35 results on '"Huang, Chuanjiang"'
Search Results
2. Dihydroartemisinin inhibited vasculogenic mimicry in gastric cancer through the FGF2/FGFR1 signaling pathway
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Wang, Huina, Ding, Qingzhu, zhou, Haihua, Huang, Chuanjiang, Liu, Guiyuan, Zhao, Xiaojun, Cheng, Zhiyi, and You, Xiaolan
- Published
- 2024
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3. Identification and validation of oxidative stress and immune-related hub genes in Alzheimer’s disease through bioinformatics analysis
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Li, Shengjie, Xiao, Jinting, Huang, Chuanjiang, and Sun, Jikui
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- 2023
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4. Quantitatively study on wave-turbulence interactions by laboratory experiments
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Ma, Hongyu, Dai, Dejun, Jiang, Shumin, Huang, Chuanjiang, Deng, Jia, and Qiao, Fangli
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- 2022
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5. Noise reduction of acoustic Doppler velocimeter data based on Kalman filtering and autoregressive moving average models
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Huang, Chuanjiang, Qiao, Fangli, and Ma, Hongyu
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- 2020
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6. KLF5 activates lncRNA DANCR and inhibits cancer cell autophagy accelerating gastric cancer progression
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Cheng, Zhiyi, Liu, Guiyuan, Huang, Chuanjiang, and Zhao, Xiaojun
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- 2021
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7. Can Langmuir Circulations Solve the Problem of Insufficient Upper-Ocean Mixing?
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Huang, Chuanjiang, Qiao, Fangli, and Dai, Dejun
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- 2020
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8. Contribution of surface wave-induced vertical mixing to heat content in global upper ocean
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Chen, Siyu, Qiao, Fangli, Huang, Chuanjiang, and Song, Zhenya
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- 2020
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9. High versus low ligation of inferior mesenteric artery during laparoscopic radical resection of rectal cancer: A retrospective cohort study
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You, Xiaolan, Liu, Qinghong, Wu, Jian, Wang, Yuanjie, Huang, Chuanjiang, cao, Gan, Dai, Jiawen, Chen, Dehu, and Zhou, Yan
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- 2020
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10. Sea level rise projection in the South China Sea from CMIP5 models
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Huang, Chuanjiang and Qiao, Fangli
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- 2015
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11. Efficacy of DC-CIK Immunotherapy Combined with Chemotherapy on Locally Advanced Gastric Cancer.
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Liu, Guiyuan, Chen, Dehu, Zhao, Xiaojun, You, Xiaolan, Huang, Chuanjiang, Cheng, Zhiyi, Mao, Xunan, and Zhou, Haihua
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STOMACH cancer ,KILLER cells ,T cells ,IMMUNOTHERAPY ,APPETITE loss ,IRRITABLE colon - Abstract
The aim of the study is to explore the efficacy and safety of dendritic cell-cytokine-induced killer cell (DC-CIK) immunotherapy combined with chemotherapy in the treatment of locally advanced gastric cancer (LAGC). Among 106 patients with LAGC, 53 received the treatment of oxaliplatin-5-fluorouracil chemotherapy (control group), while the remaining 53 received DC-CIK immunotherapy combined with chemotherapy (DC-CIK group). The short-term efficacy and the changes in immune function indexes (cluster of differentiation (CD)3
+ , CD4+ , CD8+ , CD4+ /CD8+ , and natural killer (NK) cells) were analyzed. The overall response rate (ORR) was 47.2% (25/53) and 41.5% (22/53), and the disease control rate (DCR) was 69.8% (37/53) and 50.9% (27/53), respectively, in the DC-CIK group and the control group. It could be seen that the ORR had no statistically significant difference between the two groups, while the DCR in the DC-CIK group was significantly better than that in the control group. After treatment, the proportions of CD3+ T lymphocytes, CD4+ T lymphocytes, CD4+ /CD8+ cells, and NK cells obviously rose, while the proportion of CD8+ T lymphocytes obviously declined in the DC-CIK group compared with those in the control group. After treatment, the scores in the function module of the QLQ-C30 scale were greatly higher in the DC-CIK group than those in the control group, while the scores of loss of appetite, constipation, dyspnea, fatigue, pain, and sleep disorders in the symptom module were significantly lower in the DC-CIK group than those in the control group. The median survival time was 23.4 months and 18.6 months, respectively, in the DC-CIK group and the control group. The results of the log-rank test showed that the OS in the DC-CIK group was remarkably superior to that in the control group. DC-CIK immunotherapy combined with chemotherapy can improve the immune cell function, ameliorate the quality of life, and prolong the survival time of LAGC patients, with fewer adverse reactions. [ABSTRACT FROM AUTHOR]- Published
- 2022
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12. Effects of the surface wave-induced mixing on circulation in an isopycnal-coordinate oceanic circulation model
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Huang, Chuanjiang, Qiao, Fangli, and Wei, Zexun
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- 2013
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13. Effects of horizontal mixing on the upper ocean temperature in the equatorial Pacific Ocean
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Huang, Chuanjiang and Qiao, Fangli
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- 2012
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14. Mid-Holocene seawater preserved in the deepest oceanic blue hole
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Qiao, Fangli, Huang, Chuanjiang, Li, Tiegang, Zhang, Min, Jiang, Wenzheng, and Liu, Yanxiong
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- 2020
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15. High versus low ligation of inferior mesenteric artery during laparoscopic radical resection of rectal cancer: A retrospective cohort study.
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Xiaolan You, Qinghong Liu, Jian Wu, Yuanjie Wang, Chuanjiang Huang, Gan cao, Jiawen Dai, Dehu Chen, Yan Zhou, You, Xiaolan, Liu, Qinghong, Wu, Jian, Wang, Yuanjie, Huang, Chuanjiang, Cao, Gan, Dai, Jiawen, Chen, Dehu, and Zhou, Yan
- Published
- 2020
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16. Overexpression of p53 delivered using recombinant NDV induces apoptosis in glioma cells by regulating the apoptotic signaling pathway.
