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Copyright of Výživa a Potraviny is the property of Spolecnost pro Vyzivu and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Introduction: Dental pulp stem cells (DPSCs) express naturally high positivity for surface receptor glycoprotein CD 44 which is involved in the induction of odontoblast mineralization with hyaluronic acid (HA) being its major ligand. The aim of this experiment was to assess the effect of HA in low (LMW-HA), medium (MMW-HA) and high (HMW-HA) molecular weights on the phenotypic profile, proliferation activity and differentiation potential of human DPSCs. Methods: The experiment was conducted in vitro on two lines of human DPSCs from different donors (third molar -- male, 25 years and first premolar -- male, 9 years). These lines were cultured in standard medium and from the second passage also in three experimental culture media containing 0.1% HA in three molecular weights: LMW-HA (116 kDa), MMW-HA (540 kDa) and HMW-HA (1500 kDa). The phenotypic analysis was performed in the seventh passage using a Vi-Cell XR flow cytometer, viability was evaluated by the Vi-Cell Analyzer in the seventh passage and proliferation activity measured by the Z2 Counter Analyzer in every passage. Osteo- and chondrodifferentiation were inducted by commercially supplied cultivation media and demonstrated by histological staining with alcian blue and alizarin red. Results: DPSCs used in our experiment expressed phenotype typical for human DPSCs (high positivity for CD 13, CD 29, CD 44, CD 90 and OCT 3/4), they were able to exceed Hayflick limit and differentiate in the osteogenic as well as the chondrogenic extracellular matrix. During the experiment, DPSCs line 1 cultivated in control medium / medium 1 (116 kDa HA) / medium 2 (540 kDa HA) / medium 3 (1500 kDa HA) achieved in this order in total 14.1/15.3/15.4/14.8 population doublings. The median of doubling time with the minimal and maximal values in the same order was 31.6 (29.2; 36.8) / 29.6 (28.5; 30.6) / 30.3 (26.7; 31.3) / 30.5 (29.7; 33.8) hours. The viability of the DPSCs obtained from the seventh passage was in the same order 92.3/93.1/91.8/92.6%. DPSC line 2 cultivated in control medium / medium 1 (116 kDa HA) / medium 2 (540 kDa HA) / medium 3 (1500 kDa HA) achieved in this order in total 16.7/17.2/16.7/16.8 population doublings. The median of doubling time with the minimal and maximal values in the same order was 28.9 (24.5; 35.2) / 27.4 (24.5; 32.6) / 28.3 (24.0; 38.4) / 27.1 (25.3; 33.9) hours. The viability of the DPSCs obtained from the seventh passage was in the same order 80.1/82.5/81.8/80.9%. Conclusion: We verified that DPSCs in the presence of hyaluronic acid at a concentration of 0.1% and molecular weights of 116, 540 and 1500 kDa survive, proliferate and maintain the ability to differentiate in mature cellular elements. We also verified the original assumption that the low molecular weight form of hyaluronic acid has a different impact on the DPSCs' phenotype than the high molecular weight form of hyaluronic acid. [ABSTRACT FROM AUTHOR]

Copyright of Journal of International Relations / Medzinarodne Vztahy is the property of University of Economics in Bratislava, Faculty of International Relations and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Cell therapy has been proposed as an alternative strategy in patients with critical limb ischemia not eligible for endovascular or surgical revascularization. Several pre-clinical and clinical studies suggest that delivery of stem cells with regenerative potential and paracrine ability can improve tissue perfusion and thus prevent amputation via the induction of capillary or collateral growth in a process called "therapeutic angiogenesis". Many cell types have been tested, but results of most clinical trials to date rely on the use of adult autologous bone marrow-derived mononuclear cells or cultured peripheral blood-derived proangiogenic mononuclear cells, all typically delivered unaltered in their native state. Of increasing interest are mesenchymal stem cells with the capability for multipotent differentiation, cytoprotection, and damaged tissue restoration. Although the meta-analyses of randomized clinical trials demonstrate a positive benefit-to-risk ratio of cell-based therapy, its promising potential needs to be confirmed by larger randomized, placebo-controlled trials. This paper provides current evidence about cell therapy of limb ischemia and appraises some of the future perspectives of regenerative therapy. Fig. 3, Tab. 4, Ref. 39, Online full text (Free, PDF) www.cardiology.sk [ABSTRACT FROM AUTHOR]

We analyzed retrospectively a group of 339 unrelated donors who underwent bone marrow (n=169) or peripheral stem cell (n=170) collection in the years 1999-2009. The required doses of haematopoietic stem cells were collected in 60 % of procedures, with no significant difference between men and women. Suboptimal grafts were obtained in 2 % of bone marrow collections and 5 % of peripheral blood stem cell collections. The frequency of adverse events was 55 % (51 % of bone marrow and 59 % of peripheral blood stem cell collections). Nausea and vomiting following general anaesthesia and pain at the aspiration site were the most common reactions in bone marrow donors. Peripheral blood stem cell collections were most frequently associated with leukopenia and thrombocytopenia. A higher incidence of adverse events was observed in women. Neither severe adverse events nor donor death associated with the collection were recorded during the observed period. The study demonstrated that the collection of haematopoietic stem cells from healthy donors is a safe procedure with a low risk of complications. The donor registry has established standard procedures for donor selection, for examinations to be conducted before collection as well as for the process of caring for donors during collection. Compliance with these rules in combination with the high professionalism of all participating health care personnel decreases the probability of severe or unexpected complications during donation. [ABSTRACT FROM AUTHOR]