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This study aimed to investigate the effects of auxin types and their concentrations on rooting of the rootstock cuttings and BA on grafting. First, IBA, NAA or IBA+NAA, each at 0, 1000, 2000, and 3000 ppm were used as treatments to study rooting of Piper colubrinum. In the second experiment, BA (0 and 50 ppm) was applied to P. nigrum scion clones of Natar-1 and Petaling-2, before being grafted to the rootstocks. Results showed, that all types of auxins (IBA, NAA or IBA+NAA) induced rooting and shoot growth, and the increase of auxin concentrations led to the increase of rooting. However, their effectiveness was different. NAA and IBA+NAA were superior to IBA, and at 2000 ppm, IBA+NAA resulted in the best plant growth, as indicated by higher values of both rooting and shoot growth parameters. Compared to control, BA treatment on scions resulted in higher grafting success (80% vs. 93%) for Natar-1, and (73% vs.100%) for Petaling-2 clones. BA treatment on scions also induced more calluses in the graft union and better shoot growth. To our knowledge, this is the first report on the use of BA to increase success of grafting between P. nigrum and P. colubrinum.

Health cadres play a vital role as community educators and frontline workers in the stunting handling program. Maternal knowledge regarding adequate nutrition for toddlers from pregnancy to birth is a crucial factor in preventing stunting, as it influences the mother's choices in terms of food ingredients and diversity. Pandeglang Regency is among the 100 priority cities/regencies in stunting reduction efforts. This activity aims to provide training to enhance the knowledge and capacity of Integrated Health Post (Posyandu) cadres in stunting, anthropometry, and nutrition. The trained cadres will be able to actively contribute to stunting prevention programs in the targeted stunting locus area of the Pandeglang Regency. The training methods include classroom training, Posyandu simulations, advocacy, and licensing. The exercise focuses on three stunting locus villages: Kedeumaneh, Kadeubelang, and Medong. Knowledge improvement was evaluated through pre-test and post-test assessments, followed by simulations conducted at each Posyandu location. The results indicated that 30 cadre respondents demonstrated increased knowledge after the training (p-value = 0.044). Furthermore, the simulation results revealed that the cadres were able to accurately perform anthropometric measurements.

Elderly, chronic disease, depression, health perception, dependency level, which affect the quality of life of the elderly. Various interventions are needed by the elderly to overcome the decline in mobility related to age. The purpose of this study is to identify the effectiveness of stretching exercise on the quality of life and functional status of the elderly with type 2 diabetes mellitus. test group design with one intervention group. The total sample in this study was 30 elderly with type 2 diabetes. The sample was taken using a consecutive sampling technique. The intervention given was stretching exercise for 4 weeks. The Wilcoxon Signed Ranks Test statistical test showed that there was a difference between before and after the stretching exercise intervention on the functional status of the elderly, quality of life, general health dimensions, physical health, and social with a p-value (p=0.00), but there is no relationship on the psychological health and environmental dimension. Based on the results of the study, it can be concluded that stretching exercises can improve physical fitness, muscle mass and joint flexibility which have an impact on functional status and quality of life of the elderly. It is hoped that stretching exercise can be used as one nursing independent treatment to improve the quality of life of and increasing the independence of the elderly through the provision of education and regular physical exercise.

Diuron herbicide has been used in the pineapple plantation in Lampung, Indonesia, for more than 35 years. It has been realized that the use of herbicides with the same mode of action intensively can speed up the evolution of resistant weeds over a long period of time. This study aimed to determine whether Dactyloctenium aegyptium from pineapple plantation has evolved resistance to diuron and to examine whether the resistance correlates with the weed physiological activities. The study was conducted at the University of Lampung, from September 2018 to March 2019. The study consisted of two stages, i.e. Stage 1: Weed resistance test and Stage 2: Physiological activity test on resistant weed. The study used a split-plot design. The main plot was the origins of weeds (exposed and unexposed to diuron) and thhe supplots was the diuron dose. The result showed that D. aegyptium exposed has high-level resistance to diuron. The physiological activities of D. aegyptium which has a high level of diuron resistance exhibited higher carbon assimilation, stomatal conductance, and transpiration rates than the sensitive D. aegyptium.

