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Feature selection is a fundamental pre‐processing step in machine learning that aims to reduce the dimensionality of a dataset by selecting the most effective features from the original features. This process is regarded as a combinatorial optimization problem, and the grey wolf optimizer (GWO), a novel meta‐heuristic algorithm, has gained popularity in feature selection due to its fast convergence speed and easy implementation. In this paper, an improved binary GWO algorithm incorporating a novel Population Adaptation strategy called PA‐BGWO is proposed. The PA‐BGWO takes into account the characteristics of the feature selection problem and designs three strategies. The proposed strategy includes an adaptive individual update procedure to enhance the exploitation ability and accelerate convergence speed, a head wolf fine‐tuned mechanism to exert the impact on each independent feature of the objective function, and a filter‐based method ReliefF for calculating feature weights with dynamically adjusted mutation probabilities based on the ranking features to effectively escape from local optima. Experimental comparisons with several state‐of‐the‐art feature selection methods on 15 classification problems demonstrate that the proposed approach can select a small feature subset with higher classification accuracy in most cases. [ABSTRACT FROM AUTHOR]

The morbidity and mortality of cardiovascular diseases (CVDs) have been increasing year by year all over the world and expanding greatly to the younger population, which becomes the leading causes of death globally that threatens human life safety. Prediction of the occurrence of diseases by using risk related adverse events is crucial for screening and early detection of CVDs. Thus, the discovery of new biomarkers that related to risks of CVDs are of urgent in the field. Retinol-binding protein 4 (RBP4) is a 21-kDa adipokine, mainly secreted by adipocytes. Besides its well-established function in the induction of insulin resistance, it has also been found in recent years to be closely associated with CVDs and other risk factors, such as hypertension, coronary heart disease, heart failure, obesity, and hyperlipidemia. In this review, we mainly focus on the progress of research that establishes the correlation between RBP4 and CVDs and the corresponding major risk factors in recent years. [ABSTRACT FROM AUTHOR]

Electrospun polymer/metal composite nanofibers have received much attention in the field of bone tissue engineering and regenerative medicine (BTERM) owing to their extracellular matrix- (ECM-) like structure, sufficient mechanical strength, favorable biological properties, and bone induction. In particular, electrospun nanofibers containing strontium (Sr) can significantly promote bone repair and regeneration by mediating osteolysis and osteogenesis, which offers a promising bioactive material for BTERM. In this review, we summarized the effects of electrospun nanofibers containing Sr on stem cells, osteoblasts, and osteoclasts in BTERM. Also, current challenges and future perspectives for electrospun nanofibers containing Sr in BTERM are briefly outlined. It is hoped that the systematic overview will inspire the readers to further study Sr-containing nanofibers for BTERM and accelerate their translation from the bench to the clinic. [ABSTRACT FROM AUTHOR]

Chitosan and its derivatives have been increasingly used for bacteriostasis. To date, the effect of chitosan and N-(2-hydroxyl) propyl-3-trimethyl ammonium chitosan chloride (HTCC) on Enterococcus faecalis (E. faecalis) associated with endodontic infection has remained to be determined. Chitosan and HTCC were serially diluted with double-distilled water (DDW) or PBS at concentrations of 20-2,500 µg/ml. Various strains of E. faecalis (American Type Tissue Collection no. 29212, as well as isolated strains P25RC and P52Sa) in plankton were adjusted to an optical density at 600 nm of 0.10 and treated with chitosan or HTCC. A colony-forming unit assay was used to determine the concentration of residual bacteria after treatment. Furthermore, E. faecalis biofilms were cultured on coverslips and treated with chitosan or HTCC. The coverslips were rinsed, stained using Live/dead® BacLight™ bacterial viability kit and observed under an inverted fluorescence microscope. In addition, biofilms on dentine blocks were prepared and observed under a scanning electron microscope. MC3T3-E1 pre-osteoblasts were seeded on 96-well plates and treated with chitosan or HTCC at various concentrations. The cytotoxicity of chitosan and HTCC on MC3T3-E1 pre-osteoblasts was detected using a Cell Counting Kit-8 assay after 24, 48 and 72 h of treatment. The results revealed that the final minimum bactericidal concentrations (MBC) of chitosan and HTCC dissolved in DDW were 70 and 140 µg/ml, respectively. Chitosan and HTCC in DDW exerted a significantly greater antibacterial effect as compared with that in PBS (P<0.05). At the MBC, chitosan and HTCC in DDW, but particularly chitosan, had a significant antibacterial effect on E. faecalis biofilm. Chitosan exhibited no cytotoxicity to MC3T3-E1 pre-osteoblasts at a concentration of <625 µg/ml, while HTCC inhibited the proliferation of the cells in the concentration range of 39-10,000 µg/ml. In conclusion, chitosan and HTCC exhibited prominent antibacterial properties on E. faecalis in the planktonic state and as a biofilm via charge interaction, indicating their potential for application in root canal disinfection and fillings. [ABSTRACT FROM AUTHOR]