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Fan, Xiaoyong, Lu, Hongzhen, Cui, Youqiang, Hou, Xianzeng, Huang, Chuanjiang, and Liu, Guangcun
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GLIOMAS ,APOPTOSIS ,GENE expression ,CELL lines ,JAK-STAT pathway - Abstract
Malignant glioma is the most common primary brain carcinoma in the world and has a poor survival rate. Previous studies have demonstrated that p53 dysfunction contributes to the development and severity of malignant glioma. It has also been demonstrated that Newcastle disease virus (NDV) may be a viable candidate for the treatment of various types of cancer. In the present study, a p53 oncolytic agent delivered using recombinant NDV (rNDV-p53) was constructed and its anti-tumor effects in vitro and in vivo were assessed. Glioma cell lines and a xenograft mouse model were utilized to assess the ability of p53 and rNDV to promote apoptosis and induce immunotherapy, respectively. The mechanism of rNDV-p53 in glioma therapy was investigated using quantitative polymerase chain reaction and immunohistochemistry. Tumor-specific cytotoxic T-lymphocyte (CTL) responses and lymphocyte infiltration were also analyzed in glioma-bearing models. The results of the present study demonstrate that rNDV-p53 may be a potential therapeutic agent that improves the prognosis of mice with glioma. It was revealed that rNDV-p53 inhibits glioma cell growth and aggressiveness in vitro and in vivo compared with rNDV and p53 alone. The results also demonstrated that rNDV-p53 induced glioma cell apoptosis by upregulating apoptosis-related genes. In addition, the present study demonstrated that rNDV-p53 significantly stimulated CTL responses and lymphocyte infiltration whilst increasing the number of apoptotic bodies in vivo. Furthermore, rNDV-p53 therapy inhibited tumor regression and prolonged the survival of glioma-bearing mice. In conclusion, rNDV-p53 invoked an immune response against glioma cells, which may serve as a comprehensive immunotherapeutic schedule for glioma. [ABSTRACT FROM AUTHOR]
- Published
- 2018
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17. Effects of the Non‐breaking Surface Wave‐induced Vertical Mixing on Winter Mixed Layer Depth in Subtropical Regions.
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Chen, Siyu, Qiao, Fangli, Huang, Chuanjiang, and Song, Zhenya
- Abstract
Abstract: Compared to observations, the simulated multi‐model mean surface oceanic mixed layer depth (MLD) during winter in the subtropical regions of both hemispheres shows deep bias from 45 CMIP5 climate models. Our results from two numerical experiments using one of CMIP5 models show that the non‐breaking surface wave‐induced vertical mixing can serve as a remedy. The enhanced vertical mixing increases the heat content of the upper ocean and reduces the oceanic potential density in winter which then stabilize the upper ocean and shallow the simulated winter MLD in subtropical regions. This heat content increase is not directly induced by air‐sea heat fluxes during winter, but is the legacy of temperature increase during summer, when the additional vertical mixing induces an enhanced surface heating. The simulation biases of the annually averaged water temperatures in the upper 400 m reduced by 43% and 28% in south and north latitude bands between 20°and 40°, respectively. The non‐breaking surface wave‐induced vertical mixing shallows both boreal and austral winter MLDs by 2–11 m (a change of 5–20%) in both northern and southern subtropical regions. These results indicate that the incorporation of vertical mixing induced by the non‐breaking surface waves in our experiments can improve the simulation of boreal and austral winter MLDs in northern and southern subtropical regions. [ABSTRACT FROM AUTHOR]
- Published
- 2018
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18. The relationship between sea surface temperature anomaly and wind energy input in the Pacific Ocean
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Huang, Chuanjiang and Qiao, Fangli
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- 2009
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19. Development and evaluation of an Earth System Model with surface gravity waves.
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Qiao, Fangli, Song, Zhenya, Bao, Ying, Song, Yajuan, Shu, Qi, Huang, Chuanjiang, and Zhao, Wei
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- 2013
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20. Shikonin Kills Glioma Cells through Necroptosis Mediated by RIP-1.
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Huang, Chuanjiang, Luo, Yinan, Zhao, Jingwei, Yang, Fuwei, Zhao, Hongwei, Fan, Wenhai, and Ge, Pengfei
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SHIKONIN , *GLIOMAS , *LEUKEMIA , *CANCER treatment , *ONCOLOGY , *OXIDATIVE stress , *CANCER chemotherapy - Abstract
Background and Purpose: Shikonin was reported to induce necroptosis in leukemia cells, but apoptosis in glioma cell lines. Thus, it is needed to clarify whether shikonin could cause necroptosis in glioma cells and investigate its underlying mechanisms. Methods: Shikonin and rat C6 glioma cell line and Human U87 glioma cell line were used in this study. The cellular viability was assayed by MTT. Flow cytometry with annexin V-FITC and PI double staining was used to analyze cellular death modes. Morphological alterations in C6 glioma cells treated with shikoinin were evaluated by electronic transmission microscopy and fluorescence microscopy with Hoechst 33342 and PI double staining. The level of reactive oxygen species was assessed by using redox-sensitive dye DCFH-DA. The expressional level of necroptosis associated protein RIP-1 was analyzed by western blotting. Results: Shikonin induced cell death in C6 and U87 glioma cells in a dose and time dependent manner. The cell death in C6 and U87 glioma cells could be inhibited by necroptosis inhibitor necrotatin-1, not by pan-caspase inhibitor z-VAD-fmk. Shikonin treated C6 glioma cells presented electron-lucent cytoplasm, loss of plasma membrane integrity and intact nuclear membrane in morphology. The increased ROS level caused by shikonin was attenuated by necrostatin-1 and blocking ROS by anti-oxidant NAC rescued shikonin-induced cell death in both C6 and U87 glioma cells. Moreover, the expressional level of RIP-1 was up-regulated by shikonin in a dose and time dependent manner as well, but NAC suppressed RIP-1 expression. Conclusions: We demonstrated that the cell death caused by shikonin in C6 and U87 glioma cells was mainly via necroptosis. Moreover, not only RIP-1 pathway, but also oxidative stress participated in the activation of shikonin induced necroptosis. [ABSTRACT FROM AUTHOR]
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- 2013
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21. Upregulation of circRNA_100395 sponges miR-142-3p to inhibit gastric cancer progression by targeting the PI3K/AKT axis.
