sensing is employed, using fixed downward-looking cameras. These sensors are modeled to include vehicle state uncertainty and produce an estimate update regardless of whether the target is detected in the frame or not. This allows for a single framework for searching or tracking, and requires non-linear representations of the target position probability density function (PDF) and the sensor model. While a grid-based system for Bayesian estimation was used for the flight demonstrations, the use of a particle filter solution has also been examined. Multi-aircraft flight experiments demonstrate vision-based localization of a stationary target with estimated error covariance on the order of meters. This capability for real-time distributed estimation will be a necessary component for future research in information-theoretic control.

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Pie de imprenta tomado del colofón al final de la primera parte, en verso de N₄, Sign.: A⁷, A-M⁸, N⁴ ; A-T⁸, V⁴, Después de los Preliminares comunes, cada una de las dos partes con portadilla propia, la segunda con viñeta xilográfica, Numerosos errores de foliación en la primera parte, Texto con apostillas marginales y letra gótica

Pie de imprenta tomado del colofón al final de la primera parte, en verso de N₄, Sign.: A⁷, A-M⁸, N⁴ ; A-T⁸, V⁴, Después de los Preliminares comunes, cada una de las dos partes con portadilla propia, la segunda con viñeta xilográfica, Numerosos errores de foliación en la primera parte, Texto con apostillas marginales y letra gótica

Theater program of "Ballet Ballads" by Jerome Moross, John Latouche, J. Harl Brown, Kenward Elmslie, and directed by Benno D. Frank. This performance opened on September 28, 1951. "Susanna and the Elders" performers: Leon Berry/Thomas Massey/John Tisdale as Preacher, Carolyn Clayton/Gail Crawford as Dancing Susanna, Margaret Constantine as Singing Susanna, Anthony Basse as Moe, An Elder, Herb Greggs as Joe, An Elder, Helen Volinsky as Juniper Tree, Vicky Lynch as Cedar Tree, Jean West as Wall, Anna Bell as Wall, and Marcia Woolley as Angel. "Song of Solomon" performers: Salvatore Collura/Samuel McCurry as Solomon, Shirley Abrams/Louise Bell/Emma Coleman as Leader, Clarence Price/Thomas Massey as Officer, Joanne D'Allesandro/ Dorothy De Vaughan as Lady with the Baby, Shirley Abrams/Louise Bell as Other Lady, Anthony Basse, Carolyn Clayton, and Herb Greggs as Dancers. "Willie the Weeper" performers: John Greenwood/Ralph Karam as Singing Willie, Floyd Gaffney/Louis Naylor/William Wingfield as Dancing Willie, Coretta Green as Singing Lil, Virginia Grady/Lois Peters/Maxine Starling as Dancing Lil. The Karamu Dancers: Anna Bell, Gayle Crawford, Hariette Eakins, Floyd Gaffney, Virginia Grady, Vicky Lynch, Pearl Johnson, Louis Naylor, Lois Peters, Maxine Starling, Marcia Woolley, William Wingfield.

Theater program of "The Mikado" by W.S. Gilbert, music by Arthur Sullivan. This performance opened on December 5, 1950. Performers: Charles King as The Mikado of Japan, William Banks as Nanki-Poo, Sherman Sneed as Ko-Ko, Joseph Roberson as Pooh-Ba, Henry Finney as Pish-Tush, Elizabeth Foster as Yum-Yum, Georgia Hill as Pitti-Sing, Corita Coleman as Peep-Bo, Shirley Abrams as Katisha. Ladies of the Ensemble: Annie Allen, Bertice Armour, Ruby Brown, Dolores Clark, Jamesina Clark, Emma Coleman, Dorothy Ellington, Elaine Gibbs, Hallie Jenkins, Allie King, Esther Overstreet, Joan Richards, Regina St. Paul, Juanita Swarms, Dorothy Wall, and Annie Wimes. Gentlemen of the Ensemble: Charles Brown, Charles Cooper, Allan Cowser, Paul Jackson, Herbert Kerr, Anthony Martin, Arnold McReynolds, Clarence Price, John Thomas, John Tisdale. Guards of the Mikado: Moses Jenkins, William Jones, and Clarence Worthy.

