1. [2-hydroxy-methyl-1(N-phtaloyltryptophyl) aziridine stimulates in vitro human lymphocyte proliferation and interleukin secretion].
- Author
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Baba Ahmed FZ, Bouanane S, Merzouk SA, Merzouk H, Medjahed W, Kajima Mulengi J, and Prost J
- Subjects
- B-Lymphocytes drug effects, B-Lymphocytes immunology, Cell Separation methods, Humans, Interleukin-2 blood, Interleukin-5 blood, Lymphocytes drug effects, T-Lymphocytes drug effects, T-Lymphocytes immunology, Tryptophan pharmacology, Aziridines pharmacology, Interleukin-2 metabolism, Interleukin-5 metabolism, Lymphocyte Activation drug effects, Tryptophan analogs & derivatives
- Abstract
Unlabelled: AIM OF THIS WORK: Aziridines have been shown to possess marked immunotropic activity. In this study, the in vitro effects of a new aziridine, 2-hydroxy-methyl-1-(N-phtaloyltryptophyl) aziridine, were determined on the proliferative responses of human lymphocytes stimulated by mitogens and on interleukin (IL-2, IL-6) secretion., Material and Methods: Peripheral blood lymphocytes were isolated using differential centrifugation on a density gradient of Ficoll-Paque. They were cultured with or without mitogens (Concanavalin A and lipopolysaccharide), and with different concentrations of the aziridine. Proliferation was monitored by direct cell counts and confirmed by MTT [3-(4,5-dimethyl thiazol-2-yl)-2,5-diphenyl tetrazolium bromide] assay. After different incubations, IL-2 and IL-6 were determined by using commercially available Elisa kits., Results: The aziridine tested significantly stimulated the resting and mitogen T and B lymphocyte proliferation at concentrations between 1 microM and 1 mM, in a dose-dependent manner. It also increased IL-2 and IL-6 secretion., Conclusion: 2-hydroxy-methyl-1-(N-phtaloyltryptophyl) aziridine displayed immunomodulatory properties and is potentially immunostimulant. It could be used to provide non-specific cell-mediated immune responses.
- Published
- 2008
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