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[2-hydroxy-methyl-1(N-phtaloyltryptophyl) aziridine stimulates in vitro human lymphocyte proliferation and interleukin secretion].
- Source :
-
Pathologie-biologie [Pathol Biol (Paris)] 2008 May; Vol. 56 (3), pp. 137-42. Date of Electronic Publication: 2007 Jul 03. - Publication Year :
- 2008
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Abstract
- Unlabelled: AIM OF THIS WORK: Aziridines have been shown to possess marked immunotropic activity. In this study, the in vitro effects of a new aziridine, 2-hydroxy-methyl-1-(N-phtaloyltryptophyl) aziridine, were determined on the proliferative responses of human lymphocytes stimulated by mitogens and on interleukin (IL-2, IL-6) secretion.<br />Material and Methods: Peripheral blood lymphocytes were isolated using differential centrifugation on a density gradient of Ficoll-Paque. They were cultured with or without mitogens (Concanavalin A and lipopolysaccharide), and with different concentrations of the aziridine. Proliferation was monitored by direct cell counts and confirmed by MTT [3-(4,5-dimethyl thiazol-2-yl)-2,5-diphenyl tetrazolium bromide] assay. After different incubations, IL-2 and IL-6 were determined by using commercially available Elisa kits.<br />Results: The aziridine tested significantly stimulated the resting and mitogen T and B lymphocyte proliferation at concentrations between 1 microM and 1 mM, in a dose-dependent manner. It also increased IL-2 and IL-6 secretion.<br />Conclusion: 2-hydroxy-methyl-1-(N-phtaloyltryptophyl) aziridine displayed immunomodulatory properties and is potentially immunostimulant. It could be used to provide non-specific cell-mediated immune responses.
- Subjects :
- B-Lymphocytes drug effects
B-Lymphocytes immunology
Cell Separation methods
Humans
Interleukin-2 blood
Interleukin-5 blood
Lymphocytes drug effects
T-Lymphocytes drug effects
T-Lymphocytes immunology
Tryptophan pharmacology
Aziridines pharmacology
Interleukin-2 metabolism
Interleukin-5 metabolism
Lymphocyte Activation drug effects
Tryptophan analogs & derivatives
Subjects
Details
- Language :
- French
- ISSN :
- 0369-8114
- Volume :
- 56
- Issue :
- 3
- Database :
- MEDLINE
- Journal :
- Pathologie-biologie
- Publication Type :
- Academic Journal
- Accession number :
- 17611043
- Full Text :
- https://doi.org/10.1016/j.patbio.2007.05.004