29 results on '"Han, Zhi-Peng"'
Search Results
2. Yucca schidigera extract depressed ammonia emission in manure incubation and green-house gases release in artificial rumen of cows
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JIN XIAO, HAN ZHI-PENG, YUAN-YUAN DU, SORGOG MA, and SHI BIN-LIN
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Ammonia ,Green-house gas ,Manure ,Rumen ,Cows ,Agriculture - Abstract
The influences of ammonia (NH3) and greenhouse gas (GHG) emissions from livestock on environment recently become a major concern of the worldwide. The objective of the study was to evaluate the effects of Yucca schidigera extract (YSE) on ammonia emission in manure and the release of green-house gases including methane (CH4), nitrous oxide (N2O) and carbon dioxide (CO2) in artificial rumen of cows. Addition of YSE significantly decreased ammonia nitrogen (NH3-N) concentration (P
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- 2022
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3. miR-200a-3p inhibits the PDGF-BB-induced proliferation of VSMCs by affecting their phenotype-associated proteins.
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Rui-Yuan Zeng, Hong-Yi Jin, Yong-Bo Peng, Wen-Jun Wang, Yuan-Ping Cao, Han-Zhi Peng, Zhi-Cong Qiu, Song-Qing Lai, and Li Wan
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CORONARY artery bypass ,VASCULAR smooth muscle ,PHENOTYPIC plasticity ,INHIBITION of cellular proliferation ,WESTERN immunoblotting - Abstract
Accumulating evidence shows that the abnormal proliferation and migration of vascular smooth muscle cells (VSMCs) can significantly affect the long-term prognosis of coronary artery bypass grafting. This study aimed to explore the factors affecting the proliferation, migration, and phenotypic transformation of VSMCs. First, we stimulated VSMCs with different platelet-derived growth factor-BB (PDGF-BB) concentrations, analyzed the expression of phenotype-associated proteins by Western blotting, and examined cell proliferation by scratch wound healing and the 5-ethynyl-2-deoxyuridine (EdU) assay. VSMC proliferation was induced most by PDGF-BB treatment at 20 ng/mL. miR-200a-3p decreased significantly in A7r5 cells stimulated with PDGF-BB. The overexpression of miR-200a-3p reversed the downregulation of α-SMA (p < 0.001) and the upregulation of vimentin (p < 0.001) caused by PDGF-BB. CCK8 and EdU analyses showed that miR-200a-3p overexpression could inhibit PDGF-BB-induced cell proliferation (p < 0.001). However, flow cytometric analysis showed that it did not significantly increase cell apoptosis. Collectively, the overexpression of miR-200a-3p inhibited the proliferation and migration of VSMCs induced by PDGF-BB, partly by affecting phenotypic transformation-related proteins, providing a new strategy for relieving the restenosis of vein grafts. [ABSTRACT FROM AUTHOR]
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- 2024
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4. Lipopolysaccharide induces the differentiation of hepatic progenitor cells into myofibroblasts constitutes the hepatocarcinogenesis-associated microenvironment
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Liu, Wen-ting, Jing, Ying-ying, Gao, Lu, Li, Rong, Yang, Xue, Pan, Xiao-rong, Yang, Yang, Meng, Yan, Hou, Xiao-juan, Zhao, Qiu-dong, Han, Zhi-peng, and Wei, Li-xin
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- 2020
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5. Lipopolysaccharide promotes tumorigenicity of hepatic progenitor cells by promoting proliferation and blocking normal differentiation
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Li, Xiao-Yong, Yang, Xue, Zhao, Qiu-Dong, Han, Zhi-Peng, Liang, Lei, Pan, Xiao-Rong, Zhu, Jing-Ni, Li, Rong, Wu, Meng-Chao, and Wei, Li-Xin
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- 2017
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6. Babao Dan attenuates acute ethanol-induced liver injury via Nrf2 activation and autophagy
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Yu, Yang, Tian, Zhi-qiang, Liang, Lei, Yang, Xue, Sheng, Dan-dan, Zeng, Jian-xing, Li, Xiao-yong, Shi, Rong-yu, Han, Zhi-peng, and Wei, Li-xin
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- 2019
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7. Lipopolysaccharide supports maintaining the stemness of CD133+ hepatoma cells through activation of the NF-κB/HIF-1α pathway
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Lai, Fo-Bao, Liu, Wen-Ting, Jing, Ying-Ying, Yu, Guo-Feng, Han, Zhi-Peng, Yang, Xue, Zeng, Jian-Xing, Zhang, Hang-Jie, Shi, Rong-Yu, Li, Xiao-Yong, Pan, Xiao-Rong, Li, Rong, Zhao, Qiu-Dong, Wu, Meng-Chao, Zhang, Ping, Liu, Jing-Feng, and Wei, Li-Xin
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- 2016
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8. Synthesis of Azoxy Compounds: from Copper Compounds to Mesoporous Silica‐Encaged Ultrasmall Copper Catalysts.