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Cheng, Zhiyi, Liu, Guiyuan, Huang, Chuanjiang, and Zhao, Xiaojun
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CIRCULAR RNA ,STOMACH cancer ,CANCER invasiveness ,LYMPHATIC metastasis ,CELL migration - Abstract
Gastric cancer (GC) has a high morbidity and mortality, hence it is very important to elucidate the molecular pathogenesis mechanism of GC progression in order to find new treatment strategies. The present study aimed to explore the biological function of circular RNA_100395 (circRNA_100395) in GC. The expression level of circRNA_100395 in GC tissues, as well as normal epithelial cells and various gastric cancer cell lines, was detected using reverse transcription-quantitative PCR. Cell Counting Kit-8, EdU assay, flow cytometry and Transwell assays were performed to investigate cell proliferation, apoptosis, migration and invasion, respectively. A dual-luciferase reporter assay was performed to detect the correlation between circRNA_100395 and micro (mi)RNA-142-3p. Western blotting was performed to elucidate the potential regulatory mechanism. circRNA_100395 expression was found to be increased in GC tissues and cell lines. However, miR-142-3p expression was significantly reduced. Besides, low expression levels of circRNA_100395 were associated with poor tumor differentiation, advanced Tumor-Node-Metastasis stage, lymph node metastasis and shorter overall survival time. Moreover, overexpression of circRNA_100395 suppressed cell proliferation, increased the apoptosis rate and suppressed cell invasion and migration by inhibiting the PI3K/AKT signaling pathway. These findings also showed that miRNA-142-3p rescued the antitumor effects induced by circRNA_100395-overexpression. cirRNA_100395-overexpression had antitumor effects via regulating the miR-142-3p signaling pathway, which might be a promising treatment target for GC. [ABSTRACT FROM AUTHOR]
- Published
- 2021
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22. LPS-stimulated RAW264.7 macrophage CAT-2–mediated l-arginine uptake and nitric oxide biosynthesis is inhibited by omega fatty acid lipid emulsion
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Huang, Qian, Huang, Chuanjiang, Zhao, Yunzhao, Wang, Bin, Ren, Jianan, Li, Ning, and Li, Jieshou
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LIPOPOLYSACCHARIDES , *MACROPHAGE activation , *AMINO acid transport , *ARGININE , *NITRIC-oxide synthase inhibitors , *OMEGA-3 fatty acids , *EMULSIONS (Pharmacy) , *REVERSE transcriptase polymerase chain reaction - Abstract
Abstract: Background: Omega-3 fatty acid (ω-3 FA) lipid emulsion has been reported to inhibit nitric oxide (NO) production and alter inducible nitric oxide synthase (iNOS) protein expression in lipopolysaccharide (LPS)-stimulated murine macrophages. However, the role of cellular uptake of l-arginine and iNOS transcription in ω-3 FA emulsion-induced inhibition of NO has not been explored. In addition, cationic amino acid transporter-2 (CAT-2) can regulate iNOS activity. The effect of ω-3 FA emulsion on CAT-2 expression is unknown. In the present study, we hypothesized that ω-3 FA emulsion pretreatment would decrease the production of NO in LPS-stimulated macrophages and that this effect would occur through alterations in the cellular uptake of l-arginine and CAT-2 expression, in addition to iNOS expression. Methods: Confluent immortalized murine macrophages (RAW264.7cells) were incubated with Dulbecco’s modified Eagle’s medium, ω-3 FA emulsion, or an isoenergetic ω-6 lipid emulsion for 4 h. The cells were washed and then stimulated with LPS (1 μg/mL) or media alone for 12 or 24 h before harvesting. Greiss reagent was used to assess NO production of plate well supernatants. Cellular uptake of l-arginine was assessed through [3H]-l-arginine. The expression of iNOS and CAT-2 mRNA in harvested RAW264.7 was quantified by reverse transcriptase-polymerase chain reaction. Results: NO production of unstimulated RAW264.7 cells was similar in all groups. After LPS stimulation, ω-3 FA pretreatment at 12 and 24 h produced significantly less NO (P < 0.05) compared with ω-6 FA or media only. ω-3 FA pretreatment at 12 and 24 h resulted in less l-arginine uptake. iNOS and CAT-2 mRNA was significantly decreased with ω-3 FA pretreatment compared with ω-6 FA or media-only treatment (P < 0.05). Conclusions: These experiments demonstrated that, in addition to other anti-inflammatory effects, ω-3 FA lipid emulsion also significantly lowers NO production and l-arginine transport through altered expression of iNOS and CAT-2 in LPS-stimulated RAW264.7 macrophage cells. [Copyright &y& Elsevier]
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- 2013
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23. Galectin-1 promotes gastric cancer peritoneal metastasis through peritoneal fibrosis.
