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Nonenzymatic and rapid monitoring of uric acid levels is of great value for early diagnosis, prevention, and management of oxidative stress–associated diseases. However, fast, convenient, and low‐cost uric acid detection remains challenging, especially in resource‐limited settings. In this study, a novel and rapid biosensing approach was developed for the simultaneous visualization and quantification of uric acid levels by using the unique surface plasmon resonance and photothermal property of 4,5‐diamino‐2‐thiouracil (DT)‐capped gold nanoparticles (AuNPs). With the presence of uric acid, DT‐capped AuNPs rapidly aggregated, and a visible color/photothermal change was used for uric acid quantification within 15 min. The limit of detection was determined to be 11.3 and 6.6 μM for the dual‐mode biosensor, leveraging the unique structure of DT to optimize reaction kinetics. Moreover, the sensor exhibited excellent anti‐interference capabilities and demonstrated potential for detecting a wide range of uric acid concentrations in complex samples, thereby reducing the need for extensive sample dilution and complex material synthesis procedures. Furthermore, validation against gold standard testing indicates that this biosensor could serve as a highly sensitive assay for quantifying uric acid levels in point‐of‐care applications, particularly in resource‐limited settings. [ABSTRACT FROM AUTHOR]

In this paper, an assessment of the natural radioactivity level, radon exhalation, metal contamination, and mineralogy of a granodiorite rock sample from Stilo, in the Calabria region, Southern Italy is presented as a case study. This rock was employed as a building material in the area under study. The specific activity of 226Ra, 232Th and 40K natural radioisotopes was assessed through high-purity germanium (HPGe) gamma-ray spectrometry. Then, several indices such as the absorbed gamma dose rate (D), the annual effective dose equivalent (AEDE), the activity concentration index (ACI) and the alpha index (Iα), were quantified to determine any potential radiological health risk related to radiation exposure from the analyzed rock. Furthermore, E-PERM electret ion chambers and inductively coupled plasma mass spectrometry (ICP-MS) measurements were carried out to properly quantify the radon exhalation rate and any possible metal pollution, respectively. In particular, to further address metal pollution factors, the geo-accumulation index (Igeo) was calculated to properly address the toxicity levels of the ecosystem originating from the detected metals. Finally, with the aim of successfully discriminating the provenance of such naturally occurring radionuclides, a combined approach involving X-ray diffraction (XRD) and µ-Raman spectroscopy was employed for the identification of the main radioisotope-bearing minerals characterizing the investigated granodiorite. The results achieved in this case study can be taken as the basis for further inquiries into background levels of radioactivity and chemical contamination in natural stone employed as building materials. [ABSTRACT FROM AUTHOR]

In this article, an investigation into the natural radioactivity content in natural inorganic pigments was carried out, together with the assessment of the radiological health risk for the public related to external exposure to ionizing radiations, via High-Purity Germanium (HPGe) γ-ray spectrometry measurements and the calculation of several indices like the absorbed γ-dose rate (D), the annual effective dose equivalent outdoor (AEDEout) and indoor (AEDEin), and the activity concentration index (I). From the obtained results, it was possible to reasonably exclude radiological hazard effects. In addition, Pearson's correlation, principal component analysis (PCA), and hierarchical cluster analysis (HCA) were carried out with the aim of determining correlations between natural radioactivity content and radiological indices and with the analyzed samples. As a result, five clusters of the investigated pigments were recognized at the highest level of detail based on their chemical composition and mineralogical nature. [ABSTRACT FROM AUTHOR]

In this study a multi-technique analysis was performed on the decorated surfaces of four ancient pottery fragments dated back XIX century A.D. withdrawn from the archaeological site of the medieval Agsu town, in Azerbaijan. During the last decade, the site underwent to an extensively archaeometric investigation by means of different non-destructive, or micro-destructive, techniques. In this work we focused our attention on the characterization of the pigmenting agents and glazes at different spatial scales from elemental to microscopic domain by using portable and not-portable equipments. In particular, the elemental and molecular compositions were successfully determined by X-ray fluorescence (XRF) and Raman spectroscopy, respectively. On one side, data deriving from portable instrument were compared with those previously obtained from not-portable approach, in view of future in situ investigations. On the other side, the overall obtained results appear crucial for the reconstruction of the production technology used by craftsman of the past.

