46 results on '"Thomas, Joseph M."'
Search Results
2. The Post-Abolitionist's Narrative: William Greenleaf Eliot's The Story of Archer Alexander
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Thomas, Joseph M.
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- 2000
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3. The Drosophila hep pathway mediates Lrrk2-induced neurodegeneration
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Yang, Dejun, Thomas, Joseph M., Li, Tianxia, Lee, Youngseok, Liu, Zhaohui, and Smith, Wanli W.
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Drosophila -- Health aspects ,Parkinson disease -- Genetic aspects ,RNA interference -- Health aspects ,Gene mutation -- Health aspects ,Biological sciences - Abstract
Although the pathogenesis of Parkinson's disease (PD) remains unclear, mutations in leucine-rich repeat kinase 2 (Lrrk2) are among the major causes of familial PD. Most of these mutations disrupt Lrrk2 kinase and (or) GTPase domain function, resulting in neuronal degeneration. However, the signal pathways underlying Lrrk2-induced neuronal degeneration are not fully understood. There is an expanding body of evidence that suggests a link between Lrrk2 function and MAP kinase (MAPK) cascades. To further investigate this link in vivo, genetic RNAi screens of the MAPK pathways were performed in a Drosophila model to identify genetic modifier(s) that can suppress G2019S-Lrrk2-induced PD-like phenotypes. The results revealed that the knockdown of hemipterous (hep, or JNKK) increased fly survival time, improved locomotor function, and reduced loss of dopaminergic neurons in G2019S-Lrrk2 transgenic flies. Expression of the dominant-negative allele of JNK (JNK-DN), a kinase that is downstream of hep in G2019S-Lrrk2 transgenic flies, elicited a similar effect. Moreover, treatment with the JNK inhibitor SP600125 partially reversed the G2019S-Lrrk2-induced loss of dopaminergic neurons. These results indicate that the hep pathway plays an important role in Lrrk2-linked Parkinsonism in flies. These studies provide new insights into the molecular mechanisms underlying Lrrk2-linked PD pathogenesis and aid in identifying potential therapeutic targets. Key words: Lrrk2, Parkinson's disease, JNK, neuronal degeneration, hep, dopamine neuron, Drosophila model. Meme si la pathogenese de la maladie de Parkinson (MP) demeure floue, des mutations de la proteine Lrrk2 (leucine riche repeat kinase 2) sont parmi les principales causes de la MP hereditaire. La plupart de ces mutations dereglent la fonction des domaines kinase ou GTPase de Lrrk2, resultant en une degeneration neuronale. Toutefois, les voies de signalisation qui sous-tendent la degeneration neuronale induite par Lrrk2 ne sont pas completement comprises. Ilyadeplus en plus de donnees qui suggerent qu'il existe un lien entre la fonction de Lrrk2 et les cascades des MAP kinases (MAPK). Afin d'approfondir ce lien in vivo, des criblages genetiques des voies des MAPK a l'aide d'ARNi ont ete realises chez la drosophile afin d'identifier les modificateurs genetiques qui peuvent supprimer les phenotypes de type parkinsonien induits par G2019S-Lrrk2. Les resultats ont revele que le knockdown de hemipterous (hep ou JNKK) accroissait la duree de vie des mouches, ameliorait la fonction locomotrice et reduisait la perte de neurones dopaminergiques chez les mouches transgeniques G2019S-Lrrk2. L'expression de l'allele dominant negatif de JNK (JNK-DN), une kinase qui agit en aval de hep chez les mouches transgeniques G2019S-Lrrk2, provoquait un effet similaire. De plus, un traitement avec l'inhibiteur de JNK SP600125 renversait partiellement la perte de neurones dopaminergiques induite par G2019S-Lrrk2. Ces resultats indiquent que la voie de hep joue un role important dans le parkinsonisme lie a Lrrk2 chez les mouches. Ces etudes apportent un eclairage nouveau sur les mecanismes moleculaires qui sous-tendent la pathogenese de la MP liee a Lrrk2 et contribuent a identifier des cibles therapeutiques potentielles. [Traduit par la Redaction] Mots-cles : Lrrk2, maladie de Parkinson, JNK, degeneration neuronale, hep, neurone a dopamine, modele de la drosophile., Introduction Parkinson's disease (PD) is one of the most common neurodegenerative disorders and is characterized by the selective loss of dopaminergic neurons and the presence of Lewy bodies. The clinical [...]
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- 2018
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4. "The Property of My Own Book": Emerson's Poems (1847) and the Literary Marketplace
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Thomas, Joseph M. and Emerson, Ralph Waldo
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- 1996
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5. Major Amputations for Extremity Soft-Tissue Sarcoma
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Smith, Henry G., Thomas, Joseph M., Smith, Myles J.F., Hayes, Andrew J., and Strauss, Dirk C.
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- 2017
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6. Regional chemotherapy by isolated limb perfusion prior to surgery compared with surgery and post-operative radiotherapy for primary, locally advanced extremity sarcoma: a comparison of matched cohorts
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Jakob, Jens, Smith, Henry G., Wilkinson, Michelle J., Pencavel, Tim, Miah, Aisha B., Thomas, Joseph M., Tunn, Per-Ulf, Pilz, Lothar R., Strauss, Dirk C., Hohenberger, Peter, and Hayes, Andrew J.
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- 2018
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7. Late Emerson: Selected Poems and the "Emerson Factory"
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Thomas, Joseph M
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- 1998
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8. A Locus on Chromosome 15 Contributes to Acute Ozone-induced Lung Injury in Collaborative Cross Mice.
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Tovar, Adelaide, Smith, Gregory J., Nalesnik, Morgan B., Thomas, Joseph M., McFadden, Kathryn M., Harkema, Jack R., and Kelada, Samir N. P.