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Shen X, Liu H, Zhou H, Cheng Z, Liu G, Huang C, Dou R, Liu F, and You X
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- Animals, Humans, Peritoneum pathology, Galectin 1 genetics, Peritoneal Fibrosis genetics, Peritoneal Fibrosis metabolism, Peritoneal Neoplasms secondary, Stomach Neoplasms pathology
- Abstract
Background: Peritoneal metastasis is one of the main causes of death in patients with gastric cancer (GC). Galectin-1 regulates various undesirable biological behaviors in GC and may be key in GC peritoneal metastasis., Methods: In this study, we elucidated the regulatory role of galectin-1 in GC cell peritoneal metastasis. GC and peritoneal tissues underwent hematoxylin-eosin (HE), immunohistochemical (IHC), and Masson trichrome staining to analyze the difference in galectin-1 expression and peritoneal collagen deposition in different GC clinical stages. The regulatory role of galectin-1 in GC cell adhesion to mesenchymal cells and in collagen expression was determined using HMrSV5 human peritoneal mesothelial cells (HPMCs). Collagen and corresponding mRNA expression were detected with western blotting and reverse transcription PCR, respectively. The promoting effect of galectin-1 on GC peritoneal metastasis was verified in vivo. Collagen deposition and collagen I, collagen III, and fibronectin 1 (FN1) expression in the peritoneum of the animal models were detected by Masson trichrome and IHC staining., Results: Galectin-1 and collagen deposition in the peritoneal tissues was correlated with GC clinical staging and were positively correlated. Galectin-1 enhanced the ability of GC cells to adhere to the HMrSV5 cells by promoting collagen I, collagen III, and FN1 expression. The in vivo experiments confirmed that galectin-1 promoted GC peritoneal metastasis by promoting peritoneal collagen deposition., Conclusion: Galectin-1-induced peritoneal fibrosis may create a favorable environment for GC cell peritoneal metastasis., (© 2023. The Author(s).)
- Published
- 2023
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24. Galectin-1-mediated high NCAPG expression correlates with poor prognosis in gastric cancer.
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Zheng T, Qian T, Zhou H, Cheng Z, Liu G, Huang C, Dou R, Liu F, and You X
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- Humans, Galectin 1 genetics, Galectin 1 metabolism, Prognosis, Cell Movement genetics, Cell Proliferation, Gene Expression Regulation, Neoplastic, Cell Line, Tumor, Cell Cycle Proteins metabolism, Stomach Neoplasms pathology
- Abstract
Galectin-1 (Gal1) and non-SMC condensin I complex, subunit G (NCAPG) are associated with metastasis in several malignant tumors. However, their precise roles in gastric cancer (GC) remain uncertain. This study explored the clinical significance and relationship of Gal1 and NCAPG in GC. Gal1 and NCAPG expressions were significantly up-regulated in GC compared to adjacent non-cancerous tissues by immunohistochemistry (IHC) and Western blotting. Besides, methods including stable transfection, quantitative real-time reverse transcription PCR, Western blotting, Matrigel invasion and wound-healing assays in vitro , were also conducted. IHC scores for Gal1 and NCAPG had a positive correlation in GC tissues. High Gal1 or NCAPG expression significantly correlated with poor prognosis in GC, and Gal1 combined with NCAPG had a synergetic effect on the prediction of GC prognosis. Gal1 overexpression in vitro enhanced NCAPG expression, cell migration, and invasion in SGC-7901 and HGC-27 cells. Simultaneous Gal1 overexpression and NCAPG knockdown in GC cells partly rescued the migrative and invasive abilities. Thus, Gal1 promoted GC invasion through increased NCAPG expression. The present study demonstrated the prognostic significance of the combination of Gal1 and NCAPG in GC for the first time.
- Published
- 2023
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25. Passage of exogeneous fine particles from the lung into the brain in humans and animals.
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Qi Y, Wei S, Xin T, Huang C, Pu Y, Ma J, Zhang C, Liu Y, Lynch I, and Liu S
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- Animals, Blood, Humans, Mice, Particle Size, Tissue Distribution, Brain metabolism, Lung chemistry, Lung metabolism, Particulate Matter analysis, Particulate Matter blood, Particulate Matter chemistry, Particulate Matter metabolism
- Abstract
There are still significant knowledge gaps in understanding the intrusion and retention of exogeneous particles into the central nervous system (CNS). Here, we uncovered various exogeneous fine particles in human cerebrospinal fluids (CSFs) and identified the ambient environmental or occupational exposure sources of these particles, including commonly found particles (e.g., Fe- and Ca-containing ones) and other compositions that have not been reported previously (such as malayaite and anatase TiO
2 ), by mapping their chemical and structural fingerprints. Furthermore, using mouse and in vitro models, we unveiled a possible translocation pathway of various inhaled fine particles from the lung to the brain through blood circulation (via dedicated biodistribution and mechanistic studies). Importantly, with the aid of isotope labeling, we obtained the retention kinetics of inhaled fine particles in mice, indicating a much slower clearance rate of localized exogenous particles from the brain than from other main metabolic organs. Collectively, our results provide a piece of evidence on the intrusion of exogeneous particles into the CNS and support the association between the inhalation of exogenous particles and their transport into the brain tissues. This work thus provides additional insights for the continued investigation of the adverse effects of air pollution on the brain.- Published
- 2022
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26. Duodenal-Distal Ileal Fistula After Laparoscopic Radical Right Hemicolectomy: A Case Report.
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You X, Zhao X, Huang C, Cheng Z, Liu G, Shen X, and Zheng T
- Abstract
Right hemicolectomy for colon cancer may be complicated by leaks, stenoses, or fistulas. These complications usually occur at the ileocolic anastomosis and can be managed endoscopically. However, fistulas that are large cannot be managed by endoscopy and require surgical intervention. After laparoscopic radical right hemicolectomy, duodenal fistulae is relatively rare. Among duodenal fistulae, internal duodenocolic fistulae is relatively common, but duodeno-ileum fistulae is extremely rare. Here, we report a case of duodeno-distal ileum fistula after right hemicolectomy with short bowel syndrome, that was surgically treated. After surgical treatment, the symptoms of short bowel syndrome disappeared, weight gain was obvious, and the clinical effect was satisfactory., Competing Interests: The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest., (Copyright © 2022 You, Zhao, Huang, Cheng, Liu, Shen and Zheng.)
