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While observing the difficulties that parents experience in communicating with their adolescent children (for example, ignoring, misunderstanding, or underestimating the problems faced by teenagers, inability to build trust-based relationships, difficulties in discussing complex topics of interpersonal relationships, life choices, school problems, self-determination, etc.), the authors emphasized the need to search for methodological tools that can help overcome these problems of intergenerational communication through developing the culture of experience among parents. A film club for parents was chosen as a method. 30 parents of children (4–20 years old) watched a German TV series DRUCK (based on a Norwegian TV series SKAM) in Moscow from January to December 2020 offline and online. The functioning of this club was built over the methods of polylogue, non-judgmental interview, and amplification of sense. Observations, questionnaires, and interviews of the club members at the end of meetings allowed the authors to conclude the effectiveness of such a format for developing the culture of experience among parents and improving intergenerational relations. It was also proved that teen series have a great potential for developing the culture of experience among parents since they address topics relevant for teens and help observe the emotions and behavior of teens in a psychologically safe environment. Based on the study results, the authors developed methodological recommendations for organizing such film clubs and holding meetings for parents.

The professional training of a clinical speech therapist traditionally focuses on the formation of professional competencies, motivation, and the ability to develop professionally, while the problems of coping with chronic emotional stress are common causes of professional deformations in them. It has been suggested that a high level of speech therapist’s readiness for professional activities, along with the individual typological characteristics of the personality described in the professiogram, is positively associated with a number of psychophysiological characteristics. An empirical study of personality traits, psychoemotional state, psychophysiological reactions, the adaptability of speech therapists (N = 56) was carried out using the psychophysiological testing device “UPFT-1/30 Psychophysiologist” (“Medicom MTD”). For the survey of teachers of three Russian state universities (N = 37), providing education clinical speech and language therapists were used the methods of analytical professiogramme E. M. Ivanova.

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Lead is one of the most dangerous xenobiotic elements. It is actively accumulated not only in the environment, the distribution of lead in the water-soil-plant-animal-man chain directly depends on the initial content of elements in the geochemical province and is directly related to the health of the population. [1]. This paper presents the results of a study of the biotoxicity of lead cations in the structure of salts with different anionic components in relation to bacteria of the genus Bacillus, which are part of the probiotic preparations. These microorganisms are representatives of the soil microflora and are transitory to the organism of animals and humans. When conducting research, methods such as the agar wells were used, which not only visually but also qualitatively evaluate the effect of metal cations on the growth of the microorganisms studied, the colorimetric method was used to assess the effect of the element under study on the growth of the microorganism population, and the atomic absorption method made it possible to study accumulating characteristics of the studied bacteria. As a result of research, it has been established that lead acetate has a more pronounced toxic effect on the microorganisms under study. It should be noted that lead, which is present in the medium in high concentrations, does not have an inhibitory effect on bacterial strains; we associate this with the detoxification mechanisms of bacteria. The accumulating ability of microorganisms of the Bacillus genus of lead cations from nutrient substrates 24 hours after their cultivation in the presence of this element has high sorption characteristics with a percentage of its accumulation of more than 50%, the most active lead accumulates B. subtilis 534 with 66.3%, and the minimum values were recorded in B. amyloliquefaciens 10642 and amounted to 53.2%. [ABSTRACT FROM AUTHOR]

This article analyzes positions of Russia in world tourist space on the basis of the tourism and travel competitiveness rating provided by the World Economic Forum. At the heart of rating lies The Travel and Tourism Competitiveness Index (TTCI) calculated on the basis of fourteen indicators grouped in four subindexes. On the basis of estimates of the indicators creating the index both the positive and negative influencing factors are allocated for a position of Russia in rating and The Travel and Tourism Competitiveness Index. It is noted that despite ambiguity of the estimates provided by the World Economic Forum, only complex assessment of the Russian tourism tendencies will allow defining new trends in its development and new mechanisms of increase in competitiveness of Russia in global tourist space. [ABSTRACT FROM AUTHOR]

V2O5 powders modified with different theoretical silver contents (1, 5, 10, 15, and 20 wt% as Ag2O) were obtained with acicular morphologies observed by scanning electron microscopy (SEM). Shcherbinaite crystalline phase is transformed into the Ag0.33V2O5 crystalline one with the incorporation and increase in silver content as was suggested by X-ray diffraction (XRD) and X-ray photoelectron spectroscopy (XPS) analysis. With further increase in silver contents the Ag2O phase appears. Catalysts were active in photocatalytic degradation of malachite green dye under simulated solar light, which is one of the most remarkable facts of this work. It was found that V2O5-20Ag was the most active catalytic formulation and its activity was attributed to the mixture of coupled semiconductors that promotes the slight decrease in the rate of the electron-hole pair recombination. [ABSTRACT FROM AUTHOR]