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LUNGS ,LOCUS (Genetics) ,LUNG injuries ,CHROMOSOMES ,GENOTYPE-environment interaction ,GENETIC variation - Abstract
Ozone (O
3 )-induced respiratory toxicity varies considerably within the human population and across inbred mouse strains, indicative of gene-environment interactions (GxE). Though previous studies have identified several quantitative trait loci (QTL) and candidate genes underlying responses to O3 exposure, precise mechanisms of susceptibility remain incompletely described. We sought to update our understanding of the genetic architecture of O3 responsiveness using the Collaborative Cross (CC) recombinant inbred mouse panel. We evaluated hallmark O3 -induced inflammation and injury phenotypes in 56 CC strains after exposure to filtered air or 2 ppm O3 , and performed focused genetic analysis of variation in lung injury, as reflected by protein in lung lavage fluid. Strain-dependent responses to O3 were clear, and QTL mapping revealed two novel loci on Chr (Chromosomes) 10 (peak, 26.2 Mb; 80% confidence interval [CI], 24.6–43.6 Mb) and 15 (peak, 47.1 Mb; 80% CI, 40.2–54.9 Mb), the latter surpassing the 95% significance threshold. At the Chr 15 locus, C57BL/6J and CAST/EiJ founder haplotypes were associated with higher lung injury responses compared with all other CC founder haplotypes. With further statistical analysis and a weight of evidence approach, we delimited the Chr 15 QTL to an ~2 Mb region containing 21 genes (10 protein coding) and nominated three candidate genes, namely Oxr1, Rspo2, and Angpt1. Gene and protein expression data further supported Oxr1 and Angpt1 as priority candidate genes. In summary, we have shown that O3 -induced lung injury is modulated by genetic variation, identified two high priority candidate genes, and demonstrated the value of the CC for detecting GxE. [ABSTRACT FROM AUTHOR]- Published
- 2022
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9. Figures of Habit in William James
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Thomas, Joseph M.
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- 1993
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10. Novel LRRK2 GTP-binding inhibitors reduced degeneration in Parkinsonʼs disease cell and mouse models
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Li, Tianxia, Yang, Dejun, Zhong, Shijun, Thomas, Joseph M., Xue, Fengtian, Liu, Jingnan, Kong, Lingbo, Voulalas, Pamela, Hassan, Hazem E., Park, Jae-Sung, MacKerell, Alexander D., Jr, and Smith, Wanli W.
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- 2014
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11. Swift and the Stamp Act of 1712
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Thomas, Joseph M.
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- 1916
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12. Oral Composition in Its Relation to Written
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Thomas, Joseph M.
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- 1915
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13. The Inhibitory Instincts
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Thomas, Joseph M.
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- 1920
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14. Do Thought-Courses Produce Thinking?
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Thomas, Joseph M.
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- 1916
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15. Preliminary Report of the Special Committee on Freshman English
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Scott, Frank W., Thomas, Joseph M., and Manchester, Frederick A.
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- 1918
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16. Integrative analysis reveals mouse strain-dependent responses to acute ozone exposure associated with airway macrophage transcriptional activity.
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Tovar, Adelaide, Crouse, Wesley L., Smith, Gregory J., Thomas, Joseph M., Keith, Benjamin P., McFadden, Kathryn M., Moran, Timothy P., Furey, Terrence S., and Kelada, Samir N. P.
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GENOTYPE-environment interaction ,OZONE ,MICE ,MACROPHAGES ,GENE expression ,CHROMATIN - Abstract
Acute ozone (O
3 ) exposure is associated with multiple adverse cardiorespiratory outcomes, the severity of which varies across individuals in human populations and inbred mouse strains. However, molecular determinants of response, including susceptibility biomarkers that distinguish who will develop severe injury and inflammation, are not well characterized. We and others have demonstrated that airway macrophages (AMs) are an important resident immune cell type that are functionally and transcriptionally responsive to O3 inhalation. Here, we sought to explore influences of strain, exposure, and strain-by-O3 exposure interactions on AM gene expression and identify transcriptional correlates of O3 -induced inflammation and injury across six mouse strains, including five Collaborative Cross (CC) strains. We exposed adult mice of both sexes to filtered air (FA) or 2 ppm O3 for 3 h and measured inflammatory and injury parameters 21 h later. Mice exposed to O3 developed airway neutrophilia and lung injury with strain-dependent severity. In AMs, we identified a common core O3 transcriptional response signature across all strains, as well as a set of genes exhibiting strain-by-O3 exposure interactions. In particular, a prominent gene expression contrast emerged between a low- (CC017/Unc) and high-responding (CC003/Unc) strain, as reflected by cellular inflammation and injury. Further inspection indicated that differences in their baseline gene expression and chromatin accessibility profiles likely contribute to their divergent post-O3 exposure transcriptional responses. Together, these results suggest that aspects of O3 - induced respiratory responses are mediated through altered AM transcriptional signatures and further confirm the importance of gene-environment interactions in mediating differential responsiveness to environmental agents. [ABSTRACT FROM AUTHOR]- Published
- 2022
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17. SURGERY FOR GASTROINTESTINAL STROMAL TUMOUR IN THE POST-IMATINIB ERA
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NEUHAUS, SUSAN J., CLARK, MATTHEW A., HAYES, ANDREW J., THOMAS, JOSEPH M., and JUDSON, IAN
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- 2005
18. 'Peculiar soil': mining the early American imagination
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Thomas, Joseph M.
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American literature -- Colonial period, ca. 1600-1775 ,Mining industry -- Portrayals - Published
- 1992
19. Kinetics for the reactions of H30+ (H20)n with CH3SOCH3
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Thomas, Joseph M. and Viggiano, A.A.
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Dimethyl sulfoxide -- Research ,Ions -- Research ,Chemical reaction, Rate of -- Research ,Chemicals, plastics and rubber industries - Abstract
The rate constants and product branching functions for the proton hydrate clusters H3O+(H20)n (n = 0-4), reacting with dimethyl sulfoxide were measured. The reactions were studied over the temperature range 198-298 K. The instability of large clusters at higher temperatures only permitted their study at low temperatures. All reactions were fast, approaching the collisional limit. The small clusters were slightly slower than the collision limit especially at 298 K.
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- 1999
20. GTP‐binding inhibitors increase LRRK2‐linked ubiquitination and Lewy body‐like inclusions.
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Thomas, Joseph M., Wang, Xiaobo, Guo, Gongbo, Li, Tianxia, Dai, Bingling, Nucifora, Leslie G., Nucifora, Frederick C., Liu, Zhaohui, Xue, Fengtian, Liu, Chunfeng, Ross, Christopher A., and Smith, Wanli W.