In this paper, the evaluation of the Rn-222 radioactivity content in drinking water samples from the Calabria region, southern Italy, is reported as a case study. The Rn-222-specific activity in the analyzed groundwater samples for human use was evaluated by using the PerkinElmer Tricarb 4910 TR setup and compared with the parameter value (100 Bq L−1) reported in the reference Italian legislation, i.e., D.Lgs. 28/2016, derived from the European Directive 2013/51/Euratom. The radiological health risk for the population of the investigated area, due to the ingestion and inhalation of Rn-222 dissolved in water, was then evaluated by calculating the total annual effective dose, only in those cases where the parameter value was exceeded. The obtained results represent a main reference for the investigated area and are useful for determining any possible radiological health risk for human beings related to the ingestion of the investigated radionuclide. Moreover, they can also be used as a baseline for future investigations regarding background radioactivity levels. [ABSTRACT FROM AUTHOR]

In recent years, bioactive compounds have been the focus of much interest in scientific research, due to their low toxicity and extraordinary properties. However, they possess poor solubility, low chemical stability, and unsustainable bioavailability. New drug delivery systems, and among them solid lipid nanoparticles (SLNs), could minimize these drawbacks. In this work, morin (MRN)-loaded SLNs (MRN-SLNs) were prepared using a solvent emulsification/diffusion method, using two different lipids, Compritol® 888 ATO (COM) or Phospholipon® 80H (PHO). SLNs were investigated for their physical–chemical, morphological, and technological (encapsulation parameters and in vitro release) properties. We obtained spherical and non-aggregated nanoparticles with hydrodynamic radii ranging from 60 to 70 nm and negative zeta potentials (about −30 mV and −22 mV for MRN-SLNs-COM and MRN-SLNs-PHO, respectively). The interaction of MRN with the lipids was demonstrated via μ-Raman spectroscopy, X-ray diffraction, and DSC analysis. High encapsulation efficiency was obtained for all formulations (about 99%, w/w), particularly for the SLNs prepared starting from a 10% (w/w) theoretical MRN amount. In vitro release studies showed that about 60% of MRN was released within 24 h and there was a subsequent sustained release within 10 days. Finally, ex vivo permeation studies with excised bovine nasal mucosa demonstrated the ability of SLNs to act as a penetration enhancer for MRN due to the intimate contact and interaction of the carrier with the mucosa. [ABSTRACT FROM AUTHOR]

We present some results, obtained using a multi-scale approach, based on the employment of different and complementary techniques, i.e., Optical Microscopy (OM), Scanning Electron Microscopy-Energy Dispersive X-ray Spectroscopy (SEM-EDS), X-ray diffraction (XRD), Raman and µ-Raman spectroscopy, Fourier transform infrared (FT-IR) spectroscopy equipped with Attenuated Total Reflectance (ATR) analyses, Inductively Coupled Plasma–Mass Spectrometry (ICP-MS), and Thermal Ionization Mass Spectrometry (TIMS), of an integrated activity focused on the characterization of micro-fragments of original and previously restored paintings of the pictorial cycle at the San Panfilo Church in Tornimparte, sampled from specific areas of interest. The study was aimed, on one hand, at the identification of the overlapping restoration materials used during previous conservation interventions (documented and not), and, on the other hand, at understanding the degradation phenomena (current or previous) of the painted surfaces and the architectural structures. The study of stratigraphy allowed us to evaluate the number of layers and the materials (pigments, minerals, and varnishes) present in each layer. As the main result, the identification of blue, black, yellow, and red pigments (both ancient and modern) was achieved. In the case of blue pigments, original (azurite and lazurite) and retouching (Prussian blue and phthalo blue) materials were recognized, together with alteration products (malachite and atacamite). Traces of yellow ochre were found in the yellow areas, and carbon black in the blue and brown areas. In the latter, hematite and red ochre pigments were also recognized. The obtained results are crucial to support the methodological choices during the restoration intervention of the site, and help to ensure the compatibility principles of the materials on which a correct conservative approach is based. [ABSTRACT FROM AUTHOR]