Home and the residential environment are one of the risk factors for stunting. The level of knowledge determines residents' behavior, and knowledge is influenced by the exposure to information that a person receives. The purpose of community service activities is counseling about healthy homes and healthy behavior to Baduta mothers (children under two years) in the stunting locus, Langensari village, Pandeglang district. Extension activities were carried out on September 20, 2019, in Langensari village, Saketi District, Pandeglang Regency. Before the activity was carried out, the team carried out advocacy and licensing at the Saketi District Health Center and the Langensari Village Head. The method used in this activity is advocacy and socialization of activities and activities using extension media using posters and brochures. The target of the counseling is the Baduta mother. Participants will be assessed whether there is increased knowledge about healthy homes and healthy behavior before and after counseling (pre-post-test). The result of the educational activity was that the extension participants consisted of 39 Baduta mothers. The difference in the mean score of the pre-post-test was 0.821, and the extension activities could increase the participants' knowledge before and after counseling (p = 0.018).

Penelitian ini bertujuan untuk mengetahui pengaruh berbagai jenis auksin terhadap beberapa konsentrasi terhadap pengakaran Piper colubrinum. Percobaan dilaksanakan di Rumah Kaca Fakultas Pertanian Universitas Lampung dari bulan Mei 2018 sampai Desember 2018. Percobaan menggunakan rancangan acak lengkap dengan 3 ulangan. perlakuan yang mengaplikasikan: kontrol, IBA 1500 ppm, IBA 2000 ppm, NAA 2000 ppm, NAA 750 ppm + IBA 750 ppm, NAA 1000 ppm + IBA 1000 ppm. Hasil penelitian menunjukkan bahwa pada variabel jumlah akar primer di buku pada perlakuan NAA 750 ppm + IBA 750 ppm nilai rata-rata meningkat yaitu 21 helai dibandingkan kontrol 2,5 helai. Pada perlakuan NAA 750 ppm + IBA 750 ppm berpengaruh pada peningkatan jumlah akar primer di penampang batang yaitu 14 dibandingkan dengan kontrol 11 helai. Demikian perlakuan NAA 750 ppm + IBA 750 ppm berpengaruh pada peningkatan jumlah akar primer yaitu 34,3 helai dibandingkan dengan kontrol 13,5 helai. Untuk variabel bobot basah akar nilai rata-rata NAA 750 ppm + IBA 750 ppm yaitu 11,8 g, nilai rata-rata ini lebih tinggi dibandingkan semua perlakuan.

Correlation of macronutrient intake with body mass index, blood sugar levels, and total blood protein in drug users Background: Random blood sugar level and total blood protein need to be measured among drug users because their macronutrient intakes (carbohydrates, fat, protein, and energy) are not appropriate to the pattern of daily habits before uses drugs. Drug users had decreased appetite during the influence and withdrawal symptoms of drugs with the impacts on their body mass index (BMI). Objective: To analyze the correlation of macronutrient intakes between the random blood sugar level, total blood protein, and BMI drug users. Methods: This research was conducted with a cross-sectional design and observational study. 73 drug users were included in the study with the screening by inclusion criteria. 24-hour food recall was used to collect the macronutrient intakes, random blood sugar levels and total blood protein were monitored by the GOD-PAP method, and BMI was measured by weight and height. Data analysis used Pearson’s correlation test in bivariate and multivariate was carried out by multiple linear regressions. Results: Pearson’s correlation analysis showed that there was a significant correlation between macronutrient intakes (energy, carbohydrate, fat) with random blood sugar level, total blood protein, and BMI. BMI was the most affected by energy (β=0.531), random blood sugar level was the most affected by carbohydrates (β=0.073), and total blood protein was the most affected by protein (β=0.837). Conclusions: Macronutrient intake is significantly related to BMI, random blood sugar levels, and total blood protein in drug users. Community collaboration with related parties such as the public health service and National Narcotics Agency will very quickly detect drug side effects early on eating disorders that will affect the nutritional status of its users.