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DARDARIN , *UBIQUITINATION , *DOPAMINERGIC neurons , *PARKINSON'S disease - Abstract
Parkinson's disease (PD) is one of the most common movement disorders with loss of dopaminergic neurons and the presence of Lewy bodies in certain brain areas. However, it is not clear how Lewy body (inclusion with protein aggregation) formation occurs. Mutations in leucine‐rich repeat kinase 2 (LRRK2) can cause a genetic form of PD and contribute to sporadic PD with the typical Lewy body pathology. Here, we used our recently identified LRRK2 GTP‐binding inhibitors as pharmacological probes to study the LRRK2‐linked ubiquitination and protein aggregation. Pharmacological inhibition of GTP‐binding by GTP‐binding inhibitors (68 and Fx2149) increased LRRK2‐linked ubiquitination predominantly via K27 linkage. Compound 68‐ or Fx2149 increased G2019S‐LRRK2‐linked ubiquitinated aggregates, which occurred through the atypical linkage types K27 and K63. Coexpression of K27R and K63R, which prevented ubiquitination via K27 and K63 linkages, reversed the effects of 68 and Fx2149. Moreover, 68 and Fx2149 also promoted G2019S‐LRRK2‐linked aggresome (Lewy body‐like inclusion) formation via K27 and K63 linkages. These findings demonstrate that LRRK2 GTP‐binding activity is critical in LRRK2‐linked ubiquitination and aggregation formation. These studies provide novel insight into the LRRK2‐linked Lewy body‐like inclusion formation underlying PD pathogenesis. [ABSTRACT FROM AUTHOR]
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- 2020
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21. Transcriptional Profiling of the Murine Airway Response to Acute Ozone Exposure.
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Tovar, Adelaide, Smith, Gregory J, Thomas, Joseph M, Crouse, Wesley L, Harkema, Jack R, and Kelada, Samir N P
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OZONE ,GENE expression ,EXTRACELLULAR matrix ,CELL proliferation ,PERMEABILITY ,TIGHT junctions - Abstract
Ambient ozone (O
3 ) exposure has serious consequences on respiratory health, including airway inflammation and injury. Decades of research have yielded thorough descriptions of these outcomes; however, less is known about the molecular processes that drive them. The aim of this study was to further describe the cellular and molecular responses to O3 exposure in murine airways, with a particular focus on transcriptional responses in 2 critical pulmonary tissue compartments: conducting airways (CA) and airway macrophages (AM). After exposing adult, female C57BL/6J mice to filtered air, 1 or 2 ppm O3 , we assessed hallmark responses including airway inflammation (cell counts and cytokine secretion) and injury (epithelial permeability), followed by gene expression profiling of CA and AM by RNA-seq. As expected, we observed concentration-dependent increases in airway inflammation and injury. Conducting airways and AM both exhibited changes in gene expression to both 1 and 2 ppm O3 that were largely compartment-specific. In CA, genes associated with epithelial barrier function, detoxification processes, and cellular proliferation were altered, while O3 affected genes involved in innate immune signaling, cytokine production, and extracellular matrix remodeling in AM. Further, CA and AM also exhibited notable differences in concentration–response expression patterns for large numbers of genes. Overall, our study has described transcriptional responses to acute O3 exposure, revealing both shared and unique gene expression patterns across multiple concentrations of O3 and in 2 important O3 -responsive tissues. These profiles provide broad mechanistic insight into pulmonary O3 toxicity, and reveal a variety of targets for focused follow-up studies. [ABSTRACT FROM AUTHOR]- Published
- 2020
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22. Rotational analysis of the nonthermal NO(A 2Σ+, v’=0) distribution observed in the N2(A 3Σ+u, v’=0)+NO(X 2Π, v‘=0) energy transfer reaction.
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Thomas, Joseph M. and Katayama, Daniel H.
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ENERGY transfer , *NITRIC oxide , *PHOTOELECTRICITY - Abstract
A rapidly pumped discharge-flow reactor was constructed to characterize the N2(A 3Σ+u,v’)+NO(X 2Π, v‘=0) energy transfer (ET) reaction. Emission spectra of the NO γ bands from the product NO A 2Σ state formed in the title reaction were collected with a 2.2 m vacuum-ultraviolet spectrograph monochromator utilizing both photographic and photoelectric techniques. The rotational structure of the γ bands resulting from the title reaction is resolved with a spectral resolution of Δλ∼0.1 Å. The product NO(A,v’) emission spectra were measured as a function of total pressure (∼0.8≤pTOTAL≤∼3.0 Torr) and initial N2(A,v’) population distribution at 298 K. The product NO(A,v’,N’) rotational distributions yield temperatures much higher than room temperature (>1600 K) and are discussed in detail. Vibrational level specific bimolecular rate constants kv’ ’s for the N2(A,4≤v’≤6)+NO reaction were measured using N2(B 3Πg–A 3Σ+u) laser-excited fluorescence detection with a fixed reaction time. The kv’ ’s are (9.5±1.0), (11.3±1.2), and (11.2±1.1)×10-11 cm3 molecule-1 s-1 for v’=4, 5, and 6, respectively. A comparison with previous measurements is presented. [ABSTRACT FROM AUTHOR]
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- 1991
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23. Rate constants for electronic quenching of N2(A 3Σ+u, v=0–6) by O2, NO, CO, N2O, and C2H4.
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Thomas, Joseph M., Kaufman, Frederick, and Golde, Michael F.
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FLUORESCENCE , *VIBRATION (Mechanics) , *QUANTUM theory , *ENERGY transfer - Abstract
Rate constants for the title reactions have been measured using the discharge-flow technique, with N2(B 3Πg –A 3Σ+u) laser-excited fluorescence detection. C2H4 is an efficient quencher and exhibits little dependence of rate constant on vibrational quantum number v in N2(A). The rate constants for NO, O2, and N2O increase with v at low v, but are nearly independent of v for v≥3. CO shows a very strong dependence, with a peak in the rate constant at v=2 and a trough at v=5. These are the first data for the reactions of N2(A, v=2–6) with N2O. The other data are compared with previous measurements and discussed in terms of models of electronic-to-electronic energy transfer. [ABSTRACT FROM AUTHOR]
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- 1987
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24. The Drosophilahep pathway mediates Lrrk2-induced neurodegeneration.