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Han, Zhi‐Peng, Wang, Shiqi, Sun, Qiming, Xu, Xiao‐Ping, and Ji, Shun‐Jun
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COPPER compounds ,NITROSO compounds ,CATALYTIC activity ,METAL catalysts ,COPPER catalysts ,HETEROGENEOUS catalysis ,NANOPOROUS materials ,MESOPOROUS silica - Abstract
Azoxy compounds have aroused extensive attention due to their unique biological activities, but the chemical synthesis of these compounds often suffers from limitations due to their requirement for stoichiometric oxidants, high costs, and restricted substrate range. Herein, a series of azoxy compounds were constructed via facile coupling reactions by using cost‐effective N‐methoxyformamide and nitroso compounds over Cu‐based catalysts, affording high product yields with excellent tolerance of functional groups. Significantly, the mesoporous silica nanosphere‐encapsulated ultrasmall Cu (Cu@MSN) catalyst was developed via a one‐pot synthetic method and first used for the synthesis of azoxy compounds. As compared with copper salt catalysts, the Cu@MSN catalyst exhibited remarkably enhanced catalytic activity and superior recycling stability. Such a Cu@MSN catalyst overcame the inherent drawbacks of low activity, fast deactivation, and difficult recycling of traditional metal salt catalysts in organic reactions. This work provides a green and efficient method for the construction of azoxy compounds and also creates new prospects for the application of nanoporous materials confined metal catalysts in organic synthesis. [ABSTRACT FROM AUTHOR]
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- 2023
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9. Toll like receptor 4 facilitates invasion and migration as a cancer stem cell marker in hepatocellular carcinoma
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Liu, Wen-Ting, Jing, Ying-Ying, Yu, Guo-feng, Han, Zhi-peng, Yu, Dan-dan, Fan, Qing-Min, Ye, Fei, Li, Rong, Gao, Lu, Zhao, Qiu-Dong, Wu, Meng-Chao, and Wei, Li-Xin
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- 2015
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10. Autophagy contributes to the survival of CD133+ liver cancer stem cells in the hypoxic and nutrient-deprived tumor microenvironment
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Song, Yu-jiao, Zhang, Shan-shan, Guo, Xian-ling, Sun, Kai, Han, Zhi-peng, Li, Rong, Zhao, Qiu-dong, Deng, Wei-jie, Xie, Xu-qin, zhang, Jian-wei, Wu, Meng-chao, and Wei, Li-xin
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- 2013
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11. Yucca schidigera extract depressed ammonia emission in manure incubation and greenhouse gases release in artificial rumen of cows.
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JIN XIAO, HAN ZHI-PENG, YUAN-YUAN DU, SORGOG MA, and SHI BIN-LIN
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GREENHOUSE gases ,NITROUS oxide ,AMMONIA ,COWS ,AEROBIC bacteria ,CARBON dioxide - Abstract
The influences of ammonia (NH
3 ) and greenhouse gas (GHG) emissions from livestock on environment recently become a major concern of the worldwide. The objective of the study was to evaluate the effects of Yucca schidigera extract (YSE) on ammonia emission in manure and the release of green-house gases including methane (CH4 ), nitrous oxide (N2 O) and carbon dioxide (CO2 ) in artificial rumen of cows. Addition of YSE significantly decreased ammonia nitrogen (NH3 -N) concentration (P < 0.01) and increased the percentage of total nitrogen (N) (P < 0.005) in manure incubation. The populations of total aerobic bacteria were significantly decreased by the addition of YSE with 1% and 2% (P < 0.05), while the populations of Lactobacilli were significantly increased in dose-dependent manner (P <0.001) in manure. In 1-12 h and 13-24h of ruminal fermentation in vitro, the yields of CH4 , CO2 and N2 O were dose-dependently reduced by the addition of YSE (P < 0.05). Results showed that YSE inhibit ammonia emission in manure and the release of green-house gases in artificial rumen of cows. [ABSTRACT FROM AUTHOR]- Published
- 2022
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12. Rh(III)-Catalyzed C(sp2)–H functionalization/cyclization cascade of N-carboxamide indole and iodonium reagents for access to indoloquinazolinone derivatives.