Effectiveness of the application of organic matter and Trichoderma viride from suppresive soil to control fusarium wilt on banana plant. Fusarium wilt disease caused by Fusarium oxysporum f.sp. cubense is one of the problems in banana production. This research was aimed to evaluate the effect of Trichoderma viride isolated from suppresive soil and organic matter on controlling fusarium wilt on banana plant. The efficcacy trial consisted of 12 treatments and four replications within each treatment. Each experimental unit consisted of 10 plants. Organic matters used were rice straw, cassava peel, and compost. Treatments were arranged in a randomized complete block design. Data obtained were analyzed by analysis of variance followed by orthogonal analysis (P≤0.05). Application of T. viride suppressed disease incident up to 65%. The highest disease incident occurred in plants treated without T. viride and organic matter and in plants treated with organic matter only (78.33%). Disease incidence in plants treated with T.viride was lower than those treated with the combination of T. viride and organic matter. Different application times (in nursery or soil medium) did not significantly affect fusarium wilt incidence. The type of organic matter did not significantly affect the incidence and severity of fusarium wilt.

This research aimed to study effects of IBA, NAA and their combination on rooting and shoot sprouting in Malay apple cuttings. Cuttings from superior genotype were collected and treated with (in ppm w/w): 0, 2000 IBA, 4000 IBA, 2000 NAA, 4000 NAA, 1000 IBA+1000 NAA, 2000 IBA+2000 NAA. To record the timing and percentage of rooting, cuttings were treated with (in ppm w/w) 1000 IBA+1000 NAA or without auxin as control. The results revealed that application of auxin was significantly enhanced root formation as shown by the significant increases in rooting percentage and number of roots. NAA at 2000 or 4000 ppm was the most effective auxin to promote root formation (100 %, 17.8–25.5 roots per cuttings), followed by NAA+IBA (100 %, 16.8– 9.8 roots per cuttings) and the least effective was IBA alone (79-100 %, 3.2–7.1 roots per cutting). The best treatment for rooting and shoot sprouting were (in ppm) 1000 IBA+1000 NAA, since it produced higher root length, better root morphology and higher shoot sprouting. It was also found that a combination of IBA+NAA each at 1000 ppm not only enhanced root percentage, but also shortened the time for root formation.

Dendrobium (Orchidaceae) is one of the most popular orchid genera consisting more than 2000 species, which is widely known for its long vaselife, various, shapes and colors, high frequency of flowering per year and long and flexible stalks, so it is easily assembled for flower arrangements. The objectives of this research was to study the effects of different host trees and position of plant attachment at the tree on growth of Dendrobium seedlings. This experiment was conducted with treatments arranged factorally (3 X 2) in a completely randomized design with three replicates. The first factor was the species of host three consisted of Akasia (Acacia auriculiformis), Cemara Bundel (Cupressus retusa) and Kerai Payung (Filicium decipiens) and the second factor was position of plant attachment on the tree, which consisted of attachment at the bottom ( 0,5 m above ground ) and the upper was ( 1,5 m above ground ). Each experimental unit consisted of two Dendrobium seedlings. The results showed that in general both host trees and position of attachment did not affect number of shoots, number of leaves, plant height and the longest root length. however, different host trees and its interaction with the position of attachment affected the number of roots, resulted in the highest number of roots obtained in Kerai Payung (Fillicium decipiens) at 1,5 m above ground. Keywords: Acacia auriculiformis, Cupressus retusa, host tree, Filicium decipiens, orchid.

Forest area of Balik Bukit Resort allocated as regions of natural preservation. This area hasmany potential/natural resources that could be developed. This area is located at BukitBarisan Selatan National Park which have diverse types of flora, including various types oforchids. Approximately there were 154 typeof orchids in this area(Indonesia Institute ofSciences, 2011). This research aims were to know the population and distribution patterns ofEria spp orchids.This research was conducted in August 2015. The method used is the doubleplots systematically method with sampling intensity (SI) 0.1%. The size of each swath of 20 mx 20 m and the total area of the Balik Bukit Resort was 3,560 ha, so that the total number ofsample plots were 90 units. The observed variables were types of Orchid, Orchid populationsand patterns of spread of Eria spp. Based on the results of the study, there were 10 different types of Eria spp.. Eria iridifolia found most of 22.50 individuals/ha compared to other typesof orchids Eria. Followed by the type of E. erecta of 14.72 individuals/ha E. flavescens andtype of 8.33 individuals/ha. Whereas other types is E. discolor, E. javanica, E. junghunii, E.mucronata, E. oblitterata, E. retusa, E. robusta has a density of 1 — 4 individuals/ha.Distribution pattern of the orchids E. discolor, E. erecta, E. flavescens, E. iridifolia, E.javanica, E. mucronata, E. obletterata, and E. retusa enter into the category of a huddle.While the orchids E. junghunii belonging to the category of uniform and the orchids E.robusta into random categories. Keywords: distribution pattern, Eria spp. orchids, population