In the present paper, an investigation on the natural and anthropic radioactivity and heavy metals content in a basalt aggregate for concrete from Sicily, Southern Italy, was performed as a case study. In particular, the evaluation of the specific activity of radium-226, thorium-232, potassium-40 and caesium-137 radionuclides was performed by using High-Purity Germanium (HPGe) γ-ray spectrometry, together with the estimation of several indexes developed to evaluate the radiological risk for the population related to radiation exposure, i.e., the alpha index (Iα), the radium equivalent activity (Raeq), the absorbed γ-dose rate (D) and the annual effective dose equivalent outdoor (AEDEout) and indoor (AEDEin). Moreover, measurements of the average heavy metals (arsenic, cadmium, copper, mercury, nickel, lead, antimony, thallium and zinc) concentrations in the analyzed sample were performed by using Inductively Coupled Plasma Mass Spectrometry (ICP-MS). Furthermore, with the aim to investigate any possible chemical pollution, the Enrichment Factor (EF), Geo-accumulation Index (Igeo), Contamination Factor (CF) and Pollution Load Index (PLI) were assessed. Finally, the identification of the source of the aforementioned radioisotopes of natural origin was carried out by X-ray diffraction (XRD), thus identifying the major mineralogical phases present in the investigated basalt aggregate for concrete. [ABSTRACT FROM AUTHOR]

The periodic evaluation of the Faraday cage efficiency for diagnostic Magnetic Resonance is mandatory by Italian legislation and it is recommended by manufacturers. An inadequate shielding could affect clinical images with artifacts, reducing the quality of examinations. The International reference standard for shielding effectiveness measurements is represented by IEEE Standard 299-2006, but it results poorly applicable in Magnetic Resonance sites. In this study, an alternative approach is proposed to simplify the applicability of the IEEE Standard. The experimental set-up for measurements is composed by a signal generator and a transmitting antenna, coupled with the signal generator. The novelty of the proposed methodology consists in the absence of the receiver antenna; for this purpose, the Magnetic Resonance scanner is used. This approach permits to test the entire Faraday cage by means of a single measure. If the Magnetic Resonance device picks up the radiofrequency signal from outside of the cage, this would be visible on the acquired images as a "zipper" artifact. Four Faraday cages, different in building materials, were tested by using the proposed methodology, and the results were compared with those obtained by using the IEEE Standard. The presence of the "zipper" artifact on the images made us inspect the entire Faraday cage and not just the openings, finding that the radiofrequency signal entered from an area without openings, thus denoting a poor construction of the cage. The proposed methodology provides an immediate evaluation of the shielding efficiency, furnishing information on the entire Faraday cage and not only on its apertures. The method is simple, easily reproducible, and constitutes a valid alternative to the IEEE Standard; in particular, during constancy tests. [ABSTRACT FROM AUTHOR]

In this article, the assessment of the radioactivity content in bovine meat from the Calabria region, Southern Italy, was performed. For this purpose, High Purity Germanium (HPGe) gamma spectrometry measurements were carried out in order to investigate any possible radioactive contamination by natural (40K) and anthropogenic (137Cs) radionuclides. Experimental mean values were found to be in the range from (78.9 ± 10.5) Bq kg−1 to (88.2 ± 12.5) Bq kg−1 for 40K and lower than the minimum detectable activity (MDA) in all cases for 137Cs, respectively. Moreover, any possible radiological health risk was also estimated, by calculating the total annual effective dose due to the ingestion of bovine meat by adult members of the population and by comparing it with the total natural radioactivity value (external + internal) for humans. Obtained values are in the range from 10.3 µSv y−1 to 11.5 µSv y−1, several orders of magnitude lower than the value of the total exposure to natural radioactivity for human beings, i.e., 2.4 mSv y−1. It is worth noting that the used approach could be used, in principle, for the evaluation of the radiological risk due to the presence of radionuclides in a large variety of food samples of particular interest, and thus it can constitute a guideline for investigations focused on the monitoring of food quality. [ABSTRACT FROM AUTHOR]

The Museo Egizio's collection contains 200 precious and unique leather artifacts belonging to different historical periods. The materials used during the tanning and curing procedures affect the chemical and elemental composition of the surface of the samples as well as their preservation status, specifically through the hydration level within the bulk. Here we provide an experimental characterization of a series of samples from Museo Egizio that document an extensive denaturation phenomenon (gelatinization), by combining non-destructive techniques including surface probes (X-ray fluorescence, Raman scattering, and scanning electron microscopy enhanced by X-ray energy spectroscopy) and neutron-based bulk techniques (inelastic and deep-inelastic neutron scattering). Results show partial dehydration of the samples in the bulk, affecting the morphology of their surface, the presence of potassium alum, and iron oxides, as well as phosphates and hydroxides related to the tanning and curing procedures. Finally, we briefly discuss the need for a versatile and adaptable software package that is capable of combining quantitative analyses with complementary techniques including morphological, elemental, and chemical composition. [ABSTRACT FROM AUTHOR]