- Published
- 2022
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27. Steroid Receptor Coactivator-1 (SRC-1) Promoted Cell Metastasis of Gastric Cancer via VEGFC Activator by NF-κB.
- Author
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Zhao X, You X, Huang C, Liu G, Cheng Z, and Zhang H
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- Cell Line, Tumor, Humans, NF-kappa B metabolism, Signal Transduction, Vascular Endothelial Growth Factor C genetics, Vascular Endothelial Growth Factor C metabolism, Nuclear Receptor Coactivator 1 blood, Receptors, Steroid, Stomach Neoplasms metabolism
- Abstract
The regulatory mechanism and function of steroid receptor coactivator-1 (SRC-1) was determined in vitro and the role played in gastric cancer was investigated. The study collected 64 patients with gastric cancer tissue and paracancerous tissue to investigate the clinical patterns of SRC-1 expression in gastric cancer. Quantitative polymerase chain reaction, Western blot, enzyme-linked immunosorbent assay, and immunofluorescence staining were used in this study. In patients with gastric cancer, SRC-1 serum expression levels were up-regulated. Over-expression of SRC-1 promoted cell growth and cell metastasis in vitro model of gastric cancer. However, down-regulation of SRC-1 reduced cell growth and cell metastasis in vitro model of gastric cancer. SRC-1 over-expression induced vascular endothelial growth factor C (VEGFC) protein expressions in vitro model by activation of nuclear factor-kappa B (NF-kB) expression. The inhibition of NF-κB reduced the pro-cancer effects of SRC-1 on cell growth and cell metastasis in vitro model of gastric cancer through inhibition of VEGFC expression. These results suggest that SRC-1 promoted cell metastasis of gastric cancer via VEGFC activator by NF-κB. These novel findings may shed further light on the pathogenesis of gastric cancer and on potential precursor markers.
- Published
- 2022
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28. Fibroblastic galectin-1-fostered invasion and metastasis are mediated by TGF-β1-induced epithelial-mesenchymal transition in gastric cancer.
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You X, Wu J, Zhao X, Jiang X, Tao W, Chen Z, Huang C, Zheng T, and Shen X
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- Adult, Aged, Aged, 80 and over, Cell Line, Tumor, Cell Movement, Epithelial-Mesenchymal Transition, Female, Gene Expression Regulation, Neoplastic, Humans, Male, Middle Aged, Neoplasm Invasiveness, Neoplasm Staging, Prognosis, Signal Transduction, Smad3 Protein metabolism, Stomach Neoplasms pathology, Cancer-Associated Fibroblasts metabolism, Galectin 1 metabolism, Stomach Neoplasms metabolism, Transforming Growth Factor beta1 metabolism
- Abstract
Background The gastric cancer (GC) microenvironment has important effects on biological behaviors, such as tumor cell invasion and metastasis. However, the mechanism by which the GC microenvironment promotes GC cell invasion and metastasis is unknown. The present study aimed to clarify the effects and mechanism of galectin-1 (GAL-1, encoded by LGALS1 ) on GC invasion and metastasis in the GC microenvironment. Methods The expression of GAL-1/ LGALS1 was determined using western blotting, immunohistochemistry, and quantitative real-time reverse transcription PCR in GC tissues. Besides, methods including stable transfection, Matrigel invasion and migration assays, and wound-healing assays in vitro ; and metastasis assays in vivo , were also conducted. Results GAL-1 from cancer-associated fibroblasts (CAFs) induced the epithelial-mesenchymal transition (EMT) of GC cells though the transforming growth factor beta (TGF-β1)/ Sma- and mad-related protein (Smad) pathway, and affected the prognosis of patients with GC. The level of GAL-1 was high in CAFs, and treating MGC-803 and SGC -7901 cell line with the conditioned medium from CAFs promoted their invasion and metastasis abilities. Overexpression of LGALS1 promoted the expression of TGF-β1 and induced EMT of GC cell lines. A TGF-β1 antagonist inhibited the invasion and migration of GC cells. In vivo , overexpression of LGALS1 promoted GC growth and metastasis, and the TGF-β1 antagonist dramatically reversed these events. Conclusions These findings suggested that high expression of GAL-1 in the GC microenvironment predicts a poor prognosis in patients with GC by promoting the migration and invasion of GC cells via EMT through the TGF-β1/Smad signaling pathway. The results might provide new therapeutic targets to treat GC.
- Published
- 2021
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29. Galectin-1 promotes vasculogenic mimicry in gastric adenocarcinoma via the Hedgehog/GLI signaling pathway.
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You X, Wu J, Wang Y, Liu Q, Cheng Z, Zhao X, Liu G, Huang C, Dai J, Zhou Y, Chen D, and Chong Y
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- Adenocarcinoma genetics, Adenocarcinoma pathology, Animals, Cell Line, Tumor, Galectin 1 genetics, Gene Expression Regulation, Neoplastic, Humans, Male, Mice, Nude, Signal Transduction, Stomach Neoplasms genetics, Stomach Neoplasms pathology, Zinc Finger Protein GLI1 genetics, Adenocarcinoma metabolism, Galectin 1 metabolism, Hedgehog Proteins metabolism, Molecular Mimicry, Neovascularization, Pathologic, Stomach Neoplasms metabolism, Zinc Finger Protein GLI1 metabolism
- Abstract
Background: Galectin-1 (GAL-1), which is encoded by LGALS1 , promotes vasculogenic mimicry (VM) in gastric cancer (GC) tissue. However, the underlying mechanism remains unclear., Methods: Immunohistochemical (IHC) and CD34-periodic acid-Schiff (PAS) double staining were used to investigate Glioma-associated oncogene-1(GLI1) expression and VM in paraffin-embedded sections from 127 patients with GC of all tumor stages. LGALS1 or GLI1 were stably transduced into MGC-803 cells and AGS cells, and western blotting, IHC, CD34-PAS double staining and three-dimensional culture in vitro, and tumorigenicity in vivo were used to explore the mechanisms of GAL-1/ GLI1 promotion of VM formation in GC tissues., Results: A significant association between GAL-1 and GLI1 expression was identified by IHC staining, as well as a significant association between GLI1 expression and VM formation. Furthermore, overexpression of LGALS1 enhanced expression of GLI1 in MGC-803 and AGS cells. GLI1 promoted VM formation both in vitro and in vivo. The effects of GLI1 on VM formation were independent of LGALS1 . Importantly, the expression of VM-related molecules, such as MMP2, MMP14 and laminin5γ2, was also affected upon GLI1 overexpression or silencing in GC cell lines., Conclusion: GAL-1 promotes VM in GC through the Hh/GLI pathway, which has potential as a novel therapeutic target for treatment of VM in GC.