Tuberculosis (TB) is a contagious disease caused by the group of Mycobacterium bacteria, Mycobacterium tuberculosis. There are six countries with the highest number of TB incidents in the world, including Indonesia with a prevalence of 1,020 per 100,000 inhabitants located second after India. Based on the results of monitoring and evaluation of Tuberculosis External Monitoring Mission Team in 2008, the success of treatment is still low. Many factors that influence the success of tuberculosis treatment one of them is the level of knowledge. This study aims to see and prove whether there is a relationship of knowledge level to the success of treatment in patients with tuberculosis and done cross-sectional. The result of the research shows that there are 45 respondents (80,4%) and the knowledge level of the respondent has good knowledge, 22 people (39,3%) from 56 samples. From the results of statistical tests using Chi-Square test obtained P value> 0.05 is 0.069.

Tuberculosis is a disease of global concern. By 2015 six countries contributing to 60% of the global total are India, Indonesia, China, Nigeria, Pakistan and South Africa. China, India and Indonesia alone accounted for 45% of cases in the world.The World Health Organization (WHO)has recommended the Directly Observed Treatment Shortcourse (DOTS) strategy for TB control by involving Drug Supervisors (PMO). It aims to achieve patient recovery, prevent transmission, and avoid drug resistant cases. This study aims to determine the relation between the roles of treatment observers (PMO) with the success of tuberculosis treatment at community health center of Johar Baru Central Jakarta in 2016.This research was conducted by Cross-Sectional non-experimental quantitative method. Population and sample are the patient of adult pulmonary and extrapulmonary tuberculosis at community health center of Johar Baru Central Jakarta in 2016. Samples are selected by using Simple Random Sampling. The data were collected by interview using questionnaire. Data analysis using SPSS with Chi-Square test.There were 45 respondents (80,4%) succeed in TB treatment and respondent with PMO roles category were 40 (71,4%). Result of statistical test using Chi-Square test obtained P value = 1,000 (> 0,05).There is no relation between the roles of treatment observers (PMO) with the success of tuberculosis treatment at community health center of Johar Baru Central Jakarta in 2016.

The prevalence of stunting in Indonesian children under five years of age is about 20%. Chronic maternal malnutrition contributes to the risk of stunting by affecting global DNA methylation. In the present study, we aimed to evaluate the levels of 5-methyl-cytosine (5mC), as a surrogate marker of global DNA methylation, in buccal swabs and its potential association with risk of stunting and cognitive performance. The levels of 5mC were measured using the enzyme-linked immunosorbent assay. The Wechsler Preschool and Primary Scale of Intelligence was used to measure cognitive functions. Buccal swab DNA samples and anthropometric data were collected from a total of 231 children aged zero to five years. In this cross-sectional cohort, the prevalence of stunting was 37% in 138 children aged zero to two years and 30% in 93 children aged > two years. The univariable analysis revealed that the levels of 5mC in buccal swab DNA were significantly lower in severely stunted children (median, 2.84; interquartile range [IQR], 2.39-4.62; P -value, 0.0314) and in children of a younger age (median, 2.81; IQR 2.53-4.62, P- value, 0.0001) than in normal (median, 3.75; IQR, 2.80-4.74) and older children (median, 4.01, IQR, 3.39-4.87), respectively. We also found that the average cognitive scores tended to be low in boys and stunted children, although the differences were not statistically significant. Furthermore, levels of 5mC found in buccal and mouthwash DNA were not associated with cognitive scores.