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Han, Zhi-Peng, Xu, Meng-Meng, Zhang, Rui-Ying, Xu, Xiao-Ping, and Ji, Shun-Jun
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RING formation (Chemistry) , *COLUMN chromatography , *PRECIOUS metals , *METAL catalysts , *FUNCTIONAL groups , *INDOLE - Abstract
A rhodium-catalyzed synthesis of indoloquinazolinone from a readily available hypervalent iodonium reagent and N-carboxamide indole was developed. The protocol features broad functional group tolerance, mild conditions, and excellent yields. The target products were obtained simply by filtration, without tedious column chromatography. Notably, the noble metal catalyst system can be recycled effectively at least ten times. The strategy may be amenable to industrial production. [ABSTRACT FROM AUTHOR]
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- 2021
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13. CD133+CXCR4+ colon cancer cells exhibit metastatic potential and predict poor prognosis of patients
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Zhang Shan-shan, Han Zhi-peng, Jing Ying-ying, Tao Shuang-fen, Li Tie-jun, Wang Hao, Wang Yang, Li Rong, Yang Yang, Zhao Xue, Xu Xiao-dong, Yu En-da, Rui Yao-cheng, Liu Hou-jia, Zhang Li, and Wei Li-xin
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colorectal cancer ,cancer stem cell ,CXCR4 ,epithelial-mesenchymal transition ,liver metastasis ,Medicine - Abstract
Abstract Background Colorectal cancer (CRC), which frequently metastasizes to the liver, is one of the three leading causes of cancer-related deaths worldwide. Growing evidence suggests that a subset of cells exists among cancer stem cells. This distinct subpopulation is thought to contribute to liver metastasis; however, it has not been fully explored in CRC yet. Methods Flow cytometry analysis was performed to detect distinct subsets with CD133 and CXCR4 markers in human primary and metastatic CRC tissues. The 'stemness' and metastatic capacities of different subpopulations derived from the colon cancer cell line HCT116 were compared in vitro and in vivo. The roles of epithelial-mesenchymal transition (EMT) and stromal-cell derived factor-1 (SDF-1) in the metastatic process were also investigated. A survival curve was used to explore the correlation between the content of CD133+CXCR4+ cancer cells and patient survival. Results In human specimens, the content of CD133+CXCR4+ cells was higher in liver metastases than in primary colorectal tumors. Clonogenic and tumorigenic cells were restricted to CD133+ cells in the HCT116 cell line, with CXCR4 expression having no impact on the 'stemness' properties. We found that CD133+CXCR4+ cancer cells had a high metastatic capacity in vitro and in vivo. Compared with CD133+CXCR4- cells, CD133+CXCR4+ cancer cells experienced EMT, which contributed partly to their metastatic phenotype. We then determined that SDF-1/CXCL12 treatment could further induce EMT in CD133+CXCR4+ cancer cells and enhance their invasive behavior, while this could not be observed in CD133+CXCR4- cancer cells. Blocking SDF-1/CXCR4 interaction with a CXCR4 antagonist, AMD3100 (1,10-[1,4-phenylenebis(methylene)]bis-1,4,8,11 -tetraazacyclotetradecane octahydrochloride), inhibited metastatic tumor growth in a mouse hepatic metastasis model. Finally, a high percentage of CD133+CXCR4+ cells in human primary CRC was associated with a reduced two-year survival rate. Conclusions Strategies targeting the SDF-1/CXCR4 interaction may have important clinical applications in the suppression of colon cancer metastasis. Further investigations on how high expression of CXCR4 and EMT occur in this identified cancer stem cell subset are warranted to provide insights into our understanding of tumor biology.
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- 2012
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14. Toll-like receptor 4 signaling promotes epithelial-mesenchymal transition in human hepatocellular carcinoma induced by lipopolysaccharide
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Jing Ying-Ying, Han Zhi-Peng, Sun Kai, Zhang Shan-Shan, Hou Jing, Liu Yan, Li Rong, Gao Lu, Zhao Xue, Zhao Qiu-Dong, Wu Meng-Chao, and Wei Li-Xin
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Toll-like receptor 4 ,Epithelial-mesenchymal transition ,Lipopolysaccharide ,Human hepatocellular carcinoma ,Medicine - Abstract
Abstract Background The endotoxin level in the portal and peripheral veins of hepatocellular carcinoma (HCC) patients is higher and lipopolysaccharide (LPS), a cell wall constituent of gram-negative bacteria, has been reported to inhibit tumor growth. However, in this study, we found that LPS-induced toll-like receptor 4 (TLR4) signaling was involved in tumor invasion and survival, and the molecular mechanism was investigated, Methods Four HCC cell lines and a splenic vein metastasis of the nude mouse model were used to study the invasion ability of LPS-induced HCC cells and the epithelia-mesenchymal transition (EMT) in vitro and in vivo. A total of 106 clinical samples from HCC patients were used to evaluate TLR4 expression and analyze its association with clinicopathological characteristics Results The in vitro and in vivo experiments demonstrated that LPS could significantly enhance the invasive potential and induce EMT in HCC cells with TLR4 dependent. Further studies showed that LPS could directly activate nuclear factor kappa B (NF-κB) signaling through TLR4 in HCC cells. Interestingly, blocking NF-κB signaling significantly inhibited transcription factor Snail expression and thereby inhibited EMT occurrence. High expression of TLR4 in HCC tissues was strongly associated with both poor cancer-free survival and overall survival in patients. Conclusions Our results indicate that TLR4 signaling is required for LPS-induced EMT, tumor cell invasion and metastasis, which provide molecular insights for LPS-related pathogenesis and a basis for developing new strategies against metastasis in HCC.
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- 2012
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15. Lipopolysaccharide induces the differentiation of hepatic progenitor cells into myofibroblasts via activation of the Hedgehog signaling pathway.