Theater program of "Lost in the Stars" by Maxwell Anderson and Kurt Weill. This performance ran from March-April, 1951. Performers: Paul Jackson/Joseph Roberson as Leader, Bertice Armour as Answerer, Mary Hardy/Letha Hardy/Yvonne Spearman as Nita, Martha Spearman/Ella Apple as Grace Kumalo, Sherman Sneed/Charles King as Stephen Kumalo, Sid Cohen as Station Master, John Thomas as The Young Man, Allie King as The Woman, Paul Orgill/Richard Treger as James Jarvis, David Freeman/Arthur Beaty/Timothy Dotson as Edward Jarvis, Kenward Elmslie/Ted Burr as Arthur Jarvis, Maiden Cullen/Thomas Massey as John Kumalo, Thomas Massey as Paulus, Clarence Price as William, Charles Cooper as Jared, Louis Massey/Roy Mobley/Claude Webb/William Terrence as Alex, George Davis as Foreman, Emma Coleman as Mrs. Mkize, John Tisdale as Hlabeni, Edgar Powell/Ted Burr as Eland, Betty Barnes/Coretta Green as Linda, Paul Allen as Johannes Pafuri, Joseph Roberson as Matthew Kumalo, Herbert Greggs/Henry Pickett as Absalom Kumalo, Louise Taylor as Rose, Elizabeth Foster/Jessica Brazil/Georgia Hill as Irina, Leo Cichocki as Policeman, Regina St. Paul as White Woman, Allan Davis Jr. as White Man, Sid Cohen as Burton, Jack Wiegman/Louis B. Kraus as The Judge, and Bill Jackson as Villager.

Theater program of "Lost in the Stars" by Maxwell Anderson and Kurt Weill. This performance opened on December 19, 1951. Performers: Edward Powell/Paul Jackson/Charles Cooper/John Greenwood as Leaders, Bertrice Armour/Jean Richards as Answerers, Mary Hardy/Letha Hardy as Nita, Elaine Gibbs/Martha Spearman as Grace Kumalo, Charles King/Sherman Snead as Stephen Kumalo, Michael Gallagher as Station Master, John Thomas/Robert Robinson as The Young Man, Allie King as The Young Woman, Stanton Downs/John Hurd as James Jarvis, Frankie Schmidt/David Freeman/Arthur Beatty as Edward Jarvis, William Farmer/Kenward Elmslie as Arthur Jarvis, Larry Barclay as John Kumalo, Clarence Price as Paulus, George Davis as William, Charles Cooper as Jared, Louis Massey/Kenneth Baker as Alex, John Thomas/George Davis as Foreman, Emma Coleman as Mrs. Mkize, John Tisdale as Hlabeni, Edward Powell/Michael Gallagher as Eland, Coretta Green/Juanita Allen as Linda, George Davis as Johannes Pafuri, David Casson/Joe Roberson as Matthew Kumalo, Herbert Dana Greggs/Henry Pickett as Absalom Kumalo, John Thomas/William Jones as Servant, Jeanne West as Rose, Georgia Hill/Elizabeth Foster/Louis Bell as Irina, William Jones as Policeman, Sylvia Weitz as White Woman, John Hurd as White Man, Raoul Abdul/John Hurd as Burton, and Jerome Shine as The Judge.

Theater program of "Amahl and the Night Visitors" by Gian-Carlo Menotti, and directed by Benno D. Frank. This performance opened on March 14, 1952. Performers include Edward Anderson/Charles Masa/Robert Redus/Jerry Sien as Amahl, Margaret Crays/Sylvia Hunter/Barbara Lee as Mother, Manisee Boswell/Charles Cooper as Jasper, John Brown/John Tisdale as Melchior, George Arthur/Thomas Massey/James Earl as Balthazar, Anthony Basse as Page. Dancers: Rene Clark, Gail Crawford, Henriette Eakins, Virginia Grady, Pearl Johnson, H. William Lang, June Moxley, Louis Naylor, Lois Kenny, Maxine Starling, Harrison Grant, Betty Gray, Helen Jackson, Lois Peters, Betty Jane Thrasher. Pianists: Charlotte Blank, J. Harold Brown, Eugene Sabo, and Gordon Ryan.