1. MiR-214–3p regulates Piezo1, lysyl oxidases and mitochondrial function in human cardiac fibroblasts.
- Author
-
Trevelyan, Christopher J., MacCannell, Amanda D.V., Stewart, Leander, Tarousa, Theodora, Taylor, Hannah A., Murray, Michael, Bageghni, Sumia A., Hemmings, Karen E., Drinkhill, Mark J., Roberts, Lee D., Smith, Andrew J., Porter, Karen E., Forbes, Karen A., and Turner, Neil A.
- Subjects
- *
LYSYL oxidase , *GENE expression , *MITOCHONDRIAL proteins , *CITRATE synthase , *NON-coding RNA - Abstract
• miR-214-3p is enriched in cardiac fibroblasts and its expression is upregulated during cardiac remodelling. • The effects of miR-214-3p overexpression on the human cardiac fibroblast proteome were evaluated by TMT proteomics analysis and in silico network analysis. • miR-214-3p overexpression decreased expression and activity of the Piezo1 mechanosensitive cation channel. • miR-214-3p overexpression increased expression of the entire lysyl oxidase (LOX) family of collagen cross-linking enzymes. • miR-214-3p overexpression decreased expression of an array of mitochondrial proteins, including mitofusin-2 (MFN2), resulting in mitochondrial dysfunction. Cardiac fibroblasts are pivotal regulators of cardiac homeostasis and are essential in the repair of the heart after myocardial infarction (MI), but their function can also become dysregulated, leading to adverse cardiac remodelling involving both fibrosis and hypertrophy. MicroRNAs (miRNAs) are noncoding RNAs that target mRNAs to prevent their translation, with specific miRNAs showing differential expression and regulation in cardiovascular disease. Here, we show that miR-214–3p is enriched in the fibroblast fraction of the murine heart, and its levels are increased with cardiac remodelling associated with heart failure, or in the acute phase after experimental MI. Tandem mass tagging proteomics and in-silico network analyses were used to explore protein targets regulated by miR-214–3p in cultured human cardiac fibroblasts from multiple donors. Overexpression of miR-214–3p by miRNA mimics resulted in decreased expression and activity of the Piezo1 mechanosensitive cation channel, increased expression of the entire lysyl oxidase (LOX) family of collagen cross-linking enzymes, and decreased expression of an array of mitochondrial proteins, including mitofusin-2 (MFN2), resulting in mitochondrial dysfunction, as measured by citrate synthase and Seahorse mitochondrial respiration assays. Collectively, our data suggest that miR-214–3p is an important regulator of cardiac fibroblast phenotypes and functions key to cardiac remodelling, and that this miRNA represents a potential therapeutic target in cardiovascular disease. [ABSTRACT FROM AUTHOR]
- Published
- 2024
- Full Text
- View/download PDF