- Published
- 2020
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30. [Application of double-pouch anastomosis in laparoscopic radical resection of rectal cancer assisted by small incision].
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You X, Wu J, Wang Y, Zhao X, Zhou Y, Li W, Xv N, Cheng Z, Huang C, and Liu G
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- Humans, Retrospective Studies, Treatment Outcome, Anastomosis, Surgical standards, Laparoscopy, Rectal Neoplasms surgery
- Abstract
Objective: To explore the feasibility, safety and the economical efficiency of double-pouch anastomosis in laparoscopic radical rectal cancer assisted by small incisions., Methods: Clinical data of 224 patients undergoing gastrointestinal surgery at Taizhou People's Hospital of Jiangsu Province from January 2011 to December 2017 were retrospectively analyzed. Indusion criteria: patients were diagnosed as primary rectal adenocarcinoma by preoperative enteroscopy pathology, the distance of the tumor to anal margin was from 4 to 15 cm, and patients were treated with laparoscopic total mesorectal excision(TME) through small incision. Patients were divided into two groups according to different anastomosis method, double-pouch group(108 cases) and single-pouch group (116 cases). The surgical indexes, tumor safety indexes, short-term efficacy and economic indexes were compared between the two groups., Results: There was no significant difference between two groups in baseline data, operative time, blood loss, number of lymph nodes dissection, average length of proximal and distal bowel, or incidence of urination and sexual dysfunction (all P>0.05). Compared with the single-pouch group, the double-pouch group presented lower anastomotic secondary bleeding rate [0.9%(1/108) vs. 6.0% (7/116), χ²=4.238, P=0.040], lower incidence of anastomotic leakage[1.9%(2/108) vs. 7.8%(9/116), χ²=4.179, P=0.041], lower incidence of anastomotic stricture [1.9% (2/108) vs. 8.6% (10/116), χ²=5.054, P=0.025], shorter hospital stay [(13.4±3.9) days vs. (15.9±9.8) days, t=2.524, P=0.013] and less average hospitalization costs [(34 000±7 000) yuan vs. (46 000±23 000) yuan, t=5.047,P<0.001]. There was no significant difference in local recurrence, distant metastasis or overall survival between the two groups during mean follow-up of 33 months (all P>0.05)., Conclusion: Laparoscopic TME assisted by small incision with double-pouch anastomosis is a safe, feasible and economical method.
- Published
- 2018
31. [Clinical study of preserving left colic artery during laparoscopic total mesorectal excision for the treatment of rectal cancer].
- Author
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You X, Wang Y, Chen Z, Li W, Xu N, Liu G, Zhao X, and Huang C
- Subjects
- Humans, Neoplasm Recurrence, Local, Treatment Outcome, Laparoscopy methods, Mesenteric Artery, Inferior surgery, Rectal Neoplasms surgery
- Abstract
Objective: To evaluate the feasibility, safety, radicality and short-term outcome of preserving left colic artery (LCA) during laparoscopic total mesorectal excision (TME) for the treatment of rectal cancer., Methods: From January 2013 to December 2016,136 patients with mid-lower rectal cancer received laparoscopic TME in the Gastrointestinal Surgery Department of Taizhou People's Hospital of Jiangsu Province. Patients with rectal tumor within 10 cm to the anal verge were enrolled into the study. All the enrolled patients had complete data of pathology and follow-up. Those receiving neoadjuvant chemoradiotherapy, with severe base diseases, multifocal tumor, tumor invasion of surrounding tissues, fixation of tumor, recurrent tumor, complications such as acute ileus, bleeding, perforation were excluded. In this study, 72 patients did not undergo preservation of LCA (high ligation group) and 64 patients underwent preservation of LCA (low ligation group). Operative parameters, clinicopathological data and short-term outcome were collected and compared between two groups., Results: The baseline data including gender, age, body mass index, tumor stage, and distance of tumor from anal verge of two groups were comparable (P>0.05). The differences between two groups about the mean time of operation and the operative blood loss were not significant [(164.0±12.6) min vs. (167.3±9.4) min, (30.0±3.6) ml vs. (30.1±3.0) ml, all P>0.05]. There was no operative death in both groups. Differences in the lymph node dissection (13.7±2.6 vs. 13.3±2.1) and the specimen length of proximal resection margin [(16.4±1.9) cm vs. (16.7±2.1) cm] or distal resection margins [(3.9±0.6) cm vs. (4.1±0.9) cm] between high and low ligation groups were not significant (all P>0.05). Compared with high ligation group, the low ligation group had higher rate of sphincter preservation [92.2% (59/64) vs. 79.2% (57/72), χ
2 =4.580, P=0.032], lower rate of anastomotic leakage [1.6% (1/64) vs. 9.7% (7/72), χ2 =4.075, P=0.044], anastomotic stenosis [3.1% (2/64) vs. 12.5%(9/72), χ2 =4.006, P=0.045], and voiding and sexual dysfunction [6.3%(4/64) vs. 18.1%(13/72), χ2 =4.317, P=0.038]. Mean time of follow-up was 19 months. In high ligation group, the local recurrent rate was 5.56%, distant metastasis rate was 13.89%, overall survival rate was 90.28%, disease-free survival rate was 80.56%, while in low ligation group, the local recurrence rate was 4.69%, distant metastasis rate was 12.50%, overall survival rate was 90.63%, disease-free survival rate was 82.81%, whose differences between two groups were not significant (all P>0.05)., Conclusion: Preservation of LCA during laparoscopic TME for the treatment of rectal cancer is safe and feasible, which can reduce the incidence of anastomotic leakage and stenosis, and voiding and sexual dysfunction.- Published
- 2017
32. [Clinical significance of No.12 lymph node dissection for advanced gastric cancer].