[Display omitted] • All organic PC 71 BM/g-C 3 N 4 photocatalysts were constructed by in situ self-assembly. • The heterostructures possess satisfactory biocompatibility. • The PC 71 BM served as the electron acceptor and photosensitizer. • · O 2 - acted as the main active species in the degradation process. Photocatalysis has been reported as a potential water purification technology for combating the pathogenic bacteria and degrading the hazardous chemicals. The photoactive organic semiconductors as the emerging class of an alternative to the traditional developed inorganic semiconductors are being widely pursued due to their tunable structures and low toxicity. Herein, the all-organic [6,6] -phenyl C 71 butyric acid methyl ester (PC 71 BM)/g-C 3 N 4 heterostructure have been created by introducing self-assembled n-type fullerene small molecule to the g-C 3 N 4 surface in situ. The absorption edge of the heterostructures with PC 71 BM as the photosensitizer extend from 450 to 650 nm. Moreover, the charge separation and transfer efficiencies of the photocatalysts are greatly promoted even with 1.0% amount of PC 71 BM because of its low reorganization energy and high reduction potential. All of these superiorities endow the heterostructures with over 99.7% antibacterial rates toward E. coli and S. aureus and excellent performance against their biofilms after irradiation for 135 and 180 min, respectively. Additionally, the heterostructures achieve excellent photocatalytic degradation ability for Rhodamine B and tetracycline. Importantly, the in vitro experiments reveal that the as-prepared heterostructures possess satisfactory biocompatibility and their toxicity is negligible. This study provides a new perspective for designing ecofriendly all-organic heterostructures with high photocatalytic activity. [ABSTRACT FROM AUTHOR]

Fucoidan has received much attention in healthy food and biomedicine owing to their unique (bio)physicochemical properties, particularly antibacterial and antiviral. Pathogenic microorganisms and probiotics are coexisting in many tissues (e.g., gut, oral, and vagina). However, the effect of fucoidan on probiotics has not been examined. Herein, fucoidan sterilized by different methods (i.e., 0.22 μm filter and high-temperature autoclave) is applied to explore its effect on the responses of Lactobacillus rhamnosus. It is found that high-temperature autoclave treatment causes the depolymerization of fucoidan. Further, the proliferation, morphology, and metabolism of probiotics are greatly dependent on the concentrations of fucoidan. The formation of probiotic biofilm is reduced with an increased concentration of fucoidan. Moreover, the antibacterial ability of probiotics initially increases and then decreases with an increased concentration of fucoidan. Thus, fucoidan could serve as a new marine-origin prebiotic, offering new insight into probiotic modulation and its application in inhibiting bacterial infections. • Depolymerized fucoidan with appropriate concentration promotes the growth of L. rhamnosus. • Depolymerized fucoidan affects the morphology and metabolism of L. rhamnosus. • Depolymerized fucoidan inhibits the formation of probiotic biofilm. • Depolymerized fucoidan enhances the antibacterial ability of L. rhamnosus. • Depolymerized fucoidan serves as a marine-origin prebiotic. [ABSTRACT FROM AUTHOR]

Conventional wound-dressing materials with structural and functional deficiencies are not effective in promoting wound healing. The development of multifunctional wound dressings is emerging as a promising strategy to accelerate blood coagulation, inhibit bacterial infection, and trigger full-thickness wound into a regenerative process. Herein, multifunctional composite sponges were developed by incorporation of traditional Chinese medicine Kangfuxin (KFX) into alginate (AG)/carboxymethyl chitosan (CMC) via green crosslinking, electrostatic interaction, and freeze-drying methods. It is demonstrated that the AG/CMC/KFX (ACK) sponges exhibit a highly interconnected and porous structure, suitable water vapor transmittance, excellent elastic properties, antibacterial behavior, cytocompatibility, and rapid hemostasis. Further, in a rat full-thickness wounds model, the ACK sponge containing 10% KFX (ACK-10) significantly facilitates wound closure compared to the AC group and ACK sponge containing 5% and 15% KFX. Thus, the multifunctional ACK-10 composite sponge has great promise for the application of full-thickness wound healing. Unlabelled Image • Multifunctional alginate/carboxymethyl chitosan/kangfuxin sponges (ACK) were successfully fabricated. • ACK possessed a highly porous structure and excellent elastic properties. • ACK accelerated hemostasis and inhibited bacterial infection • ACK triggered the full-thickness wound into a regenerative process. [ABSTRACT FROM AUTHOR]