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Pan, Xiao-rong, Jing, Ying-ying, Liu, Wen-ting, Han, Zhi-peng, Li, Rong, Yang, Yang, Zhu, Jing-ni, Li, Xiao-yong, Li, Pei-pei, and Wei, Li-xin
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Normally, hepatic progenitor cells (HPCs) are activated and differentiate into hepatocytes or bile ductular cells to repair liver damage during liver injury. However, it remains controversial whether the abnormal differentiation of HPCs occurs under abnormal conditions. Lipopolysaccharide (LPS), a component of the microenvironment, promotes liver fibrosis. In the present study, HPCs promoted liver fibrosis in rats following carbon tetrachloride (CCl4) treatment. Meanwhile, the LPS level in the portal vein was elevated and played a primary role in the fate of HPCs.In vitro, LPS inhibited the hepatobiliary differentiation of HPCs. Concurrently, HPCs co-cultured with LPS for 2 weeks showed a tendency to differentiate into myofibroblasts (MFs). Thus, we conclude that LPS promotes the aberrant differentiation of HPCs into MFs as a third type of descendant. This study provides insight into a novel differentiation fate of HPCs in their microenvironment, and could thus lead to the development of HPCs for treatment methods in liver fibrosis. [ABSTRACT FROM PUBLISHER]
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- 2017
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16. LPS-induced CXCR4-dependent migratory properties and a mesenchymal-like phenotype of colorectal cancer cells.
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Liu, Wen-Ting, Jing, Ying-Ying, Yan, Fei, Han, Zhi-Peng, Lai, Fo-Bao, Zeng, Jian-Xing, Yu, Guo-Feng, Fan, Qing-Min, Li, Rong, Zhao, Qiu-Dong, Wu, Meng-Chao, and Wei, Li-Xin
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- 2017
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17. Hepatic stellate cell promoted hepatoma cell invasion via the HGF/c-Met signaling pathway regulated by p53.
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Liu, Wen-Ting, Jing, Ying-Ying, Yu, Guo-feng, Chen, Hong, Han, Zhi-peng, Yu, Dan-dan, Fan, Qing-Min, Ye, Fei, Li, Rong, Gao, Lu, Zhao, Qiu-Dong, Wu, Meng-Chao, and Wei, Li-Xin
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- 2016
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18. The Injured Liver Induces Hyperimmunoglobulinemia by Failing to Dispose of Antigens and Endotoxins in the Portal System.
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Liu, Wen Ting, Jing, Ying Ying, Han, Zhi Peng, Li, Xiao Ning, Liu, Yan, Lai, Fo Bao, Li, Rong, Zhao, Qiu-Dong, Wu, Meng-Chao, and Wei, Li-Xin
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LIVER injuries ,ANTIGENS ,ENDOTOXINS ,HEPATIC portal system ,IMMUNOGLOBULIN producing cells ,PLASMA cells - Abstract
Hyperimmunoglobulinemia is frequently observed in patients with chronic liver diseases. However, the exact mechanism underlying the high level of antibody formation is not fully understood. In our study, we provide evidence for the functional role of the liver and the stimulation of plasma cell proliferation in hyperimmunoglobulinemia. We collected sera from patients with chronic liver diseases, and the level of serum immunoglobulins in patients was examined; this was also investigated in animal models of liver cirrhosis and hepatocellular carcinoma. An end-to-side microsurgical portacaval shunt was used to mimic liver dysfunction in rats. We used portal vein serum and inferior vena cava serum to immunize healthy rats and mice in order to confirm the function of the healthy liver in disposing of antigens and endotoxins from the gut. For the analysis of the state of plasma cell activation, plasma cells from mice were stained with PE-conjugated anti-CD138 and FITC-conjugated anti-BrdU for flow cytometry analysis. Hyperimmunoglobulinemia was observed both in patients with chronic liver diseases and in related animal models, and high plasma LPS levels were also observed. There was a significant increase in the activation and proliferation of plasma cell in mice immunized with antigens or LPS-positive serum compared with controls that were immunized with antigens and LPS-negative serum. We confirmed that the healthy liver plays an important role in disposing of antigens and endotoxins derived from the gut. Hyperimmunoglobulinemia in chronic liver diseases mainly arises due to the collateral circulation secondary to portal hypertension, gut antigens and endotoxins that bypass the liver and reach the antibody-producing cells. [ABSTRACT FROM AUTHOR]
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- 2015
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19. Mesenchymal stem cells: a new trend for cell therapy.
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Wei, Xin, Yang, Xue, Han, Zhi-peng, Qu, Fang-fang, Shao, Li, and Shi, Yu-fang
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MESENCHYMAL stem cells ,CELLULAR therapy ,APPROXIMATION theory ,IMMUNOLOGIC diseases ,CLINICAL trials ,MEDICATION safety ,DRUG efficacy - Abstract
Mesenchymal stem cells (MSCs), the major stem cells for cell therapy, have been used in the clinic for approximately 10 years. From animal models to clinical trials, MSCs have afforded promise in the treatment of numerous diseases, mainly tissue injury and immune disorders. In this review, we summarize the recent opinions on methods, timing and cell sources for MSC administration in clinical applications, and provide an overview of mechanisms that are significant in MSC-mediated therapies. Although MSCs for cell therapy have been shown to be safe and effective, there are still challenges that need to be tackled before their wide application in the clinic. [ABSTRACT FROM AUTHOR]
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- 2013
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20. Overexpression of SIRT1 promotes metastasis through epithelial-mesenchymal transition in hepatocellular carcinoma.