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Yang, Dejun, Thomas, Joseph M., Li, Tianxia, Lee, Youngseok, Liu, Zhaohui, and Smith, Wanli W.
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PARKINSON'S disease ,NEURODEGENERATION ,BRAIN diseases ,DARDARIN ,DOPAMINERGIC neurons ,DROSOPHILA - Abstract
Copyright of Biochemistry & Cell Biology is the property of Canadian Science Publishing and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)
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- 2018
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25. Differential Regulation of Zfp30 Expression in Murine Airway Epithelia Through Altered Binding of ZFP148 to rs51434084.
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Laudermilk, Lucas T., Thomas, Joseph M., and Kelada, Samir N.
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ZINC-finger proteins , *GENE expression , *HAPLOTYPES - Abstract
Neutrophil chemotaxis to the airways is a key aspect of host response to microbes and a feature of multiple pulmonary diseases including asthma. Tight regulation of this recruitment is critical to prevent unwanted host tissue damage and inflammation. Using a mouse (Mus musculus) model of asthma applied to the Collaborative Cross population, we previously identified a lung gene expression quantitative trait locus (eQTL) for Zinc finger protein 30 (Zfp30) that was also a QTL for neutrophil recruitment and the hallmark neutrophil chemokine CXCL1. The Zfp30 eQTL is defined by three functionally distinct haplotypes. In this study, we searched for causal genetic variants that underlie the Zfp30 eQTL to gain a better understanding of this candidate repressor's regulation. First, we identified a putative regulatory region spanning 500 bp upstream of Zfp30, which contains 10 SNPs that form five haplotypes. In reporter gene assays in vitro, these haplotypes recapitulated the three previously identified in vivo expression patterns. Second, using site-directed mutagenesis followed by reporter gene assays, we identified a single variant, rs51434084, which explained the majority of variation in expression between two out of three haplotype groups. Finally, using a combination of in silico predictions and electrophoretic mobility shift assays, we identified ZFP148 as a transcription factor that differentially binds to the Zfp30 promoter region harboring rs51434084. In conclusion, we provide evidence in support of rs51434084 being a causal variant for the Zfp30 eQTL, and have identified a mechanism by which this variant alters Zfp30 expression, namely differential binding of ZFP148. [ABSTRACT FROM AUTHOR]
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- 2018
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26. Major Amputations for Extremity Soft-Tissue Sarcoma.
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Smith, Henry G., Thomas, Joseph M., Smith, Myles J.F., Hayes, Andrew J., and Strauss, Dirk C.
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Introduction: With modern techniques facilitating limb conservation, amputation for extremity soft-tissue sarcoma (ESTS) is now rare. We sought to determine the indications and outcomes following major amputation for ESTS and whether amputation is prognostic of oncological outcomes in primary disease. Patients and Methods: Patients undergoing major amputations for ESTS from 2004 to 2014 were identified from electronic patient records. Results: The amputation rate in primary localized disease was 4.1%. Overall, 69 patients were identified, including 23 (33.3%) amputations for primary localized disease, 36 (52.2%) amputations for recurrent disease, and 10 (14.5%) amputations for metastatic disease. The local recurrence rate for localized disease at 3 years was 10.4%. Three-year overall survival (OS) was 50.3% following curative amputation, with a median survival of 41 months, and median OS following palliative amputation was 6 months. In the context of primary, localized disease, patients undergoing amputation had a greater proportion of high-grade tumors (69.6% vs. 41.1%; p = 0.009) of greater size (median 16.0 vs. 9.0 cm; p = 0.003) when compared with patients undergoing limb-conserving surgery. The rates of systemic relapse and disease-specific survival were poorer following amputation compared with limb-conserving surgery, however mode of surgery (amputation vs. limb conservation) was only prognostic for OS. Conclusions: Amputation maintains an important role in ESTS and achieves durable local control in those unsuitable for limb-conserving surgery. Survival following amputation in the presence of metastatic disease is poor and should be reserved for patients with significant symptoms. [ABSTRACT FROM AUTHOR]
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- 2018
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27. 68 and FX2149 Attenuate Mutant LRRK2-R1441C-Induced Neural Transport Impairment.
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Thomas, Joseph M., Tianxia Li, Wei Yang, Fengtian Xue, Fishman, Paul S., Smith, Wanli W., Reddy, P. Hemachandra, and Thomas, Bobby
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PARKINSON'S disease ,DARDARIN ,NEURAL transmission ,MITOCHONDRIA ,GENETIC mutation - Abstract
Leucine-rich repeat kinase 2 is a large protein with implications in genetic and sporadic causes of Parkinson's disease. The physiological functions of LRRK2 are largely unknown. In this report, we investigated whether LRRK2 alters neural transport using live-cell imaging techniques and human neuroblastoma SH-SY5Y cells. Our results demonstrated that expression of the PD-linked mutant, LRRK2-R1441C, induced mitochondrial, and lysosomal transport defects in neurites of SH-SY5Y cells. Most importantly, recently identified GTP-binding inhibitors, 68 and FX2149, can reduce LRRK2 GTP-binding activity and attenuates R1441C-induced mitochondrial and lysosomal transport impairments. These results provide direct evidence and an early mechanism for neurite injury underlying LRRK2-induced neurodegeneration. This is the first report to show that LRRK2 GTP-binding activity plays a critical role during neurite transport, suggesting inhibition of LRRK2 GTP-binding could be a potential novel strategy for PD intervention. [ABSTRACT FROM AUTHOR]
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- 2017
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28. Selective marginal resections in the management of aggressive angiomyxomas.
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Smith, Henry G., Thway, Khin, Messiou, Christina, Barton, Desmond P., Thomas, Joseph M., Hayes, Andrew J., Strauss, Dirk C., and Smith, Myles J.F.
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- 2016
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29. Synphilin-1 attenuates mutant LRRK2-induced neurodegeneration in Parkinson's disease models.