- Author
-
You X, Wang Y, Li W, Zhao X, Cheng Z, Xu N, Huang C, and Liu G
- Subjects
- Antigens, Tumor-Associated, Carbohydrate blood, CA-19-9 Antigen blood, Carcinoembryonic Antigen blood, Female, Follow-Up Studies, Humans, Lymph Nodes surgery, Lymphatic Metastasis pathology, Lymphatic Metastasis physiopathology, Male, Middle Aged, Multivariate Analysis, Neoplasm Grading statistics & numerical data, Neoplasm Invasiveness, Neoplasm Staging statistics & numerical data, Prognosis, Retrospective Studies, Risk Factors, Stomach Neoplasms mortality, Survival Rate, Lymph Node Excision methods, Lymph Nodes pathology, Lymphatic Metastasis diagnosis, Stomach Neoplasms blood, Stomach Neoplasms pathology
- Abstract
Objective: To evaluate the clinical significance of No.12 lymph node dissection for advanced gastric cancer with D2 lymphadenectomy., Methods: Clinicopathologic data and No.12 lymph node dissection of 256 advanced gastric cancer patients undergoing radical operation in our department between January 2005 and December 2010 were retrospectively summarized and the influence factors of metastasis in No.12 lymph nodes were analyzed., Results: Of 256 patients, 179 were male and 77 were female with the average age of 59.2 years. Tumor located in the upper of stomach in 24 cases, middle of stomach in 41 cases, lower of stomach in 174 cases, multi-focus or diffuse distribution of stomach in 17 cases. Tumor diameter was <3 cm in 39 cases, 3 to 5 cm in 100 cases, >5 cm in 117 cases. Serum carcinoembryonic antigen (CEA) level increased in 61 cases, serum carbohydrate antigens (CA)72-4 increased in 56 cases and CA19-9 increased in 61 cases. The number of No.12 lymph nodes resected from all the patients was 1 152, and the average number was 4.5±1.9. The metastasis rate of No.12 lymph nodes was 9.4%(24/256) after hematoxylin eosin staining (positive group). All the patients received effective follow-up to December 2015, and the average follow-up time was 101.2 months. The median survival time of positive No.12 group (24 cases) was 29.8 months and of negative No.12 group (232 cases) was 78.2 months, whose difference was statistically significant (χ
2 =21.715, P=0.000). Univariate analysis found that No.12 lymph node metastasis was not associated with age, gender, tumor differentiation (all P>0.05), but was associated with tumor location, tumor diameter, invasive depth (all P<0.05), and was closely associated with Borrmann type, outside metastatic lymph nodes of No.12 and high levels of serum CEA, CA72-4 and CA19-9 (all P=0.000). Multivariate regression analysis found that tumor location (RR=2.452, 95%CI:1.537 to 3.267, P=0.000), Borrmann type (RR=1.864, 95%CI:1.121 to 3.099, P=0.016) and number of outside metastatic lymph nodes of No.12 (RR=2.979, 95%CI: 2.463 to 3.603, P=0.000) were the independent risk factors of the No.12 metastasis (P<0.05)., Conclusions: Metastasis in No.12 lymph nodes indicates poorer prognosis. The No.12 lymph nodes of advanced gastric cancer patients with curative resection, especially those with the tumor located in the lower part, Borrmann type IIII(, outside metastatic lymph nodes of No.12, should be regularly cleaned.- Published
- 2017
33. [Application study on regional infusion chemotherapy by celiac trunk during operation in advanced gastric cancer patients].