Carotenoids are the precursors for vitamin A and are proposed to prevent oxidative damage to cells. Mammalian genomes encode a family of structurally related nonheme iron oxygenases that modify double bonds of these compounds by oxidative cleavage and cis-to-trans isomerization. The roles of the family members BCMO1 and RPE65 for vitamin A production and vision have been well established. Surprisingly, we found that the third family member, β,β-carotene-9',10'-oxygenase (BCDO2), is a mitochondrial carotenoid-oxygenase with broad substrate specificity. In BCDO2-deficient mice, carotenoid homeostasis was abrogated, and carotenoids accumulated in several tissues. In hepatic mitochondria, accumulated carotenoids induced key markers of mitochondrial dysfunction, such as manganese superoxide dismutase (9-fold), and reduced rates of ADP-dependent respiration by 30%. This impairment was associated with an 8- to 9-fold induction of phosphor-MAP kinase and phosphor-AKT, markers of cell signaling pathways related to oxidative stress and disease. Administration of carotenoids to human HepG2 cells depolarized mitochondrial membranes and resulted in the production of reactive oxygen species. Thus, our studies in BCDO2-deficient mice and human cell cultures indicate that carotenoids can impair respiration and induce oxidative stress. Mammalian cells thus express a mitochondrial carotenoid-oxygenase that degrades carotenoids to protect these vital organelles. [ABSTRACT FROM AUTHOR]

Senescence is a potential tumor-suppressing mechanism and a commonly used model of cellular aging. One current hypothesis to explain senescence, based in part on the correlation of oxygen with senescence, postulates that it is caused by oxidative damage from reactive oxygen species (ROS). Here, we further test this theory by determining the mechanisms of hyperoxia-induced senescence. Exposure to 70% O2 led to stress-induced, telomere-independent senescence. Although hyperoxia elevated mitochondrial ROS production, overexpression of antioxidant proteins was not sufficient to prevent hyperoxia-induced senescence. Hyperoxia activated AMPK; however, overexpression of a kinase-dead mutant of LKB1, which prevented AMPK activation, did not prevent hyperexia-induced senescence. Knocking down p21 via shRNA, or suppression of the p16/pRb pathway by either BMI1 or HPV16-E7 overexpression, was also insufficient to prevent hyperoxia-induced senescence. However, suppressing p53 function resulted in partial rescue from senescence, suggesting that hyperexia-induced senescence involves p53. Suppressing both the p53 and pRb pathways resulted in almost complete protection, indicating that both pathways cooperate in hyperoxia-induced senescence. Collectively, these results indicate a ROS-independent but p53/pRb-dependent senescence mechanism during hyperoxia. [ABSTRACT FROM AUTHOR]

Mammalian cells increase transcription of genes for adaptation to hypoxia through the stabilization of hypoxia-inducible factor 1α (HIF-1α) protein. How cells transduce hypoxic signals to stabilize the HIF-1α protein remains unresolved. We demonstrate that cells deficient in the complex III subunit cytochrome b, which are respiratory incompetent, increase ROS levels and stabilize the HIF-1α protein during hypoxia. RNA interference of the complex III subunit Rieske iron sulfur protein in the cytochrome b-null cells and treatment of wild-type cells with stigmatellin abolished reactive oxygen species (ROS) generation at the Qo site of complex III. These interventions maintained hydroxylation of HIF-1α protein and prevented stabilization of HIF-1α protein during hypoxia. Antioxidants maintained hydroxylation of HIF-1α protein and prevented stabilization of HIF-1α protein during hypoxia. Exogenous hydrogen peroxide under normoxia prevented hydroxylation of HIF-1α protein and stabilized HIF-1α protein. These results provide genetic and pharmacologic evidence that the Qo site of complex III is required for the transduction of hypoxic signal by releasing ROS to stabilize the HIF-1α protein. [ABSTRACT FROM AUTHOR]

2,3,7,8-Tetrachlorodibenzo-p-dioxin (TCDD) is a potent developmental toxicant in most vertebrates. However, frogs are relatively insensitive to TCDD toxicity, especially during early life stages. Toxicity of TCDD and related halogenated aromatic hydrocarbons is mediated by the aryl hydrocarbon receptor (AhR), and specific differences in properties of the AhR signaling pathway can underlie in TCDD toxicity in different species. This study investigated the role of AhR in frog TCDD insensitivity, using Xenopus laevis as a model system. X. laevis, a pseudotetraploid species, expresses two distinct AhR1 genes, AhR1α and AhR1β. Sharing 86% amino acid identity, these likely represent distinct genes, both orthologous to mammalian AhR and paralogous to the AhR2 gene(s) in most fish. Both AhR1α and AhR1β exhibit TCDD-dependent binding of cognate DNA sequences, but they bind TCDD with at least 20-fold lower affinity than the mouse AhRb–1 protein, and they are similarly less responsive in TCDD-induced reporter gene induction in conjunction with the mouse CYP1A1 promoter. Furthermore, CYP1A6 and CYP1A7 induction by TCDD in cultured X. laevis A6 cells appears much less responsive than CYP1A induction in cell lines derived from more sensitive animals. Taken together, these data suggest that low affinity binding by X. laevis AhRs plays an important mechanistic role in the insensitivity of frogs to TCDD. An understanding of these molecular mechanisms should aid amphibian ecotoxicology and refine the use of frog embryos as a model [e.g. in FETAX (Frog Embryo Teratogenesis Assay-Xenopus)] for determining developmental toxicity of samples containing dioxin-like compounds. [ABSTRACT FROM AUTHOR]