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Jingnan Liu, Tianxia Li, Thomas, Joseph M., Zhong Pei, Haibing Jiang, Engelender, Simone, Ross, Christopher A., and Smith, Wanli W.
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- 2016
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30. A Novel GTP-Binding Inhibitor, FX2149, Attenuates LRRK2 Toxicity in Parkinson’s Disease Models.
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Li, Tianxia, He, Xinhua, Thomas, Joseph M., Yang, Dejun, Zhong, Shijun, Xue, Fengtian, and Smith, Wanli W.
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PARKINSON'S disease ,NEURODEGENERATION ,G proteins ,TOXICITY testing ,LEUCINE ,GENETIC mutation - Abstract
Leucine-rich repeat kinase-2 (LRRK2), a cytoplasmic protein containing both GTP binding and kinase activities, has emerged as a highly promising drug target for Parkinson’s disease (PD). The majority of PD-linked mutations in LRRK2 dysregulate its GTP binding and kinase activities, which may contribute to neurodegeneration. While most known LRRK2 inhibitors are developed to target the kinase domain, we have recently identified the first LRRK2 GTP binding inhibitor, 68, which not only inhibits LRRK2 GTP binding and kinase activities with high potency in vitro, but also reduces neurodegeneration. However, the in vivo effects of 68 are low due to its limited brain penetration. To address this problem, we reported herein the design and synthesis of a novel analog of 68, FX2149, aimed at increasing the in vivo efficacy. Pharmacological characterization of FX2149 exhibited inhibition of LRRK2 GTP binding activity by ~90% at a concentration of 10 nM using in vitro assays. Furthermore, FX2149 protected against mutant LRRK2-induced neurodegeneration in SH-SY5Y cells at 50-200 nM concentrations. Importantly, FX2149 at 10 mg/kg (i.p.) showed significant brain inhibition efficacy equivalent to that of 68 at 20 mg/kg (i.p.), determined by mouse brain LRRK2 GTP binding and phosphorylation assays. Furthermore, FX2149 at 10 mg/kg (i.p.) attenuated lipopolysaccharide (LPS)-induced microglia activation and LRRK2 upregulation in a mouse neuroinflammation model comparable to 68 at 20 mg/kg (i.p.). Our results highlight a novel GTP binding inhibitor with better brain efficacy, which represents a new lead compound for further understanding PD pathogenesis and therapeutic studies. [ABSTRACT FROM AUTHOR]
- Published
- 2015
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31. AN ANALYSIS OF A RAPID, SIMPLE, AND INEXPENSIVE TECHNIQUE USED TO OBTAIN PLATELET-RICH PLASMA FOR USE IN CLINICAL PRACTICE.
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Rutkowski, James L., Thomas, Joseph M., Bering, C. Larry, Speicher, Julie L., Radio, Nicholas M., Smith, Douglas M., and Johnson, David A.
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BLOOD platelets ,BLOOD plasma ,GROWTH factors ,WOUND healing ,NEOVASCULARIZATION - Abstract
The use of platelet-rich plasma (PRP) has become more generally accepted, and implant dentists are using PRP more frequently to promote the healing of oral surgical and/or periodontal wounds. Critical elements of PRP are thought to be growth factors contained within the concentrated platelets. These growth factors are known to promote soft-tissue healing, angiogenesis and osteogenesis. We present a rapid, simple, and inexpensive methodology for preparing PRP using the Cliniseal centrifuge method. This study demonstrates that platelets are concentrated approximately 6-fold without altering platelet morphology. Further we demonstrate that key growth factors, platelet-derived growth factor BB (PDGFBB), transforming growth factor B (TGF-B1), vasculature endothelial growth factor (VEGF), and epidermal growth factor (EGF) are present in comparable or higher concentrations than those reported with the use of other techniques. Prolonged bench set time (.3 hours) after centrifugation resulted in decreased concentration of TGF-B1 but not decreased concentration of PDGF-BB, VEGF, or EGF. This study confirms the molecular aspects of PRP obtained using this inexpensive and efficient methodology. [ABSTRACT FROM AUTHOR]
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- 2008
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32. Late Emerson: Selected Poems and the `Emerson Factory'.
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Thomas, Joseph M.
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POETRY (Literary form) , *CRITICISM - Abstract
Argues that poetry was a more congenial medium for Ralph Waldo Emerson at the point in his life when he prepared his 1876 book `Selected Poems.' Representations of Emerson's identity as a writer at the end of his career; 20th-century readers' underappreciation of Emerson's poetry; Publication of `May-Day and Other Pieces' in 1867; Ellen Emerson's description of the toll exacted by age and overwork.
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- 1998
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33. `The Property of My Own Book': Emerson's Poems (1847) and the literary marketplace.
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Thomas, Joseph M.
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CRITICISM - Abstract
Presents a critique of writer Ralph Waldo Emerson. Focus on Emerson's book `Poems'; De-transcendentalization of Emerson; Michel Gilmore's treatment of Emerson's profession of authorship in the book `American Romanticism and the Marketplace'; Emerson's approach to the literary marketplace.
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- 1996
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34. A LRRK2 GTP Binding Inhibitor, 68, Reduces LPS-Induced Signaling Events and TNF-α Release in Human Lymphoblasts.