- Author
-
You X, Qian H, Qin L, Wang Y, Li W, Lian Y, Zhao X, Xu N, Huang C, Chen Z, and Liu G
- Subjects
- Antineoplastic Combined Chemotherapy Protocols therapeutic use, Capecitabine, Celiac Artery, Chemotherapy, Cancer, Regional Perfusion adverse effects, Cisplatin adverse effects, Deoxycytidine analogs & derivatives, Deoxycytidine therapeutic use, Disease-Free Survival, Fluorine adverse effects, Fluorouracil analogs & derivatives, Fluorouracil therapeutic use, Gastrectomy, Humans, Leucovorin therapeutic use, Lymph Node Excision, Organoplatinum Compounds therapeutic use, Oxaliplatin, Oxaloacetates, Postoperative Complications, Recovery of Function, Survival Rate, Chemotherapy, Cancer, Regional Perfusion methods, Chemotherapy, Cancer, Regional Perfusion mortality, Cisplatin administration & dosage, Cisplatin therapeutic use, Fluorine administration & dosage, Fluorine therapeutic use, Neoplasm Recurrence, Local prevention & control, Stomach Neoplasms drug therapy, Stomach Neoplasms mortality, Stomach Neoplasms surgery
- Abstract
Objective: To explore the feasibility, safety and efficacy of intraoperative regional infusion chemotherapy by celiac trunk in advanced gastric cancer patients., Methods: One hundred and twenty-six patients with advanced gastric cancer(stageII(-III() were screened from database of Gastrointestinal Surgery Department of Taizhou People's Hospital between January 2008 and December 2010 who underwent R0 resection and D2 lymphadenectomy, received postoperative chemotherapy(XELOX or FOLFOX), and had complete follow-up data. They were divided into infusion chemotherapy group (65 cases) and control group (61 cases) according to regional infusion chemotherapy or not (fluorine 1 000 mg and cisplatin 60 mg). The side effects of chemotherapy, parameters related to the operation, long-term survival and relapse rate were compared between the two groups., Results: The baseline data between the two groups were comparable(all P>0.05). Postoperative III( and IIII( adverse reaction of chemotherapy was not significantly different between the two groups (P>0.05). The time of postoperative intestinal function recovery [(67.9±14.8) hours vs. (68.9±15.0) hours, t=-0.380, P=0.705), volume of postoperative 1-week drainage [(66.1±17.1) ml vs.(61.9±18.2) ml, t=1.478, P=0.142], recent morbidity of complications[55.4%(36/65) vs. 49.2%(30/61), χ
2 =0.256, P=0.613], and the long-term morbidity of complications [16.9% (11/65) vs. 14.8% (9/61), χ2 =0.111, P=0.739] were all not significantly different between the two groups. The 3-year survival rate and 3-year relapse-free survival rate in infusion chemotherapy group were significantly higher than those in control group(58.4% vs. 37.7%, χ2 =5.382, P=0.020; 58.4% vs. 34.4%, χ2 =6.636, P=0.010)., Conclusion: Regional infusion chemotherapy by celiac trunk during operation for advanced gastric cancer patients is safe and feasible, and can reduce the risk of local recurrence and improve survival rate.- Published
- 2016
34. Maresin 1 ameliorates iron-deficient anemia in IL-10(-/-) mice with spontaneous colitis by the inhibition of hepcidin expression though the IL-6/STAT3 pathway.
- Author
-
Wang H, Shi P, Huang C, and Liu Q
- Abstract
Background: Approximately 50% of patients with inflammatory bowel disease (IBD) suffer from anemia, which is prevalently caused by iron deficiency. Maresin 1 (MaR1) is a novel docosahexaenoic acid-derived pro-resolving agent that promotes the resolution of inflammation. The aim of the present study was to investigate the therapeutic effects of MaR1 on iron-deficient anemia in IL-10 knockout (IL-10(-/-)) mice with spontaneous chronic colitis., Methods: IL-10(-/-) mice of 16 weeks of age with established colitis were used for the experiments with MaR1 treatment for 2 weeks. Histologic injury, CD4+ lymphocyte values in the lamina propria, blood hemoglobin, hematocrit, serum iron concentrations, transferrin saturation, splenic iron stores, levels of inflammatory cytokines, expression of liver hepcidin mRNA, and western blotting of STAT3 were analyzed in this study., Results: MaR1 treatment (0.3 ng/mouse) effectively attenuated histological colitis typically associated with decreased CD4+ lymphocytes in the lamina propria as well as the concentrations of MPO, TNF-α, IFN-γ, IL-6 and IL-17 (P<0.05). Furthermore, reduced expression of liver hepcidin mRNA and p-STAT3 expression, as well as increased hemoglobin concentration, hematocrit, levels of serum iron, transferrin saturation and splenic iron stores were found in IL-10(-/-) mice after MaR1 treatment (P<0.05)., Conclusions: These results indicate that MaR1 treatment ameliorates iron-deficient anemia by reducing colonic inflammation and inhibiting hepcidin expression though the IL-6/STAT3 pathway.
- Published
- 2016
35. [Application of protective appendicostomy after sphicter-preserving surgery for patients with low rectal carcinoma who are at high-risk of anastomotic leakage].
- Author
-
You X, Wang Y, Zhao X, Lian Y, Xu N, and Huang C
- Subjects
- Colostomy, Humans, Ileostomy, Length of Stay, Postoperative Complications, Retrospective Studies, Surgical Stomas, Anastomotic Leak, Rectal Neoplasms
- Abstract
Objective: To explore the application of protective appendicostomy after sphicter-preserving surgery for patients with low rectal carcinoma who are at high-risk of anastomotic leakage., Methods: Clinical data of 74 low rectal cancer cases with high-risk anastomotic leakage undergoing laparoscope-assisted total mesorectal excision(TME) sphincter-preserving operation in our department from September 2013 to September 2014 were analyzed retrospectively. Patients were randomly divided into two groups: 36 patients received appendicostomy and catheter was removed 4 to 6 weeks after operation when sinus tract formation in abdominal wall was identified; 38 patients received traditional ileostomy and stoma closure was performed 3 to 6 months after operation., Results: The operation time was (149.2±9.4) min vs. (146.7±12.7) min, postoperative complication morbidity was 8.3%(3/36) vs. 13.2%(5/38), anastomotic leakage rate was 2.8%(1/38) vs. 2.6%(1/36), mean drainage volume of 1-week stoma was (203.2±76.9) ml vs. (195.8±76.5) ml, intestinal function recovery time was (25.5±5.6) h vs. (24.0±5.8) h in intubation colostomy group and ileostomy group respectively, and these differences were not significant (all P>0.05). While total hospital stay was shorter and cost was less in intubation colostomy group as compared to ileostomy group [(8.8±1.7) d vs. (18.0±1.7) d, (32 000±3000) yuan vs. (51 000±4000) yuan], and these differences were significant (all P<0.05)., Conclusion: For low rectal cancer patients who are at high-risk of developing anastomotic leakage undergoing sphincter-preserving anterior resection, protective appendicostomy can decrease anastomotic leakage rate, avoid second stoma closure, shorten hospital stay and reduce hospital cost.
- Published
- 2015
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