Search

Aim To evaluate the antimicrobial activity of 0.2% polyhexamethilene biguanide (PHMB) in root canal models infected with Enterococcus faecalis, Candida albicans and Staphylococcus epidermidis. PHMB was compared in these tests with 2.5% NaOCl and 0.2% CHX. Methods Prepared models of 50 human root canals (n=50) were immerged in mixed, four- weeks old culture that consisted of E .faecalis, S. epidermidis and C. albicans. Roots were randomly divided into three groups: one with 30 (n=30) and two with 10 (n=10) samples. Samples were treated with polyhexamethylene biguanide (PHMB) (0.2%), sodium hypochlorite (NaOCl) (2.5%) and chlorhexidine (CHX) (0.2%), respectively. Root dentin was sampled before and after the tretment with these solutions. Colony- forming units (CFU) were counted to asses the antimicrobial effects of three solutions on viability of selected microrganisms in specimens before and after the treatment.T-test was used for comparison of results between specimens before and after the treatment, while Newman-Keuls test was used for pairwise comparison at p=0.05. Results The PHMB was significantly more efficient in reducing the number of all three tested microorganisms. NaOCl and CHX made only statistically significant (p

Objectives: To determine the percentage of persistant apical lesions positive for bacterial nucleic acids, to detect microorganisms difficult to cultivate in persistant apical lesions by PCR and relate them to endodontic failure, clinical symptoms and diabetes mellitus. Materials and methods: The samples of persistent apical lesions were collected during apicoectomy. Bacterial ubiquitous primer 16S rRNA was used to detect 16S ribosomal RNA in 36 samples. A species–specific PCR was performed with primers targeted to the bacterial 16S rRNA genes of Prevotella Nigrescens, Pseudoramibacter alactolyticus, and Propionobacterium propionicum. Results: Six samples (16.67%) were positive for bacterial ribosomal RNA. Pseudoramibacter alactolyticus was detected in three samples. Propionibacterium propionicum and Prevotella nigrescens were detected in one sample each. The prevalence of infection of such lesions with P. intermedia, P. propionicum and P. alactolyticus is low. Conslusion: The study we conducted gave insufficient data about extraradicular infection and its connection with diabetes mellitus and clinical symptoms. Conclusions: Apical lesions persisting after endodontic treatment could harbor microorganisms other than Actinomyces and Propionicum species.

Cilj Ispitati antibakterijski učinak materijala za endodontsku opskrbu zuba. Metode Testom difuzije u agaru ispitivana su punila korijenskih kanala Ketac Endo, AH Plus, Diaket i gutaperka. Od materijala za podloge ispitivani su Cink Oksid/Eugenol cement, staklenoionomerni cementi Fuji II LC Improved i Ketac Cem, te Phosphatcement i Harvard cement. Kao materijali za deinitivne ispune ispitivani su amalgam, Ketac Molar i Fuji II LC Improved. Pri tomesu rabljene bakterije Streptococcus mutans, Streptotoccus mitis,Lactobacillus species, Staphylococcus aureus, Enterococcus faecalis,te polimikrobna suspenzija. Za svaku ispitivanu bakteriju na inokulirane ploče krvnog agara raspoređeni su uzorci materijala. Rezultati Antibakterijski učinak imali su slijedeći materijali: punila kanala - Diaket, AH Plus, Ketac Endo, a za podloge: Cink Oksid/ Eugenol cement, Phosphat i Harvard cement. Antibakterijskiučinak nisu pokazali amalgam, gutaperka, Fuji II LC Improved, Ketac Cem i Ketac Molar. Diaket je pokazao statistički značajno jači antibakterijski učinak naspram AH Plusa, Ketac Endoa i gutaperke(ps.mitis

Aim To evaluate the dye leakage of three flowables with a new‘’three dimensional’’ method.Methods Three groups of twelve premolars have received ClassV cavities of uniform standardized dimensions. The cavities wererestored with: Clearfil liner Bond 2 with adhesive SE Bond (groupI), Definite flow with Etch & Prime 3.0 (group II) and, the Tetricflow with Syntac Single Component (group III). The teeth wereimmersed in a contrast dye for 24 hours, rinsed and immersed inthe 5% nitric acid for 72 hours. The teeth were acid-softened andthe fillings were pulled out of their cavities. The inner surfaces ofextracted fillings were photographed in three different rotated positions(3 x 120 o). The photographs were transferred to a computerimage and the deepest point and the area of the leaked surfacewere determined.Results All restorative systems showed leakage at the adhesivelevel. The statistical analysis showed significant differences betweengroup I and group III in the number of leaked samples, inthe depth of leakage and in the leaked surface. Best results in allthree ways of leakage evaluation were obtained in group I (Clearfilliner Bond 2 with adhesive SE Bond).Conclusion In this study, flowable Clearfil liner Bond 2 with adhesiveSE Bond had leaked in all three ways of evaluation significantllyless than Tetric flow in combination with Syntac SingleComponent.