Potentially toxic elements contamination represents a universal problem of major concern, due to several adverse health effects on human beings when permissible concentration levels are overcome. In this sense, the assessment of potentially risky elements content in different environmental matrices plays a key role in the safeguarding of the quality of the environment, and thus of the strictly correlated public health. In this article, measurements of the average potentially toxic elements concentrations in pyroclastic products from Mt. Etna, Eastern Sicily and Southern Italy were performed together with a comparison with the allowable levels set by Italian legislation, with the aim to evaluate the level of toxicity imposed on the ecosystem. For this purpose, Inductively-Coupled Plasma Mass Spectrometry (ICP-MS) measurements were performed to investigate any possible chemical pollution by potentially risky elements, by applying different indices such as Enrichment Factor (EF), Geo-accumulation Index (Igeo), Contamination Factor (CF) and Pollution Load Index (PLI). Finally, the multivariate statistical analysis was performed by processing potentially toxic elements content and pollution indices. It is worth noting that the used approach could be applied, in principle, for the evaluation of the chemical risk due to the presence of potentially toxic elements in a large variety of samples of particular environmental interest, and can constitute a guideline for investigations focused on the monitoring of the environmental quality. [ABSTRACT FROM AUTHOR]

Idebenone (IDE) is a powerful antioxidant that is potentially active towards cerebral diseases, but its low water solubility and fast first pass metabolism reduce its accumulation in the brain, making it ineffective. In this work, we developed cyclodextrin-based chitosan nanospheres (CS NPs) as potential carriers for nose-to-brain targeting of IDE. Sulfobutylether-β-cyclodextrin (SBE-β-CD) was used as a polyanion for chitosan (CS) and as a complexing agent for IDE, permitting its encapsulation into nanospheres (NPs) produced in an aqueous solution. Overloading NPs were obtained by adding the soluble IDE/hydroxypropyl-β-CD (IDE/HP-β-CD) inclusion complex into the CS or SBE-β-CD solutions. We obtained homogeneous CS NPs with a hydrodynamic radius of about 140 nm, positive zeta potential (about +28 mV), and good encapsulation efficiency and drug loading, particularly for overloaded NPs. A biphasic release of IDE, finished within 48 h, was observed from overloaded NPs, whilst non-overloaded CS NPs produced a prolonged release, without a burst effect. In vitro biological studies showed the ability of CS NPs to preserve the antioxidant activity of IDE on U373 culture cells. Furthermore, Fourier transform infrared spectroscopy (FT-IR) demonstrated the ability of CS NPs to interact with the excised bovine nasal mucosa, improving the permeation of the drug and potentially favoring its accumulation in the brain. [ABSTRACT FROM AUTHOR]

The pottery fragments coming from the town of Caltagirone, Italy are characterized by using a nondestructive time-of-flight (TOF) neutron diffraction. The measurements and the Rietveld analysis helped in obtaining a detailed identification and quantitative determination of the mineral phases present in the samples.

The authors describe several applications of FT-IR spectroscopy in the biomedical sciences, including characterization of healthy and neoplastic human skin samples affected by two kinds of cancers, and examination of […]

The diffusional and vibrational dynamics on water confined in a NaA zeolitic matrix is investigated by employing incoherent quasi-elastic neutron scattering (IQENS) and Fourier-transform infrared (FT-IR) absorption measurements. The diffusive process reveals a wide distribution of relaxation times, in analogy with the results of a recent quasi-elastic neutron scattering experiments on water confined in a sol-gel silica glass.

Uncharged star polymers having a porphyrin core and four poly (ethylene glycol) methyl ether (PEGME) arms were synthesized and investigated as a function of the molecular mass of the arms, in different thermodynamic conditions. Molecules with short PEGME arms are insoluble in water, where as in the presence of long PEGME chains, the star polymers are water-soluble for the intermediate length of the arms the building up of large rigid monodisperse aggregates, driven by the diffusion limit aggregation mechanism were observed by means f static and dynamic light scattering.

The natural (40K) and anthropogenic (137Cs) radioactivity concentration in four different typologies of early childhood (up to two years old) foods, i.e., homogenized fruit, homogenized meat, childhood biscuits and baby pasta, produced in Italy and sold in Italian large retailers, was investigated through High Purity Germanium (HPGe) gamma spectrometry. The present study is carried out with the aim to: (i) evaluate the background levels of the investigated radionuclides in the analyzed early childhood foods, (ii) identify whether the twenty analyzed samples were appropriate for infant consumption and (iii) contribute to construct a database on the radioactivity of early childhood foods sold in Italy. [ABSTRACT FROM AUTHOR]