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Hao, Chong, Zhu, Peng-Xi, Yang, Xue, Han, Zhi-Peng, Jiang, Jing-Hua, Zong, Chen, Zhang, Xu-Guang, Liu, Wen-Ting, Zhao, Qiu-Dong, Fan, Ting-Ting, Zhang, Li, and Wei, Li-Xin
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- 2014
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21. Glycochenodeoxycholate promotes hepatocellular carcinoma invasion and migration by AMPK/mTOR dependent autophagy activation.
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Gao, Lu, Lv, Gang, Li, Rong, Liu, Wen-ting, Zong, Chen, Ye, Fei, Li, Xiao-yong, Yang, Xue, Jiang, Jing-hua, Hou, Xiao-juan, Jing, Ying-ying, Han, Zhi-peng, and Wei, Li-xin
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HEPATOCELLULAR carcinoma , *AUTOPHAGY , *BILE acids , *THERAPEUTICS , *CHOLESTASIS , *CANCER invasiveness , *LIVER cancer - Abstract
Metastasis and recurrence severely impact the treatment effect of hepatocellular carcinoma (HCC). HCC complicated with cholestasis is more prone to recurrence and metastasis. Previous studies have implicated pathogenesis of HCC by bile acid; however, the underlying mechanism is unknown yet. Glycochenodeoxycholate (GCDC) is one of most important component of bile acid (BA). In the present study, the role of GCDC in HCC cells invasion was detected by in vitro and in vivo assays. GCDC was found to significantly enhance the invasive potential of HCC cells; Further studies showed that GCDC could induce autophagy activation and higher invasive capability in HCC cells. Interestingly, inhibition of autophagy by chloroquine (CQ) reversed this phenomenon. Subsequently, the correlation between TBA expression level and clinicopathological characteristics was analyzed in HCC patients. Clinically, high TBA level in HCC tissue was found to be associated with more invasive and poor survival in HCC patients. Mechanistic study showed that bile acid induced autophagy by targeting the AMPK/mTOR pathway in HCC cells. Therefore, our results suggest that bile acid may promote HCC invasion via activation of autophagy and the level of bile acid may serve as a potential useful indicator for prognosis of HCC patients. • GCDC enhanced the metastasis of HCC which was mediated by autophagy. • AMPK/mTOR pathway contributed to the metastatic potential of HCC cells mediated by autophagy. • TBA level was correlated to autophagy activation and HCC prognosis in clinically specimens. [ABSTRACT FROM AUTHOR]
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- 2019
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22. Immune response involved in liver damage and the activation of hepatic progenitor cells during liver tumorigenesis.
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Hou, Xiao-juan, Ye, Fei, Li, Xiao-yong, Liu, Wen-ting, Jing, Ying-ying, Han, Zhi-peng, and Wei, Li-xin
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LIVER cancer , *IMMUNE response , *NEOPLASTIC cell transformation , *HEPATITIS B virus , *CARCINOGENESIS - Abstract
Hepatocellular carcinoma (HCC) is a typical inflammation-related cancer. Hepatitis B virus (HBV) and hepatitis C virus (HCV) infection are well-known leading causes of HCC. However, the mechanism of the induction of HCC by these virus is still being debated. This review will focus on the current knowledge of the pathogenesis of HBV- and HCV-induced inflammation and the role of such immune activation in the tumorigenesis of HCC. It is well established that the recruitment of certain number and type of immune cells to liver is essential for the resolution of HBV and HCV infection and the prevention of subsequent chronic persistent infection. However, in case that the immune response do not completely clear virus, persistent chronic infection occurs, and the perpetual immune response may contribute to chronic damages of the liver. Such chronic inflammatory damages further harm hepatocytes, but not hepatic progenitor cells (HPCs). Thus, following chronic damages, HPCs are activated and their dysregulated proliferation ensures survival in the hostile environment, contributing to the tumorigenesis of HCC. Furthermore, accumulating evidence also provides a strong link between HPCs and human hepatocellular carcinoma. Collectively, these findings support a notion that immune response is involved in liver damage during hepatitis virus infection, and the activation and dysregulated differentiation of hepatic progenitor cells promote the tumorigenesis of human hepatocellular carcinoma. [ABSTRACT FROM AUTHOR]
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- 2018
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23. Babao Dan decreases hepatocarcinogenesis by inhibiting hepatic progenitor cells malignant transformation via down-regulating toll-like receptor 4.
