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Buccal DNA global methylation and cognitive performance in stunted children under 5 years of age.

Authors :
Handoyo Utomo AR
Yusnita Y
Sari SM
Ranakusuma OI
Bagaskara S
Sari W
Suciati Y
Nur Hidayati AP
Nihayah S
Putro CA
Nurainy N
Source :
Journal of biomedical research [J Biomed Res] 2024 Aug 25, pp. 1-9. Date of Electronic Publication: 2024 Aug 25.
Publication Year :
2024
Publisher :
Ahead of Print

Abstract

The prevalence of stunting in Indonesian children under five years of age is about 20%. Chronic maternal malnutrition contributes to the risk of stunting by affecting global DNA methylation. In the present study, we aimed to evaluate the levels of 5-methyl-cytosine (5mC), as a surrogate marker of global DNA methylation, in buccal swabs and its potential association with risk of stunting and cognitive performance. The levels of 5mC were measured using the enzyme-linked immunosorbent assay. The Wechsler Preschool and Primary Scale of Intelligence was used to measure cognitive functions. Buccal swab DNA samples and anthropometric data were collected from a total of 231 children aged zero to five years. In this cross-sectional cohort, the prevalence of stunting was 37% in 138 children aged zero to two years and 30% in 93 children aged > two years. The univariable analysis revealed that the levels of 5mC in buccal swab DNA were significantly lower in severely stunted children (median, 2.84; interquartile range [IQR], 2.39-4.62; P -value, 0.0314) and in children of a younger age (median, 2.81; IQR 2.53-4.62, P- value, 0.0001) than in normal (median, 3.75; IQR, 2.80-4.74) and older children (median, 4.01, IQR, 3.39-4.87), respectively. We also found that the average cognitive scores tended to be low in boys and stunted children, although the differences were not statistically significant. Furthermore, levels of 5mC found in buccal and mouthwash DNA were not associated with cognitive scores.

Details

Language :
English
ISSN :
1674-8301
Database :
MEDLINE
Journal :
Journal of biomedical research
Publication Type :
Academic Journal
Accession number :
39148272
Full Text :
https://doi.org/10.7555/JBR.37.20230295