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Li, Tianxia, Ning, Bo, Kong, Lingbo, Dai, Bingling, He, Xiaofei, Thomas, Joseph M., Sawa, Akira, Ross, Christopher A., Smith, Wanli W., and Morari, Michele
- Subjects
GUANOSINE triphosphate ,CROHN'S disease ,PARKINSON'S disease ,RECESSIVE genes ,B cells ,TOLL-like receptors ,PHOSPHORYLATION - Abstract
Mutations in the leucine-rich repeat kinase-2 (LRRK2) gene cause autosomal-dominant Parkinson's disease (PD) and contribute to sporadic PD. Common genetic variation in LRRK2 modifies susceptibility to immunological disorders including Crohn's disease and leprosy. Previous studies have reported that LRRK2 is expressed in B lymphocytes and macrophages, suggesting a role for LRRK2 in immunological functions. In this study, we characterized the LRRK2 protein expression and phosphorylation using human lymphoblasts. Lipopolysaccharide (LPS), a proinflammatory agent, induced the increase of LRRK2 expression and kinase activities in human lymphoblasts in a time-dependent manner. Moreover, LPS activated the Toll-like receptor (TLR) signaling pathway, increased TRAF6/LRRK2 interaction, and elevated the phosphorylation levels of MAPK (JNK1/2, p38, and ERK1/2) and IkBα. Treatment with LRRK2 inhibitor 68 reduced LPS-induced TRAF6/LRRK2 interaction and MAPK and IkBα phosphorylation, thereby reducing TNF-α secretion. These results indicate that LRRK2 is actively involved in proinflammatory responses in human lymphoblasts, and inhibition of GTP binding by 68 results in an anti-inflammation effect against proinflammatory stimuli. These findings not only provide novel insights into the mechanisms of LRRK2-linked immune and inflammatory responses in B-cell-like lymphoblasts, but also suggest that 68 may also have potential therapeutic value for LRRK2-linked immunological disorders. [ABSTRACT FROM AUTHOR]
- Published
- 2021
- Full Text
- View/download PDF
35. A Case Study of the Effectiveness of a Traffic-Responsive Computerized Traffic Control System
- Author
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Parsonson, Peter S. and Thomas, Joseph M., Jr.
- Published
- 1976
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36. Vibrational relaxation of N 2(A 3Σ u+,υ = 1, 2,3) by CH 4 and CF 4
- Author
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Thomas, Joseph M., Jeffries, Jay B., and Kaufman, Frederick
- Published
- 1983
- Full Text
- View/download PDF
37. Branching fractions of the product [formula omitted] and [formula omitted] emissions observed as a function of ν′ in the energy transfer reaction [formula omitted]
- Author
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Thomas, Joseph M. and Katayama, Daniel H.
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- 1995
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- View/download PDF
38. Reactions of H 3O +(H 2O) n and H +(H 2O) n(CH 3COCH 3) m with CH 3SCH 3
- Author
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Arnold, Susan T, Thomas, Joseph M, and Viggiano, A.A
- Published
- 1998
- Full Text
- View/download PDF
39. Surfactant Protein A Modulates Induction of Regulatory T Cells via TGF-&bgr;.
- Author
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Mukherjee, Sambuddho, Giamberardino, Charles, Thomas, Joseph M., Gowdy, Kymberly, Pastva, Amy M., and Wright, Jo Rae
- Subjects
- *
SURFACE active agents , *T cells , *TRANSFORMING growth factors , *GENE expression , *INTERLEUKIN-2 , *LABORATORY mice - Abstract
TCR signaling plays a critical role in regulatory T cell (Treg) development. However, the mechanism for tissue-specific induction of Tregs in the periphery remains unclear. We observed that surfactant protein A (SP-A)-deficient mice have impaired expression of Foxp3 and fewer CD25+Foxp3+ Tregs after ex vivo stimulation and after stimulation with LPS in vivo. The addition of exogenous SP-A completely reversed this phenotype. Although SP-A is known to inhibit T cell proliferation under certain activation conditions, both IL-2 levels as well as active TGF-β levels increase on extended culture with exogenous SP-A, providing a key mechanism for the maintenance and induction of Tregs. In addition, kinetic suppression assays demonstrate that SP-A enhances the frequency of functional Foxp3+ Tregs in responder T cell populations in a TGF-β-dependent manner. In mice treated with LPS in vivo, Tregs increased ∼160% in wild-type mice compared with only a 50% increase in LPS-treated SP-A-/- mice 8 d after exposure. Taken together, these findings support the hypothesis that SP-A affects T cell immune function by the induction of Tregs during activation. [ABSTRACT FROM AUTHOR]
- Published
- 2012
- Full Text
- View/download PDF
40. Identification of trans Protein QTL for Secreted Airway Mucins in Mice and a Causal Role for Bpifb1.
- Author
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Donoghue, Lauren J., Livraghi-Butrico, Alessandra, McFadden, Kathryn M., Thomas, Joseph M., Gang Chen, Grubb, Barbara R., O'Neal, Wanda K., Boucher, Richard C., and Kelada, Samir N. P.
- Subjects
- *
MUCINS , *MICE , *AIRWAY (Anatomy) , *GLYCOPROTEINS , *GENETICS , *DISEASES - Abstract
Mucus hyper-secretion is a hallmark feature of asthma and other muco-obstructive airway diseases. The mucin proteins MUC5AC and MUC5B are the major glycoprotein components of mucus and have critical roles in airway defense. Despite the biomedical importance of these two proteins, the loci that regulate them in the context of natural genetic variation have not been studied. To identify genes that underlie variation in airway mucin levels, we performed genetic analyses in founder strains and incipient lines of the Collaborative Cross (CC) in a house dust mite mouse model of asthma. CC founder strains exhibited significant differences in MUC5AC and MUC5B, providing evidence of heritability. Analysis of gene and protein expression of Muc5ac and Muc5b in incipient CC lines (n = 154) suggested that post-transcriptional events were important regulators of mucin protein content in the airways. Quantitative trait locus (QTL) mapping identified distinct, trans protein QTL for MUC5AC (chromosome 13) and MUC5B (chromosome 2). These two QTL explained 18 and 20% of phenotypic variance, respectively. Examination of the MUC5B QTL allele effects and subsequent phylogenetic analysis allowed us to narrow the MUC5B QTL and identify Bpifb1 as a candidate gene. Bpifb1 mRNA and protein expression were upregulated in parallel to MUC5B after allergen challenge, and Bpifb1 knockout mice exhibited higher MUC5B expression. Thus, BPIFB1 is a novel regulator of MUC5B. [ABSTRACT FROM AUTHOR]
- Published
- 2017
- Full Text
- View/download PDF
41. Novel Role for Surfactant Protein A in Gastrointestinal Graft-versus-Host Disease.
- Author
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Gowdy, Kymberly M., Cardona, Diana M., Nugent, Julia L., Giamberardino, Charles, Thomas, Joseph M., Mukherjee, Sambudho, Martinu, Tereza, Foster, W. Michael, Plevy, Scott E., Pastva, Amy M., Wright, Jo Rae, and Palmer, Scott M.