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Liang L, Zhang LY, Liu WT, Zong C, Gao L, Li R, Zhao QD, Zhao NP, Wei LX, Zhang L, and Han ZP
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Background: Babao Dan (BBD) is a traditional Chinese medicine that has been widely used as a complementary and alternative medicine to treat chronic liver diseases. In this study, we aimed to observe the effect of BBD on the incidence of diethylnitrosamine (DEN)-initiated hepatocellular carcinoma formation in rats and explored its possible mechanism., Methods: To verify this hypothesis, BBD was administrated to rats at a dose of 0.5g/kg body weight per two days from the 9th to 12th week in HCC-induced by DEN. Liver injury biomarkers and hepatic inflammatory parameters were evaluated by histopathology as well as serum and hepatic content analysis. We applied immunohistochemical analysis to investigate the expression of CK-19 and SOX-9 in liver tissues. The expression of TLR4 was determined by immunohistochemical, RT-PCR, and western blot analysis. Furthermore, we also detected the efficacy of BBD against primary HPCs neoplastic transformation induced by LPS., Results: We observed that DEN could induce hepatocarcinogenesis, and BBD could obviously decrease the incidence. The biochemical and histopathological examination results confirmed that BBD could protect against liver injury and decrease inflammatory infiltration. Immunohistochemistry staining results showed that BBD could effectively inhibit the ductal reaction and the expression of TLR4. The results showed that BBD-serumcould obviously inhibit primary HPCs neoplastic transformation induced by regulating the TLR4/Ras/ERK signaling pathway., Conclusion: In summary, our results indicate that BBD has potential applications in the prevention and treatment of HCC, which may be related to its effect on hepatic progenitor cells malignant transformation via inhibiting the TLR4/Ras/ERK signaling pathway., Competing Interests: The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest., (Copyright © 2023 Liang, Zhang, Liu, Zong, Gao, Li, Zhao, Zhao, Wei, Zhang and Han.)
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- 2023
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24. IL-1β secreted by macrophage M2 promotes metastasis of osteosarcoma via NF-κB/miR-181α-5p/RASSF1A/Wnt pathway.
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Han ZP, Liu DB, Wu LQ, Li Q, Wang ZG, and Zang XF
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Background: Ras-associated domain family protein1 isoform A (RASSF1A) was significantly absent in clinical samples and many osteosarcoma (OS) cell lines. Overexpression of RASSF1A could suppress OS metastasis, which may be mediated by tumor-associated macrophages polarized M2 (M2-TAMs). However, the relationship between IL-1β secreted by M2-TAMs and RASSF1A remains unknown., Methods: The expression levels of M2-TAMs markers CD68 and CD204 were measured by flow cytometry, and arginase-1 (Arg-1) and interleukin-1β (IL-1β) secreted by M2-TAMs were examined by real-time quantitative PCR (RT-qPCR). MTT assay was employed to determine the proliferation of OS cells, while scratch wound healing assay and Transwell assay were used to evaluate their migration and invasion, respectively. The level of miR-181α-5p was measured by RT-qPCR, while the levels of RASSF1A, GSK-3β, p-GSK-3β, β-catenin, MMP-2 and MMP-9 were evaluated by Western blot. The direct binding of miR-181α-5p and RASSF1A was identified using dual-luciferase reporter assay and RNA immunoprecipitation (RIP) assay., Results: The levels of CD68, CD204, Arg-1 and IL-1β were elevated in M2-TAMs compared with control group. Overexpression of RASSF1A and knockdown of miR-181α-5p could both suppress invasion and migration of OS cells through Wnt pathway. IL-1β secreted by M2-TAMs facilitated the OS metastasis via RASSF1A/Wnt pathway, which could be targeted by miR-181α-5p and affected by nuclear factor-kappa B (NF-κB)., Conclusions: IL-1β secreted by M2-TAMs contributed to OS metastasis, which could be suppressed by knockdown of miR-181α-5p or overexpression of RASSF1A through NF-κB/miR-181α-5p/RASSF1A/Wnt pathway. These findings can guide new target discovery for drug development in OS treatment., Competing Interests: Conflicts of Interest: All authors have completed the ICMJE uniform disclosure form (available at http://dx.doi.org/10.21037/tcr.2020.02.52). The authors have no conflicts of interest to declare., (2020 Translational Cancer Research. All rights reserved.)
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- 2020
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25. Methylation mediated Gadd45β enhanced the chemosensitivity of hepatocellular carcinoma by inhibiting the stemness of liver cancer cells.
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Hou XJ, Zhao QD, Jing YY, Han ZP, Yang X, Wei LX, Zheng YT, Xie F, and Zhang BH
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Background: Defects of the growth arrest DNA damage-inducible gene 45β (Gadd45β) play an important role in the progression of tumor and confer resistance to chemotherapy. However, the role of Gadd45β in the apoptosis of hepatocellular carcinoma is still not clear. Purpose of this study was to explore the effect of Gadd45β on the apoptosis of liver cancer cells, and the possible mechanism was examined., Result: In this study, we first confirmed the decreased expression of Gadd45β in human liver cancer tissues and human liver cancer cell lines, when compared to the peri-tumor liver tissue and normal liver cells. And, it was found that Gadd45β could inhibit the stemness of liver cancer cells, enhancing the apoptosis of cancer cells induced by chemotherapy. Furthermore, the results showed that HCC tissues and cell lines showed a higher methylation status in Gadd45β promoter than that in peri-tumor tissues and normal liver cells. Methylation was then reversed by pretreatment of SMMC-7721 and Hep-3B with 5-azacytidine which is the DNA methyltransferase inhibitor. And the 5-azacytidine decreased the stemness of SMMC-7721 and Hep-3B, enhanced the sensitivity of SMMC-7721 and Hep-3B to cisplatin., Conclusions: Methylation mediated Gadd45β expression inhibited the stemness of liver cancer cells, promoting the chemotherapy-induced apoptosis. Thus Gadd45β may be the potential target for enhancing the chemosensitivity of human hepatocellular carcinoma.