Background: Existing research has shown that retinol binding protein (RBP4) has an impairing effect on arterial elasticity and induces insulin resistance, but the clinical value of RBP4 in patients with coronary heart disease (CHD) combined with type 2 diabetes mellitus (T2DM) has not been investigated. This study sought to compare the complexity of coronary artery lesions and coronary artery elasticity between patients with CHD combined with T2DM and those with CHD without T2DM, analyze the risk factors affecting coronary artery elasticity, and investigate the value of RBP4 in assessing coronary artery elasticity in patients with CHD and T2DM., Methods: A total of 130 patients with confirmed CHD were consecutively enrolled, including 38 patients with CHD combined with T2DM and 92 patients with CHD without T2DM. Basic clinical data, laboratory findings, coronary angiography and intravascular ultrasound (IVUS) imaging data, and Gensini scores and coronary artery elasticity parameters were calculated in both groups. Elasticity parameters included: stiffness parameter ( β ), pressure-strain elastic modulus ( E p ), distensibility coefficient (DC), and compliance coefficient (CC). Multiple linear regression equations were established with elasticity parameters as dependent variables to explore the factors influencing coronary artery elasticity parameters in patients within the two groups., Results: Compared with patients in the CHD without T2DM group, patients in the CHD combined with T2DM group had higher RBP4 levels, Gensini scores, β and E p values, and lower DC and CC values. Linear regression analysis showed that Gensini score increased with higher β and E p values and decreased with higher DC and CC values. In all patients in the CHD and CHD combined with T2DM groups, RBP4 was an independent risk factor for β values after correction for confounders by multiple linear regression analysis, whereas in patients in the CHD without T2DM group, the effect of RBP4 on β values was not statistically different., Conclusions: RBP4 was an independent risk factor of coronary artery elasticity in CHD patients with T2DM and in overall CHD patients, but it did not affect coronary artery elasticity in CHD patients without T2DM., Competing Interests: The authors declare no conflict of interest., (Copyright: © 2023 The Author(s). Published by IMR Press.)

Soft-tissue trauma emergency caused by natural disasters and traffic accidents is highly prevalent, which can result in massive bleeding, pathogen infection, and even death. Although numerous tissue adhesives can bind to tissue surfaces and cover wounds, most of them still have several deficiencies, including long gelation time, poor adhesive strength, and anti-infection, making them inappropriate for use as first-aid bandages. Herein, injectable and self-healing four-arm-PEG-CHO/polyethyleneimine (PEI) tissue adhesives as liquid first-aid supplies are developed via the dynamic Schiff base reaction for trauma emergency. It is found that the prepared hydrogel adhesives exhibit short and controlled gelation time (9∼88 s), strong adhesive strength, and excellent antibacterial ability. Their hemostatic and antimicrobial performances can be tailored by the mass ratio of four-arm-PEG-CHO/PEI. Moreover, in vitro biological assays display that the developed tissue adhesives possess satisfactory cyto/hemocompatibility. Importantly, in vivo the designed adhesives show fast hemostatic capacity and excellent anti-infection as compared to commercial Prontosan gel. Thus, this work indicates that the four-arm-PEG-CHO/PEI first-aid tissue adhesives display great potential for wound emergency management.

Background: Retinol binding protein 4 (RBP4), a biomarker for insulin resistance in type 2 diabetes (DM), is increased in heart failure. This case-control study aims to determine the association between serum RBP4 levels and diabetic cardiomyopathy (DCM)., Methods: Demographic and clinical data were obtained from 245 DM patients and 102 non-diabetic controls. RBP4 levels were measured using ELISA. The association between RBP4 and DCM was evaluated using multivariate logistic regression and restricted cubic splines (RCS) in DM patients., Results: We showed that serum RBP4 levels were higher in DCM patients than in DM patients without DCM or the controls. Multivariate analysis adjusted by age, gender, body mass index, diabetes duration, left ventricular ejection fraction, insulin treatment, triglycerides, low-density lipoprotein cholesterol, estimated glomerular filtration rate, diabetic retinopathy, diabetic nephropathy, diabetic neuropathy and log N-terminal proBNP showed a significant association between RBP4 and DCM (highest vs. lowest tertile OR 16.87, 95% CI: 6.58, 43.23, p < 0.001). RCS displayed a positive linear correlation between RBP4 levels and the risk of DCM in diabetes ( p = 0.004). Adding RBP4 to a basic risk model for DCM improved the reclassification (Net reclassification index: 87.86%, 95% CI: 64.4%, 111.32%, p < 0.001)., Conclusions: The positive association between serum RBP4 and DCM suggested the role of RBP4 as a potential diagnostic biomarker for distinguishing DCM in patients with DM., Competing Interests: The authors declare no conflict of interest., (Copyright: © 2022 The Author(s). Published by IMR Press.)