The aim of our study was to determine the possibility of using ultrafine Fe particles (2.2 mg/head/day) as modulators of metabolic activity when using vegetable soybean and sunflower oils in ruminant diets. The studies carried out on calves with external duodenal anastomosis (diet - grassland hay (2 kg), a mixture of concentrates (1.5 kg), corn silage (5 kg), wheat straw (1 kg), oil-3% of the dry matter of the diet). The digestibility of the nutritional components of the diet analyzed daily throughout the study period. The collection of pancreatic juice was carried out for 8 hours with an interval of 60 minutes on the 7th day, the determination of the amount and enzymatic activity of the juice carried out "incito." Introduction of ultrafine Fe particles significantly increased the digestibility of organic matter by 38% (P = 0.05) with the introduction of vegetable soybean oil, crude fat by 28.4% (P = 0.05) with the introduction of sunflower oil. Additional introduction of ultrafine Fe particles significantly increased the level of pancreatic secretion when sunflower oil was introduced by 59.8%, soybean oil by 56% (P = 0.05). Additional inclusion of ultrafine iron particles contributed to the decrease of lipase, protease and amylase activity by 86,8%, 37,4% and 33% (P = 0,05) in the sunflower oil group, by 68,2% of lipase, by 24,6% of amylase in the soybean oil group (P = 0,05). A significant increase in the level of NO-metabolites was recorded in the group receiving soybean oil by 37.5 % (P < 0.05). This study shows the potential use of ultrafine Fe particles as a mineral supplement to regulate the functional activity of digestive and metabolic processes in ruminants, which may play a positive role in increasing the bioavailability of nutritional components of diets. This research performed with financial support from the project 0761-2019-0005. [ABSTRACT FROM AUTHOR]

Physiological hypoxia extends the replicative life span of human cells in culture. Here, we report that hypoxic extension of replicative life span is associated with an increase in mitochondrial reactive oxygen species (ROS) in primary human lung fibroblasts. The generation of mitochondrial ROS is necessary for hypoxic activation of the transcription factor hypoxia-inducible factor (HIF). The hypoxic extension of replicative life span is ablated by a dominant negative HIF. HIF is sufficient to induce telomerase reverse transcriptase mRNA and telomerase activity and to extend replicative life span. Furthermore, the down-regulation of the von Hippel-Lindau tumor suppressor protein by RNA interference increases HIF activity and extends replicative life span under normoxia. These findings provide genetic evidence that hypoxia utilizes mitochondrial ROS as signaling molecules to activate HIF-dependent extension of replicative life span. [ABSTRACT FROM AUTHOR]

As tumors develop, they outgrow the vascular network that supplies cells with oxygen and nutrients needed for survival. In response to decreased oxygen levels, the tumor cells initiate a program of adaptation by inducing the transcription of multiple genes via the activation of the transcription factor hypoxia-inducible factor (HIF). Proteins encoded by a subset of genes induced by HIF promote tumorigenesis by acting directly on both the tumor cells and the microenvironment in which the tumor cells reside. The mechanism(s) by which hypoxia activates HIF is a subject of intensive research. Understanding how hypoxia activates HIF will provide targets for the development of therapies that could specifically target growing tumors by not allowing adequate adaptation to hypoxia, which is necessary for cancer progression. Here we outline how mitochondria regulate the activity of HIF during hypoxia.

Mammalian cells increase transcription of genes for adaptation to hypoxia through the stabilization of hypoxia-inducible factor 1alpha (HIF-1alpha) protein. How cells transduce hypoxic signals to stabilize the HIF-1alpha protein remains unresolved. We demonstrate that cells deficient in the complex III subunit cytochrome b, which are respiratory incompetent, increase ROS levels and stabilize the HIF-1alpha protein during hypoxia. RNA interference of the complex III subunit Rieske iron sulfur protein in the cytochrome b-null cells and treatment of wild-type cells with stigmatellin abolished reactive oxygen species (ROS) generation at the Qo site of complex III. These interventions maintained hydroxylation of HIF-1alpha protein and prevented stabilization of HIF-1alpha protein during hypoxia. Antioxidants maintained hydroxylation of HIF-1alpha protein and prevented stabilization of HIF-1alpha protein during hypoxia. Exogenous hydrogen peroxide under normoxia prevented hydroxylation of HIF-1alpha protein and stabilized HIF-1alpha protein. These results provide genetic and pharmacologic evidence that the Qo site of complex III is required for the transduction of hypoxic signal by releasing ROS to stabilize the HIF-1alpha protein.