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- 2017
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26. Hepatocyte nuclear factor-1beta enhances the stemness of hepatocellular carcinoma cells through activation of the Notch pathway.
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Zhu JN, Jiang L, Jiang JH, Yang X, Li XY, Zeng JX, Shi RY, Shi Y, Pan XR, Han ZP, and Wei LX
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- Animals, Biomarkers, Tumor metabolism, Carcinoma, Hepatocellular mortality, Carcinoma, Hepatocellular pathology, Carcinoma, Hepatocellular surgery, Cell Dedifferentiation, Cell Line, Tumor, Cell Movement, Epithelial-Mesenchymal Transition genetics, Hepatectomy, Hepatocyte Nuclear Factor 1-beta metabolism, Heterografts, Humans, Liver metabolism, Liver pathology, Liver surgery, Liver Neoplasms mortality, Liver Neoplasms pathology, Liver Neoplasms surgery, Male, Mice, Mice, Inbred BALB C, Mice, Nude, Neoplasm Invasiveness, Neoplastic Stem Cells pathology, Receptor, Notch1 metabolism, Retrospective Studies, Signal Transduction, Survival Analysis, Biomarkers, Tumor genetics, Carcinoma, Hepatocellular genetics, Gene Expression Regulation, Neoplastic, Hepatocyte Nuclear Factor 1-beta genetics, Liver Neoplasms genetics, Neoplastic Stem Cells metabolism, Receptor, Notch1 genetics
- Abstract
Hepatocyte nuclear factor-1beta plays an important role in the development and progression of liver cancer. In recent years, the expression of HNF-1β has been reported to be associated with risk for a variety of cancers. The purpose of this study is to investigate whether the expression of HNF-1β promotes the malignancy of HCC and its mechanism. We retrospectively investigated the expression of HNF-1β in 90 patients with hepatocellular carcinoma and found that the high expression of HNF-1β indicated poor prognosis. We overexpressed HNF-1β in liver cancer cell lines and found the expression of liver progenitor cell markers and stemness were upregulated. The invasion ability and epithelial-mesenchymal transition (EMT)-associated genes were also significantly higher in liver cancer cells overexpressing HNF-1β than in the control group. A mechanistic study suggested the activation of the Notch signalling pathway probably plays a key role downstream of HNF-1β. More importantly, HNF-1β promoted tumourigenesis of HCC cells in vivo. In conclusion, high expression of HNF-1β not only promoted the de-differentiation of HCC cells into liver cancer stem cells through activating the Notch pathway but also enhanced the invasive potential of HCC cells and EMT occurrence, which would contribute to the enhancement of cell migration and invasion.
- Published
- 2017
- Full Text
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27. Hepatitis B virus (HBV) receptors: Deficiency in tumor results in scant HBV infection and overexpression in peritumor leads to higher recurrence risk.
- Author
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Jing YY, Liu WT, Guo SW, Ye F, Fan QM, Yu GF, Yu DD, Gao L, Sun K, Han ZP, Li R, Yang Y, Zhao QD, Wu MC, Wang HY, and Wei LX
- Subjects
- Adolescent, Adult, Aged, Aged, 80 and over, Asialoglycoprotein Receptor analysis, Asialoglycoprotein Receptor biosynthesis, Carcinoma, Hepatocellular mortality, Child, DNA, Viral analysis, Disease-Free Survival, Female, Hepatitis B Surface Antigens analysis, Humans, Immunohistochemistry, In Situ Hybridization, Fluorescence, Inflammation pathology, Inflammation virology, Kaplan-Meier Estimate, Liver Neoplasms mortality, Male, Middle Aged, Neoplasm Recurrence, Local pathology, Organic Anion Transporters, Sodium-Dependent analysis, Organic Anion Transporters, Sodium-Dependent biosynthesis, Symporters analysis, Symporters biosynthesis, Tissue Array Analysis, Young Adult, Carcinoma, Hepatocellular pathology, Carcinoma, Hepatocellular virology, Hepatitis B complications, Liver Neoplasms pathology, Liver Neoplasms virology, Neoplasm Recurrence, Local virology
- Abstract
Hepatitis B virus (HBV) infection is a risk factor for hepatocarcinogenesis and recurrence. Here, we sought to characterize intratumoral and peritumoral expression of HBsAg and its specific receptors in HBsAg-positive hepatocellular carcinoma (HCC) patients and further examined their correlation with the recurrence-free survival (RFS). HCC tissue and adjacent normal tissue specimens were acquired from HBsAg-positive patients. The presence of HBsAg and receptors, as well as hepatic progenitor cells (HPCs) were detected by tissue microassay and immunohistochemistry. Necroinflammatory activity was evaluated by HE staining. The mean IOD of HBsAg and HBV DNA in the intratumoral tissues was markedly lower than that in the peritumoral tissues (P < 0.001). Pearson correlation analysis further showed a significant correlation between the expression of HBsAg and NTCP (r = 0.461, P < 0.001) or ASGPR (r = 0.506, P < 0.001) in peritumoral tissues. And the peritumoral HBsAg and receptors presented a positive association with necroinflammatory activity (P < 0.05). Inflammation induced by HBV infection presented a positive association with HPCs activation (P < 0.05). Additionally, due to lack of HBV receptors, HPCs was not preferentially infected with HBV, but activated HPCs had a significant correlation with HBsAg expression in peritumoral tissues, and the peritumoral HPCs activation was associated with RFS of HCC patients, therefore, the overexpression of HBsAg and receptors in peritumor were also with higher recurrence risk (P < 0.05). In conclusion, lack of HBV receptors resulted in scant HBV infection in tumor cells, and overexpression of HBsAg and receptors in peritumor was strongly associated with higher recurrence risk in HCC patients.