- Subjects
- *
SURFACE active agents , *GRAFT versus host disease , *GASTROINTESTINAL diseases , *LABORATORY mice , *T cells , *IMMUNE response , *BONE marrow transplantation - Abstract
Graft-versus-host disease (GVHD) is a severe and frequent complication of allogeneic bone marrow transplantation (BMT) that involves the gastrointestinal (GI) tract and lungs. The pathobiology of GVHD is complex and involves immune cell recognition of host Ags as foreign. We hypothesize a central role for the coilectin surfactant protein A (SP-A) in regulating the development of GVHD after allogeneic BMT. C57BL/6 (H2b; WT) and SP-A-deficient mice on a C57BL/6 background (H2b; SP-A-/-) mice underwent allogeneic or syngeneic BMT with cells from either C3HeB/FeJ (H2k; SP-A-deficient recipient mice that have undergone an allogeneic BMT [SP-A-/-alloBMT] or SP-A-sufficient recipient mice that have undergone an allogeneic BMT) or C57BL/ 6 (H2b; SP-A-deficient recipient mice that have undergone a syngeneic BMT or SP-A-sufficient recipient mice that have undergone a syngeneic BMT) mice. Five weeks post-BMT, mice were necropsied, and lung and GI tissue were analyzed. SP-A-/- alloBMT or SP-A-sufficient recipient mice that have undergone an allogeneic BMT had no significant differences in lung pathology; however, SP-A-/-alloBMT mice developed marked features of GI GVHD, including decreased body weight, increased tissue inflammation, and lymphocytic infiltration. SP-A-/-alloBMT mice also had increased colon expression of IL-1β, IL-6, TNF-a, and IFN-7 and as well as increased Thl7 cells and diminished regulatory T cells. Our results demonstrate the first evidence, to our knowledge, of a critical role for SP-A in modulating GI GVHD. In these studies, we demonstrate that mice deficient in SP-A that have undergone an allogeneic BMT have a greater incidence of GI GVHD that is associated with increased Thl7 cells and decreased regulatory T cells. The results of these studies demonstrate that SP-A protects against the development of GI GVHD and establishes a role for SP-A in regulating the immune response in the GI tract. [ABSTRACT FROM AUTHOR]
- Published
- 2012
- Full Text
- View/download PDF
42. Synphilin-1 attenuates mutant LRRK2-induced neurodegeneration in Parkinson's disease models.
- Author
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Liu J, Li T, Thomas JM, Pei Z, Jiang H, Engelender S, Ross CA, and Smith WW
- Subjects
- Animals, Animals, Genetically Modified, Carrier Proteins genetics, Cell Line, Tumor, Disease Models, Animal, Dopamine metabolism, Drosophila, Gene Knockdown Techniques, HEK293 Cells, Humans, Leucine-Rich Repeat Serine-Threonine Protein Kinase-2 genetics, Mice, Mutation, Nerve Degeneration genetics, Nerve Tissue Proteins genetics, Neurons enzymology, Neurons metabolism, Neurons pathology, Parkinson Disease enzymology, Parkinson Disease genetics, Parkinson Disease pathology, Phosphorylation, Protein Interaction Domains and Motifs, alpha-Synuclein genetics, alpha-Synuclein metabolism, Carrier Proteins metabolism, Leucine-Rich Repeat Serine-Threonine Protein Kinase-2 metabolism, Nerve Tissue Proteins metabolism, Parkinson Disease metabolism
- Abstract
Mutations in leucine-rich repeat kinase 2 (LRRK2) cause autosomal-dominant Parkinsonism with pleomorphic pathology including deposits of aggregated protein and neuronal degeneration. The pathogenesis of LRRK2-linked Parkinson's disease (PD) is not fully understood. Here, using co-immunoprecipitation, we found that LRRK2 interacted with synphilin-1 (SP1), a cytoplasmic protein that interacts with α-synuclein and has implications in PD pathogenesis. LRRK2 interacted with the N-terminus of SP1 whereas SP1 predominantly interacted with the C-terminus of LRRK2, including kinase domain. Co-expression of SP1 with LRRK2 increased LRRK2-induced cytoplasmic aggregation in cultured cells. Moreover, SP1 also attenuated mutant LRRK2-induced toxicity and reduced LRRK2 kinase activity in cultured cells. Knockdown of SP1 by siRNA enhanced LRRK2 neuronal toxicity. In vivo Drosophila studies, co-expression of SP1 and mutant G2019S-LRRK2 in double transgenic Drosophila increased survival and improved locomotor activity. Expression of SP1 protects against G2019S-LRRK2-induced dopamine neuron loss and reduced LRRK2 phosphorylation in double transgenic fly brains. Our findings demonstrate that SP1 attenuates mutant LRRK2-induced PD-like phenotypes and plays a neural protective role., (© The Author 2016. Published by Oxford University Press. All rights reserved. For Permissions, please email: journals.permissions@oup.com.)
- Published
- 2016
- Full Text
- View/download PDF
43. Interleukin-13 induces collagen type-1 expression through matrix metalloproteinase-2 and transforming growth factor-β1 in airway fibroblasts in asthma.
- Author
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Firszt R, Francisco D, Church TD, Thomas JM, Ingram JL, and Kraft M
- Subjects
- Actins metabolism, Adult, Biopsy, Bronchi metabolism, Female, Fibroblasts metabolism, Humans, Male, Respiratory Function Tests, Asthma metabolism, Collagen Type I metabolism, Extracellular Matrix Proteins metabolism, Gene Expression Regulation, Interleukin-13 metabolism, Matrix Metalloproteinase 2 metabolism, Transforming Growth Factor beta metabolism
- Abstract
Airway remodelling is a feature of asthma that contributes to loss of lung function. One of the central components of airway remodelling is subepithelial fibrosis. Interleukin (IL)-13 is a key T-helper 2 cytokine and is believed to be the central mediator of allergic asthma including remodelling, but the mechanism driving the latter has not been elucidated in human asthma. We hypothesised that IL-13 stimulates collagen type-1 production by the airway fibroblast in a matrix metalloproteinase (MMP)- and transforming growth factor (TGF)-β1-dependent manner in human asthma as compared to healthy controls. Fibroblasts were cultured from endobronchial biopsies in 14 subjects with mild asthma and 13 normal controls that underwent bronchoscopy. Airway fibroblasts were treated with various mediators including IL-13 and specific MMP-inhibitors. IL-13 significantly stimulated collagen type-1 production in asthma compared to normal controls. Inhibitors of MMP-2 significantly attenuated collagen production in asthma but had no effect in normal controls. IL-13 significantly increased total and active forms of TGF-β1, and this activation was blocked using an MMP-2 inhibitor. IL-13 activated endogenous MMP-2 in asthma patients as compared to normal controls. In an ex vivo model, IL-13 potentiates airway remodelling through a mechanism involving TGF-β1 and MMP-2. These effects provide insights into the mechanism involved in IL-13-directed airway remodelling in asthma.
