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Snitrosoglutathione (GSNO) reductase regulates novel endogenous S-nitrosothiol signaling pathways, and mice deficient in GSNO reductase are protected from airways hyperreactivity. S-nitrosothiols are present in the airway, and patients with cystic fibrosis (CF) tend to have low S-nitrosothiol levels that may be attributed to upregulation of GSNO reductase activity. The present study demonstrates that 1) GSNO reductase activity is increased in the cystic fibrosis bronchial epithelial (CFBE41o-) cells expressing mutant F508del-cystic fibrosis transmembrane regulator (CFTR) compared with the wild-type CFBE41o- cells, 2) GSNO reductase expression level is increased in the primary human bronchial epithelial cells expressing mutant F508del-CFTR compared with the wild-type cells, 3) GSNO reductase colocalizes with cochaperone Hsp70/Hsp90 organizing protein (Hop; Stip1) in human airway epithelial cells, 4) GSNO reductase knockdown with siRNA increases the expression and maturation of CFTR and decreases Stip1 expression in human airway epithelial cells, 5) increased levels of GSNO reductase cause a decrease in maturation of CFTR, and 6) a GSNO reductase inhibitor effectively reverses the effects of GSNO reductase on CFTR maturation. These studies provide a novel approach to define the subcellular location of the interactions between Stip1 and GSNO reductase and the role of S-nitrosothiols in these interactions. [ABSTRACT FROM AUTHOR]

In this article we establish the uniqueness of solutions to first-order matrix dynamic equations on time scales. These results extend the results presented in [16], to more complex systems of n x n matrices. Following the ideas in [5, Chap 5], we identify Lipschitz conditions that are suitable for generalizing n x n models on time scales. [ABSTRACT FROM AUTHOR]

In this article, we study the existence of solutions to first order non-linear initial value problems within the field of "dynamic equations on time scales". We employ the method of upper and lower solutions and Schauder's fixed point theorem. We also provide sufficient conditions under which the upper and lower solutions converge uniformly to a solution. Some examples are given to illustrate the new results. [ABSTRACT FROM AUTHOR]