- Published
- 2015
- Full Text
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28. The role of autophagy induced by tumor microenvironment in different cells and stages of cancer.
- Author
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Yang X, Yu DD, Yan F, Jing YY, Han ZP, Sun K, Liang L, Hou J, and Wei LX
- Abstract
Development of a tumor is a very complex process, and invasion and metastasis of malignant tumors are hallmarks and are difficult problems to overcome. The tumor microenvironment plays an important role in controlling tumor fate and autophagy induced by the tumor microenvironment is attracting more and more attention. Autophagy can be induced by several stressors in the tumor microenvironment and autophagy modifies the tumor microenvironment, too. Autophagy has dual roles in tumor growth. In this review, we discussed the interaction between autophagy and the tumor microenvironment and the paradoxical roles of autophagy on tumor growth at different stages of tumor development.
- Published
- 2015
- Full Text
- View/download PDF
29. Effects of inflammatory factors on mesenchymal stem cells and their role in the promotion of tumor angiogenesis in colon cancer.
- Author
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Liu Y, Han ZP, Zhang SS, Jing YY, Bu XX, Wang CY, Sun K, Jiang GC, Zhao X, Li R, Gao L, Zhao QD, Wu MC, and Wei LX
- Subjects
- Animals, Antiviral Agents metabolism, Antiviral Agents pharmacology, Cell Line, Tumor, Chick Embryo, Chorioallantoic Membrane metabolism, Chorioallantoic Membrane pathology, Colonic Neoplasms pathology, Culture Media, Conditioned pharmacology, Hypoxia-Inducible Factor 1, alpha Subunit metabolism, Inflammation Mediators pharmacology, Interferon-gamma pharmacology, Male, Mesenchymal Stem Cells pathology, Mice, Mice, Inbred BALB C, Neovascularization, Pathologic pathology, Vascular Endothelial Growth Factor A metabolism, Colonic Neoplasms metabolism, Inflammation Mediators metabolism, Interferon-gamma metabolism, Mesenchymal Stem Cells metabolism, Neovascularization, Pathologic metabolism, Tumor Microenvironment, Tumor Necrosis Factor-alpha metabolism
- Abstract
Mesenchymal stem cells (MSCs), which are modulated by cytokines present in the tumor microenvironment, play an important role in tumor progression. It is well documented that inflammation is an important part of the tumor microenvironment, so we investigated whether stimulation of MSCs by inflammatory cytokines would contribute to their ability to promote tumor growth. We first showed that MSCs could increase C26 colon cancer growth in mice. This growth-promoting effect was further accelerated when the MSCs were pre-stimulated by inflammatory factors IFN-γ and TNF-α. At the same time, we demonstrated that MSCs pre-stimulated by both inflammatory factors could promote tumor angiogenesis in vivo to a greater degree than untreated MSCs or MSCs pre-stimulated by either IFN-γ or TNF-α alone. A hen egg test-chorioallantoic membrane (HET-CAM) assay showed that treatment of MSC-conditioned medium can promote chorioallantoic membrane angiogenesis in vitro, especially treatment with conditioned medium of MSCs pretreated with IFN-γ and TNF-α together. This mechanism of promoting angiogenesis appears to take place via an increase in the expression of vascular endothelial growth factor (VEGF), which itself takes place through an increase in signaling in the hypoxia-inducible factor 1α (HIF-1α)-dependent pathway. Inhibition of HIF-1α in MSCs by siRNA was found to effectively reduce the ability of MSC to affect the growth of colon cancer in vivo in the inflammatory microenviroment. These results indicate that MSCs stimulated by inflammatory cytokines such as IFN-γ and TNF-α in the tumor microenvironment express higher levels of VEGF via the HIF-1α signaling pathway and that these MSCs then enhance tumor angiogenesis, finally leading to colon cancer growth in mice.
- Published
- 2011
- Full Text
- View/download PDF
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