- Published
- 2014
- Full Text
- View/download PDF
44. Surfactant protein-A inhibits mycoplasma-induced dendritic cell maturation through regulation of HMGB-1 cytokine activity.
- Author
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Ledford JG, Lo B, Kislan MM, Thomas JM, Evans K, Cain DW, Kraft M, Williams KL, and Wright JR
- Subjects
- Animals, Blotting, Western, Cell Differentiation immunology, Cell Separation, Dendritic Cells cytology, Dendritic Cells metabolism, Flow Cytometry, HMGB1 Protein metabolism, Mice, Mice, Inbred C57BL, Mice, Mutant Strains, Mycoplasma Infections metabolism, Mycoplasma pneumoniae immunology, Pulmonary Surfactant-Associated Protein A metabolism, Dendritic Cells immunology, HMGB1 Protein immunology, Mycoplasma Infections immunology, Pulmonary Surfactant-Associated Protein A immunology
- Abstract
During pulmonary infections, a careful balance between activation of protective host defense mechanisms and potentially injurious inflammatory processes must be maintained. Surfactant protein A (SP-A) is an immune modulator that increases pathogen uptake and clearance by phagocytes while minimizing lung inflammation by limiting dendritic cell (DC) and T cell activation. Recent publications have shown that SP-A binds to and is bacteriostatic for Mycoplasma pneumoniae in vitro. In vivo, SP-A aids in maintenance of airway homeostasis during M. pneumoniae pulmonary infection by preventing an overzealous proinflammatory response mediated by TNF-α. Although SP-A was shown to inhibit maturation of DCs in vitro, the consequence of DC/SP-A interactions in vivo has not been elucidated. In this article, we show that the absence of SP-A during M. pneumoniae infection leads to increased numbers of mature DCs in the lung and draining lymph nodes during the acute phase of infection and, consequently, increased numbers of activated T and B cells during the course of infection. The findings that glycyrrhizin, a specific inhibitor of extracellular high-mobility group box-1 (HMGB-1) abrogated this effect and that SP-A inhibits HMGB-1 release from immune cells suggest that SP-A inhibits M. pneumoniae-induced DC maturation by regulating HMGB-1 cytokine activity.
- Published
- 2010
- Full Text
- View/download PDF
45. Solitary fibrous tumors of the soft tissues: review of the imaging and clinical features with histopathologic correlation.
- Author
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Wignall OJ, Moskovic EC, Thway K, and Thomas JM
- Subjects
- Adult, Aged, Aged, 80 and over, Angiography, Digital Subtraction, Biopsy, Diagnosis, Differential, Female, Humans, Magnetic Resonance Imaging methods, Male, Middle Aged, Neoplasms, Fibrous Tissue pathology, Neovascularization, Pathologic diagnosis, Neovascularization, Pathologic pathology, Prognosis, Soft Tissue Neoplasms pathology, Tomography, X-Ray Computed methods, Ultrasonography methods, Neoplasms, Fibrous Tissue diagnosis, Soft Tissue Neoplasms diagnosis
- Abstract
Objective: Solitary fibrous tumors are rare soft-tissue tumors of submesothelial origin with variable malignant potential. Most of these tumors originate within the thoracic cavity, but they can occur in a variety of sites, including the abdomen, pelvis, and soft tissues and muscles. The purpose of this study was to review the imaging findings with clinicopathologic correlation in 34 cases., Conclusion: The finding of a large, solid, vascular tumor, particularly with prominent feeding vessels or a visible fatty component, should alert the radiologist to the possible diagnosis of solitary fibrous tumor. Percutaneous biopsy carries minimal risk and should be used for definitive diagnosis of these lesions, which in many cases are curable with surgery. The prognosis is good for patients with benign tumors but variable for those with malignant tumors.
- Published
- 2010
- Full Text
- View/download PDF
46. Analysis of a rapid, simple, and inexpensive technique used to obtain platelet-rich plasma for use in clinical practice.
- Author
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Rutkowski JL, Thomas JM, Bering CL, Speicher JL, Radio NM, Smith DM, and Johnson DA
- Subjects
- Blood Platelets, Cell Shape, Centrifugation methods, Growth Substances analysis, Humans, Cell Separation methods, Platelet-Rich Plasma
- Abstract
The use of platelet-rich plasma (PRP) has become more generally accepted, and implant dentists are using PRP more frequently to promote the healing of oral surgical and/or periodontal wounds. Critical elements of PRP are thought to be growth factors contained within the concentrated platelets. These growth factors are known to promote soft-tissue healing, angiogenesis and osteogenesis. We present a rapid, simple, and inexpensive methodology for preparing PRP using the Cliniseal centrifuge method. This study demonstrates that platelets are concentrated approximately 6-fold without altering platelet morphology. Further we demonstrate that key growth factors, platelet-derived growth factor BB (PDGF-BB), transforming growth factor B (TGF-B1), vasculature endothelial growth factor (VEGF), and epidermal growth factor (EGF) are present in comparable or higher concentrations than those reported with the use of other techniques. Prolonged bench set time (>3 hours) after centrifugation resulted in decreased concentration of TGF-B1 but not decreased concentration of PDGF-BB, VEGF, or EGF. This study confirms the molecular aspects of PRP obtained using this inexpensive and efficient methodology.
- Published
- 2008
- Full Text
- View/download PDF
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